Leica Stellaris 8 FALCON Guida all'installazione
Leica Stellaris 8 FALCON quick manual
Version 1.0
Start-up
1. Check which stage adapter is in place. If you need to change the stage
adapter, do it before you start the microscope.
The adapter on the left is meant for 96/24 wellplates and 3D-printed
sample holders. The adapter on the right is meant for regular objective
glasses and 35 mm dishes. If you are working with incubation, remember
to pluck the holes on the adapter using spare objective glasses / dishes
found next to the microscope.
2. Turn on the microscope and the laser by
pressing the two buttons on the large black
box left of the microscope. Also turn on the
fluorescent lamp.
You cannot see the switches, but you can
feel the above the key. You do not need to
touch the key unless it is not in the position
shown here.
3. Start the computer and login with your
credentials
4. Start the Las X software.
5. Chose the appropriate configuration. For the time being this is ‘machine’.
6. Once the software had fully started, you can put
in your sample.
7. Got configuration (see below) and then chose
laser configuration (see right)
If you have DAPI / Hoechst in your sample, turn on the Diode 405 laser. Then
turn on the white light laser (WLL).
8. Now get back to the Acquisition side to set up the excitation / detection
paradigm.
If you have any saved settings, you can load them from the button (A).
If you need to make a new scheme you can add new sequences form the (F)
button. Then type the colour you have in your sample in the search window (B)
and use mouse cursor to drag the desired dye from the dye bar (C) to the
location of the desired detector (D) or (E). Adjust detection ranges if necessary.
Stellaris has two types of detectors, HyD S and HyD X.
HyD S Not quite as sensitive for FLIM or photon counting as HyD X, but tolerate
more light. Use these for bright channels and when making a coverslip
correction.
HyD X Use these detectors for dim channels / for photon counting applications /
when doing FLIM. NEVER USE HyD X FOR COVERSLIP CORRECTION.
You can use widget (G) to visualize the excitation / emission spectrum of your
chosen dyes to better design your excitation / detection paradigm.
Heating and CO2
The environmental controller is on the floatation table, next to the microscope. To turn on
the heating press the on/off button. Adjust the temperature to either 37 C (red) or 29C
(orange) depending on your needs or the colour of the next booking with 24 hours. Do not
turn on the CO2 unless you need it yourself. 5% CO2 should be sufficient for most samples.
To turn off the heating, press the off button down for about five seconds. To turn off the
heating, press the on/off button for about two seconds and then confirming the shutdown
from the screen.
Shutdown and preparing the microscope for the next
user
1. Save your data.
2. Check who is coming next to the microscope and what lasers they need
3. Check from IRIS if the next user is coming in two hours or less.
If the next user is coming in 2 hours or less, use protocol A, else use
protocol B
A. Turn off the 405 laser from the laser configuration menu if you used it and
the next user does not need it.
Shut down the software.
Remove your sample and clean the objectives.
Log off from the computer.
Check from IRIS if the next user within 24 hours needs the microscope
heated. If you used heating, leave the heating on, adjusting temperature if
needed. If you did not use heating, turn the heating on, adjusting
temperature if needed.
If you used CO2 turn it off.
disinfect all the surfaces you touched.
B. Turn off both the lasers from the laser configuration menu.
Shut down the software.
Remove your sample and clean the objectives.
Shut down the microscope.
Shut down the fluorescent lamp.
Check from IRIS if the next user needs the microscope heated. If you used
heating, leave the heating on, adjusting temperature if needed. If you did
not use heating, turn the heating on, adjusting temperature if needed.
Shut the computer down.
If you used CO2 turn it off.
Disinfect all the surfaces you touched
Indice
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