BD FACSFlow Supply System Manuale utente
23-11954-01
10/2020
For Research Use Only
Becton, Dickinson and Company
BD Biosciences
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San Jose, CA 95131 USA
bdbiosciences.com
BD Biosciences
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Tel +32.53.720.600
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BD FACSFlow™
Supply System
User’s Guide
For BD LSRFortessa™ Cytometers
Copyrights
© 2020, Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced,
transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in
any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without
prior written permission from BD Biosciences.
The information in this guide is subject to change without notice. BD Biosciences reserves the right to change
its products and services at any time to incorporate the latest technological developments. Although this guide
has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors
or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences
welcomes customer input on corrections and suggestions for improvement.
Trademarks
BD, the BD Logo, FACSClean, FACSFlow and LSRFortessa are trademarks of Becton, Dickinson and
Company or its affiliates. All other trademarks are the property of their respective owners. © 2020 BD. All
rights reserved.
Regulatory information
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
History
Revision Date Change made
23-11954-00 7/2013 Initial release for current BD LSRFortessa systems family.
23-11954-01 10/2020 Removed references of FACSRinse and added BD Detergent
Solution Concentrate in place of FACSRinse.
Contents
Chapter 1: About this guide 5
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2: System overview 9
Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Principles of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Sheath plenum enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Specifications and requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 3: Operation 19
Starting up the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Shutting down the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Responding to alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Chapter 4: Maintenance 25
Replacing the sheath cubitainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Replacing the waste cubitainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Flushing the BD LSRFortessa system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Flushing the BD LSRFortessa system with a BD HTS . . . . . . . . . . . . . . . . . . . . . 33
Replacing the air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Changing the sheath filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Sensors and tubing locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
1
About this guide
This guide describes the setup and operation of the BD FACSFlow supply system. The
BD FACSFlow Supply System User’s Guide is intended for laboratory personnel and
flow cytometry operators. Application-specific instructions are not included in this
guide.
This section includes these topics:
•Conventions (page 6)
•Technical support (page 7)
BD FACSFlow Supply System User’s Guide6
Conventions
Introduction The following table lists the safety symbols used in this guide to
alert you to potential hazards.
Safety symbols
Symbol Meaning
Caution alert
Identifies a hazard or unsafe practice that could result in
data loss, material damage, minor injury, severe injury, or
death
Biological hazard
Electrical hazard
Laser hazard
Fire hazard
Mechanical hazard, pinch points
Chapter 1: About this guide 7
Technical support
Introduction This topic describes how to get technical assistance.
Getting assistance
from this guide
For technical questions or assistance in solving a problem:
• Read the section of this guide specific to the operation you are
performing.
• See Troubleshooting (page 43)
Contacting
technical support
If additional assistance is required, contact your local
BD Biosciences technical support representative or supplier.
When contacting BD Biosciences, have the following information
available:
• Product name, part number, and serial number
• Any error messages
• Details of recent instrument performance
To contact customer support:
1. Go to bdbiosciences.com.
2. Select your region.
Information is displayed in your local language.
3. Click Go.
4. Click the Support link for contact details for your local region.
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BD FACSFlow Supply System User’s Guide10
Product description
Introduction The BD FACSFlow supply system is an automated sheath and
waste fluid control system designed for use with
BD LSRFortessa™ flow cytometers.
This topic describes the system and its components.
Product
classification
The BD FACSFlow supply system is For Research Use Only and is
not for use in diagnostic or therapeutic procedures. The system has
not been validated for clinical use.
System
components
The BD FACSFlow supply system houses 20-L sheath fluid and
waste cubitainers. Electronic cables and tubing connect the
BD FACSFlow supply system to the sheath plenum.
The photo shows the BD FACSFlow supply system and its
associated parts. The system includes:
• BD FACSFlow supply system unit
• Four-liter sheath plenum for the cytometer, stored in an
enclosure. See Sheath plenum enclosure (page 16)
Note: The sheath plenum is called the modified sheath
container in the BD FACSFlow Supply System Safety and
Limitations Guide.
• Probe sets and tubing with color-coded connectors
• Probe holders
Note: The system ships with a 10-L plastic container for use as a
waste container. You can use an empty sheath cubitainer for waste
as soon as one becomes available. This increases the capacity for
waste collection and requires fewer changes. Make sure to add
waste labels to the cubitainer to clearly indicate that it contains
waste and must be handled accordingly.
Indice
