4Deep HoloSea S7 Manuale utente

HoloSea Microscope User Guide

Model S7

Notices

No part of this document may be reproduced

in any format without prior agreement or writ-

ten consent from 4 Deep inwater imaging.

This software/hardware is proprietary to

4Deep inwater imaging and the company

hereby grants the customer a nonexclusive li-

cense for its use. The Customer will not mod-

ify, adapt, translate, create derivative works,

disassemble, decompile or reverse engineer

the software/hardware provided. No title to

or ownership of the software/hardware or in-

tellectual property rights are transferred to the

customer.

This software/hardware is provided “as is”,

and is subject to change, without further no-

tice in future editions.

Edition

HoloSea Microscope User Guide - Model S7

4Deep inwater imaging

6589 Chebucto Road

Halifax Nova Scotia

Canada B3L 1L9

Safety and Information Notices

An “Important” signiﬁes helpful information in

using the software/hardware. It identiﬁes an

important piece of information to guide the

user in their workﬂow, and if not followed could

result in time wasted.

A “Caution” signiﬁes a hazard. It identiﬁes an

operating procedure, or step, that if not fol-

lowed precisely, could result in damage to the

product or loss of information. Do not continue

beyond a “Caution” sign until the procedure is

fully understood.

A “Warning” signiﬁes a hazard. It identiﬁes

an operating procedure, or step, that if not fol-

lowed precisely, could result in personal injury.

Do not continue beyond a “Warning” sign until

the procedure is fully understood.

HoloSea Microscope

iii

CONTENTS

Contents

1 Overview 1

1.1 Purpose .......................................... 1

1.2 Beneﬁts........................................... 1

2 Introduction 2

2.1 SafetyInformation ..................................... 2

2.2 Compatibility ........................................ 2

2.3 ComponentsoftheSystem ................................ 3

2.4 Recommended Extras (not included) . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.5 The Microscope and its Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.5.1 Chamber1 ..................................... 7

2.5.2 Chamber2 ..................................... 8

2.5.3 SampleSpacer .................................. 8

2.5.4 Crown........................................ 9

2.5.5 PurgeValves .................................... 9

3 Installation and Use 10

3.1 ConnecttheHoloSeacable ................................ 10

3.2 Screwonlockingsleeve.................................. 11

3.3 EthernetConnection.................................... 11

3.4 TestingandDeployment.................................. 13

3.5 CollectingHolograms ................................... 13

3.5.1 Acquisition setup for Octopus and the microscope . . . . . . . . . . . . . . . 14

3.5.2 Tips for Optimal Hologram Acquisition . . . . . . . . . . . . . . . . . . . . . . 16

4 Care and Maintenance 16

5 Troubleshooting 18

6 Appendix 18

6.1 PrincipleofOperation ................................... 18

6.2 Software........................................... 19

7 Speciﬁcations 20

LIST OF FIGURES

List of Figures

1 The preassembled HoloSea Microscope (vertical view in left panel and horizontal

viewinrightpanel). .................................... 4

2 10mCable ......................................... 4

3 PowerSupply(12VDC) .................................. 5

4 Carrying case, with the microscope inside . . . . . . . . . . . . . . . . . . . . . . . . 5

5 HoloSeacomponents ................................... 7

6 Protective sapphire camera window and copper ring. . . . . . . . . . . . . . . . . . . 8

7 Samplespace ....................................... 9

8 The two purge valves on the bottom of the microscope. . . . . . . . . . . . . . . . . 9

9 Mating the underwater connectors for a wet mating (Photo from MacArtney Under-

waterTechnologies) .................................... 10

10 HoloSea bulkhead with 13 pin connector. The male connector is in the left panel,

and is attached to the underwater cable. The female is shown in the right panel and

is attached to the microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

11 Locking sleeve on both the cable (left panel), the microscope (center panel) and

connected(rightpanel) .................................. 11

12 Use of female to female Ethernet connector. In the right panel, the black cord is

the rigid Ethernet cable from the underwater cable, and the blue cable is the ﬂexible

Ethernet cable to the computer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

13 Connectingtothecomputer................................ 12

14 Connecting the power. In the left panel connecting the microscope power to the in-

cluded 12VDC power cord. The right panel shows a close-up of the power connector

fromthemicroscope..................................... 12

15 Deployment of the HoloSea from a wharf . . . . . . . . . . . . . . . . . . . . . . . . 13

16 Dimensions of the Submersible, reported in inches . . . . . . . . . . . . . . . . . . . 13

17 Camera tab view (left panel) and the camera options (right panel) . . . . . . . . . . . 14

18 Select instrument preset dialog . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

19 The Octopus software at start-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

HoloSea S7 1 OVERVIEW

1 Overview

The HoloSea has been developed to operate in conjunction with 4Deep’s software (Octopus,

Swordﬁsh or Stingray). Note that hologram collection cannot be completed without the use of

4Deep software.

This user guide is strictly related to the installation, use, care of and functionality of the HoloSea.

For a detailed description of the setup and operation of the 4Deep software, please refer to the

respective user guides.

The HoloSea is a submersible holographic microscope; for the benchtop microscope, see the

Desktop Microscope.

The HoloSea can be used to collect images from the ﬁelds of:

• Marine research: water proﬁling, algae, plankton, phytoplankton

• Oil & gas

• Water quality and monitoring: microorganism imaging

• Ocean research

• Algae production: algae proﬁling

• Counting, data and morphological analysis, quantitative phase analysis: many other applica-

tions

1.1 Purpose

The HoloSea comes from a suite of instruments that all operate under proprietary digital inline

holography technology platform, developed by 4Deep. With its new and unique method of data

acquisition and analysis, this system is designed to optimize your research of the microscopic

world. The microscope is able to capture dynamic movement and is useful for time-dependent

experiments. The HoloSea allows you to quickly and easily observe micro-organisms and particles

up to a depth of 5 kilometres (in custom models).

The measurement technique used is digital inline holographic microscopy in transmission. The

light source is a single source of 405 nm.

1.2 Beneﬁts

Compared to a traditional microscope, the holographic microscope has many beneﬁts:

• Real-time, in-situ capability to observe of particles and micro-organisms in all aquatic envi-

ronments

• Portable and lightweight, making it easy to deploy, yet rugged

• High-ﬂow capabilities; an be towed at 8 knots (4 m/s), or stationed in rivers

2 INTRODUCTION HoloSea S7

• Low maintenance and easy to clean, as there is no pumps or tubing, making fouling less of

an issue

• Fast-tracking: up to 22 images per second to capture tracking dynamics of fast moving ob-

jects

• Save time using the fastest hologram image reconstruction available

• Go deeper, as it is pressure rated from 0-500m, with a cable depth of 100m, and Up to 6000

meters in custom models

• Instant: live imaging of samples without any manipulation.

• Simple: no need to focus.

2 Introduction

2.1 Safety Information

The user should read this user guide and any other additional information supplied by 4Deep before

operating the instrument.

This microscope contains a class IIIB Laser. A 405 nm wavelength is emitted from the

laser housing itself. If properly assembled, the light emitted from the laser should not pose any

threat of long term damage to an exposed eye. There are no user serviceable parts inside

the light source. If problems are encountered regarding the light source, please return it im-

mediately for service. Allow only a qualiﬁed technician to disassemble the point source. Do not

attempt to remedy any problems with the light source on your own!

Always make sure that the proper 12 VDC power supply is used with the microscope.

Failure to do so could result in critical damage to the microscope.

2.2 Compatibility

As mentioned above, the HoloSea needs to be used in conjunction with the Octopus software. The

computer requirements are:

• CPU Core i5 or i7

• 8 GB of RAM

• 256 GB or larger SSD Drive

–The microscope can record at 22 images per second, and each image is 4 MB

• NVIDIA GeForce or Quadro GPU with at least 2 GB of graphics RAM, Kepler architecture or

later

HoloSea S7 2 INTRODUCTION

–For fast hologram reconstructions, 4Deep software requires a CUDA-enabled NVIDIA

graphics card to be installed in the computer

–For the list of CUDA-enabled graphics chips, refer to https://developer.nvidia.com/cuda-

gpus

• Gigabit Ethernet port (for data connection)

• 1 USB port for the HASP key -Full versions of the software require a valid HASP key (dongle)

to run

–Demo versions can be used without a HASP key

• Windows 7 or 10, 64-bit

–Windows 10 Pro is preferred over other versions (such as Home)

2.3 Components of the System

Be careful when unpacking the contents of the package. As the 4Deep microscope

images at the micron level, foreign objects (dust, dirt, ﬁngerprints, smudges, perspiration, saliva

etc.) on the camera window and/or the point source can greatly affect the quality of the captured

holograms. Take care not to touch the sapphire panes encasing the camera and the point source.

For further Care and Cleaning information, see Section 4.

The following is the list of parts of the HoloSea.

• HoloSea, pre-assembled. For full details, see the microscope description in Section 2.5.

–There are two purge valves on the bottom of the microscope, as shown in the right panel

of Figure 1

– DO NOT REMOVE THE PURGE VALVES. Using the purge valve without

the advice of 4Deep WILL NEGATE ANY WARRANTY on the microscope. Purging of

the microscope MAY only be needed in one speciﬁc issue, thus in most cases, your

microscope will NOT need to be purged.

2 INTRODUCTION HoloSea S7

Figure 1: The preassembled HoloSea Microscope (vertical view in left panel and horizontal view in

right panel).

• Long (10m) cable with 13 pin connector to power laser, camera, and transmit data from the

camera to the host computer.

Figure 2: 10m Cable

• Power supply (12 VDC)

HoloSea S7 2 INTRODUCTION

Figure 3: Power Supply (12 VDC)

• Carrying case

Figure 4: Carrying case, with the microscope inside

Please ensure all components listed above were shipped to you. If not, please contact

a 4Deep representative immediately.

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