AUTOMATE SCIENTIFIC ThermoClamp-3 Manuale utente
ThermoClamp®-3and -.
Temperature Controller
User’s Manual
Joe Cordes and David Barton
TM ©
®
©2021 AutoMate Scientic, Inc.
All rights reserved.
!This equipment must be earth grounded using the post on the
back of the controller.
ThermoClamp®-3and -.
Temperature Controller
User’s Manual
The ThermoClamp®is intended for research use only.
Table of Contents
Introduction..............................................................................3
Packing List
Features and specifications .................................................4
Electronic specications
Acronyms, abbreviations and definitions..........................5
Components.............................................................................6
Controller unit
Perfusion Pencil®
Perfusion Pencil Placement
Bath Perfusion / Water Jacket Feature
Chemical Information
Making sure the device works properly ...........................11
Getting Started.....................................................................12
Settings Menu........................................................................15
Monitoring the ThermoClamp from your computer......19
Autotuning..............................................................................24
TM ©
®
LED Display error codes.......................................................27
Updating firmware over the USB Port..............................28
Switching fluids......................................................................29
Operating without the bath sensor..................................30
Maintenance..........................................................................31
Cutting the Pencil tips .........................................................31
Max Temp LED .......................................................................31
Sensors ....................................................................................32
Replacement Parts................................................................33
Safety Instructions................................................................34
Warranty .................................................................................36
Table of Contents Continued
2
Introduction
FOR RESEARCH USE ONLY
The ThermoClamp is designed for solution-switching use in research
applications ONLY. AutoMate Scientic, Inc. cannot be responsible for
injury or death resulting from medical or pharmacological use.
Packing List
Item Quantity
ThermoClamp®-3 or -3.2
temperature controller 1
Heated Perfusion Pencil®manifold 1
Bath thermocouple 1
Power supply (12V DC) 1
This ThermoClamp user manual 1
3
Features and specifications
ThermoClamp-3 Controller
• Auto-tuning PID (Proportional Integral Derivative) temperature
control accurate to <1.0°C (often ±0.1˚C) with fuzzy logic
• Extremely low noise (analog heating) designed for electrophysiology
• Over-temperature protection
• Three types of user-congurable thermal runaway protection (patent
pending) with fuzzy logic constraints
• Smart voltage setpoint: digital, analog (BNC) or automatic
• Separate displays for both temperatures (control sensor and safety
sensor)
• Constant USB/serial data stream output
• Automatic heater failure detection and prevention
• Utilization of the safety sensor as secondary input for feedback control
Perfusion Pencil®Inline heater
• 1, 4, or 8 channels for solution heating with ow rates up to 5cc/min @
37˚C
• Temperature controlled bath perfusion inline heating with a ow rate
up to 5cc/min
• Small manifold volume for rapid switching
• Temperature can be maintained for the entire bath, or just for solutions
being delivered
• Perfusion Pencil tips are interchangeable / replaceable with standard
AutoMate tips
Electronic specifications
• Power supply: 100-240V AC, 50-60 Hz input; 12V, 5A output
• Heater output: 2 channels at quiet analog 0-12V up to 1A = 24 Watts
total
• Front panel BNC output voltage (to record the temperature): 40mV/
deg C
• Front panel input voltage (to control the setpoint): 20 deg C/V
• Serial baud rate: 230400
• Safety TC limit: 75˚C (adjustable)
• Max setpoint: 65˚C
• Default setpoint: 35˚C (adjustable)
4
Acronyms, abbreviations and definitions
CU ThermoClamp Controller unit
Isopotential Grounding (earth) point
PID Proportional Integral Derivative
TC Thermocouple (temperature sensor)
This equipment must be earth grounded using the post on the back of the
controller.
5
Components
Controller unit
The Controller Unit (CU) is shown in Figures 1 and 2. The integrated
temperature controller and power supply for the heater are the main
components of the controller unit.
Figure 1. Controller unit, front view
The sensor plugged-in to the “Control” port is used to control the
temperature. The thermocouple in “Safety” is just used to prevent
overheating of the Pencil. If you only use one sensor, connect it to "Safety."
Figure 2. Controller Unit, rear view.
NOTE: The ThermoClamp-3 and its heated Perfusion Pencil should both be
grounded for high-gain electrophysiology.
Pencil
connector
Two temperature
displays and
sensor ports
Power
switch
Power entry
module
USB port
Isopotential (grounding)
post
6
Perfusion Pencil®
The Heated Perfusion Pencil® (Pencil) manifold is shown in Figures 3,
4 and 5. The actual heating of the solutions happens in the Pencil. The
optional bath solution is also heated as it ows through the Pencil.
Figure 3. Side view of the Perfusion Pencil.
Figure 4. Detailed view of the rear part of the Perfusion Pencil.
Chamber
outow Thermal
insulation
Multiple solution
heater inow tubes
Power cables
to the CU
Drug ow orice.
Tighten removable tip
for minimum dead
volume
Chamber bath
inow
TC cable to CU
Multiple
solution heater
inow tubes
Pencil
mounting rod
Chamber bath
inow
Power cable to
the CU
7
Figure 5. Heated Perfusion Pencil schematic.
Perfusion Pencil Placement
Your Heated Perfusion Pencil must be placed as close as possible to the
perfusion chamber. If it is too far away, the liquid in your tubes will
lose all of its heat before it reaches the chamber. The maximum distance
between the Pencil and chamber depends on a number of variables
including: tubing material (thermal conduction), uid dripping versus
owing into the perfusion chamber via submerged tube, and ambient
temperature and drafts. You may be able to mount your Pencil farther
from the chamber if you try double-tubing – use a piece of large Tygon
tubing as thermal insulation outside a small piece of Teon tubing. Make
sure your solution ows into your chamber rather than dripping. The
extra exposure to cool room air will greatly decrease the delivered liquid
temperature. Finally, avoid placing your chamber in a location where
it is regularly blown by air drafts. Many researchers purchase or build
enclosures around their chambers or entire microscopes to help control the
temperature and avoid air drafts.
8
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