Biotix XPipette Manuale utente

xPIPETTE™

Table of Contents Introduction

Introduction

Parts Checklist ........................................ 3

GLP Compliance ..................................... 3

Description .............................................. 3

Specications .......................................... 4

Setting Volume

Instructions .............................................. 6

Pipetting

Tip Selection ............................................ 8

Tip Mounting ............................................ 8

Operation ................................................. 8

Dispensing ............................................... 8

Good Pipetting Guidelines ....................... 9

Pre-rinsing ............................................... 9

Pipetting Liquids of Varying Densities ...... 9

Temperature Considerations ...................... 9

Reverse Pipetting .................................. 10

Troubleshooting

Troubleshooting and Repairs ................. 11

Sample Splash ...................................... 12

Leaks, Inaccuracy, Abnormal Stroke ..... 12

Service, Calibration and Repair ............. 12

Maintenance

Autoclaving ............................................ 13

Tip Ejector Arm Removal ....................... 13

Storage .................................................. 13

Acids and Corrosives ............................. 13

Parts ....................................................... 13

Accessories

Pipette Carousel .................................... 14

Replacement Parts ................................ 14

Warranty

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Table of Contents Introduction

xPIPETTE is an air-displacement pipette that incorporates

major ergonomic improvements to minimize strain and

help reduce the risk of repetitive stress injuries. Low spring

forces and low force seals contribute to a lighter feel. A

magnet is used to help balance and hold the piston in the

zero position, which reduces static force on the operator’s

hand during the pipetting cycle.

Parts Checklist

After unpackaging, please verify that the following items

were included and are undamaged:

• xPIPETTE

• xPIPETTE User Guide

• Calibration Certicate

GLP Compliance

The unique pipette serial number is

engraved on the body of the pipette

underneath the handle. It provides

a unique identication to to help

fulll GLP requirements.

Calibration certicates are also

included for further traceability.

Description

Connecting Nut

Shaft

Stainless Steel

Tip Ejector Arm

Digital Volume Indicator

Plunger

Body:

high-impact, low thermal

conductivity polymer

Handle

Volume Lock

Tip Ejector

2 μL

1.25 μL

10 μL

7.5 μL

20 μL

12.5 μL

100 μL

75 μL

200 μL

125 μL

300 μL

225 μL

1000 μL

0.75 mL

67 μL 108 μL 157.6 μL

xPIPETTE Manual

xPIPETTE™

Specications

Single Channel Pipettes

BIOTIX ISO 8655

Volume* Systematic

Error

Random

Error

Systematic

Error

Random

Error

2 μL with xTIP4 20 μL tips (P/N 63300160)

0.2 ± 0.024 ≤ 0.012 ± 0.08 ≤ 0.04

1± 0.027 ≤ 0.013 ± 0.08 ≤ 0.04

2± 0.03 ≤ 0.014 ± 0.08 ≤ 0.04

10 μL with xTIP4 20 μL tips (P/N 63300161)

1± 0.025 ≤ 0.012 ± 0.12 ≤ 0.08

5 ± 0.075 ≤ 0.03 ± 0.12 ≤ 0.08

10 ± 0.1 ≤ 0.04 ± 0.12 ≤ 0.05

20 μL with xTIP4 20 μL tips (P/N 63300162)

2± 0.15 ≤ 0.04 ± 0.20 ≤ 0.10

10 ± 0.15 ≤ 0.05 ± 0.20 ≤ 0.10

20 ± 0.2 ≤ 0.06 ± 0.20 ≤ 0.10

100 μL with xTIP4 200 μL tips (P/N 63300163)

10 ± 0.35 ≤ 0.10 ± 0.80 ≤ 0.30

50 ± 0.4 ≤ 0.12 ± 0.80 ≤ 0.30

100 ± 0.8 ≤ 0.15 ± 0.80 ≤ 0.30

200 μL with xTIP4 200 μL tips (P/N 63300164)

20 ± 0.5 ≤ 0.20 ± 1.60 ≤ 0.60

100 ± 0.8 ≤ 0.25 ± 1.60 ≤ 0.60

200 ± 1.6 ≤ 0.30 ± 1.60 ≤ 0.60

300μL with xTIP4 300 μL tips (P/N 63300327)

30 ± 0.75 ≤ 0.30 ± 2.40 ≤ 0.90

150 ± 1.2 ≤ 0.375 ± 2.40 ≤ 0.90

300 ± 2.4 ≤ 0.45 ± 2.40 ≤ 0.90

1000μL with xTIP4 1000 μL tips (P/N 63300165)

100 ± 3 ≤ 0.6 ± 8.0 ≤ 3.0

500 ± 4 ≤ 1 ± 8.0 ≤ 3.0

1000 ± 8 ≤ 1.5 ± 8.0 ≤ 3.0

These manufacturer’s specications should be used as

guidelines when establishing your own performance

specication.

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Specications

Multi-Channel Pipettes

BIOTIX ISO 8655

Volume* Systematic

Error

Random

Error

Systematic

Error

Random

Error

10 μL with xTIP4 20 μL tips

(P/N 63305170 & 63305174)

1± 0.040 ≤ 0.05 ± 0.08 ≤ 0.10

5 ± 0.075 ≤ 0.075 ± 0.15 ≤ 0.15

10 ± 0.100 ≤ 0.05 ± 0.20 ≤ 0.10

20 μL with xTIP4 20 μL tips

(P/N 63305171 & 63305175)

1 ± 0.15 ≤ 0.08 ± 0.30 ≤ 0.16

5± 0.15 ≤ 0.15 ± 0.30 ≤ 0.30

10 ± 0.20 ≤ 0.10 ± 0.40 ≤ 0.20

200 μL with xTIP4 200 μL tips

(P/N 63305172 & 63305176)

2 ± 0.50 ≤ 0.24 ± 1.00 ≤ 0.48

10 ± 0.80 ≤ 0.25 ± 1.60 ≤ 0.50

20 ± 1.60 ≤ 0.60 ± 3.20 ≤ 1.20

300 μL with xTIP4 300 μL tips

(P/N 63305173 & 63305177)

10 ± 0.75 ≤ 0.10 ± 1.50 ≤ 0.20

50 ± 1.20 ≤ 0.12 ± 2.40 ≤ 0.30

100 ± 2.40 ≤ 0.15 ± 4.80 ≤ 0.30

*Specications are subject to change without notice

* All volumes are in microliters

xPIPETTE Manual

xPIPETTE™

Setting the Volume

Unlocked Locked

Volume is locked when

wings are straight across

Wings

Instructions

1. Turn the volume lock counter-clockwise to the position

shown at left below so the volume setting mechanism is

unlocked and free to turn.

2. With the mechanism unlocked, orient the pipette so you

are looking at the digital volume indicator, then rotate the

plunger button to change volume – counter-clockwise to

increase, clockwise to decrease volume.

The volume indicator is read from the top down and is

color-coded in relation to where the decimal point should

go.

2-20 μL: Black - μL.

Red- tenths, hundredths of μL.

100 -300 μL: All digits black - whole μL.

1000 μL: Red - mL. Black - tenths, hundreds of mL.

3. To eliminate errors due to mechanical backlash: when

setting the desired volume, rst turn the knob 1⁄3 turn

above the desired volume. Then turn the knob slowly

clockwise until the desired volume is displayed.

4. Turn the volume lock clockwise (see diagram above) to

prevent any accidental changes to the volume setting.

Example volumes are shown on the next page (note the

intermediate setting at the right).

2 μL

1.25 μL

10 μL

7.5 μL

20 μL

12.5 μL

100 μL

75 μL

200 μL

125 μL

300 μL

225 μL

1000 μL

0.75 mL

67 μL 108 μL 157.6 μL

2 μL

1.25 μL

10 μL

7.5 μL

20 μL

12.5 μL

100 μL

75 μL

200 μL

125 μL

300 μL

225 μL

1000 μL

0.75 mL

67 μL 108 μL 157.6 μL

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Setting the Volume

Volume ranges and increments for single-channel pipettes

are shown below:

Volume ranges and increments for multi-channel pipettes are

shown below:

Pipette Size

(μL)

Adjustable

Range

(μL)

Recommended

Volume Range

(μL)

Increment

(μL)

2 0 - 2 0.1 - 2 0.002

10 0 - 10 0.5 - 10 0.02

20 0 - 20 2 - 20 0.02

100 0 - 100 10 - 100 0.2

200 0- 200 20 - 200 0.2

300 0 - 300 30 - 300 0.5

1000 0 - 1000 100 - 1000 2.0

Pipette Size

(μL)

Adjustable

Range

(μL)

Recommended

Volume Range

(μL)

Increment

(μL)

10 0 - 10 0.5 - 10 0.02

20 0 - 20 2 - 20 0.02

200 0- 200 20 - 200 0.02

300 0 - 300 30 - 300 0.5

xPIPETTE Manual

xPIPETTE™

Pipetting

Tip Selection

To ensure top performance of your xPIPETTE, we recom-

mend that you use Biotix xTIP4 pipette tips. Together, the

pipette-tip combination creates an ergonomic system to

reduce insertion and ejection forces. The xPIPETTE is also

fully compatible with Rainin®LTS™tips.

Tip Mounting

Single-Channel Pipette: To mount a tip, press the pipette

shaft nozzle into the end of the tip with light force.

Multi-Channel Pipette: Align the shaft nozzles into the row

of tips, holding the pipette at an angle. Position the pipette

upright and press the nozzles into the tips until securely

mounted.

Operation

Before pipetting valuable samples, we recommend that you

practice aspirating and dispensing water before pipetting

with actual samples.

1. Set the desired volume as described on page 7.

2. Attach a new tip. Press the shaft into the tip to make a

good seal.

3. Press the plunger button to the FIRST STOP, and hold it in

this position. The magnetic assist will help you sense and

hold this position.

4. Holding the pipette vertically, place the tip into the sam-

ple to the proper depth and relax your thumb pressure

on the plunger. The light piston spring will move the

piston upward, aspirating your sample. Do not let go of

the plunger button suddenly, or the piston may snap up

quickly, resulting in an inaccurate measurement.

5. Pause briey to ensure that the full volume of the sample

is drawn into the tip.

6. Withdraw the tip from the sample. If any liquid remains

on the outside of the tip, wipe it carefully with a lint-free

tissue, taking care not to touch the tip orice.

Dispensing:

1. Touch the tip end against the sidewall of the receiving

vessel and press the plunger slowly, past the rst stop, to

blowout (bottom of stroke).

First Stop

Blowout

Rest Position

Step 1 Step 2

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Pipetting

Wait: 1-2 seconds for 2–300 μL volumes, 2-3 seconds for

1000 μL (longer for viscous solutions).

2. Still holding the plunger, withdraw the tip, sliding it along

the wall of the vessel. Release the plunger.

3. Press the tip ejector button to discard the tip. Use a new

tip for each sample to prevent carry-over. Repeat for the

next pipetting cycle.

Good Pipetting Guidelines

xPIPETTE incorporates features to deliver pipetting

consistency. You should also maintain:

1. Consistent pickup and dispense rhythm.

2. Consistent speed and smoothness when pipetting.

3. Consistent pressure on the plunger button at the rst

stop.

4. Consistent immersion depth.

5. Pipette vertically, or within 20° of vertical.

6. Don’t invert or lay the pipette at with liquid in the tip.

Pre-Rinsing Recommended

Some solutions may leave a lm on the inside tip wall. This

lm remains relatively constant in successive pipetting with

the same tip, so excellent precision can be obtained by

relling the tip and using the relled volume as the sample.

Successive samples from this same tip will exhibit good

reproducibility.

Temperature Considerations

Warm or cold liquids can be measured with good preci-

sion by using a consistent pipetting rhythm. This will help

minimize any dierences in heating or cooling eects within

the pipette. Use a new pipette tip each time for optimal

accuracy and precision when measuring samples with

temperatures greatly dierent from ambient, and do not

pre-rinse. As with any air-displacement pipette, you will get

the best results if there is no delay between aspirating the

sample and dispensing it.

Volume Range (μL) Immersion Depth

(mm)

Wait Time

(sec)

0.1-10 1-2 1

10-200 2-3 1

200-1000 3-6 2-3

xPIPETTE Manual

1 0

xPIPETTE™

Pipetting Liquids of Varying Density

xPIPETTE adjustable pipettes allow you to compensate for

solutions with densities that are dierent than water, by

setting the volume slightly higher or lower. The compensa-

tion amount must be determined empirically. For example,

if pipetting 10 μL of CsCl solution, you determine that the

volume delivered is actually 8.5 μL. Change the volume set-

ting to 11.8 μL and repeat the measurements. If the volumes

delivered are still not close enough to 10 μL, make another

slight volume adjustment until the measurements are as

desired.

Reverse Pipetting

Another technique for reducing error due to lm retention,

especially

useful for more viscous liquids, is reverse pipetting. The

operating sequence is reversed:

1. Mount a disposable tip on the pipette shaft nozzle.

2. Press the plunger button fully to the SECOND STOP.

3. Immerse the tip in liquid and allow the button to return

slowly to the resting position. Wait a moment for the liquid

column in the tip to reach equilibrium.

4. Wipe any excess liquid from the outside of the tip without

touching the orice.

5. To dispense, rest the end of the tip against the vessel wall

and press the plunger to the rst stop. Hold this position

for a few seconds, or long enough for the liquid column to

reach equilibrium again.

6. Remove the tip from the receiving vessel without blowing

out the remaining liquid.

7. Return excess sample in the tip to the original sample

container, if desired. Discard the used tip.

Troubleshooting

First Stop

Blowout

Rest Position

Step 2 Step 3 Step 5 Step 7

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