Diamond SureFlow Manuale utente

diamond SureFlow,.

1. Safety Recommendations

Please read the instruction manual carefully before using the dispenser. Although we have

tried to cover all safety requirements, it is impossible for this manual to prevent all possible

safety hazards. It is the responsibility of the user to establish their own safety requirements and

to determine the necessary precautions prior to using the dispenser.

Please read and follow the following safety recommendations:

•Fully read and adhere to all recommendations and instructions in this manual

•Use protective clothing, eye protection and gloves when working with hazardous liquids.

•Do not dispense inflammable reagents into plastic vessels that can potentially generate a

static charge.

•This device is not suitable for use with the following:

Concentrated {hydrochloric acid, fluorinated hydrocarbons, saline solutions, nitric

acid), highly concentrated alkaline solutions and crystallizing solutions.

Incompatible liquids that attack FEP, PFA, PTFE, borosilicate glass, AbO3, organic

solvents, trifluoroacetic acid, explosive liquids, fuming acids, tetrahydrofuran, suspension {e.g.

charcoal) as solid particles.

•Handle the dispenser carefully to avoid any accidents or spills during usage.

•The nozzle should always point away from the user while dispensing. Avoid splashes.

•Always use suitable vessels for dispensing liquids.

•Never push down on the piston while the nozzle cap is on.

•Clean the discharge tube regularly.

•Do not use excess pressure on the piston while aspirating or dispensing or the glass tube

may break inside. Follow the troubleshooting guide if you experience any difficulty moving

the piston up and down.

•Only use Globe Scientific accessories.

2. Operating Conditions

•+ 15

C to +40

C {59

F to 104

F) for bottle top dispenser and reagent

•Vapor pressure upto 500 mbar.

•Densityupto2.2g/cm

•Kinematic viscosity upto 500 mm

/second

{dynamic viscosity [mPas]=Kinematic Viscosity [mm

/s] x density [g/cm

3] )

3. Package Contents

Description

-Dispenser

-Telescopic filling tube

-Recirculation tube*

- Bottle adapters** {Adapter thread sizes : 28, 38, 40 & 45mm)

-Calibration Tool

-Service tool

-Connector {only for 100 ml) {Thread size: 40 / 32 mm)

-User manual

-Certificate of Conformity including calibration certificate

-Warranty card

*This accessory is included in recirculating models only

** 32mm thread size is the standard thread built into each dispenser. No adapter is required for this

thread type

4. Overview

®------1] 11

----=--,

@r---CD

1. Cap

Head

3. Main Body

4. Pointer

5. Volume Knob

6. Cylinder Sleeve

7. Cylinder

8. Piston

9. Main Valve

10. Valve lever (piston)

11. Recirculation Valve

12. Spout

13. Front Cover

14. Nozzle

15. Nozzle Cap

16. Air Plug

17. Adapter

18. Telescopic Filling Tube

19. Recirculation tube

20. lnnervalve

21. Fillingvalve

�• Note : Recirculation items are only included in recirculating dispenser models.

5. Assembly

1. Mounting the telescopic fill

ng tube/

recirculation tube

Adjust length of the telescop

ng filling tube to the bottle

height and attach it careful

y to the cente

of the dispenser.

If a dispense

th recirculation is used, the additional

recirculation tube should also be

nsta ll ed. Install this tube

th the cu

ved portion po

nti ng outward / towards the

nozzle {Fig.1)

2. Mounting the dispenser on a bottle

Carefully screw the dispenser assembly {GL 32 threads)

onto the reagent bottle and ensure that the nozzle is

aligned with the graduation marks on the main body of the

bottle.

@-Note : For bottles with different thread sizes, please select

the appropriate adapter.

The adapters supplied with the dispenser are made of

polypropylene (PP), and can only be used with reagents

which do not attack PP.

WARNING

Always wear protective gloves when touching the

dispenser or the bottle, especially when using dangerous

liquids.

When mounted to a reagent bottle, always carry the

dispenser vertically as shown in Fig. 3

Fig. 2

";}

}

I{.;

-r::

er:,

5CC

-- >

Fig. 3

6. Priming

6.1 Bottle Top Dispenser with recirculation valve mechanism

A_ WARNING

The nozzle should always point away from the user. Please ensure that the piston is never pressed

down when the nozzle cap is mounted to the dispenser tube. Avoid splashing.

Please follow the steps below to prime the dispenser:

1. Turn the lever to recirculation mode. (Fig. S)

2. Slowly pull up the piston and push it down smoothly to the end stop. Repeat this until

air bubbles disappear from the cylinder. (Fig. 6)

3. Turn the lever back to it's original position for dispensing.

Fig.5

Adjusting recirculation lever

6.2 Bottle Top Dispenser without recirculation valve mechanism

A_ WARNING

The nozzle should always point away from the user. Please ensure that the piston is never pressed

down when the nozzle cap is mounted to the dispenser tube. Avoid splashing.

Please follow the steps below to prime the dispenser:

1. Place a vessel below the nozzle.

2. Hold the spout and carefully remove the

nozzle cap. (Fig. 7)

3. Slowly pull up the piston and push it down

smoothly to the end stop. Repeat this until air

bubbles disappear from the cylinder. (Fig. 6)

7. Operating Instructions

7.1 Setting the volume

1) Turn the volume setting knob counterclockwise to unlock.

2) Slide the knob up or down to the desired volume.

3) Align the pointer on the volume setting knob to the desired volume and carefully turn

the knob clockwise to lock at that setting.

Your dispenser is now ready to work at your desired volume. (Fig. 8)

7 .2 Dispensing

&WARNING

Please use caution when dispensing dangerous reagents.

Fig. 8

1. Please follow the priming process (Section 6) before dispensing.

2. Ensure recirculation lever is aligned with nozzle.

3. Make certain a vessel is below the nozzle at all times while dispensing.

4. Set your desired volume (as shown in section 7.1- Setting the Volume).

5. Gently pull the piston up until reaching the upper end stop, then push the

piston down smoothly until reaching the lower end stop.

6. When completed, gently touch the end of nozzle against the inner wall of the

receiving vessel (for touch-off). Repeat this process for each of the dispensing

cycles required.

7. Reattach the nozzle cap to the nozzle when finished.

• Note : Do not use excessive force while pushing down the piston. If you feel the piston is jammed,

•please follow the troubleshooting guide (Section 12)

Please ensure that the piston is at lower stop before reattaching the nozzle cap.

The user may detect a slight sound during aspiration when moving the dispenser; please note this is

normal (due to the ball design used in the piston/ valve).

8. Cleaning

Please make sure to clean the dispenser regularly to ensure good operation.

Cleaning should be performed if any of the following occur:

•If the piston becomes stuck inside the tube

•Before changing reagents

•Long periods of downtime

•Before autoclaving

•Before any maintenance

• Please make sure to wear suitable eye protection glasses and protective clothing.

Please follow the steps below prior to cleaning:

1. Completely dispense the liquid present inside the dispenser.

2. Mount the dispenser on a bottle filled with deionized water or any other suitable

cleaning agent and rinse the dispenser several times.

3. Pull off the telescopic tube (and recirculation tube if applicable) and clean with

deionized water.

4. For dispensers with recirculation, set the unit to recirculation mode and again rinse the

dispenser several times.

Please follow the steps below to clean the piston and glass barrel:

• This procedure must be followed if the piston is difficult to move or if dispenser is to be autoclaved.

1. Unscrew the head as shown in Fig. 9

2. Take out the piston completely as shown in Fig.10

3. Clean the piston & glass barrel with deionized water and then reassemble in reverse.

Fig. 9

•r -,c, Fig. 10

Disassembly of the valve

1. Put out the Re-circulation tube & telescopic tube (Fig. 11)

2. Use the service tool to unscrew the filling valve and also take out the ball with care. (Fig.12)

3. Use the other side of the service tool to unscrew the inner valve (Fig. 13) and remove it

(Fig.14)

4. After cleaning/replacement, insert inner valve in the same position as before removal.

5. Screw the filling valve first by hand and then tighten it with the service tool.

• If some elastic resistance is present while

aspirating the reagent, then it is possible that

the ball valve may be sticking/stuck.

To correct this, exercise the ball valve using

very light pressure, for example, 200 µI

plastic pipette tip (Fig. 14)

9. Accuracy

Error limits related to the nominal capacity {maximum volume) indicated on the instrument are

obtained when instrument and distilled water are equilibrated at ambient temperature

{20 °C/68°F). The calibration process is performed as per the EN ISO 8655-5 standard with a

completely assembled dispenser and uniform dispensing.

Volume Increment Accuracy(±) % Precision (±) %

(ml) (ml) ±% ±ml ±% ±ml

0.25 -2.5 0.05 0.6 0.015 0.2 0.005

0.5 - 5 0.1 0.5 0.025 0.2 0.01

1-10 0.2 0.5 0.05 0.2 0.02

2.5 -25 0.5 0.5 0.125 0.2 0.05

2.5 -30 0.5 0.5 0.15 0.2 0.06

5 - 50 1.0 0.5 0.25 0.2 0.1

5 - 60 1.0 0.5 0.3 0.2 0.12

10 -100 2.0 0.5 0.5 0.2 0.2

10. Calibration

WARNING

During calibration you must begin at the lowest volume first, ensuring the body of the dispenser is not

moved before you close the cap and then proceed to the medium and high volumes.

Calibration check

1. Please follow the calibration procedures as described in EN ISO 8655-5 (i.e. environmental

conditions, water, balance, etc.).

Indice

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