Cairn MULTI SPLIT V2 Manuale utente
Quick Start
MULTISPLIT V2
Quick start guide July 2018
The MultiSplit is calibrated and QC’d prior to delivery, however some alignment is required upon initial
setup using the calibration cubes provided (containing a 50% mirror). A detailed setup guide is also
supplied; however, the following simplified instructions are useful for initial alignment.
2. Using brightfield illumination, focus on an image of a graticule and divert the microscope output to the camera.
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
1. Ensure all filter cubes are removed from the unit.
3. Close the adjustable aperture down to ensure one, small rectangle is present within the field of view. This single image is
only reflected by the internal MultiSplit mirrors and is therefore by default the longest wavelength channel once filter cubes are
in place. This image is transmitted through two dichroic mirrors and referred to as TT.
Image TT
No cubes in the unit
4. Locate the horizontal and vertical image separation controls at the front of the unit (labelled on the lid). When turned
anti-clockwise, TT should move toward the bottom right corner. If this is not the case, rotate your camera until this orientation
is achieved.
TT
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
5. Remove the magnetic door cover and insert the first calibration cube into the position labelled Horizontal Split - Cube 1,
which will generate your second image. This is reflected by the first filter cube, and transmitted by the second, hence the nomen-
clature RT.
TT Cube 1 inserted
6. Adjust the position of RT using the horizontal (H) and vertical (V) controls on the magnetic door cover until it reaches the
bottom left hand corner.
TTRT
7. Next insert Cube 2, which will cause image TR to appear.
TTRT Cube 2 inserted
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
8. Cube 2 is mounted internally on a carrier, with the adjustment controls mounted on the top plate. Adjust the position of TR
using the horizontal and vertical adjustment knobs associated with Cube 2.
Cube 1 inserted
Cube 2 inserted
TTRT
TR
9. Now insert the final cube into Cube 3 position on the back side panel of the unit, which will cause the final image, RR to
appear.
TTRT
TR
Cube 3 inserted
10. Cube 3 is housed in a carrier similar to Cube 2, with the H&V adjustments also on the lid to allow alignment of this image
in the top left corner.
Final adjustment of all controls can now be carried out for more precise image positioning.
TTRT
TRRR
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
When using long pass dichroic mirrors, the four images correspond to the following channels in order of descending wave-
length:
TT: (1) Longest channel
RT: (2)
TR: (3)
RR: (4) Shortest channel
For advice regarding dichroic mirrors and emission filter positions, please contact us with details of the four-way imaging you
with to achieve and we are happy to advise accordingly.
The MultiSplit has two auxiliary drop-in positions for each channel, accessible from the outer panels of the unit. Blank covers
are provided as standard; however, additional accessory kits can be provided with holders for a) Neutral Density filters or b)
Corrector lenses. Neutral Density filters are useful if one or more channels are disproportionately brighter than another’s. Cor-
rector lenses serve a dual purpose for chromatic correction, or to deliberately shift the focal plane for simultaneous multi-depth
imaging.
Our corrector lens kit provides a series of weak crown glass lenses and their optimal position for each channel is indicated be-
low. For precise focal plane shifting, we have a spreadsheet available to estimate the best focal length lens (if available). Please
contact us for this further detail and we will be happy to advise for your application.
MultiSplit Key Controls
Multi-depth Imaging
Cube 2 position
Cube 1 position
Corrector lens
position: TT
Corrector lens
position: TR
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
Horizontal and Vertical
Image Separation controls
Horizontal and Vertical controls
for Cube 2 and Cube 3
Cube 3 position
Corrector lens
position: RR
Corrector lens
position: RT
Indice
Altri manuali Cairn Convertitore multimediale
