Edmund Optics TECHSPEC Manuale utente
STEP-BY-STEP INSTRUCTIONS FOR
BUILDING A FLUORESCENCE
MICROSCOPE
TECHSPEC®
Optical Cage System
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
INTRODUCTION 2
What is a Digital Fluorescence Microscope?
•Unlike traditional microscopes, which utilize an eyepiece for performing visual inspection,
digital video microscopes utilize cameras to record and capture images, providing higher
resolution and higher precision than what is typically seen with the human eye. Since
infinity-corrected digital video microscopes can be quite complex, using the modular
TECHSPEC Optical Cage System is ideal for simplifying the assembly. In this case
another aspect has been added to the design to incorporate a fluorescence aspect to the
digital microscope. Fluorescence microscopy is ideal for measuring and analyzing the
absorption and excitation of various wavelengths of light. An in-line fluorescence
microscopy setup utilizes a dichroic filter to redirect light from an illuminator into the
perpendicular optical path.
Why TECHSPEC Optical Cage System?
•Building a customized digital fluorescence microscope with TECHSPEC Optical Cage
components allows the user to build a microscope quickly and easily with limitless
customization options. Objectives can be changed to modify system magnification, and
interchangeable filter holders make swapping filters easy. Custom illumination can be
added for bright field, dark field, or in-line illumination options.Additionally, a
spectrometer could be added for precise spectral measurements.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OVERVIEW 3
Complete Parts List
Filter Selection
Sub Assemblies:
1. Dichroic
Assembly
2. Objective Arm
3. Source Arm
4. Imaging Arm
Mounting & Alignment
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
COMPLETE PARTS LIST 4
STOCK # Description QUANTITY
85713 25.4mm Dia. Adapter for 85710 1
85710 Square Tip/Tile Optic Mount 1
Depends on Application
25mm Dia. Dichroic Filter 1
85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1
85632 25mm Standard Cage Plate with M6 for Post Mounting 3
38944 0.316” ID, Fiber Optic Adapter SX-6 2
56371/56372
115V AC 50/60HZ, MH-100 Illuminator/220V AC 50/60HZ, MH-100
Illuminator 1
42346 1/4" x 24”, Flexible Fiber Optic Light Guide 1
85588 30mm Cage 25/25.4mm Diameter Thick Lens Mount 1
85737 30mm Square Interface Plate 5
85719 30mm Cage Plate C-Mount Adapter 3
55743 Mitutoyo to C-Mount 10mm Adapter 1
46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1
85488 Cage Support Rod 6mm Diameter x 100mm Length, M3 4
85486 Cage Support Rod 6mm Diameter x 50mm Length, M3 2
85490 Cage Support Rod 6mm Diameter X 150mm 4
85630 30mm Standard Cage Plate with M6(1 for Post Mounting) 3
86018 Filter Holder Plate 2
85649 Interchangeable Filter Holder 2
Depends on Application
Fluorescence Bandpass Filters (see details) 2
54774 MT-1 Accessory Tube Lens 1
58329 Mitutoyo MT-1/MT-2 C-Mount Adapter 1
85492 Cage Support Rod 6mm Diameter x 300mm Length, M3 4
54633 C-Mount Extension Tube (100mm Length) 1
54632 C-Mount Extension Tube (50mm Length) 1
03625 C-Mount Fine Thread Extension Tube (30mm-50mm Length) 1
65553 25mm Dia. 25mm Efl. Achromatic Lens 1
66014 25mm Dia. 20mm Efl. Aspheric Lens 1
59367 EO-3112C ½” CMOS Color USB Camera 1
59382 5m USB2.0 (mini-B to standard-A) Cable 1
85618 Micrometer Mount Kit 1
58661 Micrometer Head Anvil 13mm 1
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OPTICAL FILTERS 5
A basic fluorescence microscope requires 3
filters. Each application will use a different
combination depending on the system
requirements:
•Excitation Filter: placed within the
illumination path of a fluorescence
microscope. It filters out all wavelengths of the
light source except for the excitation range of
the fluorophore or specimen under inspection.
•Dichroic Filter: placed between the excitation
filter and emission filter at a 45° angle. It
reflects the excitation signal towards the
fluorophore under inspection and transmits the
emission signal towards the detector.
•Emission Filter: placed within the imaging
path of a fluorescence microscope. It filters out
the entire excitation range of the fluorophore
under inspection and transmits the emission
range of the fluorophore.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
1. DICHROIC ASSEMBLY
6
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
DICHROIC ASSEMBLY 7
STOCK # Description QUANTITY
85713 25.4mm Dia. Adapter for 85710 1
85710 Square Tip/Tile Optic Mount 1
N/A 25mm Dia. Dichroic of choice (see details under filter) 1
85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1
85632 25mm Standard Cage Plate with M6 for Post Mounting 1
85737 30mm Square Interface Plate 1
86018 Filter Holder Plate 2
Parts needed for this step:
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
DICHROIC ASSEMBLY 8
1. Carefully insert the 25mm Dia. Dichroic
Filter into the 25.4mm Dia. Adapter (#85-
713). Lock into place with the M3 set
screws. Insert the adapter into the Square
Tip/Tilt Optic Mount (#85-710) and secure.
2. Attach the 25mm Standard Cage Plate
(#85-632) to the Cage Sphere (#85-624)
opposite the 43mm port with M3 SHCS.
Slide the base of the tip/tilt assembly from
step 1 into the sphere and secure within the
plate. (Alignment will be done later).
3. Attach two Filter Holder Plates (#86-018)
with M3 SHCS to two adjacent sides of the
sphere. Keep the the openings of the plates
facing the 43mm port. Attach a Square
Mounting Plate (#85-737) to a side opposite
of a filter holder plate.
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
2. OBJECTIVE ARM
9
PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
OBJECTIVE ARM 10
STOCK # Description QUANTITY
85737 30mm Square Interface Plate 2
55743 Mitutoyo to C-Mount 10mm Adapter 1
46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1
85719 30mm Cage Plate C-mount Adapter 1
85490 Cage Support Rod 6mm Diameter X 150mm 4
85488 Cage Support Rod 6mm Diameter x 100mm Length 2
85-618 Micrometer Mount Kit 1
58-661 Micrometer Head Anvil 13mm 1
Parts needed for this step:
Indice
