NPI EXT-10C Istruzioni operative

OPERATING INSTRUCTIONS AND

SYSTEM DESCRIPTION FOR THE

EXT-10C

EXTRACELLULAR AMPLIFIER

MODULE FOR EPMS SYSTEMS

VERSION 2.0

npi 2012

npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany

Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240

[email protected]; http://www.npielectronic.com

EXT-10C User Manual

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Table of Contents

1. Safety Regulations............................................................................................................ 3

2. EPMS-07 Modular Plug-In System.................................................................................. 4

2.1. Components of the EPMS-07 Housing ....................................................................... 4

2.2. General System Description / Operation..................................................................... 4

2.3. EPMS-E-07 Housing ................................................................................................... 4

2.4. System Grounding ....................................................................................................... 5

2.5. Technical Data.............................................................................................................5

3. EXT-10C .......................................................................................................................... 6

3.1. EXT-10C Components ................................................................................................ 6

3.2. System Description...................................................................................................... 6

3.3. Signal Flow Diagram................................................................................................... 6

3.4. Description of the Front Panel..................................................................................... 7

3.5. Headstage .................................................................................................................... 9

Headstage Elements..................................................................................................... 9

3.6. Operation ..................................................................................................................... 9

4. Literature .......................................................................................................................... 11

Technical Data......................................................................................................................... 12

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1. Safety Regulations

VERY IMPORTANT:Instruments and components supplied by npi electronic are NOT

intended for clinical use or medical purposes (e.g. for diagnosis or treatment of humans),

or for any other life-supporting system. npi electronic disclaims any warranties for such

purpose. Equipment supplied by npi electronic must be operated only by selected,

trained and adequately instructed personnel. For details please consult the GENERAL

TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic, D-71732

Tamm, Germany.

1) GENERAL: This system is design ed for use in scientific laboratories and m ust be

operated only by trained staff. General safety regulations for operating electrical devices

should be followed.

2) AC MAINS CONNECTION: While working with the npi systems, always adhere to the

appropriate safety m easures for handling el ectronic devices. Before using any device

please read manuals and instructions carefully.

The device is to be operated only at 115/230 Volt 60/50 Hz AC. Please check for

appropriate line voltage before connecting any system to mains.

Always use a th ree-wire line cord and a m ains power-plug with a p rotection contact

connected to ground (protective earth).

Before opening the cabinet, unplug the instrument.

Unplug the instrum ent when replacing the fu se or changing line voltage. Replace fuse

only with an appropriate specified type.

3) STATIC ELECTRICITY: Electronic equipm ent is sensitive to static discharges. Some

devices such as sensor inputs are equipped with very sensitive FET amplifiers, which can

be damaged by electrostatic charge and must therefore be handled with care. Electrostatic

discharge can be avoided by touching a gr ounded metal surface when changing or

adjusting sensors. Always turn power off when adding or removing modules,

connecting or disconnecting sensors, headstages or other components from the

instrument or 19” cabinet.

4) TEMPERATURE DRIFT / W ARM-UP TIME: All analog electron ic systems are

sensitive to temperature changes. Therefore, all electronic instruments containing analog

circuits should be used only in a warmed-up condition (i.e. after internal temperature has

reached steady-state values). In most cas es a war m-up period of 20-30 m inutes is

sufficient.

5) HANDLING: Please p rotect the d evice from moisture, h eat, radiation and corrosive

chemicals.

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2. EPMS-07 Modular Plug-In System

2.1. Components of the EPMS-07 Housing

The following items are shipped with the EPMS-07 housing:

 EPMS-07 cabinet

 Power cord

 Fuse 2 A / 1 A, slow

 Front covers

2.2. General System Description / Operation

The npi EPMS-07 is a m odular system for processing of bioe lectrical signals in

electrophysiology. The system is housed in a 19” rackmount cabinet (3U) containing a power

supply and has room for up to 7 plug-in units. The plug-in units are connected to power by a

bus at the rear panel.

The plug-in units m ust be kept in position by four screws (M 2,5 x 10). The screws are

important not only for m echanical stability but also for prope r electrical connection to the

system housing. Free area must be protected with covers.

In order to avoid induction of electrom agnetic noise the power supply unit, the power switch

and the fuse are located at the rear of the housing.

2.3. EPMS-E-07 Housing

The EPMS-E-07 housing is designed for low-noi se operation, especially for extracellular

amplifiers and filters which ar e plugged in. I t operates with an exte rnal power su pply to

minimize distortions of the signals caused by the power supply.

The external power supply PWR-03D supports up to 3 EPMS-E housings and each housing is

connected to the rear panel of the EPMS-E-07 by means of an 6-pole cable. An LED indicates

that the PWR-03D is powered on. P ower switch, voltage selector and fuse are located at the

rear panel.

Figure 1: PWR-03D front panel view

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Figure 2: PWR-03D rear panel view

Note: This power supply is intended to be used with npi EPMS-E systems only.

2.4. System Grounding

The 19" cabinet is grounded by the power ca ble through the ground pin of the m ains

connector (= protective earth). In order to avoid ground loops th e internal ground is isolated

from the protective earth. The internal gr ound is used on the BNC connectors or G ROUND

plugs of the m odules that are inserted in to the EPMS-07 housing. The internal ground and

mains ground (= protective earth) can be conne cted by a wire using the ground plugs on the

rear panel of the instrument. It is not possible to predict whether measurements will be less or

more noisy with the internal ground and mains ground connected. We recommend that you try

both arrangements to determine the best configuration.

2.5. Technical Data

19” rackmount cabinet, for up to 7 plug-in units

Power supply: 115/230 Volts AC, 60/50 Hz, fuse 2 A / 1 A slow, 45-60 W

External power supply (for EPMS-E): 115/230 Volts AC, 60/50 Hz, fuse 1.6/0.8 A, slow

Dimensions: 3U high (1U=1 3/4” = 44.45 mm ), 254 mm deep (with high voltage power

supply: 354 mm deep)

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3. EXT-10C

3.1. EXT-10C Components

The following items are shipped with the EXT-10C system:

 Amplifier module for the EPMS-07 system

 GND- (2.6 mm banana plug) and REF. (SMB) connectors for headstage

 Headstage

 User manual

3.2. System Description

The EXT-10C was designed for perform ing low noise recordings of small extracellular

signals in slices or in in vivo preparations using fine tipped glass or m etal microelectrodes.

The system consists of a module for the npi EPMS-07 modular system and a small headstage

with a holding bar.

The EXT-10C has a differential input with high input resistance for minimizing noise and the

incoming signal can be compensated for offset and capacity of the microelectrode by the two

potentiometers OFFSET and C-COMP. An anal og balance m onitor makes the control of

offsets easy. The recorded signal can be obtai ned either AC with GAIN of 100, 1k or 10k or

DC with GAIN of 10, 100 or 1k. An optional NOTCH filter eliminates 50/60 Hz noise in AC

measurements.

The EXT-10C is designed to be used with the DPA-2F pr eamplifier/filter module from npi

which combines additional gain stages with high- and lowpass filters.

3.3. Signal Flow Diagram

The signal is passed through the EXT-10C as shown below.

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3.4. Description of the Front Panel

Figure 3: EXT-10C front panel view

In the following description of the front panel elements each elem ent has a number tha t is

related to that in Fi gure 3. The number is followed by th e name (in uppercase letters) written

on the front panel and the type of the element (in lowercase letters). Then, a short description

of the element is given.

(1) HEADSTAGE connector

The HEADSTAGE cable is connected to the unit at an 8-pin connector in the center

of the module.

(2) DC OUTPUT connector

BNC connector providing the am plified signal DC coupled. The a mplification factor

is set by the GAIN DC switch (3). The DC OUTPUT is always unfiltered.

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(3) GAIN DC switch

Switch to set the GAIN of the outgoing DC signal. Three GAIN factors are

available: x10, x100 or x1k.

(4) C-COMP. potentiometer

Control for com pensation of stray capaciti es of the m icroelectrode (10 turn

potentiometer, range 0-20 pF). Due to the positive feedback circuit

overcompensation of the capacity will lead to oscillations which may damage the

cell.

(5) POTENTIAL x10 meter

The analog POTENTIAL monitor displays the offset in the range of 30 mV

and is used for optimal cancellation of the OFFSET.

(6) –OVER +OVER LEDs

LEDs that indica te if the am plifier 10% below it’s positive or negative lim it

(±10 V). The linear range of the amplifier is 12 V.

(7) OFFSET potentiometer

10 turn potentiometer to compensate for the OFFSET of a DC signal.

Note: Position 5 on the OFFSET control corresponds to 0 mV offset.

(8) GAIN AC switch

Switch to set the GAIN of the outgoing AC signal. Three GAIN factors are

available: x100, x1k or x10k.

(9) AC OUTPUT connector

BNC connector providing the am plified signal AC coupled. The a mplification factor

is set by switch (8).

(10) NOTCH switch (optional)

Switch to set the NOTCH filter (50 Hz / 60 Hz ) or to bypass the NOTCH filter (switch

position OFF) for AC measurements.

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3.5. Headstage

Figure 4: electrode holder (optional) and headstage of the EXT 10-2F

Headstage Elements

1 PEL:BNC connector for the electrode holder (measuring electrode)

2 REF: SMB connector for the reference electrode

3 GND: Ground connector

4 Holding bar

On request, P EL can be im plemented using 1 mm or 2 mm banana jacks or using SMB

connectors.

Very Important: EXT 10C headstages are always labeled “EXT” (see Figure 4) and must not

be exchanged with headstages from npi electronic’s desktop EXT amplifiers, e.g. the EXT-2F

which is labeled “EXT-02”!

Also Important: The shield of the BNC connector of the headstage is connected to driven

shield, and must not be connected to ground.

3.6. Operation

Extracellular measurements are mostly done in slices or in vivo, where distortions of the

signal caused by other instrum ents and the animal itself are very co mmon. Additionally,

extracellular signals are very sm all and have to be a mplified enormously. The drawback is

that noise is amplified as well. Ther efore, the headstage of the EXT- 10C is equipped with a

differential input that m inimizes noise pick-up. Differential m eans, that the signal for the

amplifier is the difference between the positive ( +) (PEL) and negative (-) (REF.) input of the

headstage. This results in canceling of all signals which both electrodes record, e.g. noise.

For differential measurements, both inputs of the headstage (REF. and P EL) are connected to

microelectrodes using cables with grounde d enclosure or electrode holders. P EL is connected

to the measuring electrode and REF. to the re ference electrode. The experimental chamber is

grounded by an Ag-AgCl pellet (or an AGAR br idge) connected to G ND of the headstage

(see Figure 5).

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If differential m easurement is not required (single-ended measurement configuration, see

Figure 5), the REF inp ut must be connected to ground (GND). The amplifier is in an

undefined state, if the REF is left open, an d can go into saturation m aking reliable

measurements impossible.

Figure 5: headstage connections, A: differential measurement, B: single-ended measurement

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