OPTIKA MICROSCOPES B-500POL-I Manuale utente
OPTIKA MICROSCOPES - ITALY
Ver. 1.0.0
B-500POL-I
OPERATION MANUAL
GUIDA UTENTE
MANUAL DE INSTRUCCIONES
Page 2
INDEX
1.0 DESCRIPTION page 4
2.0 INTRODUCTION page 6
3.0 UNPACKING AND ASSEMBLY page 6
4.0 USING THE MICROSCOPE page 9
5.0 MAINTENANCE page 14
6.0 ELECTRICAL SPECIFICATION page 14
7.0 RECOVERY AND RECYCLING page 15
Page 3
This microscope is a scientic precision instrument designed to last for many years with a minimum of main-
tenance. It is built to high optical and mechanical standards and to withstand daily use.
Optika reminds you that this manual contains important information on safety and maintenance, and that it
must therefore be made accessible to the instrument users.
Optika declines any responsibility deriving from instrument uses that do not comply with this ma-nual.
Safety guidelines
This manual contains important information and warnings regarding safety about installation, use and
maintenance of the microscope. Please read this manual carefully before using the equipment. To
ensure safe use, the user must read and follow all instructions in this manual. OPTIKA products are
designed for safe use in normal operating conditions. The equipment and accessories described in
the manual are manufactured and tested according to industry standards for safety instrumentation
laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand
close to the instrument, for an easy consultation.
Electrical safety
Before connecting the power cord to wall outlet, ensure that your mains voltage for your region corre-
sponds to the voltage supply of the instrument, and that the illuminator’s switch is in position OFF. The
user must observe the safety regulations in force in his region. The instrument is equipped with CE
safety marking, in any case the user has full responsibility concerning the safe use of that instrument.
Warning/Caution symbols used in this manual
The user should be aware of safety aspects when using the instrument. Warning or hazard symbols
are shown below. These symbols are used in this manual.
The instructions on this symbol to avoid possible severe personal injuries.
Warning of use; the incorrect operation on the instrument can cause damages
to the person or instrument.
Possibility of electric shock.
Attention: high temperature surfaces. Avoid direct contact.
Technical notes or usage tips.
SAFETY GUIDELINES
Page 4
1.0 DESCRIPTION
PHOTO PORT
LEVER
EYEPIECE
TINT PLATE
HOLDER
FIELD
DIAPHRAGM
STAGE LOCK
KNOB
OBSERVATION
HEAD
NOSEPIECE
APERTURE
DIAPHRAGM
TENSION
ADJUSTMENT
KNOB
POLARIZER
ANALYZER
POLARIZER
REFLECTED/
TRANSMITTED
LIGHT SELECTION
FIELD
DIAPHRAGM
APERTURE
DIAPHRAGM
Page 5
1.0 DESCRIPTION
STAGE
DIOPTRIC ADJUSTMENT
RING
BERTRAND LENS
ATTACHMENT
CONDENSER
CENTERING
KNOBS
BRIGHTNESS
ADJUSTMENT
KNOB
CONDENSER
ADJUSTMENT
KNOB
FOCUS-STOP
KNOB
DIAPHRAGMS CENTERING
SCREWS
FINE FOCUSING
KNOB
COARSE
FOCUSING KNOB
Page 6
This microscope is a scientic precision instrument designed to last for many years with a minimum
of maintenance. It is built to high optical and mechanical standards and to withstand daily laboratory
use.
Optika reminds you that this manual contains important information on safety and maintenance, and
that it must therefore be made accessible to the instrument users.
Optika declines any responsibility deriving from instrument use that does not comply with this
manual.
3.0 UNPACKING AND ASSEMBLY
3.1 The microscope is housed in a moulded Styrofoam container. Remove the tape from the
edge of the container and lift the top half of the container. Take some care to avoid that the
optical items (objectives and eyepieces) fall out and get damaged. Using both hands (one
around the arm and one around the base), lift the microscope from the container and put it on
a stable desk.
3.2 The objectives are located in individual protective vials. Remove the objectives from the vials
and insert them into the microscope nosepiece in the order from the lowest magnication to
the highest, in a clockwise direction from the rear.
3.3 Insert the reected light attachment onto the top of the microscope stand and lock its screw.
Connect the mains plug into the socket at the base
The power cord should be used only on network sockets equipped with adequate grounding.
Contact a technician to check the state of your electrical system. If there is no need to install
additional accessories, the instrument is now ready for use. Once positioned and installed
with the necessary components, the microscope is ready to be used. Your microscope is a
laboratory instrument designed to last. Handle it always carefully and avoid abrupt vibrations
or shocks. Always disconnect the power cable from the microscope when not in use for long
time, while you clean it or when you perform any maintenance.
AVOID DISASSEMBLING THE INSTRUMENT
Do not disassemble the instrument. This entails the cancellation of the warranty and may
cause
malfunction.
2.0 INTRODUCTION
Page 7
FIXING SCREW
3.4 Connect the reected light cable to the back side of the microscope.
3.5 Put the Bertrand lens attachment on the reected light illuminator and rmly x it using the
provided allen wrench
3.6 Insert the observation head onto the top of the Bertrand lens attachment and lock its screw.
Insert your adaptor in the hole on the top of the observation head and xate it with the
screw on the side:
3.7 Insert the eyepieces into the eye tubes.
3.0 UNPACKING AND ASSEMBLY
Page 8
3.8 Insert the condenser into its holder and lock its screw.
3.0 UNPACKING AND ASSEMBLY
Page 9
COMMON SETTINGS FOR TRANSMITTED AND INCIDENT LIGHT
4.1 Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation,
and then lock the lock-screw.
4.2 Place the specimen on the stage
Fix the specimen slide on the mechanical stage using the slide-clamp. Ensure that the
specimen is centered over the stage opening.
4.3 Lamp settings
In order to activate the transmitted light illuminator, insert the plug of the mains cable into
the socket and turn on the switch on the back of the main body. Turn the brightness adjust-
ment knob to a brightness suitable for observation.
4.4 Preliminary settings
Before trying to focus the sample, ensure that:
The eld diaphragm is fully open.
The aperture diaphragm is fully open.
No lter is inserted under the observation head.
The analyzer lter is in OUT position (lever fully pulled out).
Photo port closed (lever fully in).
Bertrand lens in OUT position (you read “0” on the Bertrand lens disc under the observation
head). Swing out lens of the condenser in OUT position (if you use the 4X objective).
4.5 Adjust interpupillary distance
Hold the right and left parts of the observation head using both hands and adjust the interpu-
pillary distance by turning the two parts until one circle of light can be seen.
4.6 Focus and diopter adjustment
Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with
a 4X or 10X objective. Adjust the ne focusing knob to get the image sharp and clear while
observing with your right eye, then turn the left diopter ring to a sharp and clear image also
with the other eye. Turn the tension-adjust-knob to get a suitable tension for the focus system.
4.7 Align the system
Transmitted light: Insert the swing out lens of the condenser and fully close the eld dia-
phragm. Rotate the condenser adjustment knob until you see a sharp image of the closed
eld diaphragm (a bright spot of light). Act on the condenser centering screws until you move
the bright spot in the center of the image eld. Then re-open the eld diaphragm.
Incident light: Completely close the eld diaphragm and act on its centering screws in order
to put the bright circle in the eld center. Then open the eld diaphragm and close the aper-
ture diaphragm: act on its centering screws in order to have a centered distribution of light in
the eld (no signicant vignetting should be noticed along the eld edge).
4.0 USING THE MICROSCOPE
Page 10
4.8 Center the stage
With the Bertrand lens out, focus on your slide. Center the optical axis of the objective you
are using with the rotation axis of the stage using the two centring screws above each ob-
jective.
A fairly easy method of centring is the following: look at your sample while continuously
rotating the stage clockwise and then counter-clockwise by a little angle (e.g. 30° or 45°).
During these oscillations you should spot a point on the sample that doesn’t rotate on a
circumference but only revolving about itself. With the two objective’s centering screws,
bring this point in the center of the eld of view. In this way the mechanical center of rotation
of the stage coincides with the system’s optical axis. The stage can be locked in its position
using the stage lock knob.
4.9 Set the numerical aperture
Adjust the aperture of the iris diaphragm under the condenser to set the numerical aperture
of the illuminator, thus controlling image contrast and resolution.
4.10 Bertrand lens
In order to have a conoscopic view of your sample, you can insert a Bertrand lens in the
light path by rotating the disc under the observation head.
The Bertrand lens can be ne aligned along Z axis by rotating the knurled focusing knob.
The lens can also be centered in X-Y axis by means of two screws located at the back of
the lens.
“B”: BERTRAND LENS IN
“0”: BERTRAND LENS OUT
BERTRAND LENS Z- AXIS
FOCUSING KNOB
INSERT HERE THE PROVIDED
SCREWDRIVERS
TO CENTER THE OBJECTIVE
4.0 USING THE MICROSCOPE
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