Genfine Purifier 32 Manuale utente

GENFINE

Purifier 32

User Manual V1.3

GENFINE Biotech (Changzhou) Co., Ltd.

 
 
 
 
Contents  
Chapter 1: Product Introduction ...............................................................................................................1
Product Name...................................................................................................................................1
Model ...............................................................................................................................................1
Intended Use ....................................................................................................................................1
Working Principle ............................................................................................................................1
Chapter 2 Function Introduction...............................................................................................................2
Main .................................................................................................................................................2
Magnetic Bead Processing Principle................................................................................................4
Magnetic bead processing ................................................................................................................4
Bind magnetic beads ........................................................................................................................4
Release magnetic beads....................................................................................................................4
Rinse magnetic beads.......................................................................................................................5
Volume change in magnetic bead processing...................................................................................5
USB Port ..........................................................................................................................................5
Consumables ....................................................................................................................................5
Chapter 3 Installation and Packaging .......................................................................................................6
Delivery Check ................................................................................................................................6
Unboxing..........................................................................................................................................6
Integrity and Damage Check............................................................................................................6
Environment Requirement ...............................................................................................................7
Precautions and Restrictions ............................................................................................................7
Installation Settings ..........................................................................................................................8
Power Connection ..........................................................................................................................10 
Packaging Settings .........................................................................................................................10 
Chapter 4 Instructions for Use................................................................................................................12 
Starting...........................................................................................................................................12 
Control Panel..................................................................................................................................12 
Touch Screen..................................................................................................................................12 
System Default ...............................................................................................................................12 
Select File.......................................................................................................................................13 
Run File..........................................................................................................................................13 
Custom ...........................................................................................................................................14 
Sign Up ..........................................................................................................................................14 
Log in .............................................................................................................................................15 
New Program Creation...................................................................................................................16 
Program Details..............................................................................................................................16 
Function Details .............................................................................................................................18 
Settings...........................................................................................................................................20 
Advanced Mode .............................................................................................................................20 
Shutting Down ...............................................................................................................................21 
Emergencies ...................................................................................................................................22 
Chapter 5 Care and Maintenance............................................................................................................23 
Platform Cleaning ..........................................................................................................................24 

 
 
 
Magnetic Rods Cleaning ................................................................................................................24 
Waste Disposal ...............................................................................................................................24 
Decontamination Procedure ...........................................................................................................25 
Packaging for repair .......................................................................................................................26 
Service Contract .............................................................................................................................27 
Disposal of the instrument .............................................................................................................27 
Chapter 6 Technical Parameters .............................................................................................................29 
General Parameters ........................................................................................................................29 
Performance parameters.................................................................................................................29 
Safety parameters...........................................................................................................................30 
Compliance ....................................................................................................................................30 
Chapter 7 Other information...................................................................................................................31 
Production date ..............................................................................................................................31 
Service life .....................................................................................................................................31 
Components List ............................................................................................................................31 
Interpretation of Graphics, Symbols and Abbreviations ................................................................31 
Manual revision date ......................................................................................................................32 
2021.01.29......................................................................................................................................32 
Target customers ............................................................................................................................32 
More information ...........................................................................................................................32 
Safety and User Guide ...................................................................................................................32 
Chapter 8    Manufacturer and Medical Device Information..................................................................34 
Name of manufacturer....................................................................................................................34 
Production address .........................................................................................................................34 
Contact information .......................................................................................................................34 
Production record certificate No. ...................................................................................................34 
Medical device record certificate/technical requirement No..........................................................34 
Chapter 9 Order Information ..................................................................................................................35 
Purifier 32 ......................................................................................................................................35 
List of accessories and consumables ..............................................................................................35 
List of kits ......................................................................................................................................36 
Appendix A Certificate of Decontamination ..........................................................................................37 
Appendix B System Log ........................................................................................................................38 
 
              
 
 
 
 
 
 
 

Page1of 38

Chapter 1:

Product Introduction

Product

Name

Automatic nucleic acid extractor

Model

Purifier 32

Intended Use

This instrument can be applied for the extraction and purification of

nucleic acid from different clinic samples. Purifier 32 is especially

used on research and tests by the personnel who have been trained

professionally. It is recommended that good laboratory practice

(GLP) should be followed to ensure the reliability of the analysis.

Refer to Chapter 6: Technical Parameters.

Working

Principle

The principle is magnetic beads adsorption method. Purifier 32

(Figure 1-1) is designed for automatic transfer and processing of

magnetic beads in standard microwell plates. The patented

technology of the Purifier 32 system is based on the use of

magnetic rods and microwell plates with disposable and specially

designed magnetic tip combs. The instrument may work without

any distribution or suction parts or devices. Samples and reagents,

including magnetic beads, are loaded into microwell plates

according to corresponding instructions. The program selected by

the user through the touch screen has been uploaded to the

instrument software. Purifier 32 software can be used to create and

run programs.

Figure 1-1. 32-Channel

Automatic Extractor

Page 2 of 38

Chapter 2

Function Introduction

Main

Components

It usually consists of a mechanical part and an electrical part. This

section shows the front and back views of Purifier 32.

Front View

See Figure 2-1 for the front view of Purifier 32.

Figure 2-1. Purifier 32 Front View

96 deep-well plate &

loading position

Magnetic tip comb holder

Magnetic rod

USB port

Sample chamber door

Touch screen

Magnetic tip comb

Page 3 of 38

Rear View

See Figure 2-2 for the rear view of Purifier 32.

Figure 2-2. Purifier 32 Rear View

Purifier 32 (Figure 2-1) has 2 microwell plate positions. The

magnetic tip combs match the microwell plate. During each step, the

microwell plate remains stationary and the only component that

moves is the magnetic head with magnetic tip comb and magnetic

rod. The magnetic head consists of two vertically moving platforms.

One is used for the magnetic rods and the other is used for magnetic

tip comb. The magnetic tip comb with 32 channels can process 32

samples at one time.

Prior to the start of program, samples and reagents are loaded into

microwell plate. The magnetic tip comb is placed on the correct

microwell plate and can be automatically loaded onto the moving

platform. According to the program, the microwell plate is placed in

the corresponding plate position on the plate.

The sample chamber door must be closed during operation. It can

protect the sample from environmental contaminants.

The working principle of the instrument is MPP technology (Figure

2-4). Different from other methods of magnetic bead purification for

liquids transfer, this method transfers magnetic beads between

microwell plates containing specific reagents. The magnetic beds are

transferred with the help of magnetic rods and the magnetic rods are

loaded with a specially designed disposable plastic magnetic tip

comb.

Filter

Fan

Label

Main

power

connection

Page 4 of 38

Magnetic

Bead

Processing

Principle

Figure 2- 3 MPP

Magnetic

bead

processing

Magnetic bead processing can be divided into four separate steps:

⚫Bind magnetic beads

⚫Release magnetic beads

⚫Rinse magnetic beads

⚫Concentration

Bind

magnetic

beads

In the magnetic bead binding process, the magnetic rod is

completely inside the magnetic tip comb. The magnetic rod and

magnetic tip comb move slowly up and down in the microwell plate

to collect the magnetic beads to the top of the magnetic tip comb.

After the magnetic rod and magnetic tip comb are bound with the

magnetic beads, they are lifted from the microwell plate and

transferred to the next microwell plate.

Release

magnetic

beads

After the magnetic beads are collected, the magnetic rod and

magnetic tip comb are lifted from the microwell plate together and

transferred to the next microwell plate containing the reagent. The

magnetic rod and magnetic tip comb are placed together and the

magnetic rod is lifted.

The magnetic beads are released by moving the magnetic tip comb

up and down at high speed, and all the magnetic beads are fully

mixed with the substrate of the next reaction.

Page 5 of 38

Rinse

magnetic

beads

Magnetic bead cleaning is a common and important process that

combines release and collection in a microwell plate filled with

cleaning solution.

In order to maximize cleaning efficiency, the magnetic rod and

magnetic tip comb are designed to have minimal liquid hanging

performance.

Volume

change in

magnetic

bead

processing

The solution volume of the former microwell plate can be larger

than that of the next microwell plate to achieve the purpose of

concentration (see Figure 2-5).

Figure 2-4 Concentration Process in Magnetic Bead Processing

USB Port

There is a USB port on the front panel for updating the software of

the touch screen and motherboard.

Consumables

See "Accessory and Consumables List" for details and ordering

information about Purifier 32's plastic consumables, such as

deep-well plates, magnetic tip comb, HEPA filters and UV lights.

Page 6 of 38

Chapter 3

Installation and Packaging

This chapter introduces the installation of Purifier 32

Delivery

Check

This section contains the procedures to be carried out upon receipt

of the instrument.

Unboxing

Move the packaged instrument to the operation site. To prevent

condensation of water vapor, the instrument should be left in its

protective, anti-static plastic packaging until the ambient

temperature is reached. Open Purifier 32 and accessories carefully,

with the arrow on the transport package upwards. Remove the

instrument from the package and place it on a horizontal surface.

Warning Purifier 32 weights about 22kg. If transporting without

package, please handle carefully. It is recommended that two

people move the equipment together and take appropriate

precautions to avoid injury.

When lifting the instrument, place the fingers on the bottom of both

sides and keep the back straight.

Please keep the original packing materials for future

transportation. The packing is designed to ensure safe

transportation and reduce damage in transit. The use of alternative

packaging materials may not achieve the effect. All instrument

related documentation provided by the manufacturer is retained for

future use.

Integrity and

Damage

Check

Check the packing list attached to the instrument according to the

order. Visually check the shipping packages, instruments and

accessories for possible transportation damage. If any parts are broken,

please contact the manufacturer in time.

Page 7 of 38

Environment

Requirement

When installing Purifier 32, avoid placing it in the places where

there is a lot of dust, vibration, strong magnetic field, direct sunlight

or ultraviolet radiation, airflow, high humidity or high temperature

fluctuation. Please place the instrument on a regular experiment

table to ensure that:

⚫The working area is flat, dry, clean and secure, with extra

space for accessories, cables, reagent bottles, etc;

⚫At least 10 cm of free space is provided for ventilation on the

table around the instrument;

⚫The ambient air is clean, free from corrosive steam, smoke

and dust;

⚫Ambient temperatures range from 10°C to 40°C;

⚫Humidity is low and condensation does not occur (relative

humidity is below 80%);

Care should be taken not to operate the instrument in an

environment with potentially harmful liquids or gases.

Precautions

and

Restrictions

⚫Always ensure that the laboratory power supply voltage meets

the specification on the label on the back of the instrument

(Figure 2-2);

⚫Do not smoke or eat or drink while using this device;

⚫Clean hands thoroughly after handling the buffer solution;

⚫Follow regular laboratory procedures for handling samples

that may be dangerous;

⚫Adhere to good laboratory practices and wear appropriate

protective clothing such as disposable gloves and laboratory

coat;

⚫Ensure that the working area is well ventilated;

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