Diffinity Genomics RapidTip2 Manuale di istruzioni
USER HANDBOOK
User Notice:
For optimal purication results, sharply tap
the RapidTip2 package 2–3 times on a at
surface so that the particles drop to the
bottom of the tips.
Version B (March 2012)
For PCR Purication with
Polymerase Removal
Page 2 www.difnitygenomics.com
Table Of Contents
Kit Contents 3
Storage 3
Product Use Limitations 4
Product Warranty and Satisfaction Guarantee 4
Quality Control 5
Technical Assistance 5
Safety Information (Disposal) 5
Product Specifications 6
Introduction 7
Diffinity Technology Principle 8
Equipment to be Supplied by User 9
Guidelines — Pipetting with the RapidTip2 10
Pipetting Examples 11
RapidTip2 PCR Purification Protocols 12
Quantifying DNA Recovery 13
Troubleshooting Guide 14
Ordering Information 15
Difnity RapidTip2 Page 3
Kit Contents
Diffinity RapidTip2s can be used with universal or Rainin LTS
style pipettes. All kits come with a user handbook.
Cat Number Product Description Contents
RR050-008
RR050-008L*
Diffinity RapidTip2 for
PCR Purification with
Polymerase Removal
1 Pack of 8
RapidTip2s
RR050-048
RR050-048L*
Diffinity RapidTip2 for
PCR Purification with
Polymerase Removal
1 Box of 48
RapidTip2s
RR050-096
RR050-096L*
Diffinity RapidTip2 for
PCR Purification with
Polymerase Removal
1 Box of 96
RapidTip2s
* L — denotes Rainin LTS format pipette tips.
Storage
Diffinity RapidTip2s should be stored in low humidity at room
temperature (15–25 degrees Celsius). Under these conditions,
Diffinity RapidTip2s can be stored and used for up to 12
months without reduction in performance or quality. An
expiration date is on the label of the 48 and 96 tip boxes.
Page 4 www.difnitygenomics.com
Product Use Limitations
Diffinity RapidTip2s are intended for research use only. Diffinity
RapidTip2 is designed for purification of 50 μl PCR solutions
prior to dideoxy (BigDye) reaction for Sanger Sequencing,
restriction digestion, or T-A cloning. RapidTip2 is compatible
with most universal and Rainin LTS pipettors. RapidTip2 is not
compatible with detergents, mineral oil, or ready-to-load style
PCR master mixes that contain a density-increasing compound.
Product Warranty and Satisfaction Guarantee
Diffinity Genomics will guarantee the performance of Diffinity
RapidTip2s for the uses and performance as described in
our product literature. If the product fails to perform to your
satisfaction for any reason other than misuse, we will replace
the product free of charge or refund the purchase price —
whichever you choose. Diffinity Genomics maintains the right
to update or modify the product to enhance its performance. A
copy of our terms and conditions can be found on our web site.
If you have questions about the product or its performance,
please contact Diffinity Genomics customer service at
1-877-362-1812.
Difnity RapidTip2 Page 5
Quality Control
Each lot of Diffinity RapidTip2s is tested against predetermined
specifications to ensure consistent quality.
Technical Assistance
Call 1-877-362-1812 between 8am and 4pm Eastern Time
Monday through Friday or visit www.diffinitygenomics.com for
help with common questions.
Safety Information (Disposal)
Diffinity RapidTip2s may be disposed in regular lab trash. For
more information, please consult the material safety data sheet
(MSDS) available on request by calling Customer Service at
1-877-362-1812.
Page 6 www.difnitygenomics.com
Product Specications
Capacity: PCR Sample Size 50 ±5 microliters
dsDNA Recovery up to 90%
(100bp to 10Kb)
Impurity Removal (dNTP,
primers, primer-dimers)
up to 90%
(less than 40–50 bp)
DNA Polymerase removal
(Taq, Hot-Start, High-
Fidelity)
up to 1:1000 dilution with
no measurable amplification
activity
Diffinity RapidTip2 tips are designed to remove dNTPs,
primers, primer-dimers, and DNA polymerase at typical PCR
concentrations.
Difnity RapidTip2 Page 7
Introduction
Diffinity Genomics has developed an innovative new
technology that removes undesirable impurities from PCR
reactions leaving you with nothing but purified DNA!
This revolutionary technology effectively removes dNTPs,
primers, primer-dimers, and DNA polymerase while providing
up to 90% recovery of pure DNA fragments ranging in length
from 100bp to 10Kb. It dramatically reduces the amount of
time to purify and prepare PCR reactions for use in downstream
applications, such as Sanger sequencing, restriction digestion,
and/or T-A cloning.
The RapidTip family of tips for PCR purification require just
one-tip, one-step, and one-minute for PCR purification!
Page 8 www.difnitygenomics.com
Difnity™ Technology Principle
The Diffinity RapidTip2 contains everything you need for PCR
purification. The tip is filled with our proprietary adsorption
technology, which has a differential affinity for PCR reaction
components. The impurities are removed from the solution as
it enters the pipette tip. Dispensing the solution yields purified,
high quality DNA ready to use for downstream applications.
You won’t need additional equipment, reagents, buffers, or
enzymes — only a pipettor!
Difnity RapidTip2 puries PCR
solutions and removes DNA
polymerase comparably to
popular spin column methods.
The positive control (+) con-
tains polymerase and all PCR
reagents but did not undergo
purication and therefore has
a 600bp band. The control
purication (C) and RapidTip2
purication (R) do not have
bands showing that polymerase
was removed during purica-
tion.
Difnity RapidTip2 effectively
removes dNTPs (not visible on
gel), primers (20bp), and primer-
dimers (50bp). The PCR product
was divided and subjected to PCR
purication with either competitor’s
products (C) or RapidTip2 (RT2: 1,
2, 3). RapidTip2 has high yield: the
PCR amplicon (2Kb) shows no loss
of band intensity after purication. 20bp--
50bp--
2Kb--
U C C 1 2 3
---RT2---
+ -C R
Standard
Hot Start
Proofreading
Difnity RapidTip2 Page 9
Diffinity RapidTip2
purification
improves sequence
quality. Sequence
chromatograms
show improved
peak quality and
lower background in
Diffinity RapidTip2
purified samples
as compared to
unpurified samples.
Equipment to be Supplied by User
• PCR Sample (50 μl ±5 μl)
• Tubes to store purified DNA
• Pipettor — manual or programmable
• Standard pipette tips for liquid transfer
(if necessary)
RapidTip2
unpuried
Page 10 www.difnitygenomics.com
Guidelines — Pipetting with the RapidTip2
Careful pipetting is important to achieve effective purification
with the RapidTip2. The following pipetting guidelines will help
ensure optimal results:
1. Pre-wet the particles
On the first aspirate step, aspirate approximately the first
half of the sample and pause 5 seconds to ensure complete
wetting of the particles before mixing. Then aspirate the rest
of the sample and dispense.
2. Mix effectively
For effective mixing, it is important that the particles
remain in contact with the sample during each aspirate and
dispense cycle.
Aspirate slowly for the first several cycles until the particles
are completely dispersed into the PCR sample.
Avoid long delays between successive aspirate and dispense
cycles that allow the particles to settle out of solution for
more than 1–2 seconds.
3. Maintain tip-to-sample contact
During aspiration, maintain continuous contact between
the end of the pipette tip and your sample to avoid
aspirating air bubbles in the sample.
Indice
Manuali Attrezzature da laboratorio popolari di altre marche
Agilent Technologies
Agilent Technologies 5800 ICP-OES Manuale utente
Endress+Hauser
Endress+Hauser Cleanfit CPA875 Manuale utente
NI
NI PXI-5422 Manuale
Collomix
Collomix Aqix Manuale utente
SPEX SamplePrep
SPEX SamplePrep 6875 Freezer/Mill Series Manuale utente
Ocean Insight
Ocean Insight FLAME-NIR+ Manuale utente
