DORIC OTPG 4 Manuale utente

Optogenetics TTL Pulse Generator

User Manual

Version 1.2.1

Contents

1 General Overview 3

2 Operations Guide 4

2.1 Channel Conﬁguration ............................................... 5

2.2 Control and Settings ................................................ 11

2.3 Acquisition View ................................................... 13

3 Speciﬁcations 14

4 Support 15

4.1 Maintenance ..................................................... 15

4.2 Warranty ....................................................... 15

4.3 Disposition ...................................................... 15

4.4 Contact us ...................................................... 15

General Overview

(a) 4-channel Generator (b) 8-channel Generator

Figure 1.1: Optogenetics TTL Pulse Generator.

The Doric Optogenetics TTL Pulse Generator (OTPG) allows the modulation of pulse signal trains as well as their trig-

gering. The generator connects to a computer using a USB-B cable, while each channel can be connected to a device

using a BNC cable. The most commonly used devices are LED Drivers,Laser Drivers and Shutters. It can be used to

coordinate between many input/output signal of diﬀerent devices.

The generator comes in 4-channel (Fig. 1.1a) and 8-channel (Fig. 1.1b) models. For the 4-channel OTPG, all channels

can either be used as an output, to deliver a pulse train, or as an input, to receive trigger signals. For the 8-channel

OPTG, a maximum of four of any ports can be used as inputs and all ports all can be used as outputs. The OTPG is

controlled using the Doric Neuroscience Studio (see chapter 2).

Operations Guide

The Optogenetics TTL Pulse Generator (OTPG; 4 or 8 channels) are controlled by the Doric Neuroscience Studio. Various

pulses train sequences can be designed for any type of experiment.

The OTPG user interface (Fig. 2.1) is split into two main sections: the Controls & Settings (Section 2.2) and the Acqui-

sition view (Section 2.3). From these sections, the Channel(s) conﬁguration (Section 2.1) window can be accessed to

add and conﬁgure channels.

Figure 2.1: Optogenetics TTL Pulse Generator Interface.

2.1 Channel Conﬁguration

2.1.1 Channel Conﬁguration Window Overview

Figure 2.2: Channels Conﬁguration Main Interface.

The Channels conﬁguration window is used to conﬁgure each channel. The window can be accessed by using either

the Add channel or Edit buttons. This window is separated into multiple sections shown in Figure 2.2 that are deﬁned

below.

1. The Channel Options section allows you to deﬁne the Channels Type and the Triggering Options. The diﬀerent

ﬁelds of this section are explained in more detail in section 2.1.2.

2. The Sequence Options deﬁnes the parameters of each pulse sequence for the channel. These parameters are

diﬀerent for each Channel Code. Should a parameter chosen be impossible to apply to a sequence (For example,

aTime ON greater than 1/Frequency), the color of the option boxes will turn RED. The diﬀerent ﬁelds for the

diﬀerent Channel Mode are explained in more detail in section 2.1.3

3. The Sequence Preview section shows a visualization of the output sequence that will be generated by the

current conﬁguration.

4. The Add button will save the current channel conﬁguration and enables a new channel to be conﬁgured. The

Close button will close the window without saving the current channel conﬁguration.

Chapter 2. Operations Guide 5

2.1.2 Channel Options Section

Figure 2.3: Channel Options of the Channel Conﬁguration Window.

The Channel Option section is separated in 2 sub-section, the OTPG Options section that deﬁnes the channel and its

mode and the Trigger Options section that control the trigger method of the selected channel. The Trigger Options

are not available when the channel is in Input mode.

OTPG Options

1. The Channel ﬁeld identiﬁes which of the available channels is currently being modiﬁed. The channel can be

changed by selecting a new one from the drop-down list.

2. The Mode ﬁeld identiﬁes the type of signal sent. Three modes are available, CW (Continuous Wave),Square,

and Input mode. Each mode enables diﬀerent options of the Sequence Option section that are explained in

more detail in section 2.1.3.

3. The Inverted Output checkbox reverse the signal output. When selected, the ON TTL signal will send 0 V, while

the OFF TTL signal will send 5 V.

Trigger Options

4. The Source trigger option allows to chose between a Manual Trigger (activated by the user) or an Input trigger,

which is coming from a channel input.

5. The Type deﬁnes the type of trigger that is used to start/stop a sequence. The Triggered type can starts and

stops a sequence at a rising edge while the Gated type can starts the sequence at a rising edge and stops it at a

falling edge. A more reﬁned interaction of the trigger with the deﬁned sequence can be set up using the Mode

ﬁeld. Not all Trigger Type are available for each combination of Trigger Mode and Repeatability. The diﬀerent

combinations are shown in Figure 2.8.

Chapter 2. Operations Guide 6

6. The Mode ﬁeld deﬁnes how the trigger activates a sequence. Each mode are not compatible with each combi-

nation of trigger type and repeatability. Figure 2.8 shows the diﬀerent available combinations for the diﬀerent

Trigger Modes. Four Modes are available and are the following:

•Uninterrupted: This mode activates the channel sequence when an input greater than 3.3 V is detected

by the BNC input. Following input pulses will be ignored while the sequence is running (Fig. 2.4). When

the Repeatable sequence checkbox is checked, the sequence will restart with the arrival of the ﬁrst input

pulse after the sequence has ﬁnished (Fig. 2.4b). This mode is available for Triggered pulse only.

Output Triggers

(a) Triggered Non-Repeatable Sequence

...

Output Triggers

(b) Triggered Repeatable Sequence

Figure 2.4: Uninterrupted Sequence Mode.

•Pause: This mode activates the channel sequence when a rising edge greater than 3.3 V is detected on

the BNC input (Fig. 2.5). Following input pulses (when Triggered, Fig. 2.5a) or falling edge (when Gated,

Fig. 2.5c) will pause the sequence and the sequence will continue when the next rising edge is received.

When the Repeatable sequence checkbox is checked, the sequence will restart with the arrival of the ﬁrst

input pulse after the sequence has ﬁnished (Figs. 2.5b and 2.5d).

Output Triggers

(a) Triggered Non-Repeatable Sequence

...

Output Triggers

(b) Triggered Repeatable Sequence

Output Triggers

...

Output Triggers

(d) Gated Repeatable Sequence

Figure 2.5: Pause Sequence Mode.

•Continue: This mode activates the channel sequence when a rising edge greater than 3.3 V is detected on

the BNC input (Fig. 2.6). The following input pulse (when Triggered, Fig. 2.6a) or a falling edge (when Gated,

Fig. 2.6c) will turn oﬀ the output, but the sequence will continue. The output will be turned back on at the

reception of the following rising edge. Triggers only aﬀect the output voltage value. When the Repeatable

sequence checkbox is checked, the sequence will restart with the arrival of the ﬁrst input pulse after the

sequence has ﬁnished (Figs. 2.6b and 2.6d).

•Restart: This mode activates the channel sequence when a rising edge higher than 3.3 V is detected on

the BNC input. The following input pulse (when Triggered, Fig. 2.7a) or falling edge (when i, Fig. 2.7b) will

Chapter 2. Operations Guide 7

Output Triggers

(a) Triggered Non-Repeatable Sequence

...

Output Triggers

(b) Triggered Repeatable Sequence

Output Triggers

...

Output Triggers

(d) Gated Repeatable Sequence

Figure 2.6: Continue Sequence Mode.

stop the sequence and the sequence will restart from the beginning when the next rising edge is received.

When the sequence is completed, it will restart with the next input pulse.

...

Output Triggers

(a) Triggered Non-Repeatable Sequence

...

Output Triggers

(b) Triggered Repeatable Sequence

Figure 2.7: Restart Sequence Mode.

Triggered Gated

Non-repeatable

sequence

Repeatable

sequence

Non-repeatable

sequence

Repeatable

sequence

Uninterrupted

Pause

Con�nue

Restart

Figure 2.8: Trigger options possibilities.

Chapter 2. Operations Guide 8

7. The Repeatable sequence checkbox, when selected, allows a sequence to be repeated. Not all modes and

trigger types can be repeated. Please refer to the Figure 2.8 to know the repeatable sequence combinations.

8. The Sequence Visualisation shows a graphical representation of the behavior of the selected Trigger Option

Type, Mode and Repeatability.

2.1.3 Sequence Options Section

CW (Continuous Wave) Mode

Figure 2.9: Sequence Options of the CW Channel Mode.

The CW (Continuous Wave) channel mode allows the creation of a continuous TTL signal. The following elements

appear in the Sequence Options section (Fig. 2.9).

1. The Starting Delay deﬁnes the time between the activation of the pulse sequence and the beginning of the

signal.

2. The Time ON deﬁnes the length of time the continuous signal is active. Should the time chosen be 0, the signal

will continue until the pulse is stopped manually.

3. The Total Duration shows the total expected duration of the pulse sequence. Should the duration be inﬁnite,

the box will display ∞. If there is an error in parameter selection, this box will display N/A.

Chapter 2. Operations Guide 9

Square Mode

Figure 2.10: Sequence Options of the Square Channel Mode.

The Square channel mode allows the creation of a square TTL pulse sequence. The Sequence Options of this mode

are shown in Figure 2.10 and are explained below.

1. The Starting Delay deﬁnes the time between the activation of the pulse sequence and the beginning of the

signal.

2. The Frequency sets the frequency (in Hz), which is the number of pulses per second. The frequency can also be

changed to the Period. For example, a signal at 10 Hz (frequency) will output one pulse every 100 ms (period),

whereas a signal at 0.5 Hz (frequency) will output one pulse every 2 seconds (period).

3. The Time ON deﬁnes the length of a single pulse. This time can also be converted to a Duty Cycle, which

represents the % of the period the pulse duration corresponds to.

4. The Pulse(s) per sequence set the number of pulses per sequence. If it is set to 0, the number of pulses will be

inﬁnite.

5. The Number of sequence(s) sets the number of times that the sequence will be repeated.

6. The Delay between sequences sets the delay between each sequence.

7. The Total Duration shows the total expected duration of the pulse sequence. Should the duration be inﬁnite,

the box will display ∞. If there is an error in parameter selection, this box will display N/A.

Input Mode

The Input mode (Fig 2.11) records a signal as long as there is a high TTL signal on the chosen console channel.

The channel can then be used as a trigger source for all the other channels of the OTPG. No Sequence Options or

Sequence Previews are available for this mode.

Chapter 2. Operations Guide 10

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