Diagnosys Celeris Manuale utente

CELERIS USER MANUAL

US: Diagnosys LLC USA ● 55 Technology Drive, Suite 100 ● Lowell, MA 01851 USA ● Phone: 978.458.1600 ● Fax: 978.458.1755 ● Email: sales@diagnosysllc.com

UK: Diagnosys LTD UK ● 5 Trust Court, Vision Park ● Histon, Cambridge, CB24 9PW ● Phone/Fax: +44 (0) 1223 520699 ● Email: [email protected]

www.diagnosysllc.com

 
14770-A || ECN # 844 || ECN Date: 20 December 2017 Page 2 of 23 
Celeris User Manual 
Contents 
Introduction...........................................................................................................................................................................................................3 
Contact information.......................................................................................................................................................................................3 
Equipment Covered .......................................................................................................................................................................................3 
Disclaimer ..........................................................................................................................................................................................................4 
Licensing and Copyright ..............................................................................................................................................................................4 
Intended Use.....................................................................................................................................................................................................4 
Technical Specifications and Ratings .....................................................................................................................................................4 
System Specifications...............................................................................................................................................................................4 
Console Buttons and Status Lights .....................................................................................................................................................5 
Dimensions and Weight ..........................................................................................................................................................................5 
Power ..............................................................................................................................................................................................................6 
Input/Output Jacks, Power Button.....................................................................................................................................................6 
Operating Environment...........................................................................................................................................................................6 
Instructions for Use ............................................................................................................................................................................................7 
Setting up for a test ........................................................................................................................................................................................7 
Placing the Electrodes...................................................................................................................................................................................8 
Setup Tips and Tricks................................................................................................................................................................................. 10 
Runtime Menu Controls / Celeris Control Buttons ....................................................................................................................... 12 
Running a Test............................................................................................................................................................................................... 14 
In-test Troubleshooting ............................................................................................................................................................................ 14 
Printing, Saving, and Exporting Data................................................................................................................................................... 16 
Loading Saved Tests ................................................................................................................................................................................... 17 
Creating and Modifying Protocols ........................................................................................................................................................ 18 
Calibration, Cleaning, Disinfection, and Storage............................................................................................................................. 18 
Local Configuration Settings ................................................................................................................................................................... 19 
Tips and Tricks.............................................................................................................................................................................................. 21 
 

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Celeris User Manual

Introduction

The Celeris system uses hardware and software developed entirely by Diagnosys LLC to provide a compact,

transportable system for recording any conceivable ERG or VEP test. Diagnosys’ industry-leading, patent-

pending combined stimulator-electrodes are capable of performing any electrophysiology test, even those

previously possible with only custom-built lab equipment such as Scotopic Threshold responses, intensity

series, Photopic Negative ERGs, or pattern stimuli.

Contact information

Office

Diagnosys US

Diagnosys UK, Ltd

Address

55 Technology Drive, Suite 100

Lowell, MA 01851 USA

5 Trust Court, Chivers Way, Vision Park

Histon, Cambridge CB24 9PW UK

Tel:

+1 (978) 458-1600 (main)

+1 (747) 600-2297 (support)

+ 44 0 1223 520 699 (main)

Email:

mail@diagnosysllc.com (main)

support@diagnosysllc.com (support)

mail@diagnosysuk.co.uk (main)

Hours:

Monday through Friday

9:00 AM –5:00 PM (ET)

Closed Saturday, Sunday, and all major holidays

Monday through Friday

9:00 AM –6:00 PM (GMT)

Closed Saturday, Sunday, and all major holidays

Web:

www.diagnosysllc.com

Diagnosys UK, LTD, is the single authorized representative in the EU for all Diagnosys devices.

Equipment Covered

D430 Celeris High Throughput Testing System with Computer

D431 Celeris Full-field stimulators

D432 Celeris PERG Stimulator

D239 Platinum Needle Electrodes

D166 Stainless Steel Needle Electrodes

Covers software Version 6+

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Disclaimer

Information in this manual is subject to change without notice and does not represent a commitment on the part

of Diagnosys LLC nor can it be held responsible for any omissions or errors herein.

Diagnosys LLC specifically disclaims any warranties expressed or implied about the fitness of this system for any

practical purpose and in no event shall be liable for any loss of profit or other commercial damage including, but

not limited to, special, incidental, consequential, or other damages.

Licensing and Copyright

No part of this document may be copied or reproduced, stored in a retrieval system or transmitted in any form

by any means, electronic, photocopying, mechanical, recording, or otherwise without the prior written

permission of Diagnosys LLC.

Additional copies of this manual may be obtained from Diagnosys LLC.

The Diagnosys Electrophysiology System software is licensed, not sold.

You may install and use one copy of the Diagnosys Electrophysiology System software on a single Celeris System

console. You may not install it on any additional consoles or computers. You may make one copy of the

Diagnosys software solely for backup purposes.

Software licenses for use on additional computers or networks may be obtained from Diagnosys LLC.

All trademarks acknowledged.

Intended Use

The Celeris Ophthalmic Electrophysiology System is a visual electrophysiology system that is intended to be

used by qualified graduate and post-graduate level research personnel or trained technicians. The Celeris

functions by flashing lights or patterns of light at the eye and recording the electrical response. Ophthalmic

electrophysiology provides the only objective measure of visual function.

Technical Specifications and Ratings

System Specifications

Note: See product brochure for updated system specifications

Testing speed

Test 5-10 rodents per hour, depending upon protocol length

Animal setup time

Seconds

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Test species

Mice, rats, gerbils and guinea pigs

Amplifier

•2-channel, fully differential, 32-bit amplifiers have 1 nV resolution over a

4V input range, which provides the highest resolution in the industry

•Ultra-low noise

•5V input range, ensures amplifier will not saturate

•Common mode rejection ratio (CMMR): >100 dB

Rodent platform heater

Temperature: 99°F / 37° C, ± 1°C

Overhead safety light

Red (670 nm) and infrared (940 nm) LEDs

Data reporting

Exports natively to .csv, .txt, or to clipboard for easy import into data processing

software such as Excel, SIGMAPLOT, or MATLAB

Platform cleaning

Flat tabletop covered with waterproof membrane makes cleanup simple and easy

Camera option

Infrared camera available

Protocols supported

Photopic and Scotopic ERG testing

Scotopic Threshold Testing

Flash VEP

Pattern ERG

Stimulator options

Bright: .0003 cd.s/m2through 700 cd.s/m2(extended range available)

Dim: 10-7 cd.s/m2through 10-1 cd.s/m2

Computer operating

system

Windows 10 Professional

Console Buttons and Status Lights

A: Amplifier inputs

B: Run

C: Pause/Preview

D: Stop/Check Impedance

E: Toggle Safety Light/Set Safety Light Intensity

F: Warming Table Power Indicator

G: Warming Table Below Temperature Indicator Light

H: Warming Table Above Temperature Indicator Light (error state)

Dimensions and Weight

Celeris (height to table top): 15.7 x 6.9 x 5.3 inches (399 x 175 x 135 mm)

Celeris heating table: 6.1 x 4.0 inches (155 x 102 mm)

Celeris weight: 10 lbs (4.5 kg)

Figure 1 - Console control buttons and status lights

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Power

Power requirement: 110 V 0- 240 V AC, 50/60 Hz

Rating: Input 85 –264 V AC, 50/60 Hz

Power consumption in use: 24 W

Input/Output Jacks, Power Button

Custom HDMI-connector pattern generator ports x2 (①,②)

Proprietary Ultimate 7-pin Full-field Stimulator jacks x2 (③,⑤)

USB-A power-only output jacks x2 (④)

Proprietary 12V power input (A)

Main power button (B)

Ground port (C)

High-speed (USB2.0) USB-B I/O (D)

DIN4 jack for gooseneck red LED light (E)

Output rating:

USB 1: 5V, 500 mA MAX

USB 2: 5V, 500 mA MAX

DIN4: 12V, .61A MAX

Operating Environment

The system is designed to be operated in a research laboratory under normal ambient temperature and

humidity conditions.

Although no special electrical shielding is required, the Celeris should not be placed in an electrically noisy

environment; large machinery such as cage washers, heavy-duty refrigeration equipment, or elevator motors

may introduce electrical interference into the signal.

You will need all other facilities normally required for visual electrophysiology testing, such as access to running

water and the ability to control room lighting.

Figure 2 - Celeris connections, left front panel

Figure 3 - Celeris connections, back panel

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Instructions for Use

Setting up for a test

Animal Preparation

This should be done before running a test.

Note: this protocol should be performed in accordance with your institution’s Animal Care and Use Committee

regulations, and may be subject to institutional approval.

If any part of the testing will be done in Scotopic (dark-adapted) conditions, the animal should be left in a light-

proof dark room overnight. Be sure to review your institution’s policy regarding overnight housing in advance.

1. Anesthetize the animal. The most common anesthetic for rodent electrophysiology is an intraperitoneal

(IP) injection of ketamine and xylazine, as some gas anesthetics have been found to affect the ERG

response. When using the ketamine-xylazine cocktail, it is important to weigh the animal first so as to

calculate the correct dose. Improper dosing can lead to the death of the animal, or the animal failing to

become fully anesthetized.

2. Maximally dilate the animal’s pupil. Typically a combination of Phenylephrine HCl and either

Tropicamide or Atropine is used. Allow the drops to sit on the eyes for approximately 2 minutes before

wicking excess fluid from the eye in order to prevent inadvertent aspiration. Hydrating drops should

now be applied to the eye to prevent corneal drying and subsequent cataracts. A .3% hypromellose

lubricating gel solution is best; saline or artificial tears will need frequent reapplication to keep the eye

hydrated.

3. Optional –you may wish to trim the whiskers. This is not necessary but may make it easier to place

corneal electrodes, particularly the pattern stimulator electrode.

Environmental Preparation

Cover or turn off any light sources in the room. Light sources covered with red filters may be used but may affect

dark adaptation, due to the wide passband of many filters. The room should be darkened. Ensure that the Celeris

is turned on and that the heating pad has been permitted to come up to temperature. A recovery area for

animals recovering from anesthesia should be prepared in advance.

Software Preparation

From the launch page of the software, select the correct Examiner from the dropdown (for further information

on adding or modifying examiner names to the software, see Local Configuration Settings) and press the Start

button.

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Figure 4 –Launch Page Figure 5 –Subject Selection Page

This will lead you to the subject selection page. By default, each ‘patient’ represents one animal. Default field

names for each animal subject are Animal Number, Study Name, DOB, Gender, and Cage Number. For further

information on customizing field names, see Local Configuration Settings.

Create a new subject by pressing ‘New’ and inputting identifying fields, or searching for and selecting an existing

subject. When the correct subject is selected, press Protocols to advance to the protocol selection page.

Select the test you wish to run from the list. For additional information on customizing test protocols, see

Creating and Modifying Protocols. When you have selected the test protocol, press Run to enter the runtime

menu.

Diagnosys-supplied protocols will display text prior to entering the runtime menu explaining how to set up the

animal.

Placing the Electrodes

Protocols labelled TOUCH/TOUCH ™

TOUCH/TOUCH protocols are protocols that take advantage of the patent-pending stimulator/electrode combo

unique to the Celeris. TOUCH/TOUCH protocols stimulate one eye at a time and use the fellow, unstimulated eye

as the reference.

To set up an animal for a TOUCH/TOUCH protocol, place a small amount of

hypromellose solution on the cup of each electrode, then place an electrode

on each cornea. If you will be using gas anesthetic, make sure that the

animal is positioned securely in the nose cone prior to placing the electrodes

on the cornea. Figures 7 and 8 on the following page show mice with a

TOUCH/TOUCH electrode setup. Note that the nose cone for gas anesthetics is

an optional component. Note also that a third ground electrode is optional in

this setup. See figure 6 at right for Channel labeling guide.

Figure 6 - Channel labeling guide

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Figure 7 - Channel labeling guide

Figure 8 - The TOUCH/TOUCH Setup Figure 9 - The TOUCH/TOUCH Setup with gas nose cone

When plugging a TOUCH/TOUCH protocol into the Celeris, imagine bisecting both the rodent and the Celeris

along the midline of the animal, as seen in Figure 8 below. Every plug/input on the right side of the animal

should stay on the right; every plug/input on the left side of the animal should stay on the left. See Figure 8

below for how the electrodes should be plugged into the amplifier for a TOUCH/TOUCH protocol. The right eye

should be plugged into Channel 1+; the left eye should be plugged into Channel 1-. If a ground is used, it should

be plugged into the GND input ( ).

Figure 10 - Amplifier Setup of a TOUCH/TOUCHTM Protocol

The electrode/stimulator contacting the animal’s right eye (green) is

plugged into the amplifier input 1+ (on the animal’s right) and the

stimulator port to the animal’s right. The electrode/stimulator

contacting the animal’s left eye (blue), is plugged into 1- (the channel 1

input closest to the animal’s left), and the stimulator port to the

animal’s left.

Protocols labelled ACTIVE/REF/GND

ACTIVE/REF/GND protocols are traditional protocols that use binocular stimulation, a shared reference

electrode, and a ground electrode to reduce or eliminate mains interference. Typically reference electrodes are

placed either subcutaneously in the forehead area, or a bite bar placed in the mouth. Ground electrodes are

typically needle electrodes placed subcutaneously in the tail or the hindquarters near the tail. The right eye will

be plugged into Channel 1+, the left eye will be plugged into Channel 2+, the shared reference will be plugged

into both Channel 1- and Channel 2-, and the Ground into the ground input.

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Simultaneous ERG and VEP Protocol

The simultaneous ERG and VEP protocol supplied by Diagnosys uses input channel 1 for the corneal/ERG

recording and input channel 2 as the VEP/cortical recording.

Figure 11 –Setup for combined ERG/VEP. GND in tail (not pictured) Figure 12 - Layout for ERG/VEP protocol Figure 13 - alternate

view of Flash VEP setup

The animal’s right eye stimulator/electrode is plugged into Channel 1+ (indicated in yellow in Figure 10 above).

The animal’s left eye stimulator/electrode plugs into Channel 1- (red). A VEP reference needle electrode should

be placed in the snout or cheek (as seen Figure 11 above). The plug for the VEP reference should go into channel

2- (indicated in blue in Figure 10 above). The active VEP electrode should be at the midline at the back of the

head, and is plugged into 2+ (indicated in purple). The final ground electrode is placed in the tail or hindquarters

near the tail, and is plugged into the input circled in green in Figure 10. (See Figures 9 - 11).

Setup Tips and Tricks

Ground electrode

•The ground electrode is a non-recording electrode that is used to prevent electromagnetic noise (for

example, from power cables) from interfering with the signal. Common placement sites include the tail,

or the hindquarters near the tail. While there is no electrophysiological advantage to either site,

Diagnosys recommends placing the electrode on the hindquarters near the tail, as the skin of the tail is

typically tougher and will blunt an electrode more quickly.

•The Celeris has been hardened against electromagnetic interference, and you may find that adding a

ground electrode is not necessary to protecting against mains interference, particularly in

TOUCH/TOUCH protocols. TOUCH/TOUCH protocols by default assume no ground electrode is placed.

•In addition to preventing electromagnetic noise from interfering with the signal, the ground electrode

permits the impedance of each active electrode to be assessed independently. Without a ground, only

the average of the impedances of the two electrodes can be determined and therefore both eyes will

always show the same value.

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