ETT Olfactometer 2 Manuale utente

ETT Olfactometer & ETTDirectControl

The First Paradigm

Manual and Tutorial

Revision 2021.1

 
3 
© Emerging Tech Trans, LLC 
 
Table of Contents 
The First Paradigm...................................................................................................................................... 5 
Warnings................................................................................................................................................. 5 
Software Installation (ETTDirectControl) ................................................................................................ 5 
Access to our installer......................................................................................................................... 5 
Download............................................................................................................................................ 6 
Software overview................................................................................................................................ 10 
Manual Mode.................................................................................................................................... 10 
Paradigm Designer............................................................................................................................ 10 
Updater / Settings............................................................................................................................. 12 
Help................................................................................................................................................... 14 
Connecting the Device.......................................................................................................................... 16 
Before the first power up.................................................................................................................. 16 
ETT Olfactometer 2........................................................................................................................... 17 
ETT Olfactometer S & ETT Olfactometer C........................................................................................ 19 
Prerequisites......................................................................................................................................... 21 
The paradigm.................................................................................................................................... 21 
Preparing the odorants..................................................................................................................... 21 
Connecting the tubing....................................................................................................................... 22 
Flow rate adjustments ...................................................................................................................... 24 
Creating a simple paradigm.................................................................................................................. 25 
Creating a new paradigm.................................................................................................................. 25 
Configuring an olfactory condition.................................................................................................... 27 
Configuring the visual component.................................................................................................... 29 
Managing, cloning and adjusting groups........................................................................................... 31 
Assembling paradigm........................................................................................................................ 32 
Labeling conditions for data analysis ................................................................................................ 32 
Group randomizer............................................................................................................................. 32 
Triggered start .................................................................................................................................. 33 
Recording the respiratory signal ....................................................................................................... 33 
Test run............................................................................................................................................. 33 

 
4 
© Emerging Tech Trans, LLC 
Inspecting the data file ..................................................................................................................... 36 
Using Triggers in ETTDirectControl ....................................................................................................... 39 
TTL IN (Externally triggered start) ..................................................................................................... 39 
Respiratory Trigger............................................................................................................................ 40 
Beyond basics ................................................................................................................................... 42 
Cleaning the odorant carrier..................................................................................................................... 46 
Before we start:.................................................................................................................................... 46 
Cleaning the carrier top........................................................................................................................ 46 
Cleaning the bottles.............................................................................................................................. 50 
Cleaning the tubing............................................................................................................................... 50 
Odorant flow setup................................................................................................................................... 52 
Carrier Setup......................................................................................................................................... 52 
Check valve layout in the odorant carrier............................................................................................. 52 
Typical air flow configuations ............................................................................................................... 53 
Configuration for multiple channels at once......................................................................................... 55 
Setting up multiple channels within ETTDirectControl ......................................................................... 56 
ETT EEG Trigger Interface.......................................................................................................................... 57 
Hardware setup .................................................................................................................................... 57 
System overview............................................................................................................................... 57 
Software Setup ..................................................................................................................................... 58 
Prerequisites..................................................................................................................................... 58 
EGI NetStation................................................................................................................................... 58 
ETT Direct Control............................................................................................................................. 60 
 
 

The First Paradigm

Warnings

1. This device is for research use only!

2. The main device / the actual ETT Olfactometer is not MRI compatible.

3. Only the ETT Odorant carrier, tubing, respiratory belt and the applicator/nose piece are MRI

compatible and thus allowed into the magnet room.

4. Feed all tubing through an appropriately sized wave guide to avoid damage to it.

Software Installation (ETTDirectControl)

Access to our installer

For registered users, we are offering all our software as a download on our website

(emergingtechtrans.com). Access will be provided during the purchase of the device via email contact

with our support. Additional accounts can be created at any time upon request to

support@emergingtechtrans.com.

With access to our support section (Figure 1) there are two to three different versions of

ETTDirectControl accessible. Depending on your request, we can unlock specific preview version access.

All installer packages describe supported operating systems and contain all necessary dependencies in

the package. Should it be required, we are also providing links to the driver and library packages below

separately.

It should be noted that the previous stable version, while still actively maintained is limited in its

features to support our users that are still using Windows XP. To use all new features, download the

current stable / release version. Please look at the Meaning of Update Rings on page 13, to find out

more about each version.

Figure 1 - Software support section on emergingtechtrans.com

Download

Once the fitting version / update track was chosen, a click on the download button will download the

software. All installers automatically contain 32/64bit compatible installers.

Figure 2 –Double-click on the downloaded executable to initiate the setup process

Since ETTDirectControl requires device drivers, it will require to be installed with Administrator

privileges (Figure 3).

Figure 3 - For drivers and device access ETTDirectControl requires administrator privileges

Once the necessary privileges are granted the installer will guide through each step. Please adjust the

settings appropriately and click Next each time to progress (Figure 4). If multiple versions of

ETTDirectControl are installed on the same machine, change the installation path. Make sure to check to

install both pre-requisites:

1. Microsoft .NET Framework 4

2. FTDI USB drivers

Under some circumstances (failed to install .NET Framework) these options must be unchecked and

installed separately. Follow Figure 4 through Figure 10 to complete the installation. At last the installer

will offer to run ETTDirectControl. Yet again administrator privileges must be granted.

Figure 4 - First page of installer - after selecting the appropriate settings, click next to progress.

Figure 5 - Adjust the installation path if necessary. Select .NET and USB drivers as they are needed for ETTDirectControl to

operate (any needed extras will be automatically checked).

Figure 6 - Define path of installation

Figure 7 - Set Start Menu folder

Figure 8 - Adjust icon settings as necessary.

Figure 9 - Trigger actual installation

Figure 10 - Step through FTDI USB driver and .NET Framework 4 installers

After successfully completing the installation and first starting the application, it is a good time to first

plug the ETT Olfactometer into the USB port (consult Connecting the Device on page 16 if necessary).

The first start might spawn a Windows driver initialization and could take a moment. Once completed,

the device should be successfully recognized as shown in Figure 11. Under some circumstances it may be

required to reboot the PC before the device can be recognized.

Software overview

Manual Mode

The Manual Mode tab in ETTDirectControl will list any connected device that is currently compatible to

this software. Depending on the type of device and specific features, the interface for the device will

adjust. For our Olfactometers a list of channels / valves will be presented in the form of clickable

buttons. A gray button means the valve is currently closed, while blue color represents currently opened

valves (Figure 11Figure 15). The number of listed buttons will adjust with the number of valves that your

specific Olfactometer has.

Figure 11 - Manual Mode with connected ETT Olfactometer 2 with active simulated respiratory signal. Valve 3 with the blue

background color is opened.

Features custom to each device and their current state will be represented below. In the depicted case

of an ETT Olfactometer 2 these are TTL Trigger (for external triggers) and control of the Respiration

sensor system. If the latter is active, a small preview plot of the subjects’ respiratory patterns will be

depicted next to these.

This mode is meant to help design and debug paradigms, where it can be useful to manually open and

close valves to test odorant concentrations and stimulus durations. It can also help to explore the

influence of connected sensors that are set around trigger conditions used in the paradigm. Prior to

running an experiment with a human subject, it is advisable to check in this view, if all systems are active

and working.

Paradigm Designer

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