Leica DM2000 Manuale utente
Leica DM2000
Leica DM2500
Operating manual
Bedienungsanleitung
Mode d’emploi
MICROSYSTEMS
Phone/Tel./Tél. +49 (0) 64 41-29 22 86
Fax +49 (0) 64 41-29 22 55
E-mail/Email/Courriel MQM-Hotline@leica-
microsystems.com
Published September 2004 by/
Herausgegeben September 2004 von/
Publiée en septembre 2004 par :
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Strasse
D-35578 Wetzlar (Germany)
Responsible for contents/
Verantwortlich für den Inhalt/
Responsable du contenu rédactionnel/
Dr. Jasna Röth, Peter Schmitt
(Clinical Microscopy, Product Management)
Holger Grasse
(Safety Officer according to MPG §30/
Sicherheitsbeauftragter nach MPG §30/
Responsable de la sécurité conformément à la loi
relative aux dispositifs médicaux, §30)
In case of questions, please contact the hotline/
Bei Fragen wenden Sie sich bitte an die Hotline/
Pour toute question, contacter notre service
d’assistance téléphonique :
3
Leica DM2000
Leica DM2500
Operating Manual
4
Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems Wetzlar GmbH. Reproduc-
tion of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in-
cluding electronic systems) is not allowed with-
out express written permission from Leica
Microsystems Wetzlar GmbH.
The term "Windows" can be used in the follow-
ing text without further identification. It is a reg-
istered trademark of the Microsoft Corporation.
Otherwise, no inference with regard to the free
usability of product names may be drawn from
the use of those names.
The instructions contained in the following
documentation reflect state-of-the-art technol-
ogy and knowledge standards. We have com-
piled the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manu-
al’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.
5
Contents
8.4 Tubes ........................................................... 43
8.5 Eyepieces.................................................... 44
8.6 Objectives ................................................... 45
8.7 Light Sources ............................................. 46
8.8 Aperture Diaphragm ................................. 47
8.9 Field Diaphragm ......................................... 48
9. Contrasting Methods ................................ 49
9.1 Transmitted Light....................................... 49
9.1.1 Brightfield......................................... 50
9.1.2 Phase Contrast................................ 51
9.1.3 Darkfield ........................................... 51
9.1.4 Oblique Illumination ....................... 52
9.1.5 Polarization ...................................... 52
9.1.6 Differential
Interference Contrast .................... 53
9.2 Fluorescence.............................................. 55
10. Measurements with the Microscope ... 56
10.1 Linear Measurements .............................. 56
10.2 Thickness Measurements ....................... 57
10.3 Differentiation of Gout / Pseudo Gout ... 58
11. Troubleshooting ........................................ 60
12. Care of the Microscope ........................... 64
12.1 Dust Cover .................................................. 64
12.2 Cleaning....................................................... 64
12.3 Handling Acids and Bases ...................... 65
12.4 Changing Fuses.......................................... 65
13. Essential Wear and Spare Parts............ 66
14. Retrofitting Components.......................... 67
14.1 Fitting the Filter Magazine
(Transmitted Light) .................................... 67
14.2 Equipping the Condenser Disc................ 67
15. Index ............................................................ 70
16. EU Declaration of Conformity................. 71
Contents
1. Important Notes about this Manual ...... 6
2. Intended Purpose of Microscopes ........ 7
3. Safety Notes ............................................... 8
3.1 General Safety Notes ............................... 8
3.2 Electrical Safety ........................................ 8
4. Overview of the Instrument .................... 10
5. Unpacking the Microscope .................... 15
6. Assembling the Microscope .................. 17
6.1 Stage ............................................................ 17
6.2 Condenser................................................... 19
6.3 Tube and Eyepieces.................................. 20
6.4 Objectives ................................................... 20
6.5 Light Source - Transmitted Light Axis ... 20
6.6 Components for Fluorescence
Applications................................................ 23
6.6.1 Fluorescence Illuminator .............. 23
6.6.2 106 z Lamp Housing........................ 23
6.6.3 Equipping the
Fluorescence Turret Disk.............. 26
6.7 Analyzer and Polarizer ............................ 27
6.8 Lambda Plate Compensator .................... 28
6.9 DIC Prisms .................................................. 28
6.10 Optional Accessories ............................... 28
6.11 Connection to the Power Supply............ 30
7. Start-up........................................................ 31
7.1 Switching on the Microscope................. 31
7.2 Köhler Illumination .................................... 31
7.3 Checking Phase Contrast Rings ............. 33
7.4 Adjustment of Condenser Prisms........... 34
7.5 Adjusting the Light Sources .................... 36
8. Operation .................................................... 40
8.1 Switching on............................................... 40
8.2 Stages and Object Displacement........... 40
8.3 Focusing ...................................................... 41
6
1. Important Notes about this Manual
(1.2)
→
p.20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle sym-
bol shown here, and have a gray back-
ground.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Explanatory note.
Item not contained in all configurations.
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is as-
sembled, put into operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
7
2. Intended Purpose of Microscopes
2. Intended Purpose of Microscopes
The Leica DM2000 and DM2500 microscopes, to
which this user manual belongs, are designed
for biological routine and research applications.
This includes the examination of samples taken
from the human body with a view to provide in-
formation on physiological or pathological
states or congenital abnormalities, or to deter-
mine the safety and compatibility with potential re-
cipients, or to monito therapeutic measures.
All the above-named microscopes comply with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. They also conform to the
Council Directives 73/23/EEC concerning electri-
cal apparatus and 89/336/EEC concerning elec-
tromagnetic compatibility for use in an industrial
environment.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems Wetzlar GmbH.
In such cases the conformity declaration
shall cease to be valid.
Caution!
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.
8
3. Safety Notes
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar, Germany must be consulted
whenever the device is altered, modified or
used in conjunction with non-Leica compo-
nents that are outside of the scope of this
manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!
3. Safety Notes
3.2 Electrical Safety
General Specifications
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
DM2000
DM2500
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
90-250 V~
50-60 Hz
90 W
160 W
F 3,15 A 250 V
15-35°C
max. 80% to 30°C
II
2
9
3. Safety Notes
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
Caution!
Before exchanging the fuses or lamps, be
absolutely certain to switch off the main
power switch and remove the power cable.
Caution!
Through activating to the grounding connec-
tion (earth screw at the back of the stand)
ancillary equipment with its own and/or ex-
tra power supply may be brought to the
same ground wire potential. For connections
without a ground connector, Leica Service
must be consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using patched fuses or
bridging the fuse holder is not permitted.
The use of incorrect fuses may result in a
fire hazard.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water.
Water can cause electric shock.
10
4. Overview of the Instrument
Specification
Contrast Method
Transmitted Light Axis
Incident Light Axis
Tube
Magnification Changer
(optional)
Objective Turret
X/Y Stage
Leica DM2000
Integrated halogen illumination
manual adjustment of
•Light intensity
•Aperture diaphragm
•Field diaphragm
Leica DM2500
Lamp Housing
Manual adjustment of
•Light intensity
•Aperture diaphragm
•Field diaphragm
•Transmitted Light: Brightfield, Darkfield, Phase Contrast,
Polarization, Differential Interference Contrast
•Incident Light: Fluorescence
Incident-light fluorescence illuminator for up to eyepiece field
number 22 with
•5-filter turret disk
•Centerable aperture and field diaphragm
•Light trap for the suppression of extraneous light
•N4 neutral filter and shutter, switchable
optionally with
•Fixed or variable viewing angle
•Up to 3 switching positions
•One or two camera ports
•Ergotube with height-adjustable eye level and camera port
•Manual
•Magnification steps: 1x; 1.5x; 2x
•Manual
•6-fold or 7-fold for objectives with M25 thread
•Objective prism slide
•With condenser holder
•Coaxial pinion, optional: telescopable
•Controls mountable on left or right
4. Overview of the Instrument
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