Contichrom System Manual
page 3of 80
Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com
2.8. Methods for UV-sensors...................................................................................................31
Method UV AutoZero.mth................................................................................................................................31
Method Backflush_Detectors.mth ...................................................................................................................31
2.9. Other Methods.................................................................................................................32
Method Pause.mth ...........................................................................................................................................32
Frac_Home.mth ................................................................................................................................................32
2.10. Standard Procedures.....................................................................................................32
Load_Buffers.PRC .............................................................................................................................................32
Cleaning.PRC.....................................................................................................................................................32
2.11. Creating methods and performing runs .........................................................................33
2.12. Preparing and mounting the columns ............................................................................33
2.13. Mounting the pH flow cell and electrode .......................................................................34
2.14. Preparing The fraction collector.....................................................................................35
2.15. Operation in cold cabinet or cold room..........................................................................36
3. Operating the Contichrom system .............................................................................36
3.1. Recommended Column Sizes................................................................................................36
3.1. Preparing a procedure to be run...........................................................................................37
3.2. Procedure for Loading buffers ..............................................................................................38
3.3. Starting a run.......................................................................................................................38
3.4. Managing a run....................................................................................................................39
Refilling Buffers during a run ............................................................................................................................40
3.5. Monitoring a Run.................................................................................................................41
3.6. Programming Methods.....................................................................................................43
3.7. Evaluating a run ...............................................................................................................43
3.8. Log Book ..........................................................................................................................43
4. Cleaning the system..................................................................................................44
4.1. Simple Cleaning................................................................................................................44
4.2. Thorough Cleaning ...........................................................................................................44
5. Storing the system ....................................................................................................46
5.1. Storing the pH electrode...................................................................................................47
6. Maintenance ............................................................................................................47
6.1. Recommended Maintenance Intervals..............................................................................48
6.2. Calibrating the pH sensor..................................................................................................48
6.3. Pump Maintenance ..........................................................................................................50
Pump seal wash Solution Replacement............................................................................................................50
Pump Head Maintenance .................................................................................................................................50
Cleaning the Pump Head Assembly ..................................................................................................................51
