PHYWE MIC-200 Series Manuale utente

Operating instructions

Student microscopes MIC-200 Series

MIC-222, MIC-231

PHYWE Systeme GmbH & Co. KG

Robert-Bosch-Breite 10

D-37079 Göttingen

Telefon +49 (0) 551 604-0

Fax +49 (0) 551 604-107

E-mail [email protected]

Internet www.phywe.com

Fig. 1:MIC-222 (left) und MIC-231 (right)

The unit complies

with the correspond-

ing EC guidelines.

INHALTSVERZEICHNIS

1INTRODUCTION

2CONSTRUCTION OF THE MICROSCOPE

3PREPARING THE MICROSCOPE

4FUNCTIONS OF THE MICROSCOPE

5WORKING WITH THE MICROSCOPE

6MAINTENANCE AND CLEANING

7TECHNICAL DATA

8WASTE DISPOSAL

1 INTRODUCTION

By purchasing a microscope from the MIC-200 series, you

have chosen a quality microscope. The MIC-200 series mi-

croscopes are designed for use in schools and university

student labs as well as and laboratories.

This instruction manual describes how to set up the micro-

scope, how to use, clean and maintain it.

The MIC-200 microscopes differ as follows:

Model

Binocular / trinocular

MIC-222

Binocular

MIC-231

Trinocular

2 CONSTRUCTION OF THE MICROSCOPE

After completing the step "Preparing the microscope", first

familiarize yourself with the mechanical, but also the optical

and electrical components of your microscope. Operate each

component carefully by hand and get an impression of the

functionality of each adjustment option.

The names of the components are listed here and are

marked in the Fig. 1 & 2.

A1) Eyepiece

A2) Revolving nosepiece for 5 objectives

A3) and A4) Mechanical stage with specimen holder

A5) Abbe condenser

A6) Iris diaphragm

A7) Condenser holder

A8) Filter holder

A9) Polarizer (optional)

A10) Binocular head

A11) Head lock screw

A12) Main body

A13) Phase contrast slide / darkfield slide slot

A14) and A15) Coaxial drive for X and Y movement of the

sample holder

A16) Light intensity adjustment knob

B1) 1X C-mount (MIC-231)

B2) Trinocular observing tube

B3) Lifting handle

B4) On/off switch

B5) Fuse socket

B6) Power socket

B7) Diopter adjustment ring on left eyepiece

B8) Objectives (4x, 10x, 40x, 100x)

B9) Focusing knob of condenser

B10) Adjustment handwheel of coarse focusing mechanism

B11) and B12) Coaxial coarse and fine focusing mechanism

3 PREPARING THE MICROSCOPE

Unpack the microscope and place it on a table. Take out the

main body and observing tube, connect them together, and

then use the attached Allen wrench to tight the observing

tube lock screw.

The objectives 4x, 10x, 40x and 100x objectives are pre-

assembled with this microscope.

Plug in the power plug and switch on the microscope. Now sit

comfortably in front of the microscope to use it.

4 FUNCTIONS OF THE MICROSCOPE

The stand consists of observing head, base and table. You

can grip the microscope by the lifting handle during transport.

Binocular or trinocular head

The head with the tubes can be rotated through 360° and is

equipped with two WF10x wide-field eyepieces. These are

marked with a spectacle symbol, because they are eyepieces

for spectacle wearers. This enables spectacle wearers with

glasses to perform microscopy without removing the specta-

cles.

Revolving nosepiece

The nosepiece is equipped with 4 achromatic objectives as

follows:

4x NA 0.13

10x NA 0.30

S40x NA 0.70

S100x NA 1.25 (oil)

The objectives 40x, and 100x are equipped with springs (S)

to prevent damage to the front lens and the microscope slide

and cover glass).

The numerical aperture (N.A.) of the objective indicates the

resolving power of the objective.

Fig. 2 MIC-231

Fig. 1 MIC-222

The total magnification can be easily calculated by multiply-

ing the magnification of the eyepiece by that of the respective

objective, as shown in the table:

Ocular

Objective

Magnification

10x

40x

10x

100x

10x

40x

400x

10x

100x

1000x

In MIC-200 series microscopes, the objectives are already

attached to the nosepiece.

Check that all the objectives are firmly attached. To do this,

grasp the knurled rings of the objectives with your fingers and

turn them to the right. Do not use a tool to tighten them. The

entire nosepiece with the 4 objectives can be turned by hand.

When you rotate the nosepiece, you will notice that the objec-

tives click into place. They do so in the correct position rela-

tive to the optical axis of the microscope.

Object table with mechanical stage

The microscope slide is placed in the specimen holder of the

mechanical stage and can be moved in X and Y direction.

The stage has a working area of 141 x 131 mm, the travel

range is 78 x 53 mm.

Coarse and fine adjustment

The focusing mechanisms for coarse and fine adjustment are

located on one axis (coaxial). On the fine adjustment knobs

there is a graduation. This can be used to measure the depth

of a specimen.

When the stage automatically slides down after using the mi-

croscope for some time, turn the adjustment ring on the in-

side of the coarse and fine adjustment knobs slightly in the

direction of the arrow. The coarse adjustment knob is tight-

ened to prevent the stage from sliding down.

Abbe condenser with iris diaphragm

Below the object stage is an Abbe condenser N.A. 1.25. The

condenser can be adjusted in height by turning. This allows

light to be focused on the specimen for contrast enhance-

ment. The condenser is already pre-centered.

The iris diaphragm with filter holder is located under the con-

denser. The light intensity can be adjusted by changing the

movable opening.

Illumination

The power-saving illumination is the reason why the micro-

scopes of the MIC-222 model do not need to be connected to

the mains all the time for operation, but can also be operated

independently of the mains, as they contain a rechargeable

Ni-MH battery. The illumination is a modern LED lamp with

the following specifications:

LED: 3W, brightness adjustable

Power supply: 100V-230V

C-mount adapter for the trinocular version MIC-231

A C-mount adapter is part of the scope of delivery of the MIC-

231 so that you can use each microscope camera which has

a C-mount thread.

5 WORKING WITH THE MICROSCOPE

Please follow the following instructions to achieve the best

results:

Adjustment of interpupillary distance and diopter dis-

tance

1. Adjusting the interpupillary distance

While looking through the eyepieces, adjust them until the left

and right fields of view coincide completely.

2. Adjusting the diopter

a) While looking through the right eyepiece, rotate the

coarse/fine focusing adjustment knobs to bring the specimen

into focus.

b) Look through the left eyepiece and rotate only the diopter

adjustment ring on the left eyepiece sleeve to bring the spec-

imen into focus.

Adjusting the lighting

For optimal contrast and resolution, please follow these

steps:

Place a micro slide on the stage and focus with the 4x

lens. The iris diaphragm is open.

Turn the condenser to the highest position.

Close the iris diaphragm until it is just visible at the edge

of the image field.

The microscope is now optimized for use with the 4x ob-

jective. Repeat this procedure with each additional magni-

fication to achieve the best balance between contrast and

resolution.

Attention: the maximum light intensity of the microscope

can damage your eyes if you use it with the 4x and 10x

objectives!

If you swap micro slides, start again with the 4x objective.

It is recommended to use slides of 1.0 to 1.2 mm thickness in

combination with cover glasses of 0.13 mm or 0.17 mm

thickness.

Battery

The MIC-222 microscope contains a 3.6V Ni-MH battery

pack.

A charging indicator is located on the side of the microscope.

It is red when connected to the power supply and green when

the battery is full. If the red indicator flickers, the battery is not

properly connected to the circuit board.

The microscope's illumination can be powered by a battery

pack for 4 to 8 hours without an external power supply.

Using the S100x oil immersion objective

Please follow the following recommendations for using S100x

N.A. 1.25 oil immersion lens:

Focus the image with the S40x lens.

Rotate the revolving nosepiece until the S100x objective

almost (not quite) clicks into place.

Drop a small drop of immersion oil (not part of the scope

of delivery, please order separately) on the center of the

specimen.

Then let the S100x objective snap into place.

The front lens is now in contact with the immersion oil.

View the specimen through the eyepiece and focus the

image with the fine adjustment knob.

The distance between objective lens and specimen is on-

ly 0.24mm!

If small bubbles are visible, turn the S100x objective a

few times to the left and right so that the front lens of the

objective moves in the oil. This will make the bubbles

disappear.

Then turn the stage with the fine adjustment knob down-

wards until the front lens does not touch the oil anymore.

Always clean the front lens of the S100x lens with a piece

of lens paper moistened with a drop of xylene or alcohol.

Now also clean the micro slide.

The S100x objective can also be used without immersion oil,

i.e. dry. However, please note that in this case the resolution

is much lower. Water may provide somewhat better resolu-

tion than using it dry.

Caution!

Never drop the xylene or alcohol directly onto the ob-

jective lens. This could cause xylene or alcohol to en-

ter the lens and dissolve the lens fixation!

Avoid oil contact with the other lenses!

6 MAINTENANCE AND CLEANING

Always cover the microscope with a dust cover after use. Al-

ways leave the eyepieces and objective lenses mounted so

that no dust can enter the inside parts of the microscope.

Cleaning the optical components

If the eyepiece lenses or the front lenses of the objectives are

dirty, you can clean them by wiping a piece of lens paper

over the lens surface in circular motions. If this does not help,

use a drop of xylene or alcohol on the lens paper. Never put

xylene or alcohol directly on the lens!

If dirt is clearly visible in the field of view, it is on the lowest

lens of the eyepiece. Remove the eyepiece from the tube and

clean the outside of the lens.

If dust is still visible, check whether the dust is in the eye-

piece by rotating it. If so, gently remove the bottom lens from

the eyepiece and clean it.

It is not necessary - and not recommended - to clean the lens

surfaces inside the objectives. Sometimes the dust can be

removed by high-pressure treatment with air. Otherwise,

there will be no dust inside the objectives until the objectives

are removed from the revolving nosepiece.

The most important step to a long lasting clean microscope

optics is to use the dust cover when you are not using your

microscope!

Caution! Cleaning cloths containing plastic fibers can

damage the coating of the lenses!

Maintenance of the stand and mechanical components

Simply remove dust with a brush. If the stand is very dirty, the

surface can be treated with a careful cleaning agent.

All moving parts such as the adjustment of the mechanical or

the coarse and fine focusing mechanism contain ball bear-

ings which are not sensitive to dust. The bearings can be

relubricated with sewing machine oil if necessary.

Change the lamp and the fuse

Switch off the power and pull the mains plug.

Now loosen the lower cover screw of the chassis and re-

move the cover to replace the bulb and fuse according to

the requirements of the different models.

After replacing the bulb and fuse, the cover should be re-

placed and the screws tightened.

7 TECHNICAL DATA

Infinity optical system

Siedentopf viewing head, tubes: 30° inclined (binocu-

lar/trinocular), 360° rotatable

WF10x/18mm eyepieces for spectacle wearers

Eyepieces of the monocular models secured against re-

moval

Quintuple nosepiece with click-stop

Achromatic objectives 4x NA 0.13, 10x NA 0.30, S40x NA

0.70, and S100x NA 1.25 (oil)

Condenser: Abbe N.A. 1.25 with iris diaphragm

Models with mechanical stage: area 141 x 131 mm, travel

range 78 x 53 mm

Coaxial coarse and fine focusing mechanism with gradua-

tions on the fine focusing mechanism

Lighting: 3 W LED (colour temperature 5000 K), adjusta-

ble

Integrated power supply unit 100...240 V, 50/60 Hz, 3W

Fuse: F3.15A/250V

Includes dust cover

Dimensions (H x W x L): 39 cm x 23 cm x 28 cm

Weight: 8.5 kg (MIC-222); 8.6 kg (MIC-231)

8 WASTE DISPOSAL

The packaging consists predominately of environmentally

compatible materials because they can be recycled and

should be passed on for disposal by the local recycling ser-

vice.

Should you no longer require this product,

do not dispose of it with the household re-

fuse.

Please return it to the address below for

proper waste disposal.

PHYWE Systeme GmbH & Co. KG

Service Department (Customer Service)

Robert-Bosch-Breite 10

37079 Göttingen / Germany

Phone +49 (0) 551 604-274

Fax +49 (0) 551 604-246

Mail [email protected], [email protected]

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