Sequoia LISST-ABS Manuale utente

LISST-ABS

Acoustic Backscatter Sensor

User’s Manual

Version 1.4

February 2017

Sequoia Scientific, Inc.

2700 Richards Road Suite 107

Bellevue, WA, 98005, USA

Phone: +1 425-641-0944; Fax: +1 425-643-0595

[email protected]; www.SequoiaSci.com

This page intentionally left blank

FOR TECHNICAL ASSISTANCE please contact your local

Distributor, or Sequoia if the instrument was purchased directly

from Sequoia. Please be sure to include the instrument serial

number with any correspondence.

A list of local Sequoia distributors can be found at our website

http://www.sequoiasci.com/about/contact/distributors

Sequoia Scientific, Inc. can be reached at

[email protected] or by phone +1-425-641-0944.

Contents

SECTION 1: QUICK START ...........................................................................................................1

SECTION 2: INSTRUCTIONS.........................................................................................................3

2.1 INTRODUCTION.........................................................................................................................3

2.2 SDI-12 OPERATIONS................................................................................................................4

2.3 RS232 OUTPUT OPERATIONS.................................................................................................10

2.4 ANALOG OUTPUT OPERATIONS...............................................................................................15

2.5 USB OPERATIONS VIA PC SOFTWARE.....................................................................................17

2.6 FIELD DEPLOYMENT SUGGESTIONS.........................................................................................27

APPENDIX A: LISST-ABS CABLE WIRING AND CONNECTOR PINOUTS..............................28

APPENDIX B: CONVERTING UNCALIBRATED CONCENTRATION TO CONCENTRATION 30

APPENDIX C: HOW THE LISST-ABS WORKS...........................................................................31

WARRANTY ..................................................................................................................................34

Section 1: Quick Start

Intro

Step 1:

Unpack box

Step 2: Install

Software

Step 3:

Plug in USB

cable and

Start Program

Thank you for purchasing Sequoia's LISST-ABS acoustic sediment sensor. This

Quick Start guide is intended to get you up running as quickly as possible.

Detailed instructions can be found in other sections of the manual.

The LISST-ABS uses acoustic backscatter to measure the concentration of

particles suspended in water. It outputs the Uncalibrated Concentration (or Cu).

The Uncalibrated Concentration is converted to Concentration using standard

calibration procedures such as used for turbidity sensors. For more details see

Appendix B and Appendix C.

The LISST-ABS is shipped ready for use. It takes just 3 easy steps to start

seeing some real measurements.

Inside the box should be the following items: LISST-ABS with protective red cap

and locking cap on the connector, a 5 to 50 meter long cable with underwater

connector and locking sleeve on one end and an 8-pin plastic Molex connector

on the other end, USB cable with mating Molex connector and three short

pigtails with mating Molex connectors. The three cables can be used for

connecting to dataloggers with screw terminals. There are separate pigtails for

SDI-12, RS232, and Analog Output connections. Also included is a credit card

style USB memory card with software installation files.

The sensor head is covered with a protective red cap to prevent accidental

impact and damage of the acoustic transceiver (white disc). Be sure to remove

the protective cap before use. The instrument will not work with the cap left on.

The underwater connector is also covered with a protective cap.

A simple program that can be used for testing the LISST-ABS is included on the

USB memory card. To install the software on your computer run the LISST-

ABS_installer.exe program on the memory card. This will install the LISST-ABS

software. This software requires the .NET 4.0 Framework. Most computers will

have this framework already installed as it is used by many other programs. A

message will appear if the .NET 4.0 needs to be installed. You can install .NET

using the dotNetFx40_Client.exe program on the memory card.

The LISST-ABS is a sensor only and must be connected to a power source. We

have designed a USB cable to provide power and communicate with the

instrument via one connection to a PC. Connect the long cable to the LISST-

ABS, attached the USB extension cable and then plug in the USB cable into the

PC. You should see the green LED on the LISST-ABS start to blink. Every time

the LISST-ABS blinks data is being sent up the cable to the PC.

If the LISST-ABS was purchased without a cable you will need to provide power

to the LISST-ABS and connect the RS232 output to the PC to use the provided

software.

You can now start the LISST-ABS software to see the values in real time. You

can also use the software to log data. Below is a screen shot of the software

while data is being logged to text file. More details on the operation of the

software can be found in Section 2.5.

The Cal Factor is a calibration value that is used to convert Uncalibrated

Concentration (Cu) to Concentration in mg/L. The LISST-ABS is calibrated and

adjusted at the factory using 75 to 90 micron particles so that the Cal Factor for

these particles will always be 1. You may use this value for a quick

approximation of concentration, but the most accurate results require your own

calibration, as described in Appendix B.

The LISST-ABS generates a new measurement every second. If you wish, you

can reduce sample-to-sample variability by averaging. Type the desired number

of measurements to average in the box below the plot. If you enter 5, for

example, each point on the plot will show the average of 5 samples, and the

update rate will slow to one point every 5 seconds.

To save measurements to a text file press the ‘Start Logging’ button You will be

asked to select a location and name for the file. Data will be saved to the file

until ‘Stop Logging’is pressed. The log file contains a header with the Cal

Factor value, time zone, and the instrument serial number. Following the

header are three columns of data: date, time, and measured concentration

(mg/L). The Log file is a space delimited ASCII file that can be easily imported

into programs such as Excel.

Detailed instructions on connecting the LISST-ABS to your datalogger are

covered in separate sections of this manual. For SDI-12 operations see Section

2.2. For Serial/RS232 operations see Section 2.3. For Analog Output

operations see Section 2.4.

Section 2: Instructions

2.1 Introduction

Thank you for your purchase of Sequoia's LISST-ABS acoustic

backscatter sensor. The LISST-ABS is a single-point acoustic

sediment sensor, designed to provide higher quality data than

optical turbidity sensors. Its key advantages over optical

turbidity sensors are:

Superior response to particles of grain size >30 microns.

Relatively flat sensitivity to particles from 30-400 microns in

sizes.

Greater immunity to fouling.

Wide dynamic range of concentrations from 1mg/L to 30 g/L.

The LISST-ABS internally measures the Attenuation Corrected

Backscatter(ACB). The ACB value is converted to an

Uncalibrated Concentration (Cu) and then output. To convert the

Uncalibrated Concentration to sediment concentration a simple

multiplier is required. The multiplier, or Cal Factor, is

dependent on the specific type and size of sediment being

measured. When a user recalibrates an instrument, it is this

multiplier that is updated. For more detailed understanding of

the technology, please see Appendix C.

The LISST-ABS can output the Uncalibrated Concentration

using three different methods, SDI-12, RS232, and analog

output. All three of these outputs are available on the

underwater connector on the end of the LISST-ABS. The

output used is determined by how the instrument is connected

to a datalogger.

The LISST-ABS can be powered in two ways. For testing

purposes, a special USB cable is available. When using this

cable, power to the instrument is provided by the USB port.

When connecting to a datalogger you will need to supply a

voltage between 10-18VDC. The current drain at 12V is 100

ma when actively collecting data. If the supply voltage is too

low the LISST-ABS will output an Uncalibrated Concentration

value of -999.

When the LISST-ABS is powered up the green LED on the

endcap will start to blink at about once per second. This

indicates the instrument is powered up and operating normally.

When the LISST-ABS is actively sampling it will double blink

(two blinks in quick succession). When idle but still powered up

it will do a single blink once per second.

2.2 SDI-12 Operations

SDI-12 Connections

The LISST-ABS is compatible with SDI-12 protocol version 1.3.

This section is divided into two parts, Connecting to your

datalogger and SDI-12 command summary.

SDI-12 only requires three wires. Power, Ground and Data.

Appendix A includes detailed descriptions of the use of the

individual pins of the 8-pin connector on the LISST-ABS.

However, in most case you will be using a cable provided by

Sequoia for connecting to your SDI-12 compatible datalogger.

To make the connections easier we have provided different

cable terminations designed specifically for different connection

requirements. For example we provide a termination with only

the three individual wires that are labeled and can be easily

connected to the datalogger. The mating connector makes it

easy to remove the long cable without disconnecting the wires

from the datalogger. You can also easily switch between the

datalogger and the USB cable if needed.

The wires are labeled and color coded. Connect the Black wire

to Ground. Connect the Red wire to +12V. Connect the Green

wire to SDI-12 Data.

The LISST-ABS is shipped pre-configured with an SDI-12

instrument address of 0.

The available SDI-12 commands are described below.

Following the command descriptions are some suggested best

practices for using the LISST-ABS with SDI-12.

LISST-ABS SDI-12 Command Summary

Command Name

Command Code

Notes

Address Query

Request address of single sensor on bus

Acknowledge Active

Request response from sensor at

address a

Change Address

aAb!

Change address of sensor at address a

to b

Send Identification

aI!

Send ID string including SI address, SDI

version, Manufacturers ID, sensor model,

sensor Firmware version and device

serial number

Start Measurement

aM!, aMC!

aM1!, aMC1!

aM2!, aMC2!

aM3!, aMC3!

aM4!, aMC4!

Start a measurement, returns number of

seconds till sample is ready to be read.

Sends service request when

measurement is complete. An M

command returns a single 1 second

average. M1, M2, M3, and M4 return a

30, 60, 120, or 300 second average.

C following a command requests a CRC

code in the data return (D command).

Start Concurrent

Measurement

aC!, aCC!

aC1!, aCC1!

aC2!, aCC2!

aC3!, aCC3!

aC4!, aCC4!

Same as Start Measurement command

except does not send service request.

Send Data

aDb!

Requests output b(0-9) from Sensor a

Continuous Measurements

(Read)

aR!

Not supported

Start Verification

aV!

Not Supported

See the next page for details of SDI-12 commands.

LISST-ABS SDI-12 Command Details

Basic Command Set

Address Query

Syntax:

Description:

Request address of single sensor on bus. Note: sensor must be the only

sensor on the bus or multiple instruments will respond simultaneously.

Example:

Command: ?!

Response: 0 (responds with address of sensor, in this case 0)

Acknowledge Active

Syntax:

Description:

Request response from sensor at address a.

Example:

Command: 0! (request sensor at address 0 to confirm it is active)

Response: 0 (responds with address of sensor)

aAb!

Change Address

Syntax:

aAb!

Description:

Change address of sensor at address ato b

Example:

Command: 0A3! (request sensor at address 0 to change to address 3)

Response: 3 (responds with new address of sensor)

aI!

Send Identification

Syntax:

aI!

Description:

Send ID string including SI address, SDI version, Manufacturers ID,

sensor model, sensor Firmware version and device serial number.

Example:

Command: 3I! (request sensor at address 3 to send ID string

Response: 313SequoiaSABS001001123456

Where:

3 = Sensor address (1 character)

13 = SDI Version compatibility (2 characters)

SequoiaS = Manufacturers ID (8 characters)

ABS001 = Sensor Model (6 characters)

001 = Sensor Firmware (3 characters)

123456 = Sensor Serial Number (6 characters)

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