Olympus CHD Manuale utente

scanned by

McHone

Jan 2010

for personal use only, not for sale

BIOLOGICAL

MICROSCOPE

This

instruction

manual

for

use

the

Olympus

Biological

Microscope

Model

CHD.

recommend you read this

manual

carefully

order

familiarize

yourself

fully

with

the

use

your

microscope so

that

you

can obtain

maximum

performance.

OLYMPUS

AX!)570

BEFORE

USE

Observe the

following

procedures carefully:

Operation

------------------------

~---_-.I

Since the microscope

aprecision instrument, always handle it

with

care, and avoid

abrupt

motions

impacts.

(i)

Avoid exposure to direct sunlight, high temperature and

humidity,

dust and vibration.

@Never direct the sunlight to the microscope

mirror

while adjusting illumination in order

protect

your

eyes

from the sunlight.

@Only

use

the tension adjustment ring

for

altering the tension

the coarse adjustment knobs.

careful

not

to soil lens surfaces

with

dust, fingerprints, etc.

Main18nance

and

.;;..Sto...;....ra

Use

aclean brush or

lens

tissue paper to clean the lens surfaces.

the lens surfaces are soiled

with

oil or

fingerprints, wipe them

off

carefully

with

gauze moistened

with

asmall amount

alcohol and ether

(3:

solution, or xylene.

not

use

organic solutions

(e.g.

thinner, xylene, ether, alcohol,

etc'!

to wipe painted surfaces

various

components, and especially, plastic parts. When extremely soiled, they shou

cleaned

with

aneutral

detergent.

not disassemble any part

microscope, since the integrated performance may

impaired.

@When not in

use,

the microscope should

covered

with

the dust cover provided

contained in astorage

case,

and kept in aplace free

from

humidity

and mold.

Attaching

the

fixing blocks of the microscope

stand

the wooden ilqrege

case

ICHD-WBI

(optionally

avail'able,

The

fixing

blocks should

screwed at the

bottom

the storage

case

in the

following

procedure:

Insert the screw

into

aflat washer and one

the

two

holes

mm dia.) in the

base

plate

the

case

illustrated

the right.

2) Tighten the screw to clamp one block

means

the spanner provided.

3) Clamp the other block on the opposite side.

Fixing

block

Storage

case

Flat

washer

BEFORE

USE

Observe the

following

procedures carefully:

Operation

Since the microscope

aprecision instrument, always handle

with

care, and avoid

abrupt

motions

impacts.

Avoid exposure to direct sunlight, high temperature and

humidity,

dust and vibration.

@Never direct the sunlight to the microscope

mirror

while adjusting

illumination

in order

protect

your

eyes

from the sunlight.

@Only

use

the tension adjustment ring

for

altering the tension

the coarse adjustment knobs.

careful

not

to soil lens surfaces

with

dust, fingerprints, etc.

Maintenance

and

Stora

Use

aclean brush

lens

tissue paper to clean the lens surfaces.

the lens surfaces are soiled

with

oil or

fingerprints, wipe them

off

carefully

with

gauze moistened

with

asmall amount

alcohol and ether

(3:

solution, or xylene.

not

use

organic solutions

(e.g.

thinner, xylene, ether, alcohol, etc,) to wipe painted surfaces

various

components, and especially, plastic parts. When extremely soiled, they should

cleaned

with

aneutral

detergent.

not disassemble any part

microscope, since the integrated performance may

impaired.

@When not in

use,

the microscope should

covered

with

the dust cover provided

contained in astorage

case,

and kept in aplace free

from

humidity

and mold.

Attaching

the

fixing blocks of the

microscope

stand

the

wooden

storage

case

ICHO·\)VB)

(optionally available)

The

fixing

blocks should

screwed at the

bottom

the storage

case

in the

following

procedure:

Insert the screw

into

aflat washer and one

the

two

holes

mm dia.) in the

base

plate

the

case

illustrated

the right.

2) Tighten the screw to clamp one block

means

the spanner provided.

3) Clamp the other block on the opposite side.

Fixing

block

Storage

case

Flat

washer

CONTENTS

STANDARD EQUIPMENT 1I

2. SPECIFICATIONS 22

3. NOMENCLATURE 33

ASSEMBLY 4

14-1

AS$embly'

DIagram

14-~

Expranation for Assembly

Pf'ocedIJre

OBSERVATION (Putting the Microscope

Operation) 6

DSpecimen Placement 6

fJ Adjustment

the Microscope Mirror 7

Focus 7

Interpupillary Distance Adjustment

(for

CH-BI45-W only) 7

Diopter Adjustment 8

Objective selection 8

Adjustment

the Aperture Iris Diaphragm 8

Tension Adjustment

the

Coarse

Adjustment Knobs 9

III Locking

the Pre-focusing Lever 9

Use

Immersion Objectives

6. OPTICAL

DATA

7. TROUBLE SHOOTING

"--_----I

I-

a..

:::l

a::

STANDARD

EQUIPMENT

CHO·

Component

212E

012E

OOlE

Microscope stand

with

quadruple revolving nosepiece,

square plain

stage,

including dust plug

AA7808,

fiIter CHO-F 00 0

32.5C-2, immersion oil

Bcc

and dust cover

COll

Monocular observation tube, inclined 45° CH-M045-W 0 0

Binocular observation tube, inclined 45° CH-BI45-W 0

Attachable mechanical

stage

with

right-hand

low

drive CH-MVR 00

controls

Stage

clips, paired CH-SCBI 0

Condenser, N.A. 1.25 CH2-CO 0 0 0

Filter

holder CH2-FH 00 0

Plano-concave

mirror

K-MM 0 0 0

E 0 achromatic objective

ED4X 0

E 0 achromatic objective

lOX

E010X

000

E 0 achromatic objective

40X

(spring)

E040X/R

0 0 0

E 0 achromatic objective 100X (spring,

oil)

E0100X/RO

LB eyepiece

lOX

CWHK10X 00

LB eyepiece

10X(2

pcs.) CWHK10X 0

Optional accessories:

Phase

contrast attachment

Simple

phase

contrast attachment

Vertical

illuminator

Attachable mechanical stage

with

left-hand

low

drive controls

Sub-stage

ilium

inator

Table stand

illuminator

Wooden storage

case

CH2-PCO-PL

CH2-PC-PL

BH2-KMA

CH-MVL

LSK-3

LSO-W

CHO-WB

D----------------------------

SPECIFICATIONS

Item Description

Microscope Circular dovetail mount for observation tube; built-on quadruple

nose-

limb piece; and square plain

stage

124mm x153 mm.

Coarse

and fine Coaxial

coarse

and fine focusing controls.

Coarse

and fine drive

range

Microscope adjustments

25mm;

fine drive knobs graduated

increments

2.5J1..

Tension adiust-

stand ment knobs, and pre-focusing lever for

coarse

focusing.

Condenser holder Rack

and

pinion condenser height displacement up to 28mm.

Base

Provided

with

accommodation for attachment

microscope

mirror

and

substage illuminator.

Monocular Inclined 45°.

Observation Inclined 45°. Interpupillary distance adjustment

with

scale

between

tubes Binocular

53mm

-72

mm.

Variable diopter adjustment ring equipped on the left-side eyepiece tube.

Attachable mechanical

stage

Low-positioned coaxial

control

knobs; X-V

trave~sing

area

76mm

50 mm, compatible

with

two

standard slides simultaneously.

N.A. 1.25 (in immersion oil).

with

graduated aperture diaphragm. Pro-

Condenser vision to accept afilter holder.

Filter holder Accepts a32.5

dia. filter.

Microscope mirror Plano-concave

mirror

(50mm dia.l

Filter Blue

filter

(32.5mm dia.)

Objectives

ED4X,

EDlOX,

ED40X (spring-loaded). ED100X (spring-Ioaded, oiIim-

mersion)

Eyepiece CWHK10X (provided

with

accommodation to accept

eyepiece micro-

meter)

Dimensions 180 mm

(W)

x223 mm (D) x370 mm (H) (binocular version)

Eyepoint height Binocular: 372 mm Monocular: 375 mm

Weight CHD-212E: 4.9

kg.

--------------------------fJ

("")

a::

::>

I-

...J

NOMENCLATURE

Diopter

adjustment

ring

Binocular

tube

IRevolving nosepiece I

IObjective

Specimen holder

Mirror

Hand reset

IStage

clip

Condenser height

adjustment

knob

Pre-focusing lever

IEyepiece I

Observation

tube

clamping screw

Mechanical stage I

IMicroscope stand

Tension

adjustment

ring

Fine

adjustment

knob

Graduated in

increments

2.5/J.

Coarse

adjustment

knob

Coaxial

low

drive

control

knobs

Monocular

tube I

Condenser

clamping

screw

ICondenser I

Aperture

iris diaphragm

lever

Filter

holder

Accepts

32.5mm

dia.

filter.

IJ-----------------------

--------

ASSEMBLY

[4-1

Assembl,V

Diagram

*Assemble

each

component

the order

the numbers, taking care to keep all

glass

surfaces clean and avoid

scratching the lens surfaces,

Objective

-------f

clip

Mechanical

stage

Filter

(32,5

dia.)

condenser.g

Filter

holder

Microscope

stand

(f)

4-2

Exp'lanation for

Assembl,V

Procedure

Fig.

DMounting the observation tube

Loosen the clamping screw

fully,

and

mount

the observation

tube

the circular dovetail

mount.

Reclamp the screw

securely

hold

the observation tube

the

stand. (Fig.

The

observation tube can

normally

mounted

the stand

with

the

eyepiece tube(s)

pointing

backward the microscope

that

the observer

can

look

into

the eyepiece(s) over

the

pillar

utilize

available

light

much

possible; however,

the

observation

tube

can be clamped at any

direction

the observer preference

convenience.

Insertion

the eyepiece(s)

Insert

the

eyepiece(s)

into

the

eyepiece tube(s)

(~).

(Fig. 1)

-----------------------------11

Fig.

(J)

Use

eyepiece

micrometer

eyepiece

micrometer

(10

mm/100~

(optionally

available) can

inserted

into

the eyepiece

CWHK10X

in the

following

procedure:

1) Remove the retaining

ring'

1./

from

the lower end

the eyepiece

and place the micrometer on the retaining ring

with

the

reticle-

engraved surface, facing

downward.

(Fig. 2)

certain

clean the

micrometer

disc before inserting

into

the

eyepiece.

Return the retaining ring

into

the eyepiece and insert the eyepiece

into

the eyepiece tube.

Fig.

III Mounting

the

objectives

1) Lower the stage by means

the coarse adjustment knobs.

Screw the objectives

into

the nosepiece,

from

low

power

to higher

power in aclockwise

direction.

(Fig.

*Close the

empty

aperture in the nosepiece

with

aplug

provided.

(Fig. 3)

Mounting

the

mechanical

stage

Mount

the mechanical stage on the plain

stage

in amanner

that

the

specimen traversing guide

the mechanical stage

located

closest

the

pillar,

and tighten the stage clamping screws ®

with

acoin. (Fig. 4)

•Insertion

stage clips

The plain stage

pre-drilled

for

insertion

the stage clips when the

mechanical stage

not

attached

(see

page

4).

Mounting

the

condenser

Insert

the

condenser

into

the condenser

holder,

with

the condenser

iris diaphragm lever

(1),

directing in the microscope

front,

and

tighten

the clamping screw (2) .(Fig. 5)

11----------------------------

Insertion

the blue filter

Slip the blue

filter

(32.5mm

dia.) ®

into

the

filter

holder

CD.

(Fig.

Insertion

the filter holder

Insert the

filter

holder m

into

the condenser

from

below. (Fig. 6)

Fig.

...

Mounting the mirror

Insert the

mirror

C:D

fork

into

the

mirror

mounting

seat ®in the

base.

the

mirror

fork

loose,

pullout

the

mirror

fork,

and adjust the

slit in the

fork

with

ascrewdriver.

Fig.7

oBSE

RVAT

ION

(Putting the Microscope

Operation)

(Il

:Xl

-I

Fig.

DSpecimen Placement

Open the spring-loaded specimen finger

CD,

and slide the specimen

slide

into

the holder,

with

the cover

glass

upward.

Push

the slide all the way against the holder and

release

the finger

slowly. (Fig. 8)

sudden

release

the

finger may cause damage

the

slide

the

holder.

2) For

use

the

stage

clips

place

the specimen holder, insert apair

stage clips

into

the holes on the

drilled

surface

the stage and

insert

the

specimen slide between

each

clip and the stage surface.

•

Use

acover

glass

Use

acover

glass

0.17mm

thick

conjunction

with

the objectives marked

with

the

inscription

"160/

0.17"

for

optimum

performance

these objectives.

•

Use

aspecimen slide

The thickness

aspecimen slide between 0.9 mm and 1.2 mm

recommended for the CHD.

the

thick-

ness

aslide exceeds this range,

illumination

may sometimes

impaired.

---------------------------B

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