Accu-Scope 3025 Series Manuale utente

Figure 1

3025 Series Turret Phase Condenser

(Bottom View)

Figure 2

Centering Telescope

Figure 3

Rotating

Annuli Wheel

Rotating

Annuli Wheel

Rotating Turret Wheel

3025 SERIES PHASE CONTRAST SET-UP INSTRUCTIONS

1. Place the microscope in front of you and plug in your light source.

2. Mount the objectives clockwise (lowest magnification to highest magnification) onto the

microscope nosepiece.

3. Install the turret phase condenser (Fig. 2) into the condenser holder located under the

stage and tighten into place.

4. Position the turret phase condenser so that the “BF” on the rotatable annuli selector wheel

is facing you. This places the condenser iris diaphragm in the light path. Rack up the

condenser to the top and open the diaphragm.

5. Place a specimen slide on the stage and focus on it using the 10X objective.

6. Shift the slide to an area where the specimen is no longer visible; however, do not change

the focus.

7. Rotate the phase turret wheel counter-clockwise until the “10” figure clicks into position.

Note: There are two rotating wheels located at the front of the phase condenser. They are

used to center the annuli in the condenser or phase rings.

8. Remove one of the eyepieces of the microscope and replace it with the phase telescope

(Fig. 3). Note: The upper collar of the phase telescope can be moved in or out allowing

you to bring the phase rings into focus.

9. Looking through the phase telescope you will see a dark field of view with two different

rings ( a & b), one bright (white) and the other black.

10. While viewing the rings through the phase telescope, adjust the two rotating wheels from

the turret phase condenser until the narrower white ring is superimposed equidistant

within the black ring (c).

11. Remove the phase telescope from the microscope and replace the eyepiece.

12. Bring the specimen back into the field of view and refocus the image until it is sharp. The

image will be detailed and exhibit fine structural information.

NOTE: You must re-center the phase rings every time you change magnification by using a

different objective. The turret wheel must be rotated so its number corresponds with the

objective being used. To return to brightfield viewing, rotate the turret wheel until it is at the

“BF” position.

TROUBLESHOOTING GUIDE

PHASE CONTRAST MICROSCOPY

PROBLEM

CAUSE

CORRECTIVE MEASURE

Poor phase

contrast

image

is obtained

The condenser phase

annular

diaphragm image and the objective

phase plate do not match.

Adjust the phase

annular

diaphragm so that it matches with

the objective phase plate.

The condenser phase annular

diaphragm and the objective phase

code do not match.

Insert the phase

nular

diaphragm with the same phase

code as the objective into the

optical path.

The field diaphragm image is not

focused on the specimen surface.

Move the condenser up or down to

focus the image on the specimen

surface.

The phase difference of the

specimen is too large.

Prepare the specimen using a

different sealant and specimen

thickness

The type of pha

objective does

not match the phase difference of

the specimen.

Select a phase objective suitable

for the specimen.

Indice

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