Thermo 3307 Istruzioni operative

Models:

3307/3308 and 3310/3311

Steri-Cult CO2Incubator

Controlled RH with Sterilization Cycle

Operating and Maintenance Manual

Manual No: 7013307 Rev. 3

Steri-Cult Incubator _______________________________________________________________________________

Model Size Voltage

3307 Medium 115

3308 Large 230

3310 Medium 115

3311 Large 230

If the incubator is not used in the manner speci-

fied in this operating manual, the protection pro-

vided by the equipment design may be impaired.

Read This Instruction Manual.

Failure to read, understand and follow the instructions in

this manual may result in damage to the unit, injury to operat-

ing personnel, and poor equipment performance.

CAUTION! All internal adjustments and maintenance must

be performed by qualified service personnel.

The material in this manual is for information purposes only. The

contents and the product it describes are subject to change with-

out notice. Thermo Electron Corporation makes no representa-

tions or warranties with respect to this manual. In no event shall

Thermo be held liable for any damages, direct or incidental, aris-

ing out of or related to the use of this manual.

MANUAL NUMBER 7013307

-- -- 4/11/05 Added heat/CO2information pertaining to door openings in Section 5.7 ccs

3 22767/IN-3242 3/9/05 Corrected RH sensor from 290154 to 290174, electrical schematics ccs

2 -- 10/21/04 Updated sterilization Checkpoints concerning stacked units (per D. Figel) ccs

-- 22434/IN-3211 8/25/04 Updated water quality description for water bottle ccs

1 21730/SI-9181 6/15/04 Schematic updates, alternate source for 250085 solenoid valve ccs

0 22150/IN-3177 2/17/04 Standard manual size ccs

-- 21951/IN-3150 12/19/03 Inner door switch only to P/N 360263 ccs

-- 21980/IN-3156 11/07/03 Added min/max load and analog output boards max external cable length ccs

- 21596/IN-3124 10/24/03 New temperature sensor thermistor 290185 (glass enclosed) ccs

REV ECR/ECN DATE DESCRIPTION By

Steri-Cult Incubator _________________________________________________________________________Warnings

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol.

Hot surface(s) present which may cause burns to unprotected skin or to materials which may be damaged by elevated

temperatures

√ Always use the proper protective equipment (clothing, gloves, goggles, etc.)

√ Always dissipate extreme cold or heat and wear protective clothing.

√ Always follow good hygiene practices.

√ Each individual is responsible for his or her own safety.

Steri-Cult Incubator ___________________________________________________________________________Service

iii

Model 3307/3308 _______________________________________________________________________________

Warranty Notes

Information You Should Know Before Requesting Warranty Service

•Locate the model and serial numbers. A serial tag is located on the unit itself and on

the operating manual shipped with the unit.

• For equipment service or maintenance, or with technical or special application inquiries,

contact Technical Services at 1-888-213-1790 or 1-740-373-4763 (USA and Canada).

Outside the USA, contact your local distributor.

Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory;

typically ±1°C for temperature, ±1% for gases, and ±5% for humidity. Our service person-

nel can provide precise calibrations as a billable service at your location. Calibration after

a warranty repair is covered under the warranty.

• Damage resulting from use of improper quality water, chemicals or cleaning agents

detrimental to equipment materials.

• Service calls for improper installation or operating instructions. Corrections to any of

the following are billable services:

1) electrical service connection

2) tubing connections

3) gas regulators

4) gas tanks

5) unit leveling

6) room ventilation

7) adverse ambient temperature fluctuations

8) any repair external to the unit

• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God,

or improper installation.

• Repairs to parts or systems resulting from unauthorized unit modifications.

• Any labor costs other than that specified during the parts and labor warranty

period, which may include additional warranty on CO2sensors, blower motors,

water jackets, etc.

Steri-Cult Incubator __________________________________________________________________Table of Contents

Table of Contents

Section 1 - Installation and Start-up . . . . . . . . . . . . . . .1 - 1

1.1 Incubator Components . . . . . . . . . . . . . . . . . . . . . . . . .1 - 1

1.2 Inventory Management System . . . . . . . . . . . . . . . . .1 - 1

1.3 Control Panel Keys, Displays and Indicators . . . . . . .1 - 1

1.4 Operation of the Keypad . . . . . . . . . . . . . . . . . . . . . . .1 - 2

1.5 Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 - 2

1.6 Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . .1 - 2

a. Choosing the Location . . . . . . . . . . . . . . . . . . . . . .1 - 2

b. Stacking the Incubators . . . . . . . . . . . . . . . . . . . . .1 - 2

c. Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . .1 - 3

d. Installing the Shelves . . . . . . . . . . . . . . . . . . . . . . .1 - 3

e. Installing the Access Port Filter Assembly . . . . . .1 - 4

f. Installing the HEPA Filter . . . . . . . . . . . . . . . . . . .1 - 4

g. Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . .1 - 4

h. Connecting the Unit to Electrical Power . . . . . . . .1 - 4

i. Filling the Humidity Water Bottle . . . . . . . . . . . . .1 - 5

j. Connecting the CO2Gas Supply . . . . . . . . . . . . . .1 - 5

k. Humidity and Loading the Chamber . . . . . . . . . . .1 - 6

Section 2 - Incubator Settings . . . . . . . . . . . . . . . . . . . . .2 - 1

2.1 Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1

a. Setting the Temperature Setpoint . . . . . . . . . . . . . .2 - 1

b. Setting the Overtemp Setpoint . . . . . . . . . . . . . . . .2 - 1

c. Setting the CO2Setpoint . . . . . . . . . . . . . . . . . . . .2 - 1

d. Setting the Relative Humidity (RH) Setpoint . . . . .2 - 1

Section 3 - Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1

3.1 Calibration Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1

a. Calibrating the Temperature . . . . . . . . . . . . . . . . .3 - 1

Temperature Stabilization Periods . . . . . . . . . . . . . . .3 - 1

b. Calibrating the Infrared CO2System . . . . . . . . . . .3 - 1

c. Calibrating Relative Humidity . . . . . . . . . . . . . . . .3 - 1

Section 4 - Configuration . . . . . . . . . . . . . . . . . . . . . . . .4 - 1

4.1 Configuration Mode . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1

a. Turning the Audible Alarm ON/OFF . . . . . . . . . . .4 - 1

b. New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1

c. Setting the REPLACE HEPA filter reminder . . . .4 - 1

d. Setting an Access Code . . . . . . . . . . . . . . . . . . . . .4 - 1

e. Setting a Low Temperature Alarm Limit . . . . . . . .4 - 2

f. Enabling Temperature Alarms to Trip Contacts . . .4 - 2

g. Setting a Low CO2Alarm Limit . . . . . . . . . . . . . .4 - 2

h. Setting a High CO2Alarm Limit . . . . . . . . . . . . . .4 - 2

i. Enabling CO2Alarms to Trip Contacts . . . . . . . . .4 - 2

j. Setting a Low RH Alarm Limit . . . . . . . . . . . . . . .4 - 2

k. Setting a High RH Alarm Limit . . . . . . . . . . . . . .4 - 3

l. Enabling RH Alarms to Trip Contacts . . . . . . . . . .4 - 3

m. Selecting a Primary Tank w/ Gas Guard Option .4 - 3

n. Disabling the Gas Guard System . . . . . . . . . . . . . .4 - 3

o. Setting a RS485 Communications Address . . . . . .4 - 3

Section 5 - Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 1

5.1 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 1

5.2 Temp Controller Failure TEMP CNTRL ERROR . .5 - 2

5.3 RH System Fault . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 2

5.4 Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 2

5.5 Gas Guard Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 2

5.6 Water Level Alarms . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 2

5.7 Doors Open . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 - 2

Section 6 - Troubleshooting . . . . . . . . . . . . . . . . . . . . . . .6 - 1

Section 7 - Routine Maintenance . . . . . . . . . . . . . . . . . .7 - 1

7.1 Cleaning the Incubator Interior . . . . . . . . . . . . . . . . . .7 - 1

7.2 Cleaning the Cabinet Exterior . . . . . . . . . . . . . . . . . .7 - 1

7.3 Cleaning the Humidity System . . . . . . . . . . . . . . . . . .7 - 2

7.4 HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . .7 - 2

Section 8 - Sterilization Cycle . . . . . . . . . . . . . . . . . . . . .8 - 1

8.1 Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . .8 - 3

Section 9 - Factory Options . . . . . . . . . . . . . . . . . . . . . . .9 - 1

9.1 Connections to External Equipment . . . . . . . . . . . . . .9 - 1

a. Connecting the Remote Alarm Contacts . . . . . . . .9 - 1

b. Connecting the RS485 Interface . . . . . . . . . . . . . .9 - 1

c. Connecting the Analog Output Boards . . . . . . . . .9 - 1

9.2 CO2Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 - 2

a. Connecting the CO2Gas Supplies . . . . . . . . . . . . .9 - 2

b. De-activating the Gas Guard . . . . . . . . . . . . . . . . .9 - 2

c. Operation of the CO2Gas Guard . . . . . . . . . . . . . .9 - 3

9.3 Datalogger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 - 3

Section 10 - Specifications* . . . . . . . . . . . . . . . . . . . . . . .10-1

Section 11 - Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

11.1 Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

11.2 Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . .11-2

11.3 Replacing the CO2and RH Air Filters . . . . . . . . . . . .11-2

Appendix A - Class 100 Air

Declarations of Conformity

Section 1 - Installation and Start-up

1.1 Incubator Components

Figure 1-1

Steri-Cult Incubator

• Chamber Gas Sample Port - Used for sampling chamber

CO2content using a FYRITE or similar instrument.

• Power On/Off Switch - Mains disconnect

• Control Panel - Keypad, Displays & Indicators (Figure 1-2)

• Fold-down Storage Area - Store manuals, markers, etc.

• Leveling Feet - Used to level the unit

Note: The incubators are stackable. See Section 1.5.b.

1.2 Inventory Management System

Included in the parts bag

shipped with each unit is an invento-

ry management system. This system

consists of:

• A steel plate

• Five different colored magnets

• Five heat-resistant shelf labels

• Dry-Erase marker

Hang the plate on the inside of the outer door, using the

keyholes and hardware already installed on the door. Attach a

colored shelf label to the front edge of each shelf. The magnets

may be aligned on the plate, in order according to shelf label

color. Use the marker to record information about the product

on corresponding shelves. The magnets can be erased as needed

and rewritten.

The inventory management system magnet plate will not

withstand the heat of the sterilization cycle. Simply unhook the

whole plate from the outer door and set aside before initiating

the cycle, then reinstall afterward.

1.3 Control Panel Keys, Displays and Indicators

Figure 1-2

1. Silence - Silences the audible alarm.

2. Alarm Indicator - Light pulses on/off during an alarm

condition in the cabinet.

3. Mode Select Switch - Used to select Run, Setpoints,

Calibration and System Configuration Modes.

4. Message Center - Displays system status.

5. Mode Select Indicators -

Run: Run Menu

Settings: Set Points Menu

Calibrate: Calibration Menu

Configuration: Configuration Menu

6. Up and Down Arrows - Increments number values, tog-

gles between choices.

7. Enter - Stores the value into computer memory.

8. Heat Indicator - Lights when power is applied to the

heaters.

9. Heat Display - Displays temperature (°C)

10. Humidity Indicator - Lights when humidity is required

11. RH Display - Displays humidity (%)

12. Left and Right Arrows - Moves the operator through the

choices of the selected mode.

13. Inject Indicator - Lights when CO2is being injected into

the incubator chamber.

14. CO2Display - Displays CO2(%)

15. Sterilization Button - Starts Sterilization Cycle

Steri-Cult Incubator ____________________________________________________________Installation and Start-Up

1 - 1

1.4 Operation of the Keypad

The Steri-Cult incubator has four basic modes which allow

incubator setup: Run, Settings, Calibrate and System

Configuration.

Run is the default mode which the incubator will normally

be in during operation.

Settings is used to enter system setpoints for incubator

operation.

Calibrate is used to calibrate various system parameters.

Configuration allows for custom setup of various options.

The chart below shows the selections under each of the

Modes. Base Unit Displays are in bold type and Option

Displays are in italics.

Right and Left Arrows: Steps the operator through the

parameters of Settings, Calibrate and Configuration

Modes. The right arrow goes to the next parameter, the

left arrow returns to the previous parameter.

Up Arrow: Increases or toggles the parameter value that

has been selected in the Settings, Calibrate, and

Configuration Modes.

Enter: Must press Enter key to save to memory all

changed values.

Down Arrow: Decreases or toggles the parameter values

that have been selected in the Settings, Calibrate and

Configuration Modes.

Silence Key: Press to silence the audible alarm. See

Section 5 for alarm ringback times.

1.5 Displays

Message Center: Displays the system status (Mode) at all

times. Displays CLASS 100, SYSTEM OK or RH

WAITS ON TEMP (see Setting the RH Setpoint for fur-

ther details) during normal operation. Alarm messages

display if the system detects an alarm condition. See

Section 5, Alarms. The display message CLASS 100 is

a timing mechanism indicating that, under normal oper-

ating conditions with the HEPA filter installed, the air

inside the chamber meets the Class 100 air cleanliness

standard for particulates of 0.5 micron size or larger per

cubic foot of air. (For further information on the Class

100 classification of air quality, see Appendix A.)

3 Upper Displays: The first upper display shows the tem-

perature. The second display shows the percentage of

CO2. The third display shows the humidity percentage.

1.6 Installing the Incubator

a. Choosing the Location

Single and stacked units must be installed against a

wall or similar structure.

1. Maintain a three-inch clearance behind the incubator for

electrical and gas hook-ups. In addition, a three-inch

ventilation space is needed on each side of the unit.

2. Locate the unit on a firm level surface capable of sup-

porting the unit’s weight (340 lbs.-Models 3307 & 3308,

410 lbs.-Models 3310 & 3311)

3. Locate the unit away from doors and windows and heat-

ing and air conditioning ducts.

4. Lift the unit only by the sides of the cabinet base. Do

not attempt to lift it by the front and back. This places

stress on the outer door hinges.

b. Stacking the Incubators

With incubators in a stacked configuration, do not

leave both exterior doors open at the same time.

If the units have been in operation, turn them

both off and disconnect the power before begin-

ning any service work.

Steri-Cult Incubator ____________________________________________________________Installation and Start-Up

1 - 2

Run

Settings

Calibrate

Configuration

Default

Temperature

Temp Cal

Audible

Overtemp

CO2Cal

New HEPA Timer

CO2

RH Cal

Replace HEPA Reminder

Access Code

Temp Lo Alarm

Temp Relay

CO2Lo Alarm

CO2Hi Alarm

CO2Relay

RH Lo Alarm

RH Hi Alarm

RH Relay

Tank Select

Gas Guard

RS485 Address

Four stacking brackets (one shown at left) are

included in the parts bag shipped with each incu-

bator.

Figure 1-3

1. Unscrew and remove the 4 leveling feet from the unit to

be stacked on top and lift it onto the base unit. Align all

sides.

This incubator weighs 340 lbs. for Models 3307 &

3308, 410 lbs. for Models 3310 & 3311. Have suf-

ficient personnel available when lifting. Lift the

unit by the sides of the cabinet base to avoid plac-

ing stress on the outer door hinge.

2. Remove the hole plugs from the stacking bracket holes

(see Figure 1-5).

3. Align the holes in the brackets

with the mounting holes on the

sides of the top and bottom incu-

bator. Secure the two brackets

with the stainless steel screws

and washers provided in the

parts bag. See Figure 1-4.

4. The stacked incubators are ready for service.

c. Preliminary Cleaning

1. Using a suitable laboratory disinfectant, thoroughly

clean all interior surfaces.

d. Installing the Shelves

1. Install the pilasters, two on each side, with the tab facing

into the center of the chamber with their slots up. Press

downward on the top section of each pilaster to locate,

then release when in position (Figure 1-8). Fit each

pilaster securely into the indentations at each side of the

ceiling and the floor of the chamber. Figure 1-6 shows

the diffuser pan, pilasters, and shelf channels.

Note that there is a difference between the left and right shelf

channel. The channels are stamped as to which end goes to the

front of the unit.

2. Install the diffuser pan in the bottom of the unit with the

flanges down. See Section 9 at this point if your unit

includes a factory-installed datalogger (P/N 201912).

3. Install the shelf channels by placing the channel’s rear

slot over the appropriate rear tab on the pilaster. Pull the

shelf channel forward and engage the channel’s front

slot into the pilaster’s appropriate forward tab. Refer to

Figure 1-7.

Steri-Cult Incubator ____________________________________________________________Installation and Start-Up

1 - 3

Figure 1-7

Figure 1-6

Figure 1-4

Door

Sides of stacked units,

brackets installed Back of stacked units,

brackets installed

Figure 1-5

4. Figure 1-8 shows a shelf channel

installed on pilasters on the right

side of the chamber.

5. Slide the shelves in, side flanges

upward, along the channels into the

chamber.

Note: A Soft-Stop indicates the pullout limit

to prevent shelf tipping when fully extend-

ed.

e. Installing the Access Port Filter Assembly

1. Locate the opening in the top left corner on the inside of

the chamber. Remove the tape covering the opening on

the outside of the unit.

2. Install the the stopper assembly into the opening inside

the chamber. See Figure 1-9. Make sure the arrow on the

filter is pointing to the back wall of the chamber.

Filter MUST be installed inside the chamber or it

will become plugged with condensation and inhibit

the recovery of the RH system. Do not replace this

filter with a solid cover of any kind.

f. Installing the HEPA Filter

Use caution when handling the filter. The media

can be damaged if it is mishandled.

To avoid damage to the incubator, do not operate

the unit without the HEPA filter in place.

1. Remove the filter from the shipping box.

2. Remove the plastic covering from the filter, using cau-

tion not to touch the filter media.

3. Loosen black wingnuts in front corners of the chamber

ceiling (Figure 1-10/1-11).

4. Locate the metal lip at the upper back of the chamber.

Hook the HEPA filter carefully over the back edge of the

metal lip.

5. Lift the front of the filter to the ceiling of the chamber.

Push filter bracket back over the filter frame. Tighten the

wingnuts.

6. Refer to Section 6.4 for HEPA filter maintenance.

g. Leveling the Unit

Check for level by placing a bubble-style level on one of

the shelves. Turn the hex nut on the leveling feet counterclock-

wise to lengthen the leg or clockwise to shorten it. Level the

unit front-to-back and left-to-right.

h. Connecting the Unit to Electrical Power

See the serial tag on the side of the unit for electrical speci-

fications or refer to the electrical schematics in this manual.

The serial tag amp rating is based on the amper-

age draw during the sterilization cycle. Normal

operating amperage is much less. Ensure that the

electrical circuit will handle the amp draw of the

sterilization cycle.

Steri-Cult Incubator ____________________________________________________________Installation and Start-Up

Figure 1-8

orientation for

3307/3308

Figure 1-10

1 - 4

orientation for

3310/3311

Access port

filter assembly

Figure 1-9

Figure 1-11

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