Fluidigm Juno Manuale utente
PN 100-7070 J2
Juno System
User Guide
Juno System: User Guide
2
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For Research Use Only. Not for use in diagnostic procedures.
Information in this publication is subject to change without notice. It is
Fluidigm policy to improve products as new techniques and components
become available. Therefore, Fluidigm reserves the right to change
specifications at any time. Every effort has been made to avoid errors in
the text, diagrams, illustrations, figures, and screen captures. However,
Fluidigm assumes no responsibility for any errors or omissions. In no
event shall Fluidigm be liable for any damages in connection with or
arising from the use of this publication.
Patent and Limited License Information
Fluidigm products are covered by issued and pending patents in the
United States and other countries. Patent and limited license information
is available at fluidigm.com/legalnotices.
Limited Use License to Perform Preamplification with Fluidigm IFCs
A license to use Thermo Fisher Scientific’s patented preamplification
method workflows involving a Fluidigm integrated fluidic circuit (IFC) can
be obtained (i) with purchase of a Fluidigm IFC from Fluidigm Corporation
or (ii) by a separate license from Thermo Fisher Scientific. For licensing
information, contact outlicensing@lifetech.com.
Limited Digital PCR License
A license to use Thermo Fisher Scientific’s patented digital PCR method in
all fields other than in the Sequencing Field, the Mass Spectrometry Field,
and the Prenatal Field in workflows involving a Fluidigm IFC can be
obtained (i) with purchase of a Fluidigm IFC from Fluidigm Corporation or
(ii) by a separate license from Thermo Fisher Scientific. For licensing
information, contact outlicensing@lifetech.com.
Trademarks
Fluidigm, the Fluidigm logo, Biomark, Digital Array, Dynamic Array, EP1,
Flex Six, Juno, qdPCR 37K, and SNP Type are trademarks and/or
registered trademarks of Fluidigm Corporation in the United States and/or
other countries. All other trademarks are the sole property of their
respective owners.
For EU's WEEE directive information, go to fluidigm.com/compliance.
© 2018 Fluidigm Corporation. All rights reserved. 12/2018
Juno System: User Guide
Contents
3
Contents
About This Guide 5
Purpose 5
Safety Alerts for Chemicals 5
Safety Alerts for Instruments 5
Safety Data Sheets 6
Chapter 1: Introducing the Juno System 7
The Juno System 7
Consumables Ordering 7
Components of the Juno System 8
Interface Plates and IFCs 10
System Software 10
Scripts 11
Basic Scripts 11
Advanced Scripts 14
Components Included in Shipping Box 15
Optional Components 15
System Functions 16
Air Options 16
Regulatory Compliance 16
General Regulations and Requirements 16
Harmonized Standards 16
Conformity Symbols on the Instrument 17
Chapter 2: Getting Started with Juno 18
Start the Juno System 18
How to Use the Juno Touchscreen 20
Start a Run 20
Load an IFC 20
Select a Script 23
Schedule the Harvest with the LP 192.24 IFC or
LP 48.48 IFC 24
Run a Script 24
Switch an Interface Plate 26
Install an Interface Plate 29
View System Information 32
Export a Log 33
Shut Down the System and Then the
Instrument 34
Chapter 3: Using Advanced Features
of Juno 36
Log In 36
Log Out 36
Manage Scripts 37
Enable Advanced Scripts 37
Export Scripts to a USB 37
Install Scripts from a USB 38
Modify Scripts 39
To View the Scripts to Modify 39
To Change General Properties of a Script 40
To Change Thermal Properties of a Script 40
To Remove a Script 42
Manage Users 42
Change the Date and Time 44
Update the System 45
Enable In-House Air 46
Appendix A: Troubleshooting 47
Powering ON/OFF 47
Loading 48
Running 49
Cleaning 49
Appendix B: Preventive Maintenance,
Decontamination, and Disposal 50
Preventive Maintenance 50
Preventive Maintenance Recommendations 50
Clean the Thermal Cycler Chuck 50
Clean an Interface Plate 51
Clean the Juno System 53
Clean the Touchscreen 55
Replace the Fuses 55
Decontamination and Disposal 56
Biological Agents 56
Hazardous Chemicals 56
Radioactive Materials 56
Juno System: User Guide 5
About This Guide
IMPORTANT Before using the instrument, read and understand the safety guidelines in this
document. Failure to follow these guidelines may result in undesirable effects, injury to
personnel, and/or damage to the instrument or to property.
For complete safety information, see Appendix D.
Purpose
This guide describes how to use the Juno™ system, including the instrument and the system
software. For complete instrument specifications and unpacking/installation instructions, see
the Juno System Site Requirements Guide (PN 100-7072).
Safety Alerts for Chemicals
For hazards associated with chemicals, this document follows the United Nations Globally
Harmonized System of Classification and Labelling of Chemicals (GHS) and uses indicators
that include a pictogram and a signal word that indicates the severity level:
Safety Alerts for Instruments
For hazards associated with instruments, this document uses indicators that include a
pictogram and signal words that indicate the severity level:
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a red
diamond-shaped frame. Refer to the individual safety data sheet (SDS) for the applicable
pictograms and hazards pertaining to the chemicals being used.
DANGER Signal word that indicates more severe hazards.
WARNING Signal word that indicates less severe hazards.
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a black
triangle-shaped frame. Refer to the instrument user guide for the applicable
pictograms and hazards pertaining to instrument usage.
DANGER Signal word that indicates an imminent hazard that will result in severe injury or death
if not avoided.
WARNING Signal word that indicates a potentially hazardous situation that could result in serious
injury or death if not avoided.
CAUTION Signal word that indicates a potentially hazardous situation that could result in minor
or moderate personal injury if not avoided.
IMPORTANT Signal word that indicates information necessary for proper use of products or
successful outcome of experiments.
Juno System: User Guide
About This Guide
Safety Data Sheets
6
Safety Data Sheets
Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals
ordered from Fluidigm, either alone or as part of this system, go to fluidigm.com/sds and
search for the SDS using either the product name or the part number.
Some chemicals referred to in this user guide may not have been provided with your system.
Obtain the SDSs for chemicals provided by other manufacturers from those manufacturers.
Juno System: User Guide 7
Chapter 1: Introducing the Juno System
The Juno System
The Juno™ system is used for genotyping low-concentration DNA with the Juno 96.96
Genotyping IFC (integrated fluidic circuit) and for preparing libraries for targeted sequencing
with the LP 192.24 and LP 48.48 IFCs. This is possible through advanced microfluidics
technology.
In a single workflow, the Juno 96.96 Genotyping IFC integrates preamplification and
genotyping reactions of up to 96 samples and 96 genotyping assays to produce 9,216
genotypes in less than three hours. Samples are loaded into individual inlets of the Juno
96.96 Genotyping IFC, then distributed across multiple reaction chambers in nanoliter-
volume aliquots. With high-quality samples, detecting the specific targets requires a sufficient
number of copies in the final preamplification mix of 4 μL. After genotyping is performed on
the Juno system, the IFC is scanned on the EP1™ system or Biomark™ HD system to collect
genotyping data for later analysis.
The LP 192.24 IFC facilitates parallel amplification of up to 192 samples and up to 2,400
assays for targeted sequencing. The LP 48.48 IFC facilitates parallel amplification of up to 48
samples and up to 4,800 assays for targeted sequencing. Juno performs sample and assay
loading, mixing, thermal cycling, and harvesting automatically for further processing and
targeted sequencing.
NOTE For Research Use Only. Not for use in diagnostic procedures.
Consumables Ordering
To reorder IFCs and reagents, contact your regional Fluidigm sales representative or
distributor. Go to fluidigm.com/contact.html.
Juno System: User Guide
Chapter 1: Introducing the Juno System
Components of the Juno System
8
Components of the Juno System
Figure 1. Front panel of the Juno instrument.
Figure 2. Back panel of the Juno instrument. There are also two USB 2.0 ports on the side of the
instrument.
Touchscreen
interface
Retracted tray
Power and
standby indicator
USB 2.0 ports
Ethernet
port
DVI port
ON/OFF switch
Power socket
Compressed
air input
Exhaust fan
Juno System: User Guide
Chapter 1: Introducing the Juno System
Components of the Juno System
9
Figure 3. Tray and thermal chuck of the Juno instrument. The white notch is used to orient the notched
corner of the IFC in the tray.
Figure 4. Interface Plate Loading Fixture
White notch
Juno System: User Guide
Chapter 1: Introducing the Juno System
Interface Plates and IFCs
10
Figure 5. MX Interface Plate
Interface Plates and IFCs
Table 1 shows the appropriate interface plate for the IFC you are using.
Table 1. Interface plates and supported IFCs
System Software
Ensure that you have installed the Juno system software v3.1 or later if you are using the
Juno 96.96 Genotyping IFC. Install software v3.5 or later if you are using the Juno 96.96
Genotyping IFC or an IFC for the Targeted DNA Sequencing Library application. Install
software v3.11.1 or later if you are using the 24.192 Dynamic Array IFC for Gene Expression.
To get the latest system software, go to fluidigm.com/software.
Interface Plate Name Label Color on Interface Plate Supports…
MX Black 48.48 Dynamic Array™ IFCs
LP 48.48 IFC
qdPCR 37K™ IFC
48.770 Digital Array™ IFC
12.765 Digital Array IFC
SX Silver Juno 96.96 Genotyping IFC
HX Green 96.96 Dynamic Array IFCs
Flex Six™ Genotyping and Gene Expression IFCs
RX Red 192.24 Genotyping and Gene Expression IFCs
24.192 Dynamic Array IFC for Gene Expression
TX Blue LP 192.24 IFC
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