DINKO Instruments UV/VIS 6000 Manuale utente

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

UV/VIS 6000 Ultraviolet Visible Spectrophotometer

Smart Touch Screen

Reference Manual

Version: March 2021

Mark

c/ Encarnació, 123-125 / 08024-Barcelona

dinter@dinko.es www.dinko.es

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

Dear user:

Thank you for purchasing and using the instrument of shanghai metash

instrument corporation. Please read the manual carefully to enable you to

know the performance of the instrument as soon as possible and to master

its using method.

The copyright of the manual belongs to shanghai metash instrument

corporation. Without the permission of our company, deleting and copying

of the contents in any form is forbad for anybody.

It is possible to modify the contents of the manual for the need of improving.

If any modification happens, please forgive us no further notice.

You can log on to our official website http://www.dinko.es for updates, or

call our hotline +34 93 284 69 62, to obtain the relevant advice and help.

Although we have checked this manual carefully, some mistakes cannot be

avoided, if any inconvenience is caused, please understand. If you find

some mistakes or have better suggestions, you are welcome to get in touch

with us to enhance our further improvement. we will actively adopt you

rational suggestions to ensure that our instrument will be improved

continually to meet your needs.

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

Contents

1. Summary............................................................................................5

1.1 Working Principle .....................................................................5

1.2 Instrument Characteristics.........................................................6

1.3 Application Field.......................................................................7

1.4 Technical Parameters.................................................................8

1.5 Symbols and Prompt Notes .......................................................8

1.6 Brief Introduction of System and Structure ...............................9

2. Installation .......................................................................................10

2.1 Unpacking...............................................................................10

2.2 Installation Condition..............................................................10

2.3 Instrument Installation.............................................................12

3. Operating the Instrument..................................................................12

3.1 Power on Self-test ...................................................................12

3.2 Photometric Analysis...............................................................16

3.2.1 Photometric analysis.......................................................17

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

3.2.2 Data processing ..............................................................18

3.3 Quantitative Analysis ..............................................................20

3.3.1 Standard curve test .........................................................21

3.3.2 Application of coefficient method .................................................29

3.3.3 Setting concentration unit..............................................................31

3.4 Spectral Scanning.......................................................................32

3.4.1 Wavelength scan................................................................34

3.4.2 Processing data................................................................374

3.5 Kinetic Analysis.......................................................................429

3.5.1 Kinetic analysis.................................................................41

3.5.2 Processing data................................................................462

3.6 System Settings........................................................................517

3.6.1 Lamps management............................................................ 48

3.6.2 Time Settings.....................................................................50

3.6.3 Dark current calibration...................................................561

3.6.4 Wavelength calibration....................................................572

3.6.5 System debugs.................................................................583

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

3.6.6 Restoring defaults............................................................584

4. Routine maintenance........................................................................606

5. Troubleshooting ...............................................................................629

1.Summary

1.1 Working Principle

The working principle of spectrophotometer is mainly based on Lambert -

Bill law. When a bundle of monochromatic light passes through a uniform

colored solution, the absorbance of the solution is proportional to the

product of the concentration of the solution and the optical path,

which is

the true physical meaning of Lambert Bill's law. The law is the basis of

quantitative analysis of photometric analysis, and also the basic principle

of UV-Vis spectrophotometer. The formula for Lambert-Bill law is

expressed as follows:

A=k b C

A--The absorbance of the measured substance solution.

k-- Absorption coefficient of solution.

b--

The thickness of the liquid layer (the optical path of the cuvette or

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

absorption pool), cm.

C—The concentration of the measured substance solution.

The premise of satisfying the Lambert Bill law is three hypotheses: First, the

incident light is monochromatic light. Second, the incident light is parallel light.

Third, light-absorbing particles are independent of each other, regardless of

quantity and variety. In addition, the deviation of the law is affected by sample

treatment, the optical path of measure, stray light, noise, the spectral bandwidth,

the chemical factors and other factors. The abovefactors need be taken into account

in the routine tests.

1.2 Instrument Characteristics

The characteristics of UV/VIS 6000 Spectrophotometer are the following:

The stray light is low and resolution is high in optical system, so the

stability and reproducibility are excellent and the readings are accurate.

The design is scientific, new technologies are used to combine the light,

instrument, electricity with computer to make the stability indicators of

the instrument close to or reach the high-level of UVspectrophotometer.

7 inch TFT capacitive touch screen, the touch point is more accurate,

sensitivity is more higher and stability is more better.

High definition resolution (800 * 480), fast running, large capacity for

picture.

Interactive and friendly user interface, and it is easy and fast to be used.

System settings function is powerful, the measurement function of

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

photometric analysis, quantitative analysis, wavelength scan and

kinetic analysis can be realized without online operation.

Large data storage: 30 wavelength scan files, 20 kinetic measurement

files, multiple sets of measurement data and standard curves.

Data retrieval and atlas zooming function.

The universal printer (inkjet or laser) can be connected with the

instrument, A4 report output can be completed directly and the report

is more clear and beautiful.

USB transfer function can be achieved; data storage and reading is

more flexible.

1.3 Application Field

Ultraviolet visible spectrophotometer can be widely used in the fields of

pharmacy, medical and health services, chemical engineering, energy,

machinery, metallurgy, environmental protection, geology, food, biology,

materials, agriculture, forestry, fisheries and other industries, and plays an

important role in teaching and research of higher education, measurement

science and research institutes, quality control in the production, raw

materials and product analysis, and is commonly used in physicochemical

laboratory. UV/VIS 6000 is a UV spectrophotometer with touch screen.

The instrument is stable, accurate and powerful and has obvious

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

advantages in the fields of scientific research and quality control.

1.4 Technical Parameters

Type UV/VIS 6

Wavelength range (nm) 19 -11

Spectral bandwidth (nm) 1,8 nm

Wavelength accuracy (nm) ± .5

Wavelength repeatability

(nm) ≤ .2

Photometric range -2 %T、- .3-3A、-9999C

Photometric accuracy ± .4 %T

Stray light ≤ . 7 %T

Size (mm) 46 ×38 ×18

1.5 Symbols and Prompt Notes

Power supply,

beware of electric shock.

: Heat source, burn carefully.

: UV light source, be careful radiation.

: Notice.

: Special note.

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

1.6 Brief Introduction of System and Structure

1、The shape and structure of the instrument

The shape and structure of the instrument is shown in figure 1:

1 2 3

1--Hand pull rod 2—Sample room 3--Touch screen

Figure 1 The shape of UV/VIS 6000

The back of the UV/VIS 6000 instrument is shown in figure 2:

1—Power switch 2-- Power supply socket 3-- Cooling vent

4-- Printer port

DINKO

INSTRUMENTS

User’s Manual UV/VIS 6000

Figure 2 The back of UV/VIS 6000

2.Installation

Please read the contents of this chapter carefully before installation.

2.1 Unpacking

Please check to make sure that the instrument external packing is intact and

has no opened marks before unpacking. After unpacking, please inspect to

make sure the instrument has no damage and the accessories is complete

by referring to the packing list in the manual (page thirty-fifth of this

manual). If you have any question, please consult the dealer or

manufacturer. If you find any damage or loss in the package, please inform

the manufacturer immediately.

2.2 Installation Condition

The following requirements should be met for the installation of the

instrument:

1) The requirements of temperature and humidity:

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