BPC instruments AMPTS III Manuale utente
s
www.bpcinstruments.com
Mobilvägen 10, 223 62 Lund, Sweden
Contact Us :
+46 (0)46 163950
OPERATION AND
MAINTENANCE MANUAL
AMPTS III
Anaerobic batch fermentation test systems
2
Version 1.0 April 2023
The latest version of this manual can always be provided upon request:
support@bpcinstruments.com
Any questions related to this document should be directed to:
BPC Instruments AB
Mobilvägen 10
SE-223 62 Lund
Sweden
Telephone: +46 (0)46 163950
Email: info@bpcinstruments.com
Web: https://www.bpcinstruments.com
This document contains proprietary information protected by copyright. No part of this
publication may be redistributed in any form whatsoever or translated into any language
without prior, written permission of BPC Instruments AB.
© 2023 BPC Instruments AB. All rights reserved.
Produced in Sweden.
3
LIST OF CONTENTS
General Information…………………………………………………………………………………………………..
4
Chapter 01: Overview…..…………………………………………………………………………………………….
6
Chapter 02: Delivery Checks……………………………………………………………………………………….
8
Chapter 03: Equipment Description……………………………………………………………………………
10
Chapter 04: Setting up the Instrument……………………………………………………………………….
14
Chapter 05: AMPTS III and AMPTS III Light DUO………………………………………………………….
20
Chapter 06: AMPTS III Web-based Software……………………………………………………………….
22
6.1 Computer Network Configuration………………………………………………
22
6.2 Network Quick Guide………………………………………………………………….
22
6.3 Web-based Software AuroraTM…………………………………………………..
23
Chapter 07: Maintenance and Spare Parts………………………………………………………………….
27
4
GENERAL INFORMATION
Before operating the AMPTS III (hereafter referred to as “the instrument”, “the system” or “the equipment”
interchangeably) from BPC Instruments AB (hereafter referred to as “BPC Instruments”, “BPC”, “Bioprocess
Control” or “BPC Instruments AB” interchangeably), carefully read this operator manual for the instrument, any
separate instructions for other equipment used together or in conjunction with the instrument, as well as the
safety instructions for any and all chemicals used in the process of utilising the instrument.
Safety Information
When performing experiments with the instrument, always use protective eyewear, gloves, and lab coat. Always
make sure there is adequate ventilation and take proper precautions when handling electrical devices near
water or explosive gases. Make sure to tie back any hanging objects, such as hair and clothing, when working
near rotating or other moving parts.
Do not modify the instrument without the prior consent of the manufacturer. BPC Instruments AB do not assume
responsibility for any errors due to equipment modification.
Do not clean or service the instrument while it is running.
Do not expose the instrument to mechanical vibrations or high frequency radio transmissions.
Never operate the instrument in a way it was not intended.
Never operate the instrument or let anyone else operate it without proper training.
Never use the instrument outside or in environments with parameters outside of the instruments recommended
range.
Never connect additional electrical equipment not supplied by BPC Instruments AB for the express purpose of
using with the instrument. This is true even if the connections can mate.
Always back-up important data to an external device.
Always keep the instrument level and on a flat and stable surface. Failing to do so can, among other things,
generate an erroneous gas reading.
Always make sure all safety guards are in place and working before operating the instrument.
Always make sure that all parts are functioning properly immediately after start-up.
Always keep the instrument clean.
Always make sure to have access to relevant chemicals before starting an experiment.
Always dispose of parts and chemicals according to applicable rules in the country of usage.
Periodic maintenance of the instrument and its various accessories is essential. Always make sure they are in
working condition. If service or spare parts are required, please visit https://webshop.bpcinstruments.com or
contact BPC Instruments AB directly or one of its representatives.
Always make sure to connect the power supply so that it is easy to remove from the mains power outlet and so
it doesn’t risk becoming damaged.
5
Always make sure that the gas outlet of the Flow Cell Unit (FCU) is able to release pressure in the event of
pressure build-up inside of the instrument. Do not obstruct or block it.
Always wait 60 seconds between powering the system on and off. This will allow for the operating system to
shut down properly and for the capacitators to properly cycle.
Always use deionized water to minimise the risk of residue or rust forming on the inside of the FCU.
Limited Warranty
The product warranty provided with the instrument corresponds to the stipulations in Orgalime S 2012, unless
otherwise agreed upon with BPC Instruments AB. BPC Instruments AB reserves the right to correct any possible
errors, mistakes, changes, updates, technical data or otherwise relevant information in this manual or any other
documents, where applicable by law.
Electrical Safety
Compliance is required with respect to voltage, frequency and current requirements indicated on relevant parts.
Improper operation, damage to the equipment, fire or otherwise undesired effects might be caused by
connecting to a different power source. There are no user-serviceable parts in the equipment, unless otherwise
agreed upon with BPC Instruments AB.
Before Getting Started
Read this manual before installing and using the instrument. In addition, keep this instruction manual for future
reference and make sure it is easily available for people who regularly use the system.
Contact Information
BPC Instruments
Mobilvägen 10
223 62 Lund
Sweden
E-mail: sales@bpcinstruments.com
Phone: +46 (0)46 163950
Website: https://www.bpcinstruments.com
Webshop: https://webshop.bpcinstruments.com
Swedish organisation number: 556687-2460
6
Chapter 01: Overview
The Automatic Methane Potential Test System III (AMPTS III) is the next generation of the
well-established analytical platform for conducting anaerobic batch fermentation tests
developed by BPC Instruments. This new system comes with the best features of the previous
generation and a series of improvements and further developments in all the different parts
of the equipment.
AMPTS III was designed to cover a broad range of applications, including:
-Biochemical Methane Potential (BMP) tests
-Biogas production (Gas composition based on methane and carbon dioxide)
-Specific Methanogenic Activity (SMA) assays
-Residual Gas Potential (RGP) assays
-Biological Hydrogen Potential (BHP) tests
In addition, this instrument is suitable to optimise relevant parameters involved in the
Anaerobic Digestion (AD) process, providing key information regarding the optimum mix of
substrates for co-digesting processes, proper pre-treatment methods, the need for additives,
etc. During the degradation of the organic material under anaerobic condition, the produced
gas is continuously collected and measured when working with AMPTS III, which makes it
possible to determine the dynamic degradation profile of any biomass substrate.
Regarding the application, the main difference between AMPTS III and AMPTS II (previous
version) is the possibility to perform single (e.g., methane) and total gas (e.g., biogas)
measurements. In this case, users have the possibility to measure both biogas and methane
in the same batch test for estimation of gas composition. The gas measured can be easily
collected for further composition analysis.
AMPTS III retains the features of the previous version (AMPTS II), which include the following:
-Same configuration in two versions (AMPTS III and AMPTS III Light).
-Automated sampling, recording, data analysis and report generation.
7
-Standalone instrument with embedded data acquisition and web server for remote
access (no need for software installation).
-The measuring cell is pre-calibrated by the factory.
-The software can be accessed locally or remotely from any device with a web browser
(preferably Google Chrome).
-Option for real-time automatic compensation for atmospheric pressure,
environmental temperature and moisture content changes to normalise the data to
standard conditions.
-Function to remove gas overestimation and automatic calculation of the amount of
substrate and inoculum needed per reactor in order to perform BMP tests.
-Reliable operation and easy maintenance where most of the components can be easily
exchanged without special requirements.
-System log for operational diagnosis.
Table 1 summarizes the new features of AMPTS III and AMPTS III Light:
Table 1. Overview of the new features of AMPTS III and AMPTS III Light.
AMPTS III and AMPTS III Light
INCUBATION UNIT
-18 channels (AMPTS III) and 9 channels (AMPTS III Light)
-AMPTS III standard configuration with 1 L reactors (0.5 L
reactors as an option).
-AMPTS III Light comes with 2 L reactors
-New thermostatic water bath with capacity to place 18 (0.5 /
1 L) or 9 (2 L) reactors
CO2-ABSORPTION UNIT
-Improved bottle holder design for CO2 removal bottles
-AMPTS III comes with two bottle holder units, each for nine
bottles, allowing more flexibility to arrange the instrument
-Higher capacity for CO2 removal with 250 mL sized bottles
(AMPTS II offered 100 mL bottles).
BPC CORE AMPTS UNIT AND
SOFTWARE
-New design where each flow cell is enclosed in a chamber
-The flow cell chambers can be easily replaced without tools
-Two possible measurement resolutions (2 and 9 mL)
-New electronics for faster and more stable operation
compared to the previous version
-150 times higher storage capacity compared to AMPTS II
-USB port for software upgrade
-Addition of a power button
-Factory reset function
-OLED screen to easy access the status and IP address of the
instrument.
-Built-in accelerometer for leveling of the unit C
-New software platform AuroraTM
-It is possible to start and stop all channels with one click
-Three types of normalisation
-New set of settings to visualise data and generate reports
8
Chapter 02: Delivery Checks
AMPTS III (Figure 1) and AMPTS III Light (Figure 2) are delivered with the components listed
below:
Figure 1. AMPTS III Complete System.
Unit A – Incubation Unit
20 glass reactors (0.5 or 1 L)
1 motor controller signal cable
1 MCU power adapter
18 brushless DC motors
1 motor power splitter
18 axis couplings for brushless
DC motors
1 thermostatic water bath
1 base tray
19 brushless DC motor cables
250 mm (15 units)
500 mm (3 units)
1500 mm (1 unit)
20 stirrers GL 45 (0.5 or 1 L)
standard
1 thermostatic water bath lid
for 18 reactors (0.5 or 1 L)
1 motor controller unit
(MCU)
20 push-in valves 6 mm
Unit B – CO2-absorption Unit
2 bottle holders 9 x 250 mL
20 bottle nuts GL 45
20 glass bottles (250 mL)
20 lids GL 45
Unit C – BPC Core AMPTS Unit
1 BPC Core AMPTS
40 check valves
1 main unit power adapter
20 flow cell units (FCUs)
1 plastic syringe
1 ethernet cable
Additional Components
1 Festo tubing 50 m
40 push-in connectors 6 mm
1 bottle/tube opening tool
1 funnel
12 soft binders 7/180 mm
FCU volume sheet (2 or 9
mL)
20 tubing stoppers
40 multi-coloured marker clamps
Accessories
-More robust and less gas permeable tubing
-Tubing connectors to simplify and ensure a good and tight
connection between the tubing and the inlet of the flow cell
chamber and the outlet of the reactor
-Push-in valves to simplify the flushing step of the headspace
of the reactor in order to create an anaerobic environment
-New tubing markers for easy identification of the different
channels
-New tool for removing lids and tubing
9
Figure 2. AMPTS III Light Complete System.
Unit A – Incubation Unit
10 glass reactors (2 L)
1 motor controller signal cable
1 MCU power adapter
9 brushless DC motors
1 motor power splitter
9 axis couplings for brushless
DC motors
1 thermostatic water bath
10 brushless DC motor cables
250 mm (6 units)
500 mm (3 units)
1500 mm (1 unit)
10 stirrers GL 45 (2 L) standard
1 thermostatic water bath lid
for 9 reactors (2 L)
1 motor controller unit
(MCU)
10 push-in valves 6 mm
Unit B – CO2-absorption Unit
1 bottle holders 9 x 250 mL
10 bottle nuts GL 45
10 glass bottles (250 mL)
10 lids GL 45
Unit C – BPC Core AMPTS Light Unit
1 BPC Core AMPTS
20 check valves
1 main unit power adapter
10 flow cell units (FCUs)
1 plastic syringe
1 ethernet cable
Additional components
1 Festo tubing 50 m
20 push-in connectors 6 mm
1 bottle/tube opening tool
1 funnel
6 soft binders 7/180 mm
FCU volume sheet (2 or 9
mL)
20 tubing stoppers
20 multi-coloured marker clamps
The following items are NOT provided in the package of both instruments:
-Flushing gas to obtain anaerobic conditions inside the reactors during the sample
preparation phase.
-3 mol/L sodium hydroxide (NaOH) solution.
-pH indicator (Thymolphthalein) and ethanol.
-Additional wall socket adapters (plugs/contacts). The ones supplied are according to
European, US or UK standards, depending on the country where the instrument will
be operated.
-Gas sampling units and gas bags for off-line gas composition analysis.
10
Chapter 03: Equipment Description
AMPTS III and AMPTS III Light consist mainly of three parts: Incubation unit, CO2-absorption
unit and detection unit (also called gas volume measuring device).
1) Incubation Unit (Unit A): Here is where the fermentation process takes place (Figure
3). 18 (0.5 or 1 L) or 9 (2 L) reactors are placed inside a thermostatic water bath (68 x
56 x 33 cm), which is used for temperature control (up to 60° C, precision of 0.2° C).
The thermostatic water bath is provided with a lid for 18 or 9 reactors to minimize
water loss and ensure that the temperature set point is rapidly reached. The
incubation system provides a well-controlled environment in regard to temperature,
pH, and relative humidity for biogas/biohydrogen production.
Figure 3. (A) Incubation unit for AMPTS III and (B) AMPTS III Light.
Each reactor is connected to a brushless DC motor that ensures a well-mixed content
through a strong, reliable, and multifunctional agitation system (Figure 4). A motor
controller provides the power via motor cables, where all the motors receive the same
information. The MCU needs to be turned off and unplugged from the power source
when any cables are connected or disconnected from the motors.
Figure 4. Main features of the agitation system in AMPTS III and AMPTS III Light.
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