WPI MMP Manuale utente
WORLD
PRECISION
INSTRUMENTS
MMP/DMP
Manual Microsyringe Pumps
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
090821
MMP/DMP
World Precision Instruments i
Copyright © 2021 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 2
Parts List...................................................................................................................................... 2
Unpacking................................................................................................................................... 3
OPERATING INSTRUCTIONS......................................................................................................... 4
Injection Fluid Filling................................................................................................................. 4
Improving Performance .......................................................................................................... 5
Shortening the Teon Tubing........................................................................................... 6
Removing Air from the Pipette Coupling Tube ............................................................ 6
Removing the Stopcock ..................................................................................................... 6
Determination of Total Volume Pumped............................................................................ 7
ACCESSORIES................................................................................................................................... 8
Syringes for MMP/DMP ........................................................................................................... 8
Replacement Parts ................................................................................................................... 8
SPECIFICATIONS.............................................................................................................................. 9
DECLARATION OF CONFORMITY ..............................................................................................10
WARRANTY .....................................................................................................................................11
Claims and Returns ................................................................................................................11
Repairs.......................................................................................................................................11
ii World Precision Instruments
MMP/DMP
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Male Luer
Connector
Teflon Tubing
Male Luer
Connector
Syringe Mount Bracket
Syringe Piston
Mount Bracket Micrometer
Adjustable
Micrometer Mount
Pipette
Pipette Holder
Non-Rotating
Micrometer Spindle
Syringe Mount
Side Female Luer
Connector with
Syringe Attached
Fig. 1—MMP Manual Microsyringe Pump is shown fully assembled.
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Fig. 2—On the DMP the micrometer has a digital display
INTRODUCTION
The MMP is a convenient tool for precise manual injection of uid using glass pipettes
or similar injection devices. DMP has a digital micrometer head for increased precision
and ease of use. The design allows visual feedback of ow at the pipette tip and can
also be used as a manual micro syringe pump for perfusion or withdrawal of liquids.
The resolution of the injection volume can be continuously varied from 10nL to the
microliter range, depending on the syringe volume used. Either oil or air can be used
as the transfer media to assist in the injection of uid.
The non-rotating micrometer head has a resolution of 10µm per division and
advances 500µm per full revolution. The entire frame body of the injector is
constructed with polished stainless steel for stability and durability. The piston of the
micrometer can be slid across the rail to the syringe’s plunger position. Small diameter
PTFE tubing is used to improve the accuracy and solution compatibility. The unique
design of the pipette holder can securely hold any pipette with an outer diameter of
between 1.0mm and 1.5mm. The necessary accessories for removing air and lling the
syringe and tubing with liquid are included. The system comes complete with a 100µL
gas tight syringe. However, other syringe sizes are available on request.
Parts List
After unpacking, verify that there is no visible damage to the sensor. Verify that all
items are included:
(1) Stainless steel stand with micrometer
(1) 100µL gas-tight SGE syringe (#SGE100TLL)
(1) 4-way stopcock (#14057-10)
(1) 10mL plastic syringe (#3744-10)
(1) Injection assembly (pipette holder, Teon tubing, luer-to-tubing coupler, gasket for
1.0mm OD pipette)
(1) Extra gasket for 1.0mm OD pipette (Green Silicone)
(1) Extra gasket for 1.2mm OD pipette (Black Silicone)
(1) Extra gasket for 1.5mm OD pipette (Blue Silicone)
MMP/DMP
World Precision Instruments 3
(1) Extra gasket for 1.65mm OD pipette (Red Silicone)
(1) Digital Micrometer Head (model DMP only), packed separately to reduce risk
of damage during shipment. To install, slide it all the way onto the hole in the
adjustable micrometer mount, then attach the syringe piston mount bracket
as shown in Fig. 3. Tighten the screws on the side of the syringe bracket and
micrometer mount with the tool provided.
(1) Instruction Manual
Teflon Tubing
Male Luer Connector
4-way Stopcock
Side Female Luer Connector
Microsyringe
10mL Plastic Syringe
Fig. 3—Fluid injection components
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 11 of this manual. Please contact WPI Customer
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper or
plastic surrounded with at least 100mm (four inches) of shock absorbing material. For
further details, please read the section entitled “Claims and Returns” on page 11 of
this manual.
4 World Precision Instruments
OPERATING INSTRUCTIONS
Injection Fluid Filling
The MMP/DMP can be used with either oil
or air as the transfer medium. In general,
however, only 500µL or 1000µL syringes (not
included) are used with air, because of the
compressibility of air. Almost a micoliter may
have to be pumped before the pressure builds
up to the point where the response in the
pipette is as fast as with a liquid. Therefore,
an oil-based transfer medium such as silicone
oil, mineral oil or Fluorinert should be used, if
precision in the nanoliter range is desired.
Fig. 4—(Right) Male, female and side luers are open (MFS position)
The 10mL plastic syringe provided with the MMP/DMP is used to ll both the micro
syringe and the Teon tubing with the injection uid of your choice. The procedure
described in this section is designed to maximize the removal of air bubbles, which
would otherwise slow the response time of the MMP/DMP and decrease its precision
when dealing with very small injection volumes.
TIP: If it is desirable to have the microsyringe mounted during this process, the syringe
piston will be easier to move if the micrometer mount screw is loosened so that the
entire micrometer mount can be slid across the base of the MMP/DMP.
NOTE: Please read through all of the instructions before beginning.
1. Disconnect everything from the stopcock. Fill both syringes with the injection uid.
2. Attach the 10mL syringe to the side female luer connector of the stopcock.
3. Turn the knob of the stopcock to the position shown in Fig. 4 (MFS position). The
stopcock valve allows four dierent ow paths between the female luer, male luer,
and side female luer connectors (Figs. 4,6,7 and 8).
Fig. 4
Fig. 8
Fig. 7
Fig. 6
Valve Position Flow Path
Fig. 5—(Left) Flow positions.
Fig. 6—(Right) Female and side luers are open (FS position)
Male Luer
Connector
Side Female Luer
Connector
Female Luer
Connector
MMP/DMP
World Precision Instruments 5
Fig. 7—Female and side Luers are open (MS position)
Fig. 8—Male and female Luers are open (MF position)
For each step in the lling process, make sure the Luer tips are lled with the uid
before connecting them so that bubble formation is minimized.
4. Advance the piston of the large plastic syringe until the open ends of the stopcock
are at least partially full. This displaces trapped air within the valve of the stop-
cock. Then turn it clockwise to the position shown in Fig. 4 so that the male Luer
connector is blocked o (FS position) and attach the microsyringe.
If there are any bubbles between the micro syringe and stopcock they can be expelled
into the Teon tubing after turning the knob to the MF position (Fig. 8). Then, turn it
back to the FS position so that it can be relled with the 10mL syringe. This procedure
may have to be repeated with the smaller microsyringe sizes. Once a bubble free
connection is established with the micro syringe, put the stopcock knob in the MS
position (Fig. 7), attach the Teon tubing and pipette, and advance the piston of the
plastic syringe until all air is expelled.
Turn the knob to the MF position so that the micro syringe is connected exclusively
to the Teon tubing as shown in Fig. 8. If any bubbles enter the system around the
stopcock during use, expel them directly into the plastic 10mL syringe.
Improving Performance
For most cases, the precision obtained from following the steps in the previous
section is adequate. If more precision is required, it may be desirable to try the
following techniques and modications.
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CAUTION: Pipette holder and Luer-to-tubing couplers are very small and may
be easily misplaced.
Shortening the Teon Tubing
In general, the MMP will work better if the Teon tubing is as short as is convenient for
a given application. Although the tubing is very sti, it still may expand and contract
slightly from the pressure generated by the micro syringe. In addition, the higher
pressure needed to push the injection uid through a longer tube increases the
chance that air will slip through one of the seals. In order to shorten the tubing, one
of the ends must be exposed so that it can be cut. The easiest way to do this is to
unscrew the end cap of the Luer-to-tubing coupler (Fig. 9).
Female Luer
Connector
End Cap
Gasket
Teflon Tubing
Female/Female Adapter
Fig. 9—Luer to tubing coupler parts
Removing Air from the Pipette Coupling Tube
Since the pipette coupling tube must accommodate both the Teon tubing and a
range of pipette sizes, there will generally be enough dead volume outside the main
uid passage for air to get trapped in. If the pipette holder is taken apart (Fig. 10), a
bubble-free connection can be achieved when each end of the coupling tube is lled
with injection uid before it is re-assembled. As the ends of the Teon tubing and
pipette are slipped into the coupling tube, make sure they meet in the middle so that
dead volume is minimized and the risk of bubble formation is reduced.
Removing the Stopcock
The stopcock contains a lot of dead volume. The MMP/DMP can be used without it,
though this will make it more dicult to set up, and there is no easy way to remove
bubbles that form during use. However, it might improve performance slightly for
some applications.
Pipette Holder Body
GasketEnd Cap Gasket
Pipette Coupling Tube Teflon TubingGlass Pipette
Fig. 10—Pipette holder parts
First, load the Teon tubing directly with the 10mL syringe. Continue to eject uid
out of the syringe as it is being separated from the tubing so that the female Luer
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