Zeiss LSM 510 META Manuale utente

LSM 510 and LSM 510 META

Laser Scanning Microscopes

Operating Manual

Release 3.2

INTRODUCTION LSM 510

Carl Zeiss LSM 510 META

II B 45-0008 e 10/02

Knowledge of this manual is required for the operation of the instrument. Would you therefore please

make yourself familiar with the contents of this manual and pay special attention to hints concerning the

safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

communication of its contents are not permitted. Violations will entail an obligation to pay

compensation.

Developed in

Collaboration with the European Molecular Biology Laboratory (EMBL)

PF 102209

Meyerhofstr. 1

69012 Heidelberg

GERMANY

Phone: ++49-6221-387-0

Telefax: ++49-6221-387-306

Issued by Carl Zeiss

Advanced Imaging Microscopy

07740 Jena

GERMANY

Phone: ++49-3641 64-34 00

Telefax: ++49-3641 64-31 44

E-mail: micro@zeiss.de

Internet: www.zeiss.de/lsm

Number of this manual: B 45-0008 e

Date of issue: 10/2002

LSM 510 INTRODUCTION

LSM 510 META Carl Zeiss

B 45-0008 e 10/02 III

How to make best use of the LSM 510 operating instructions

A few symbols in these operating instructions will help you to recognize the nature and purpose of

information immediately:

The WARNING symbol warns against hazards for the user that might arise when operating the

laser.

This WARNING symbol warns against hazards from dangerously high voltages.

The CAUTION symbol warns against faults and hazards that might arise during operation and

which might cause damage to the unit.

☞The NOTE symbol will help you to optimally solve your work problem. It represents a practical

tip which will help you to find out which settings and methods are capable of improving or

accelerating a procedure.

The HOT SURFACE symbol warns against hazards for the user that might arise when touching

the lamp housing during operation.

The MAINS PLUG symbol remembers service personal to pull the mains plug before opening

the device housing.

Depending on the problem, these operating instructions will supply you with various possibilities:

• If you want to know where to find certain general areas of information, refer to the following outline

of sections to get a general overview.

• You will find a detailed table of contents at the start of every chapter. There you will see at a glance

what topics are dealt with in detail.

Always remember: The time you invest in getting acquainted with the product will pay

for itself many times over in your application task.

INTRODUCTION LSM 510

Carl Zeiss LSM 510 META

IV B 45-0008 e 10/02

Scope of Equipment Supplied

Country: ..............................................

Order number: ..............................................

Serial number: ..............................................

Delivery date: ..............................................

Custom configuration: ..............................................

Axioskop 2 FS mot 000000-1056-2308



Axioplan 2 imaging MOT (ie) 000000-1166-265



Axiovert 200 M SP 000000-1115-092



Axiovert 200 M BP 000000-1115-094



Axiovert 200 M BP/SP 000000-1115-289



Objectives: ..............................................

..............................................

Confocal Laser Scanning Module LSM 510

Configuration 1 000000-1219-975



Configuration 2 000000-1219-977



Configuration 3 000000-1219-978



Configuration 4 000000-1219-979



Configuration 5 000000-1219-980



Configuration 9 000000-1219-981



Configuration 11 000000-1219-982



Configuration 12 000000-1219-983



Configuration 13 000000-1219-984



Configuration 15 000000-1219-985



Configuration 16 000000-1219-986



Configuration 18 000000-1219-987



Configuration 3 META 000000-1219-995



Configuration 13 META 000000-1219-997



Configuration 15 META 000000-1220-000



Configuration 18 META 000000-1220-004



Control computer 000000-0438-360



21“ monitors 000000-1060-089



TFT monitors 000000-0435-035



The license to the LSM control software is included in each configuration 1...18 (META).

LSM 510 INTRODUCTION

LSM 510 META Carl Zeiss

B 45-0008 e 10/02 V

Optional software:

Image VisArt option 000000-1207-934



3D for LSM option 000000-1207-919



3D Deconvolution option 000000-1207-920



Physiology option 000000-1207-930



Topography option 000000-1207-933



Multiple Time Series option 000000-1207-926



Macro Reader and Editor option 000000-1207-925



StitchArt option 000000-1207-932



Canon S 830 D Photo printer 000000-0445-508



Kodak XLS 8650 PS printer 000000-1113-131



Transparency exposure device (D) 412410-9011-000



Large system table 453031-0000-000



Small system table 453032-0000-000



System baseplate 000000-1171-342



Active anti-vibration table 30" x 30" 000000-1177-841



Active anti-vibration table 1200 mm x 1400 mm 000000-1198-394



Active anti-vibration table 1800 mm x 1400 mm 000000-1150-353



UV laser 412410-9015-000



XY scanning stage for Axiovert 200 M BP 000000-1113-509



XY scanning stage for Axioplan 2 imaging MOT 000000-1027-823



Piezo objective drive W = 0.8" 000000-1210-045



High resolution Z stage HRZ 200 for Axiovert 200 M 000000-1115-248



High resolution Z stage HRZ 200 for Axioplan 2 imaging MOT (ie)000000-1115-245



Set of INDO filters 447960-0000-000



Set of SNARF filters 447961-0000-000



AxioCam HRm 000000-0445-553



AxioCam HRc 000000-0412-312



INTRODUCTION LSM 510

Carl Zeiss LSM 510 META

VI B 45-0008 e 10/02

The LSM 510 in the configuration as checked above

was installed and handed to the customer in functional condition

on ................................................

by ................................................

Phone: ................................................

Fax: ................................................

The customer has been instructed on how to operate and

maintain the equipment.

(Place)......................................................, (date) .....................................

...............................................................

Carl Zeiss Jena GmbH

Microscopy Division

...............................................................

Customer

One copy to be kept by customer

One copy to be kept by Carl Zeiss

LSM 510 INTRODUCTION

LSM 510 META Carl Zeiss

B 45-0008 e 10/02 VII

1 Notes on Device Safety

This section contains general notes on device safety, safe operation, and possible hazards

caused by failure to observe the instructions.

2 LSM 510 - Setup Requirements

The Setup Requirements section outlines the installation and supply requirements of the

LSM 510 Microscope System, together with the relevant specifications.

3 Introduction to Laser Scanning Microscopy

Here you will find an introduction to Laser Scanning Microscopy, with an explanation of the

principles of confocal imaging. The section also outlines the ways to present LSM image series

in three dimensions, and introduces you to the performance features of your LSM 510.

4 Quickstart

5 Operation in Expert Mode

In the Operation section you will find the most important steps and procedures of the

LSM menu structure. The step-by-step description how to get an image will be shown by

typical application examples including the WINDOWS NT 4.0 graphic user environment.

6 VBA Programming for LSM

7 Routine Mode and Tools

This section contains a description of the Routine Mode for scanning images using the

LSM scanning module and the use of the tools for setting the microscope.

8 3D for LSM 510

This section contains a description of the LSM 3D software package (basic program and add-

ons). At the same time, all functions and settings are presented in a systematic form and in

the order in which they can be reached from the basic menu via sub-menus and dialog boxes.

9 Annex

The annex contains the Application-specific Configurations and special notes and information

for using the LSM microscope.

10 Multiphoton Laser Scanning Microscopy - Using the Zeiss LSM 510 NLO

11 Certification

INTRODUCTION LSM 510

Carl Zeiss LSM 510 META

VIII B 45-0008 e 10/02

LSM 510 NOTES ON DEVICE SAFETY

LSM 510 META Contents Carl Zeiss

B 45-0008 e 10/02 1-1

CHAPTER 1 NOTES ON DEVICE SAFETY

CONTENTS

Page

1 NOTES ON DEVICE SAFETY...................................................................................................1-3

1.1 General...................................................................................................................................1-3

1.2 Regulations .............................................................................................................................1-3

1.3 Notes on Setting up the Microscope System.............................................................................1-4

1.4 Notes on Handling the Computer and Data Media ...................................................................1-5

1.5 Notes on Care, Maintenance and Service .................................................................................1-6

1.6 Notes on Handling the Laser Components ...............................................................................1-7

1.7 Warning and Information Labels..............................................................................................1-8

NOTES ON DEVICE SAFETY LSM 510

Carl Zeiss Contents LSM 510 META

1-2 B 45-0008 e 10/02

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