Nikon Ee1078 Manuale utente

Nikon Wide Field Microscope Ee1078

Quick start

Erasmus Optical Imaging Centre document version 3

Start-up system

•Switch on the power socket (#1)

•Switch on Mercury lamp control unit switch on and ignite (#2)

oWait ~10 seconds for the device to load

oHold ignite button until orange LED is active

•Start the PC before other hardware is active

oUser account Nikon1078, no password required

•Switch on the power socket (#3)

•Switch devices on in the order of:

oHalogen lamp

oIncubator control-box

oMicroscope stand located at the right, behind the serial plug

oStage controller located at the back of the device

oShutter controller

•Start imaging software Metamorph

oSelect "User" account

•Upon error; drag error message from behind

Metamorph logo

oMessage: ASI MS2000 not detected on COM1

oChoose: Retry

Shut down system

•For live cell imaging:

oRemove Tokai Hit incubator from the stage and place it on the table

oClose the Tokai Hit incubator with a plastic well

•Clean objective carefully

oRemove oil with lens paper:

oHold lens paper tight between your fingers and wipe over objective

oWet your lens paper with 2-propanol and wipe 3-4 times more

•Cover stage with lens paper cleaning box

•If you are the last user of the day

•Shut down all devices

oShut down Mercury lamp at last

oCamera power is switched at the adapter box at the floor

•Switch off the power socket located at the floor

Nikon Wide Field Microscope Ee1078

Quick start

Erasmus Optical Imaging Centre document version 3

Controlling intensity mercury lamp

•Fluorescence can be detected with use of the Mercury lamp

oChoose illumination settings according to your sample

•Intensity of the light can be controlled with neutral density (ND) filters.

oLocated between the actual lamp (right) and the microscope stand.

oPress inwards to reduce intensity by 4 or 8 times (ND4, ND8)

Taskbar

•When the taskbar is not visible

oLoad the taskbar via: Journals>Taskbar>Load Taskbar

oLocation C:\MM\TASKBARS\

Restore

illumination

settings

Open acquire window

Open stream

window

Open MDA configuration

Move stage with cursor

Select

illumination

setting to

eyepiece

Select illumination

setting to camera and

start Live mode

•If needed: reset the illumination settings with: "Restore OIC settings"

•Open the Acquire window

•Configure Stream acquisition or start MDA configuration

•Move stage by clicking inside live image window (distance from centre equals

movement)

•Select illumination settings for Eyepiece (left) or camera (right)

Nikon Wide Field Microscope Ee1078

Quick start

Erasmus Optical Imaging Centre document version 3

Acquire window

•Open via Taskbar button “Acquire”

•Acquire an image

•Set exposure time

•Camera gain settings

•Show/Stop live view

Multi-Dimensional Acquisition (MDA)

Combine multiple tasks: z-stack, timelapse, streaming, multiple positions, multiple

wavelengths, use of journals.

Select acquisition mode(s) in Main

•Continue with the tabs shown

left:

oSaving: select filename and

folder

oSelect illumination settings

o… options for acquisition

modes

•When setup is done press acquire

•Save or load settings of

experiment setup, with Save/Load

State buttons

•Live mode can be started from MDA window

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