Accu-Scope EXC-120 SERIES Manuale utente

73 Mall Drive, Commack, NY 11725 ● PH (631) 864-1000 ● F (631) 543-8900 ● info@accu-scope.com ● www.accu-scope.com

EXC-120 Microscope Series

Phase Contrast Supplement

Alignment of the Phase Contrast Components

Underside View of Phase Turret,

Variable Aperture Diaphragm

and 4 Annuli Holders, Adjustable (X-Y)

Components

Phase contrast consists of two components for each phase

contrast objective installed on the microscope:

Phase Retarder Ring inside each Ph objective. This gray

Phase ring is observed using the focusable Phase Centering

Telescope supplied with the system. In the following

instruction you will be advised when to substitute the

telescope for one of the microscope’s eyepieces

Annulus Ring in the condenser. There is a different

annulus for each objective—one ring corresponding to each

Ph objective. Its purpose is to shape the incoming light into

a hollow cylinder. The annuli are mounted in holders that are

individually movable in an X-Y manner— so as to be able to

align the image of each annulus with the gray phase ring in

each objective.

Front Turret Installed and Positioned

Currently in Optic Axis

Installation

Install the Ph objectives on the microscope’s nosepiece

starting with the 10x, and then rotate the nosepiece clockwise

and install the 20x, 40x and 100xR oil objectives in numerical

order.

Install the Phase Contrast Condenser/Turret into the

condenser carrier mounting ring and tighten the set screw

with the screwdriver that came with your microscope.

NOTE: The condenser mounting ring has been pre-centered

at the factory.

Select the B for brightfield position on the Condenser/Turret

by rotating it clockwise until the B clicks into place as shown.

Rotate the nosepiece and bring the 10x objective into

position.

Raise the Condenser/Turret to its highest position.

Place a familiar “prepared, stained specimen” on the

stage and focus the 10x objective on this specimen.

10x Annulus

20x Annulus

40x Annulus

100x Annulus

73 Mall Drive, Commack, NY 11725 ● PH (631) 864-1000 ● F (631) 543-8900 ● info@accu-scope.com ● www.accu-scope.com

Centering Telescope Correctly Installed

Alignment

To align, you need to superimpose the image of each

annulus onto its corresponding phase ring in each

objective by using the Centering Telescope as described

in the sequence below.

Replace one eyepiece with the Centering Telescope:

Remove one of the eyepieces by gently pulling it out of

the eyetube.

Insert the Centering Telescope into the eyetube.

1. Looking through the Centering Telescope, focus the

telescope on the gray 10x Ph phase ring in the objective

until the gray ring is sharp.

2.With the 10x objective in position, select the annulus

position that matches the 10x objective in use by rotating

the Condenser/Turret clockwise until the 10x annulus

position is in front and clicks into place.

3. Move the 10x annulus on the underside of the

Condenser/Turret to align the image of the annulus to the

phase ring in the objective as it appears in the diagram

below as (c).

Repeat the above procedure 1-3 for each Phase

contrast objective.

When alignment of all objectives is completed, replace

the Centering Telescope with the eyepiece and retighten

the set screw – be careful not to overtighten the set

screw.

Misaligned

to the left

Misaligned

to the bottom

CORRECTLY

ALIGNED

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