THORLABS CM401 Manuale utente

Hyperspectral Imaging System

CM401

User Guide

Information in this document is subject to change without notice. The software described in this document is furnished under a license agreement or

nondisclosure agreement. The software may be used or copied only in accordance with the terms of those agreements. No part of this publication may be

reproduced, stored in a retrieval system, or transmitted in any form or any means electronic or mechanical, including photocopying and recording for any

purpose other than the purchaser's personal use without the written permission of ThorLabs, Inc..

ThorLabs, Inc.

108 Powers Court

Sterling, VA 20166

703-651-1700

Chapter 1 Warning Symbols and Definitions 1

Chapter 2 Safety 2

Chapter 3 Description 3

3.1. Features at a Glance 3

3.2. Specifications Overview 4

Chapter 4 Getting Started 6

4.1. Unpacking and Inspection 6

4.2. Setting up the CM401 7

4.2.1. General Setup 7

4.2.2. System Installation 8

Chapter 5 Capturing an Image 11

Chapter 6 Maintaining the CM401 16

6.1. Cleaning 16

Chapter 7 Specifications 17

7.1. Microscope Body Specifications 17

7.2. Sample Holder Specifications 17

7.3. XY Translation Stage Specifications 17

7.4. Objective Specifications 18

7.5. Condenser Specifications 18

7.6. Tunable Bandpass Filter Specifications 19

7.7. Camera Port Specifications 20

7.8. Camera Specifications 20

7.9. Motorized Controller Specifications 21

7.10. Plasma Light Source Specifications 22

Chapter 8 Mechanical Drawing 24

Chapter 9 Regulatory 26

9.1. Waste Treatment is Your Own Responsibility 26

9.2. Ecological Background 26

Chapter 10 Thorlabs Worldwide Contacts 27

Table of Contents

CM401

Chapter 1 Warning Symbols and Definitions

Below is a list of warning symbols you may encounter in this manual or on your device.

Symbol Description

Direct Current

Alternating Current

Both Direct and Alternating Current

Earth Ground Terminal

Protective Conductor Terminal

Frame or Chassis Terminal

Equipotentiality

On (Supply)

Off (Supply)

In Position of a Bi-Stable Push Control

Out Position of a Bi-Stable Push Control

Caution: Risk of Electric Shock

Caution: Hot Surface

Caution: Risk of Danger

Caution: Laser Radiation

Caution: Spinning Blades May Cause Harm

Page 1 TTN131444-D02

CM401 Chapter 1: Warning Symbols and Definitions

Chapter 2 Safety

All statements regarding safety of operation and technical data in this user guide will only apply

when the unit is operated correctly. Refer to the relevant User Guide of the individual product for

safety instructions. Please read the following warnings and cautions carefully before operating the

device.

WARNING

DO NOT use the device for anything other than its intended use. If the device is used in a

manner not specified by Thorlabs, the protection provided by the equipment may be

impaired.

WARRANTYWARNING

DO NOT open the controller, electronics enclosure, detector module, or computer. There are

no serviceable parts in these products. DONOTperform instrumentation maintenance or

service except as specifically stated. Any modification or servicing of this system by

unqualified personnel renders Thorlabs free of any liability. Only personnel authorized by

Thorlabs and trained in the maintenance of this equipment should remove any covers or

housings or attempt any repairs or adjustments.

CAUTION

The computer supplied with this system requires configuration for specific ACline voltages

of 110 VAC or 230 VAC nominal. Refer to the computer's User Guide for details.

WARNING

DONOT operate in a wet, damp, or explosive environment.

Rev A, June 27, 2017 Page 2

CM401 Chapter 2: Safety

Chapter 3 Description

Thorlabs' Hyperspectral Imaging System provides a platform to study a sample with co-located

features. In hyperspectral imaging, a stack of spectrally separated, two-dimensional images is

acquired. This allows quick sample identification and analysis.

The Hyperspectral Imaging system consists of Thorlabs' flexible Cerna®microscope, Kurios®

tunable filter, and scientific-grade camera. The tunable bandpass filter allows narrow spectral

regions to be individually imaged with a monochrome camera. It helps to acquire images with

quantitative spectral data for each pixel. Since the filter is not moved or exchanged during

measurement, the data is not subject to pixel shift image registration issues. In addition, Kurios filter

includes a benchtop controller with external triggers.

3.1. Features at a Glance

● General Microscope Features

♦Microscope body with 7.74" Throat Depth

♦10X Magnification Plan Fluor Objective

♦Nosepiece with 1" of Fine Z Travel

♦0.9 NA Condenser Suitable for Brightfield, Phase Contrast, Darkfield, and DIC (Differential

Interference Contrast)

♦Condenser Holder with 1" of Fine Z Travel

♦Two 3-Axis Controllers and Knob Boxes

● Sample Holder

♦Adjustable-Height Slide Holder

♦XY Translation Stage with 1" Motorized Travel

● Image Collection

♦Low-Noise 4 MP Scientific Camera

♦Monochrome CCD with High Quantum Efficiency

● Computer

♦High Performance Computer for Rapid Image Acquisition and Data Transfer

♦Automated Wavelength Switching and Image Capture using DAQ and ThorImage®LS

Software

● Illumination

♦High Power Plasma Light Source

♦Liquid Crystal Tunable Bandpass Filter for Vibrationless Wavelength Switching from 420 to

730 nm

Page 3 TTN131444-D02

CM401 Chapter 3: Description

3.2. Specifications Overview

The following table provides an overview of the CM401 specifications. For detailed specifications

on individual devices, see Specifications Chapter on Page 17.

Specification Value

Microscope Cerna Series Modular Microscope

Objective Nikon 10X Plan Fluorite Objective (N10X-PF)

Condenser 0.9 NAAir Condenser (CSC1002)

Tunable Filter Specifications

Item # KURIOS-VB1

Wavelength Range 420–730 nm

Bandwidth, CWL = 550 nma

Narrow: 10 nm FWHM

Medium: 21 nm FWHM

Wide: 55 nm FWHM

Minimum Incremental Step Size 1 nm

Tuning Accuracy ±FWHM/10

Switching Speedb

Narrow: <200 ms

Medium: <80 ms

Wide: <50 ms

Scientific Camera Specifications

Item# 4070M-GE

Sensor Type ONSemiconductor KAI-04070M Monochrome CCD

Number of Active Pixels 2048 x 2048 (Horizontal x Vertical)

Pixel Size 7.4 µm x 7.4 µm

Imaging Area 15.2 mm x 15.2 mm (Horizontal x Vertical)

Host PC Interface Gigabit Ethernet

Vertical Hardware BinningcContinuous Integer Values from 1 to 10

Horizontal Software BinningcContinuous Integer Values from 1 to 10

Exposure Time 0 to 1000 s in 1 ms Increments

CCD Size and BinningcOne Tap Two Taps

20 MHz 40 MHz 20 MHz 40 MHz

Full Sensor (2048 x 2048) 4.1 fps 7.7 fps 7.4 fps 13 fps

Full Sensor, Bin by 2 (1024 x 1024) 7.5 fps 13.1 fps 12.8 fps 20.5 fps

Full Sensor, Bin by 10 (204 x 204) 21.6 fps 30.5 fps 30.0 fps 38.1 fps

Rev A, June 27, 2017 Page 4

CM401 Chapter 3: Description

Specification Value

Light Source Specifications

Item# HPLS343

Type High-Power Plasma Light Source and Ø3 mm LLG (Liquid

Light Guide)

Wavelength Range 350–800 nm

Optical Output Powerd4.0 W (Typical) 3.5 W (Minimum)

Automated Stage Specifications

1" (25.4 mm) Motorized Travel Nosepiece and Condenser Holder

1" (25.4 mm) Motorized Travel Sample Holder

Bidirectional Repeatability 1µm

Backlash 1µm

Minimum Achievable Incremental

Movement 100 nm

Minimum Repeatable Incremental

Movement 200 nm

Included Software

ThorImageLS 3.1 with Lifetime Support

a. The bandpass width increases linearly with wavelength.

b. The switching speed depends upon the bandpass width setting and the initial and final

wavelength.

c. Camera Frame Rate is impacted by the Vertical Hardware Binning parameter.

d. Measured at the output of the liquid light guide, when bulb and LLG are at start-of-life.

Page 5 TTN131444-D02

CM401 Chapter 3: Description

Chapter 4 Getting Started

This section is provided for those interested in getting the Hyperspectral Imaging System up and

running quickly.

4.1. Unpacking and Inspection

Open the package, and carefully remove the CM401 and its accessories. The table lists the

standard accessories shipped with the device.

Name Quantity

Microscope Body 1

Monochrome Scientific Camera (4070M-GE) 1

I/O Breakout Board for Scientific Camera (TSI-IOBOB) 1

Computer with Monitor, Keyboard, and Mouse 1

3 Axis Controller with Knob Box (MCM3001) 2

High Power Light Source with Liquid Light Guide

(HPLS343) 1

KURIOS Filter Controller with Optical Head Cable

(KURIOS) 1

10X Nikon Plan Fluor Objective (N10X-PF) 1

Nikon D-CUD Universal Condenser 0.90 NA

(CSC1002) 1

Rigid Stand on PLS-XY Translation Stage 1

BNC to SMA Cable 2

Auxiliary Cable (8050-CAB1) 2

M6 Socket Head Cap Screw 16

1/4-20x1/2" Socket Head Cap Screw 14

M6 Flat Washer 10

ThorCam Camera Software CD 1

Thorlabs Digital Camera Quick Start Guide 1

Inspect the device and its accessories for any missing parts or damage. If there is any problem,

please contact our nearest office (see Thorlabs Worldwide Contacts Chapter on Page 27 for details).

Rev A, June 27, 2017 Page 6

CM401 Chapter 4: Getting Started

4.2. Setting up the CM401

4.2.1. General Setup

For optimal image acquisition, we strongly advise that the following standard practices are

met:

● Acquire images using actively isolated or anti-vibrational table platforms. Failure to

utilize appropriate platforms results in poor quality images. Thorlabs offers a wide

array of optical tables and platforms that provide the stability for your imaging

needs.

● Whenever appropriate, avoid the placement of other instruments or machines that

vibrate in close proximity to your imaging setup. Such items include refrigeration

units, cell culture incubators, hoods, etc.

● Minimize air drafts as it can result in movement of your imaging system. This can

result in problems related to focusing on your sample. Furthermore, drafts can also

lead to particulate contamination that can damage your hardware and/or introduce

artifacts into your imaging field.

● Operate in light-controlled environments to make sure of best quality images.

● Make sure that your hardware setup is operated within the appropriate

environmental settings according to the manufacturer's specifications. Such

considerations include enough space from surrounding walls and ceilings from

your hardware, sufficient sources of electrical power, and proper regulation of

temperature and humidity, etc.

Page 7 TTN131444-D02

CM401 Chapter 4: Getting Started

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