Cytiva 420100 Manuale utente

Pop-Top Membrane Holder 13 mm

Instructions for Use

Introduction

Important

Read these instructions carefully before using the products.

Intended use

The products are intended for research use only, and shall not be

used in any clinical or in vitro procedures for diagnostic purposes.

Safety

For use and handling of the products in a safe way, either refer to the

Safety section in these instructions or to the Safety Data Sheets

where applicable.

Description

Pop-Top Filter Holders are designed for filtration of small volumes of

liquids using positive pressure. These holders are used for filtering

particulate from a fluid or gas stream, or for concentrating the partic-

ulate onto a filter surface for further examination. Pop-Top Filter

Holders accommodate membrane filters such as polycarbonate,

PTFE, nylon, and cellulose ester-based media, as well as depth filters

such as glass fiber and fibrous polyester.

Pop-Top Filter Holders are easily connected together for serial filtra-

tion since the inlet is a female Luer slip fitting and the outlet is a male

Luer slip fitting. Prefilters (diameter 10 mm when used with a

membrane) may be used to extend membrane life.

The figure below shows an expanded view of the Pop-Top Filter

Holder.

Specifications

Material Clear polycarbonate

Maximum Operating Temperature

and Pressure

38°C (100°F) at 0.34 MPa (3.4

bar, 50 psi)

Autoclavable 121°C (250°F) for 15 minutes at

0.1 MPa (1.0 bar, 15 psi)

Holder Size 2.7 cm OD × 2.7 cm H

Membrane Diameter 13 mm

Prefilter Diameter 10 mm

Filtration Area 0.8 cm2

Connections

Cap Female Luer slip-fit

Base Male Luer slip-fit

Operating instructions

Assembly and Loading

Step Action

1 Remove the three parts of the Pop-Top holder from its plastic

bag.

2 If using a prefilter, place it in the center hole depression of the

Pop-Top holder cap before loading the membrane.

3 Remove the membrane with its thick, colored separator paper

from the storage box with blunt tipped forceps. Slip one tong of

the forceps between the membrane and paper.

NOTICE

It is important to use blunt tipped

forceps to avoid damaging the

membrane.

4 Pick up the Pop-Top base in one hand and place the membrane

on the support grid right side up.

Note:

Generally, the membrane is packaged with the top side of the

filter facing up, and you should maintain this orientation when

assembling it into the filter holder.

5 For liquid filtration, wetting the membrane with clean filtration

liquid can make it easier to load.

6 Position the Pop-Top cap over the base and press down until it

snaps in place.

cytiva.com 29690340 AB

Step Action

7 Slip the Pop-Top clamp ring over the cap. Slide the clamp ring

around the "fingers" of the cap until it is firmly seated.

Autoclaving

Pop-Top holders, both unloaded or loaded with Whatman™

membranes, can be repeatedly autoclaved at 121°C (250°F) for 15

minutes at 0.1 MPa (1.0 bar, 15 psi).

When autoclaving loaded Pop-Top holders, loosen the clamp ring

about half way from the cap fingers. Reseat clamp ring after auto-

claving.

Operating precautions

•Make sure that flow direction is from the inlet in the cap (female

Luer slip fitting) to the outlet in the base (male Luer slip fitting).

NOTICE

Flow in the opposite, unsupported direction

could rupture the membrane.

•Make sure the clamp ring is flushed with the top of the cap

"fingers" before applying pressure so that maximum leak tight-

ness is achieved.

CAUTION

Never operate the holder above 0.34 MPa

(3.4 bar, 50 psi) at 38°C (100°F).

CAUTION

Never use the holder with fluid temperatures

over 140°C (285°F).

Disassembly

To disassemble the product, follow the instructions below.

Step Action

1 Slide the clamp ring away from the "fingers" of the cap.

2 Grip the cap in one hand using the thumb and forefinger. With

the other hand, apply pressure (torque) to the male Luer exten-

sion with the forefinger and thumb until the base snaps loose.

Cleaning

To clean the holder, follow the steps below.

Step Action

1 Completely disassemble the holder.

2 Wash all parts with a suitable solvent (isopropyl alcohol, for

example). If scrubbing is required, use a soft bristle brush.

NOTICE

Do not use acetone as this can harm the

holder.

3 Rinse the disassembled holder in clean water and air dry.

4 Store the disassembled holder in a plastic bag or other clean

receptacle.

Note:

If the holder is pre-loaded and autoclaved, do not store for an

extended period above normal room temperature as, over

time, higher temperatures may cause the membrane to stick to

the o-rings and cause issues during disassembly.

Ordering information

Product code Description Qty/Pk

420100 13 mm Pop-Top Filter Holder 10

Cytiva offers a range of membrane types and pore sizes for use with

the 13 mm Pop-Top Filter holder. For more information on these or

any other Cytiva products, visit cytiva.com.

Give feedback on this document

Visit cytiva.com/techdocfeedback or

scan the QR code.

cytiva.com

Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp. or an

affiliate doing business as Cytiva.

Whatman is a trademark of Global Life Sciences Solutions USA LLC or an affiliate

doing business as Cytiva.

Any other third-party trademarks are the property of their respective owners.

For local office contact information, visit cytiva.com/contact

29690340 AB V:8 03/2023

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