Carton CN Series Manuale utente

CN Series Microscopes

Instruction Manual

COMPONENTS

14.

10.

11.

12.

13.

DESCRIPTIONS

1. Interpupillary Scale

Adjustable from 48mm through

75mm

2. Eyepieces

WF10x supplied as standard.

WF15x & WF20x are available

options.

3. Left eyepiece tubes with dioptic

adjustment ring

Ajustable for differences in eye

sight.

4. Right eyepiece tube

5. Right and Left Housings

6. Revolving Nosepiece

Inward-faced (reversed) quadruple

revolver accepting objectives

7. Objectives

4x,10x, 40x and 100x provided,

and 40x and 100x retractable and

sealed

Model CN-A

15.

16.

17.

18.

19.

20.

21.

22.

23.

24.

- 1 -

21. Mechanical stage control knob

Coaxial drive control on right

hand.

22. On-Off switch for illuminator

“I”=On, ”0”=Off

23. Base

With built-in 3 watts white LED

illumination powered by AC/DC

adapter.

24. Light intensity control dial

DESCRIPTIONS

8. Substage Condenser

With iris diaphragm and swing-out

filter holder

9. Swing-out Filter holder

For white diffusion filter provided

10. Locking screw for substage

condenser

11. Lever for iris diaphragm

12. Focusing knob for substage

condenser

13. Illuminator Condenser

14. Viewing head

Siedentopf type with 30°inclined

eyepiece tubes. Trinocular type also

available.

15. Locking thumb screw for head

Make sure to tighten it before use.

16. Stand arm

17. Double layers Mechanical Stage

Travelling through 70mm x 30mm.

18. Tension control ring

For coarse focusing adjustment

19. Coarse focusing adjustment knob

20. Fine focusing adjustment knob

- 2 -

SETTING UP YOUR MICROSCOPE

After opening the styroform container, check that you have the following parts and

accessories.

● Microscope stand complete

● Binocular or Trinocular Viewing head

● WF10x Eyepiece(s) (pair for Binocular or Trinocular head)

● Objectives in plastic container

● White diffusion filter

● AC/DC adapter 100-240VAC 50/60Hrz

● C-Wrench for tension control

● Immersion oil in container

● Dust cover

● Instruction manual

● For trinocular viewing head, C-mount tube and Photo tube

1. Place the stand on a level, stable surface. The microscope is easiest to use

when you face the front of the stage.

2. Loosen the head locking thumb screw provided on the side of the arm, and put

the viewing head onto the receptacle. The head can turn full 360°inside the re-

ceptacle. This permits observation from any direction. Tighten the thumb screw

to fix the head in any position you desire.

3. Put the eyepiece(s) into the eyepiece tube(s), being careful not to touch the

lenses.

4. Take out the objectives from the plastic containers and screw them into the re-

volving nosepiece from the lowest magnification to the higher one in clockwise

direction as you face the upside of the microscope.

5. Turn the switch off(O) and connect the AC/DC adapter to electric power sup-

ply.

C-mount tube

(for trinocular model only)

Photo tube

(for trinocular model only)

AC/DC adapter

Stand

Viewing head

Eyepieces

Objectives

C-wrench

- 3 -

USING AND ADJUSTING YOUR MICROSCOPE

Viewing Head:

The viewing head is inclined 30 degrees to enable comfortable observation and ro-

tates 360 degrees to permit observation from any direction. To rotate it, loosen the

head locking thumb screw and tighten it when the head is located at the position you

desire. Do not use the microscope without tightening the thumb screw. The optical

alignment is adjusted to be used with the thumb screw tightened.

The binocular and trinocular head, require interpupillary adjustment. To adjust the

eyepieces to the interpupillary distance between your eyes, please grasp the right &

left housings below the eyepiece tubes and move them farther apart or closer to-

gether in order to match your pupil distance. The correct adjustment is achieved

when your view becomes comfortable and presents a full single field. Read the in-

terpupillary scale and remember the distance of your pupil for your future use.

The dioptric adjustment ring is located on the left eyepiece tube. To correct a diop-

tric difference between your eyes, begin by looking through the right eyepiece with

your right eye, use the fine focusing handle to get the sharpest image of the speci-

men. Then, look through the left eyepiece with your left eye and turn the dioptric

adjustment ring until you get an image in the same sharpness as the right one. Make

the left eye adjustment without moving the focusing handle.

The CN series has Siedentopf type binocular or trinocular head is designed

to leave the mechanical tube length of 160mm unchanged when the interpupillary

distance is adjusted. This will not affect the diopter adjustment.

Arm:

The arm is fixed securely to the base and supports all the components above the

base. When moving the microscope, please grasp the arm and support the base with

the other hand. Do not carry or hold the microscope any other way.

Base:

Do not open the base. It contains various electrical components.

Illuminator:

Illumination is provided by a 3W LED light powered by AC/DC adapter and con-

denser lens. Turn the switch on (I) and rotate the light intensity control dial to adjust

the brightness.

- 4 -

Objectives:

DIN acrhomatic objectives are corrected for chromatic(colour) aberration. Your

microscopes may be equipped with Semi-plan or Plan achromatic objectives which

minimize the effects of curvature of field and produce flatter images. The 4 objec-

tives will be parfocal when properly fix to the nosepiece so it should not be neces-

sary to make large adjustments in focus when changing objectives.

Objectives 4x and 10x have relatively long working distances while the objectives

40x and 100x have a very short working distance. The 40x and 100x objectives are

retractable (spring-loaded in the lens mount) to protect the specimen slide.

Oil immersion objective 100x is made to get the correct magnification with the in-

termediate of immersion oil between the specimen slide and the objective. When

using 100x objective, begin focusing using the lower magnification objective. Put a

drop of oil on the illuminated area of the specimen and rotate the nosepiece to bring

the 100x objective into the optical path. Looking through the eyepiece, turn the fine

focusing handle to bring the specimen into focus. When bringing the objective into

position, pay attention to not introduce air bubbles into the oil as the bubble will

interfere with your observation. If an air bubble is introduced in to the oil it can eas-

ily be removed by turning the objective to the right and left slightly still touching the

oil on the slide. The air bubble will disappear. After using the 100x objective, be

sure to wipe off the oil thoroughly from the objective and the slide using a soft clean

cloth or lens tissue moistened with Xylol.

The objectives 40xR and 100xR are sealed to prevent damage from contact with

immersion oil, although the oil is not required for use with objective 40xR.

The high power objective requires bright light rays matching the Numerical Aper-

ture of the objective. The NA of the 100x objective is 1.25 and a condenser with

NA1.25 should be used with it. Numerical Aperture(NA) refers to resolving power

limits, and is expressed with formula NA = N x Sin U, N is refractive index of me-

dium (air or oil) between objective and slide, and U is half of the aperture angle.

Larger NA number relates to larger resolving power.

Eyepiece:

One pair of Wide Field eyepieces WF10x are supplied, having Field Number of 20

or 18. The FN20 eyepiece offers extra wide field of view and the high eye point of

18.8mm. The FN18 eyepiece has the eye point of 18.5mm, and a 19mm diameter

micrometer disc can be installed in it as option. The actual field of view of the mag-

nification is calculated as follows.

- 5 -

Field Number of eyepiece

Magnification of objective

So, the larger Field Number of eyepiece makes wider field of view for the objective.

WF15x with retainer for micrometer disc and WF20x without retainer are available

as option.

Mechanical Stage:

The long coaxial drive control mechanical stage is provided and makes the operation

much easier. It runs through 70mmx30mm.

The mechanical stage has vernier scales for measurement of movement in the X-Y

axis. The minimum reading is 0.1mm. By using the vernier you can record the po-

sition of the object on the slide by noting the readings on the scale. Repositioning

the slide to those readings makes locating the object very easy for future observa-

tions.

Focusing Device:

Coaxial coarse and fine focusing adjustment knobs are provided. Turning the coarse

focusing knobs raise the stage until it is stopped by the up stop. Then, looking

through the eyepiece, lower the stage by turning the coarse focusing knobs until you

find the object focused. To get sharper images, use the fine focusing knob. All the

objectives are made PAR-FOCAL. Once focusing is made with one objective, no re

-adjustment of focus by the coarse focusing knobs will be necessary when changing

to any other objective. To make the image sharpest, it may be necessary to make a

slight adjustment using the fine focusing knob.

The fine focus knob is graduated at both sides and each division corresponds to

0.004mm drive and one rotation of the fine focusing knob corresponds to 1.6mm

drive.

The tension control ring is located on the right side of

the coarse focus knob. The tightness of the coarse

focusing movement can be adjusted by turning this

ring using the C-Wrench. For instance, when you

change the sub stage condenser assembly to the phase

turret condenser, if you feel the need to adjust the ten-

sion of the coarse focusing movement, please use this

device. Tension control ring

with C-wrench

- 6 -

Substage Condenser:

Abbe type substage condenser (NA 1.25) focusable with rack and pinion mechanism

is provided. The top lens of the condenser is sealed to prevent damage from contact

with oil. The condenser is replaceable with phase turret condenser. The functions

of the condenser are to bring the light rays to a focus in the plane of the specimen

and to furnish a suitable cone of light to the objective. The diameter of the cone of

light is controlled by the iris diaphragm which provides a variable opening to fill the

back lens of the objective thereby utilizing the full resolving power of the objective.

When the objective 100xR is used, by using the

condenser focusing knob, always move the con-

denser to its highest position. The numerical

aperture 1.25 of the condenser corresponds with

the numerical aperture of the objective 100x.

To induce the full numerical aperture 1.25, it is

designed to be immersed with oil between the

top lens of condenser and the slide, without air

spacing correctly.

Care must be taken to wipe off the oil thoroughly from the condenser lens and the

slide immediately after each use. When the oil immersion objective is used, the con-

denser should always be raised to the highest position.

Iris Diaphragm:

The diaphragm is an important factor in obtaining good images. It is not intended to

control the brightness of the illumination. A large aperture transmits too much light

and creates an image with poor contrast. Smaller apertures increase contrast. To set

the proper aperture, make the aperture of the diaphragm largest and reduce until the

fine details of the specimen are imaged sharply.

Reducing the aperture increases contrast and depth of focus but it also reduces reso-

lution and introduces diffraction. The aperture must be selected for each objective:

i.e. the aperture for the 10x objective (NA 0.25) will not be the same as for the 40x

(NA 0.65), since the angle of light required is determined by the numerical aperture

of the objective. Proper adjustment of the diaphragm aperture is easily determined.

After placing an objective into the optical path and focusing, remove the eyepiece

and look through the optical tube. Observe the diaphragm through the objective,

then move the iris lever to coincide with the edge of the objective aperture.

- 7 -

MAINTENANCE OF MICROSCOPE

Model CN series microscope is designed such that only a minimum maintenance

work is necessary. Nevertheless proper care will extend the life of the instrument

considerably. There are five important rules you should observe.

1. Keep it away from dust. Always cover the microscope with the vinyl dust cover

provided, or put it back to the styrofoam container, whenever you are not using the

instrument. If dust gets on the lenses, blow it off with a rubber syringe or use a

camel's hair brush. Optical lenses are very soft, and can easily get scratched by

dust particles.

2. Do not touch the lens. Do not touch the lenses, finger print are difficult to remove.

Use a soft linen moistened with xylol to remove fingerprints.

3. Keep it away from moisture. Moisture is optical lenses worst enemy. Once you

get fungus on the lenses, they cannot be returned to their original condition. Keep

the instrument in a dry place.

4. Do not drop the microscope. Needless to mention, optical parts are very delicately

made and are easily broken. Also the mechanical parts are made with the highest

precision and will be damaged if dropped.

5. Do not dismantle. Fitting of all parts have been done with great care by skilled

experts, and once you dismantle, the smoothness of mechanical part or clearness of

optics will be spoiled. Return the instrument to an authorized dealer or to the

factory if need for dismantling arises.

Swing-out Filter Holder:

A filter holder is located under the iris diaphragm and a white diffusion filter is sup-

plied. Swing out the filter holder, insert the filter and swing it back to position.

The white diffusion filter diffuses and weakens the light. Other filters of 32mm dia.

can be used for different purposes if necessary.

- 8 -

PHASE CONTRAST SYSTEM

Phase contrast is a form of illumination which alters the normal path of light waves

from an object to a wavelength shifted diffraction pattern thus allowing visualization

of transparent or low contrast specimens. This allows living cells and suspensions to

be viewed without the need for staining procedures.

● Phase Set Components

● Phase Turret Condenser, N.A 1.25

● Achromat or Plan Phase Objectives (DM)

10X NA=0.25

20X NA=0.40

40X NA=0.65

100X (oil) NA=1.25

● Centering Telescope

● Green Filter

● Installation Key (Screwdriver)

● Storage Case

INSTALLATION AND ALIGNMENT

1. Replace the normal brightfield objectives of the microscope with the phase ob-

jectives (Dark & Medium type) supplied in the storage case.

2. Remove the brightfield substage condenser by loosening the condenser locking

thumb screw and gently lowering the condenser down and out of the mounting

ring. This thumb screw is located on the left side of the condenser mount as

you face the front of the microscope.

3. Install the Phase Turret Condenser by sliding it up into the condenser mount

and tightening the condenser locking thumb screw using the Installation Key

provided. The index mark (white dot) on the turret condenser should be situ-

ated at the front of the microscope.

4. Rotate the turret dial of the condenser to align the zero (0) mark to the index

mark on the turret housing. This is the setting to use for any brightfield work.

5. Raise the condenser to its maximum height and place a specimen slide on the

stage.

6. Swing the 10X objective into the optical path and focus.

7. Move the slide, using the mechanical stage, to a location with little or nothing

in the field of view.

8. Rotate the turret dial of the condenser to align the 10 mark to the index mark

(white dot) on the turret housing.

9. Replace either eyepiece with the Centering Telescope. Looking through the

telescope, you can see two rings of light, one at the center of view, the other

slightly off center.

- 9 -

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