Labomed TCM 400 FLR Manuale utente

To ensure proper use of this instrument as well as to avoid injury while operating

instrument, understanding this manual completely before use is highly recommended.

User Manual

TCM 400FLR

Inverted Microscopy

7127000-795

________________________________________________________________________________________________________

Issue 1.1

Printed on Aug. 2018

TCM400Flr

CONTENTS

9 TROUBLESHOOTING 8

10 TECHNICAL SPECIFICATION 9

8 OBSERVING A SPECIMEN 7

7 CENTERING THE LIGHT BEAM 7

6 MAINTAINING OPERATING LIFE 6

5 INSTALLATION 4

4 SAFETY INSTRUCTIONS 3

3 UNPACKING 2

2 PRINCIPLE 1

1 INTRODUCTION 1

PACKAGING CONTENTS

1. FL attachment in 2 parts

2. 2 Filter cube

3. Power Supply

4. Power cord

5. UV protection shield

FluorescenceAttachment User Guide 2

SAFETY INSTRUCTIONS

Fluorescent attachment is manufactured according to CE safety norms and regulations.

Fluorescent attachment is intended for use only as prescribed in this manual.

Make sure the main switch on the power supply unit is set to OFF (O) before supplying power to the unit.

The power supply unit contains high voltage components. Do not attempt to disassemble the unit when turned ON

(I).

To avoid injury, do not remove the lamp housing while the lamp is lit. Also, do not turn the lamp ON while the lamp

housing is removed to avoid damaging the instrument.

Before opening lamp housing for replacement of the lamp, set the main switch to OFF (O) and unplug the lamp

housing's connecting cord plug from the output connector on the power supply unit. Wait at least 10 minutes or until

the lamp housing cools down before any changes are made.

Do not open the lamp housing while the system is turned ON, for any user contact with internal parts may cause

injury.

A used mercury burner should be disposed of in compliance with the ordinances or regulations of your national or

local government.

The manufacturer will not accept any liability for damage caused by any user following unsafe practice of the

instrument.

FluorescenceAttachment User Guide 3

Avoid using the lamp beyond its service life. The mercury lamp seals high pressure gasses. If the lamp is used beyond its service life,

stress may accumulate inside the lamp and in the worst but very rare case, the lamp may burst.

INSTALLATION

Remove all parts from the packing carefully.

1. Remove the plastic cover (1) from the microscope to make room for

the ﬂuorescence attachment.

Bring the nosepiece to the maximum height by rotating the coarse

knob (2) anti- clockwise. Remove the illumination power cable before

removing the cover. Apply similar pressure by thumbs on the inner

sides of the panel (3 & 4) to push it out. Refer to ﬁgure 1 and 2.

2. Join the Optical assembly and illumination assembly to complete the

FL attachment. Guide the illumination assembly to the optical

assembly. Secure both parts by the allen screws (1) provided. Refer

Fig. 3.

3. Assemble attachment in the microscope as shown in Fig. 4 and

secure it with 3 Nos of Screws. Then install filter in the guide slot as

shown in Fig. 5.

FluorescenceAttachment User Guide 4

Fig. 4

Fig. 3

Fig. 5

Fig. 1

Fig. 2

INSTALLATION

Open the illumination house to install the mercury lamp. Loosen the

thumbscrew indicated by the arrow and gently pull out lamp door from lamp

housing.

To avoid any damage to the mercury vapor lamp, a plastic rod (1) is ﬁtted in

place of lamp during transit. Remove this rod by loosening the two screws

indicated by the two arrows A and B shown in Fig. 6.

Mount the mercury vapor lamp in place of plastic rod and tighten the screws

to secure the lamp. install as shown in Fig. 6.A.

Avoid touching the lamp in order to prevent leaving ﬁnger prints on the

glass. If there is any residue on the lamp's surface, gently wipe it off with

soft tissue paper.

Install the power cable coming from the illumination house to the power unit

indicated by arrow A in Fig. 7.

(1). Connect the power cord to theAC Inlet indicated by arrow B in Fig. 7.

FluorescenceAttachment User Guide 5

Fig. 7

Fig. 6

B A

Fig. 6A

INSTALLING THE FILTER B & G

Select the ﬁlter to be used on the ﬂuorescence attachment.

Engage the slider to the ﬁlter house and gently slide it in.

It will stop at the Iindex position.

Ensure that the power supply unit has been connected to proper power supply voltage.

Set the main switch on the power supply unit to ( | ). After the lamp is ignited, at least 5 minutes required to

stabilizelight..

To avoid shortening the power supply unit's operating life, avoid turning power ON while the lamp is not mounted

Avoid turning power ON and OFF within short intervals for this may reduce working life of the power supply.

After the lamp is turned OFF, it should not be re-ignited before the mercury vapors cool and condense. Wait

approximately 10 minutes before turning power to the ﬂuorescent attachment O N again.

MAINTAINING OPERATING LIFE

FluorescenceAttachment User Guide 6

Fig. 8

CENTERING THE LIGHT BEAM

First allow the mercury vapor lamp at least ﬁve

minutes to fully illuminate for optimal use (it is very

important to allow the system enough time to fully

ignite in order to achieve a fully illuminated centering

beam). Look into the eyeppiece aanddmanipulate

the ccollector focusing knobb to make the field asd

bright as regular asapossible, then, turn the knobb

clockwise to tighten as shown in Fig.#9/Part 1.

When the lamp is fully illuminated, a beam of light is

visible through the eyepieces in the ﬁeld of view.

To center this beam of light in the ﬁeld of view, two

centering knobs are located on the lamp housing as

indicated by the two arrows (A & B) in Fig. 9. By

adjusting these two knobs, the focused beam will

shift. Adjust the beam position to the center of the

ﬁeld of view as shown in Fig. 9. A.

OBSERVING A SPECIMEN

FluorescenceAttachment User Guide 7

Fig. 9

Turn the power supply ON. Allow the lamp at least 5 minutes to

fully illuminate for optimal use.

The halogen illumination of the microscope should be kept off

while working with FLR.

Place the specimen slide on stage. Select the excitation light by

installing the ﬁlter block as follows :

For G-excitation - Install red sliding block to its outermost position.

For B-excitation - Push blue sliding block in to its innermost position.

For O light (non-excited light) - Slide out the ﬁler to parking point.

Observe the specimen slide through the microscope's eyepieces.

When no ﬂuorescent light is required, turn the attachment's power

supply OFF and set the sliding ﬁlter block to its middle / neutral

position. To view the specimen in normal condition under the

microscope's light, turn the microscope ON.

Fig. 9A.

TROUBLESHOOTING

FluorescenceAttachment User Guide 8

Problem Cause Remedy

Optical System

1) The mercury lamp lights up, Filter slider is not engaged properly Adjust ﬁlter to required setting.

but ﬁeld of view remains dark in light path.

2) Image is unclear, blurred or a) Objectives and/or ﬁlters are dirty. Clean them with tissue/lens paper.

has poor contrast. b) Field iris diaphragm is not Adjust ﬁeld iris diaphragm so

fully opened. that the image circumscribes

the ﬁeld of view.

3) Field of view is vignetted or a) Objective is not engaged properly Be sure when rotating nosepiece

it is not evenly illuminated. in light path. that objective clicks into position.

b) Filter slider is not engaged properly Engage required ﬁlter

in light path. properly in light path.

c) Mercury lamp is not centered Center mercury lamp correctly.

properly. This process is shown on Page 7

d) Beam focus deviates from correct Adjust the condenser knob.

position. This process is shown on Page 7

4) Mercury lamp is not illuminating Some mercury lamps may not ignite Press the Trigger button provided

after switching power supply ON. the ﬁrst time the power supply is on the front panel of power supply

turned ON. unit and wait for lamp to ignite.

Electrical System

1) Lamp housing / Main switch a) Power cord is not connected Make sure power cord is ﬁrmly

indicator does not light up. properly. in place.

b) The fuse is blown. Replace with fuse of same type

and rating.

2) Main switch indicator lights up, a) Connector cable is not engaged Verify connection is secure.

yet mercury lamp remains OFF. properly.

b) Mercury lamp is not mounted. Install mercury lamp as

indicated on Page 5

3) Light demonstrates partial a) Sufﬁcient time has not been Allow at least 5 minutes after

ﬂickering. allowed for the lamp to fully turning power supply ON for the

illuminate. lamp to fully illuminate. If ﬂickering

persist press Trigger button.

b) Lamp's service life has expired. Replace the mercury lamp

Indice

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