LW Scientific M2-P-RH Manuale utente
Head
Binocular (Seidentopf)
Optional trinocular with C-mount or eye tube
35mm and digital camera adapters available
Diopter adjustment
Inclined 30° (45° available), rotates 360°
10X/20 high point eyepieces (10X/22 available)
Interpupillary distance range 50-75mm
Nosepiece
Reverse quadruple nosepiece
Multiple ball bearing mounted
Objectives
DIN Plan flat-field objectives
4X, 10X, 40XR, 100XR (oil)
Anti-fungal, parfocal, parcentric, color-coded
Stage
Mechanical stage (160mm x 140mm)
Coaxial drive controls, metal rack & pinion
Range of traverse: 77mm x 50mm
Stage locking lever
Slow-close hydraulic slide finger
Dual slide holder
Focus
Coarse adjustment: range of 20mm
Fine adjustment: graduation of 2µm
Tension control knob
Illumination
Polarizing kit included - see back for instructions
Moveable Abbe condenser, NA 1.25
Iris diaphragm
Halogen light source (12V/20W bulb)
Variable light adjustment: 0-20W output
Input 90-240V / 50-60Hz automatic switching
Accessories Included
One 2 amp fuse, spare 12V/20W bulb, blue, green,
and yellow filters, dust cover, immersion oil, manual
and warranty card
Options
Ceramic stage, dark field condenser, phase contrast
turret, and Koehler-type illumination
Warranty
Lifetime on materials and workmanship
One year on electronic components
Dimensions and Weight
Height: 16 1/2” (420mm)
Length: 10 5/8” (270mm)
Width: 7 7/8” (200mm)
Weight: 19.5 lbs. (9kg)
Laboratory Microscopes
The M2 Rheumatology LabScope is an essential tool
for synovial fluid analysis. Crystal-induced arthritis,
such as gout or pseudogout, can be identified and
differentiated with the use of the polarizing attachment
and red compensator lens. The Rheumatology
LabScope also functions as a flat-field, laboratory-
grade microscope for routine cytology, hematology,
urinalysis, etc. This specialized system was
designed to combine high-resolution optics with
functionality at an affordable price for the
Rheumatologist. The M2 LabScope provides comfort,
durability, dependability and superior imaging for the
lifetime of the laboratory, and the lifetime warranty
proves it.
Recommended upgrade:
Koehler Illumination
865 Marathon Parkway - Lawrenceville, Georgia 30045 - Ph: 770-270-1394 - Fx: 770-270-2389
Rheumatology LabScope
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Rheumatology Polarization
Installing and Operating Instructions
Introduction:
Polarized light microscopy is used for observation of crystalline and mineral specimens, because of their optically
anisotropic character. Contrast occurs due to polarized light interacting with the birefringence (double refraction) of
crystals. The polarizer is a two-stage filter assembly (polarizing lens and red compensator lens) that must be
positioned in the light path below the specimen, and the analyzer (smaller polarizing lens) must be positioned above
the specimen and objectives and beneath the head.
When the two filters are aligned parallel with each other, the polarized light path is transmitted through both filters and
the field will be at its brightest, but when the polarizer (lower) is turned 90 degrees the light is extinguished. At this
cross-polarized setting anisotropic crystals will appear to glow against a relative dark background, and when the red
compensator lens is used the birefringent material will show as either blue or yellow against a red background
depending on the material’s orientation.
Polarization is an investigative tool used to distinguish different types of arthritis. It has many other applica
tions as well.
Features:
The polarization kit is only available on M-Series LabScopes. It includes two parts which are easily installed in less
than 1 minute. These quality filters combined with the bright illumination and PLAN optics of the M-Series LabScopes
produce clear contrast and high resolution images.
Installation and Setup:
Remove the head of your M-Series LabScope.
Install the analyzer (the smaller filter...about the diameter of a quarter) by placing it into the small opening
inside the head mounting circle on the microscope. Make sure the small screw on the side of the filter fits
into the slot inside the opening. You may leave the analyzer in place for brightfield use if you choose.
Re-install your head.
Remove the red compensator lens (clear lens with plastic handle) from the lower polarizer assembly by
simply pulling it out of the housing. Set it aside.
Install the polarizer by loosening the thumb screw and placing it over the base illuminator lens. Turn on
the microscope, select the 10x or 40x objective, and look through the eyepieces to view the
light field. Turn the polarizer 90 degrees either direction to achieve the darkest light setting
and then tighten the screw. Make sure that the slot for the red compensator lens is not
facing to the rear.
Using a sample specimen slide, focus on an active crystal which should be glowing and then slide the red
compensator lens into place over the polarizer.
Rotate the red compensator lens to achieve a red background. Crystals will show either positive or
negative birefringence, by turning either yellow or blue in relation to their orientation. By rotating the red
compensator lens 90 degrees, you will notice the color of the crystals change from blue to yellow, or
yellow to blue.
Use positive test slides to create a protocol for diagnosing gouty or pseudo gouty arthritis. Crystals
should be identified by both their morphology and their direction of birefringence.
When finished, remove the polarizer and store carefully in a dust free container.
Care and maintenance:
Clean the filters very delicately with lens paper and lens cleaning solution only if dirty.
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