CEM Razor Manuale utente

Razor®

Rapid Peptide Cleavage System

Manual

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Contents

Safety

Safety Notations 1

Introduction

About the Instrument 2

Recommended Cleavage Parameters 2

Important Safeguards

Instrument Safeguards 3

Instrument Overview

Razor Hardware 4

Accessory Kit 5

System Installation

System Setup 6

Assign the Set Temperature 8

Peptide Cleavage

Prepare the Razor 9

Rinse Resin Prior to Cleavage 10

Cleavage 12

Precipitation and Analysis 12

Sample Position Cleaning 13

Maintenance

Cavity Ceiling Cleaning 14

Sample Position Clog 14

System Specifications

Location 15

Electrical Requirements 15

Environmental Constraints 15

Warranty

Limited Warranty Information 16

Technical Assistance

Applications Support 18

Technical Support 18

Contact Information

CEM Corporation Headquarters 19

CEM International Subsidiaries 19

CEM Distributors 20

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1Safety

Safety

Safety Notations

This manual uses three safety alert words at points in the documentation where the user should be aware of

potential hazards. The safety alerts are shown in color-coded boxes. The three words – NOTE, CAUTION, and

WARNING – indicate differing levels of observation or action as described below:

NOTE

A NOTE is intended to provide emphasis on procedures that may be misinterpreted or overlooked, or to

clarify confusing situations.

CAUTION

A CAUTION is intended to provide essential information and to emphasize procedures that, if not strictly

followed, may result in improper instrument operation.

WARNING

A WARNING is intended to emphasize dangerous or hazardous conditions that may result in personal

injury to the user and/or damage or destruction of the instrument.

2 Introduction

Introduction

About the Instrument

The Razor parallel cleavage system is an isolated heating module designed for performing the peptide cleavage

step. The isolated module provides the advantage of protecting the Liberty Blue™system from the harsh reagents

required for the cleavage all while allowing the use of heat to provide high quality peptides in excellent yields in a

fraction of the time.

This manual is intended for use by both novice and experienced users for the operation and maintenance of the

Razor.

Recommended Cleavage Parameters

In Fmoc synthesis, the removal of the peptide from the solid support is typically accomplished with triuoroacetic

acid (TFA). Because the side chain protecting groups used in Fmoc synthesis are acid labile, a single step both

cleaves the peptide from the resin and removes the protecting groups. Various scavenger molecules are added

to the TFA to prevent the cleaved protecting groups from reattaching to the peptide. The particular scavengers

used depend on the specic peptide sequence. Common scavengers include water (scavenges t-butyl cations),

triisopropyl silane (TIS, scavenges trityl and Pbf cations), ethane dithiol (EDT, scavenges t-butyl cations, reduces

oxidation of Cys/Met side chains), dioxa-1,8-octane-dithiol (DODT, scavenges t-butyl cations, suppresses oxidation

of Cys/Met side chains), phenol (protects Tyr and Trp side chains from oxidation), and thioanisole (aids in removal

of Pbf protecting groups from Arg(Pbf), suppresses oxidation of Cys/Met side chains).

CEM recommends using a cocktail of TFA/TIS/H2O/DODT (92.5/2.5/2.5/2.5).

3Important Safeguards

Important Safeguards

General guidelines for safe operation of the Razor system are presented below and all specic safety messages

are located throughout the manual.

Instrument Safeguards

• Service can only be performed by an authorized CEM service technician. The system must be disconnected from

power supply prior to servicing.

• Do not tamper with the Razor, including actions such as removing any components of the housing.

• The Razor must be installed in a ventilated fume hood pulling at least 30.5 CFM.

• Adequate ventilation should be provided for preparation of reagents and solvents for use on the system.

• If any damage to the instrument is noted, do not attempt instrument operation.

• Cleavage vessels must be purchased directly from CEM Corporation or through its authorized dealer network.

• If it is necessary to use an extension cord, use only a 3-wire extension cord that has a 3-blade grounding plug

and a 3-slot receptacle that will accept the plug from the instrument. The marked rating of the extension cord

must be equal to or greater than the electrical rating of the instrument.

• If instrument is used in a manner not specied by CEM Corporation in this manual, then the protection provided

by the equipment may be impaired.

• Proper precautions must be taken to avoid contact with reagents or reagent vapors. Protective gear should be

worn as outlined in the user’s safety program for hazardous materials and the reagent manufacturer’s safety

data sheet. Refer to these guidelines for proper handling and disposal of the reagents. Dispose of all waste in

accordance with all applicable local, state, and federal health and safety recommendations.

• Do not position the Razor so it is difcult to access the power, heat or vacuum switches

4 Instrument Overview

Instrument Overview

For a list of parts and part numbers, visit our website at www.cem.com. A list of parts can be found on the

“Products” page under “Razor.”

Razor Hardware

Item Item

1 Vessel Hot Block 7 Light Switch

2 Valve Panel: Sample Positions, Vent, and Drain knobs 8 Power Switch

3Vessel Tray 9 Heat Switch

4 Temperature Control Panel 10 Vacuum Switch

5 Cavity 11 Vacuum Gauge

6 Locking Lever 12 Ports: Vent, Vacuum, and Waste

5Instrument Overview

Accessory Kit

Vessel Rack Vessel Rack Cover Waste Tubing Bundle

P/N 547205 P/N 170875 P/N 547225

GL45 Waste Cap Razor Power Supply Position Cleaning Tool

P/N 167545 P/N 267080 P/N 547300

1 L Glass Bottle (no cap) Vessel Caps 50 mL Centrifuge Tubes

P/N 167550 P/N 170810 P/N 330090 (Set of 25)

Cleavage Vessel Spill Tray

P/N 170897 (Pack of 60) P/N 170530

P/N 170900 (Pack of 120)

*Power Cord not pictured as it is region specic.

6 System Installation

System Installation

System Setup

1. Remove the Razor from it’s shipping box and place the system in a well ventilated fume hood.

WARNING

The Razor must be installed in a ventilated fume hood pulling at least 30.5 CFM.

2. Connect the power cord to the power supply box. Attach the power supply to the connector on the rear of the

system, and plug the power cord into an appropriate outlet.

3. Connect the waste tubing.

3.1. Connect the tubing bundle to the appropriate ports on the side of the system: vacuum and waste.

3.2. Attach the opposite end of the tubing bundle to the GL45 cap. Both ports on the cap are the same so

tube placement is indifferent.

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