BIO RAD ChemiDoc Touch Manuale utente

ChemiDoc™Touch Imaging System

with Image Lab™Touch Software

User Guide

Version 1.2

ChemiDoc™ Touch Imaging System

with Image Lab™ Touch Software

User Guide

Version 1.2

Bio-Rad Technical Support

The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00

PM, Pacific time. Go to www.consult.bio-rad.com for worldwide technical support.

Phone: 1-800-424-6723, option 2

Web: www.consult.bio-rad.com

Email: Support@Bio-Rad.com (U.S./Canada only)

For technical assistance outside the U.S. and Canada, contact your local technical support office.

Notice

No part of this publication may be reproduced or transmitted in any form or by any means, electronic or

mechanical, including photocopy, recording, or any information storage or retrieval system, without

permission in writing from Bio-Rad.

Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to

change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or

omissions, or for any damage resulting from the application or use of this information.

Credits

Alexa Fluor, Coomassie Fluor, Qdot, SYBR, and SYPRO are trademarks of Life Technologies

Corporation. Coomassie is a trademark of BASF Aktiengesellschaft. Cy2 is a trademark of GE Healthcare.

DyLight and Krypton are trademarks of Thermo Fisher Scientific Inc. Excel, PowerPoint, and Windows are

trademarks of Microsoft Corporation. Mac and Numbers are trademarks of Apple Inc. GelGreen and

GelRed are trademarks of Biotium, Inc. Mitsubishi is a trademark of Mitsubishi Companies.

Precision Plus Protein standards are sold under license from Life Technologies Corporation, Carlsbad, CA,

for use only by the buyer of the product. The buyer is not authorized to sell or resell this product or its

components.

Criterion Stain Free gels are covered by U.S. Pat. No. 7,569,130.

Bio-Rad Laboratories, Inc. is licensed by Invitrogen Corporation to sell SYPRO products for research use

only under U.S. Patent Number 5,616,502.

Table of Contents

Safety and Regulatory Compliance 7

Important Safety Information 7

Warranty 8

General Precautions 9

Regulatory Notices 9

Alert Icons 10

Instrument Safety Warnings 11

Notice 12

Power Safety Information 13

Voltage Setting Information 13

Fuses 13

Chapter 1 Introduction 15

Product Features 15

Front Panel Components 17

Rear Panel Components 18

CCD Camera and Lenses 18

Introduction to Image Lab Touch Software 18

Emission Filters 19

Optional Accessories 19

Printer 19

White Sample Tray 19

User Guide | iii

Table of Contents

Blue SampleTray 19

Supported Tray Types 20

Technical Specifications 21

Environmental Requirements 23

Chapter 2 Image Lab Touch Software Overview 25

Starting Image Lab Touch 25

Setting the System Date and Time 26

Touch Screen Actions 29

The Application Interface 29

About the Live View Screen 31

The Image Lab Touch Main Toolbar 32

Tabs 33

About the Image View Screen 34

The Image View Toolbar 36

The Gallery 36

Gallery Screen Components 38

Getting Help 38

Entering Text on Screen 39

Using the On-Screen Keypad 39

Using the On-Screen Keyboard 40

Editing the Welcome Screen 40

Setting the Sound Volume 42

Chapter 3 Acquiring an Image 43

About Sample Trays 43

Workflow for Acquiring a Chemiluminescent Image 46

Specifying the Image Size 46

Choosing the Application 48

iv | ChemiDoc Touch Imaging System

Table of Contents

About Exposure Times for Chemiluminescent Images 48

The Image Resolution/Sensitivity Scale 49

Specifying the Region of Interest for Auto Exposure 54

Setting the Exposure Time for a Chemiluminescent Image 56

Setting the Exposure Automatically 56

Setting the Exposure Manually 58

Signal Accumulation Mode 59

Configuring Signal Accumulation Mode 60

Acquiring Images 60

Workflow for Acquiring Gel and Blot Images 62

Specifying the Image Size 63

Choosing the Application 63

Setting the Exposure Time 64

Acquiring an Image 65

Deleting Images 66

Chapter 4 Viewing Images 67

About Image View 69

About the Gallery 70

Viewing Images in Image View 71

Selecting and Opening Images in the Gallery 72

Adjusting How Images Are Displayed 75

Light Intensity Histogram 75

Restoring Default Settings 78

Checking Intensity Values 79

About Merging Images 80

Comparing Images 81

Searching for Images in the Gallery 84

User Guide | v

Table of Contents

Printing Images 84

Merging Images 85

Naming Merged Images 86

Renaming Images 87

Deleting Images 88

Viewing Image Information 89

Adding Notes to an Image 90

Chapter 5 Excising Bands 91

Excising Bands from Samples 91

Excising Bands on a Chemi/UV/Stain-Free Tray 91

Excising Bands on a White or Blue Tray 97

Chapter 6 Exporting Images 99

Exporting to a USB Flash Drive or External Hard Drive 100

Exporting to a Shared Folder 101

Disconnecting from a Shared Folder 103

Appendix A Maintaining the Imaging System 105

Updating Image Lab Touch Software 105

Reinstalling Image Lab Touch 105

About the UV Transilluminator Bulbs 106

When Transilluminator Bulbs Fail 106

Replacing the UV Transilluminator Bulbs 107

Replacing the Fuses 109

Appendix B Ordering Information 113

Appendix C Exporting to a Shared Folder 117

vi | ChemiDoc Touch Imaging System

Safety and Regulatory Compliance

Important Safety Information

Please read these instructions before operating the ChemiDoc™ imager.

This instrument is suitable for research use only. Therefore, it must be used only by

specialized personnel who know the health risks associated with the reagents that are

normally used with this instrument.

WARNING: The imaging of some applications involves UV illumination.

This instrument should be used only by trained personnel who know the

health risks associated with the UV radiation normally associated with this

instrument. Users should be trained on the appropriate personal protection

gear for working with UV light to minimize UV exposure.

To perform band excision using the Chemi/UV/Stain-Free tray, the transilluminator drawer

is pulled out with the UV source enabled. This exposes the user to UV radiation, which can

cause permanent damage to the eyes and skin. In its lowered position, the instrument’s

acrylic shield provides UV protection. However, in its raised position, it does not provide

complete protection to the user, and it does not protect others who are standing in the area

around the imager.

User Guide | 7

Safety and Regulatory Compliance

To protect users and bystanders, these procedures must be followed:

nProtect all skin surfaces (including the neck, ears, and hands). Before performing band

excision, the user and anyone near the imager must put on protective gear including

UV protective safety glasses, a face shield, lab coat, and gloves. A typical and

reasonable expectation of use is three operations per user a day for three minutes

each.

nBystanders without protective gear must stand at least 1.5 meters (five feet) away from

the imager and limit their exposure to no more than one hour per day.

Note: There is no exposure to UV radiation with the blue or white trays. No protective

gear is necessary when excising bands with these trays.

Warranty

The ChemiDoc Touch imaging system is warranted against defects in materials and

workmanship for one year. If any defect occurs in the instrument during this warranty

period, Bio-Rad Laboratories, Inc. will repair or replace the defective parts at its discretion

without charge. The following defects, however, are specifically excluded:

nDefects caused by improper operation

nRepair or modification done by anyone other than Bio-Rad Laboratories, Inc. or the

company’s authorized agent

nUse of spare parts supplied by anyone other than Bio-Rad Laboratories, Inc.

nDamage caused by accident or misuse

nDamage caused by disaster

nCorrosion caused by improper solvents or samples

8 | ChemiDoc Touch Imaging System

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