HTA HT2000H Manuale utente

HT2000H_en_n.doc

User Guide

HT2000H, HT2100H, HT2000HT

Static Headspace GC Autosamplers

www.hta-it.com

HT2000H_en_n.doc 16/01/2018

This manual contains information about the product(s) indicated on the cover of this document.

The information contained in this document is subject to change without notice.

This document is for information only, the manufacturer accepts no liability for errors contained

herein or for incidental or consequential damage with the furnishing, performance, or use of this

material.

Unless otherwise specified, each reference to names or belongings is purely casual and has the

sole purpose of illustrating the product described here. Contents of this publication may not be

reproduced in any form or by any means (including electronic storage and retrieval or translation

into a foreign language), without prior agreement and written consent from the copyright owner.

All rights are reserved.

Manufacturer: HTA s.r.l. - 77-79, via del Mella, I-25131 Brescia (BS), Italy - Tel: +39 030 3582920 -

E-mail: info@hta-it.com

HT2000H_en_n.doc Page 3 of 167

Table of Contents

INTRODUCTION .......................................................................................................................8

1.1

NTENDED USE AND RESTRICTIONS

..........................................................................................8

1.2

YMBOL TABLE

.....................................................................................................................9

1.3

ARNING

...........................................................................................................................10

1.4

TANDARDS

OMPLIANCE

...................................................................................................13

1.5

ENERAL SPECIFICATION

.....................................................................................................14

1.6

UNCTIONS AVAILABLE ONLY THROUGH

HTA

UTOSAMPLER

ANAGER

....................................14

1.7

ERSIONS

..........................................................................................................................15

1.7.1

Description...................................................................................................................................... 15

1.7.2

Options............................................................................................................................................ 15

1.8

ECHNICAL

PECIFICATIONS

................................................................................................16

1.9

SOFTWARE REQUIREMENTS

(HTA

UTOSAMPLER

ANAGER

).............................................19

EQUIPMENT DESCRIPTION...................................................................................................20

2.1

ARTS

EFINITION

..............................................................................................................20

2.1.1

HT2000H/HT2000HT Overview .....................................................................................................20

2.1.2

HT2100H Overview ........................................................................................................................ 25

2.1.3

Syringe location .............................................................................................................................. 28

2.1.4

Connection panel ............................................................................................................................ 30

2.2

OVING PARTS

...................................................................................................................32

2.3

OOL KIT

............................................................................................................................33

2.4

OUCH SCREEN DISPLAY DESCRIPTION

(HT2000H/HT2000HT) ..............................................34

2.4.1

Touchscreen use.............................................................................................................................. 34

2.4.2

Screen templates ............................................................................................................................. 35

2.4.3

Limited Warranty ............................................................................................................................ 42

INSTALLATION .......................................................................................................................43

3.1

NPACKING AND PRELIMINARY OPERATIONS

..........................................................................43

3.2

UTOSAMPLER ASSEMBLY

...................................................................................................44

3.2.1

How to lift and carry the autosampler ............................................................................................ 47

3.3

NSTALLATION

.....................................................................................................................48

3.3.1

Autosampler installation on the mounting plate ............................................................................. 48

3.3.2

Safety Lock setting .......................................................................................................................... 53

3.3.3

Electrical connections..................................................................................................................... 54

3.3.4

Purge line connections.................................................................................................................... 55

3.4

TART

-U

..........................................................................................................................56

HT2000H_en_n.doc Page 4 of 167

3.5

CONNECTION AND SOFTWARE INSTALLATION

.....................................................................56

3.5.1

Autosampler controlled by LAN-connected PC ..............................................................................57

3.5.2

Autosampler connected directly to the PC ...................................................................................... 65

3.6

CCESSORIES INSTALLATION

................................................................................................66

3.6.1

External pressure regulator installation ......................................................................................... 67

3.6.2

Swagelok Adapter installation ........................................................................................................69

3.6.3

System integrity tool installation.....................................................................................................70

3.7

REPARING SAMPLE VIAL

.....................................................................................................72

3.7.1

Vial capping (Crimping Cap).......................................................................................................... 73

3.8

INAL INSTALLATION OPERATIONS

.........................................................................................74

3.9

ASIC TRAINING

..................................................................................................................74

SET UP OPERATIONS............................................................................................................76

4.1

IEW

ARAMETERS

.............................................................................................................76

4.2

ETUP

...............................................................................................................................77

4.2.1

Setup: Alignment.............................................................................................................................77

4.2.2

Setup: Syringe warmer assembly installation .................................................................................80

4.2.3

Setup: Manual operations............................................................................................................... 89

4.2.4

Setup: Configuration.......................................................................................................................92

4.2.5

Setup: Service.................................................................................................................................. 98

PROGRAMMING .....................................................................................................................99

5.1

HT2100H:

ETHODS AND

EQUENCE

..................................................................................99

5.2

HT2000H/HT2000HT:

ETHODS

........................................................................................99

5.2.1

Method Menu .................................................................................................................................. 99

5.2.2

Method X: Setting method parameters............................................................................................99

5.2.3

Method tools.................................................................................................................................. 103

5.2.4

Example ........................................................................................................................................ 103

5.3

HT2000H/HT2000HT:

EQUENCE

....................................................................................105

5.3.1

Sequence Menu ............................................................................................................................. 105

5.3.2

Setting sequence step parameters ................................................................................................. 105

5.3.3

Sequence tools............................................................................................................................... 106

HT2100H OPERATIONS .......................................................................................................107

6.1

EYS

...............................................................................................................................107

6.1.1

Keys Settings ................................................................................................................................. 108

6.2

TATUS

NDICATORS

.........................................................................................................108

6.2.1

Reference table.............................................................................................................................. 108

HT2000H_en_n.doc Page 5 of 167

HT2000H/HT2000HT OPERATIONS .....................................................................................111

7.1

OME SCREEN

..................................................................................................................111

7.1.1

Home screen: Standard User Interface......................................................................................... 111

7.1.2

Home screen: Quick Start User Interface ..................................................................................... 112

7.2

INGLE INJECTION

.............................................................................................................112

7.3

UTOMATIC RUN

...............................................................................................................113

7.3.1

Interrupting an automatic run....................................................................................................... 114

7.3.2

Immediate Injection....................................................................................................................... 114

7.3.3

Skip Next Vial option..................................................................................................................... 114

7.4

YRINGE PURGE

...............................................................................................................115

7.5

OAD AND

NLOAD

AMPLE

IAL

.......................................................................................115

7.6

ETTINGS

ENU

...............................................................................................................115

7.6.1

Change tray/rack type................................................................................................................... 116

7.6.2

Eco Savings ................................................................................................................................... 117

TROUBLESHOOTING ...........................................................................................................119

8.1

NTRODUCTION

.................................................................................................................119

8.2

TART UP ERRORS

............................................................................................................120

8.2.1

Safety lock error............................................................................................................................ 120

8.2.1

Vial locator error ...................................................................................................................... 120

8.2.2

Memory error................................................................................................................................ 120

8.3

ETHOD ERRORS

.............................................................................................................121

8.3.1

Fill Volume/Pull Up Strokes settings incompatibility ................................................................... 121

8.3.1

Fill volume/Syringe Pre-Fill settings incompatibility ............................................................... 121

8.4

OUCH

ENSOR PROBLEMS

...............................................................................................122

8.5

ALLING VIALS

..................................................................................................................122

8.6

BSTACLE FOUND

.............................................................................................................123

8.7

BSTACLE FOUND DURING COVER MOVEMENT

.....................................................................123

8.8

ISMATCH ERRORS

...........................................................................................................124

8.8.1

Single injection mismatch error .................................................................................................... 124

8.8.2

Automatic run mismatch errors .................................................................................................... 124

8.9

ISSING ERRORS

..............................................................................................................126

8.9.1

Missing: front injector alignment .................................................................................................. 126

8.9.2

Missing: rear injector alignment .................................................................................................. 126

8.9.3

Missing: touch & plunger zero...................................................................................................... 126

8.10

IMEOUT ERROR

...............................................................................................................127

8.10.1

Peripheral communication timeout ........................................................................................... 127

HT2000H_en_n.doc Page 6 of 167

8.11

ECHANICAL ERRORS

.......................................................................................................127

8.12

EATING ERRORS

.............................................................................................................128

8.13

VEN ERROR

...................................................................................................................128

8.14

YSTEM INTEGRITY TEST FAIL

.............................................................................................128

8.15

AMAGE TO SYRINGE NEEDLE

............................................................................................129

8.15.1

Syringe inspection ..................................................................................................................... 130

8.16

RAY EMERGENCY RELEASE

...............................................................................................131

8.16.1

Tray opening by hand ............................................................................................................... 131

8.17

“HTA

UTOSAMPLER

ANAGER

”

TROUBLESHOOTING

............................................................133

8.17.1

Autosampler and PC do not communicate (connection by LAN)............................................. 133

8.18

NALYTICAL TROUBLESHOOTING

.........................................................................................135

8.18.1

Reproducibility issue................................................................................................................. 135

8.18.2

Peak distortion or tailing .......................................................................................................... 136

8.18.3

Carry over issue........................................................................................................................ 136

8.18.4

Unexplained chromatographic peaks ....................................................................................... 136

8.18.5

Retention times not repeatable.................................................................................................. 136

8.18.6

Faulty temperature read-out..................................................................................................... 137

MAINTENANCE.....................................................................................................................138

9.1

RDINARY MAINTENANCE

..................................................................................................138

9.1.1

Cleaning of vial rack/tray ............................................................................................................. 138

9.1.2

External cleaning of cabinet ......................................................................................................... 139

9.1.3

Incubation oven cleaning .............................................................................................................. 139

9.1.4

Touch screen display cleaning ...................................................................................................... 140

9.1.5

Syringe replacement in the syringe warmer assembly .................................................................. 140

9.1.6

Septum of the System integrity tool replacement....................................................................... 150

9.1.7

Preventive maintenance pack.................................................................................................... 151

9.2

XTRAORDINARY MAINTENANCE

.........................................................................................151

UNINSTALLING AND DISPOSAL.......................................................................................152

10.1

UTOSAMPLER UNINSTALLING

............................................................................................152

10.2

UTOSAMPLER SHIPPING

...................................................................................................153

10.2

UTOSAMPLER DISPOSAL

...................................................................................................156

10.3

ISPOSAL INSTRUCTIONS

...................................................................................................156

APPENDIX A – GLOSSARY ..............................................................................................158

APPENDIX B – CONSUMABLES .......................................................................................160

12.1

YRINGES

........................................................................................................................160

HT2000H_en_n.doc Page 7 of 167

12.1.1

How to select the correct syringe type (only for HT2000HT)................................................... 160

12.2

AMPLE VIALS

..................................................................................................................161

12.2.1

Vial specifications..................................................................................................................... 161

12.2.2

Cap specifications..................................................................................................................... 164

12.2.3

Septa specifications................................................................................................................... 164

APPENDIX C – CONNECTION PANEL..............................................................................165

13.1

CONNECTOR

...............................................................................................................165

13.1.1

Typical GC interface................................................................................................................. 166

13.2

RS232C

INTERFACE

(

OPTIONAL

)........................................................................................166

HT2000H_en_n.doc Page 8 of 167

1 Introduction

1.1 Intended use and restrictions

HT2000H, HT2100H and HT2000HT are static headspace autosamplers for gas

chromatography.

Headspace gas chromatography (GC) is a technique used for the concentration and analysis of

volatile organic compounds in almost any sample matrix.

The autosampler must be used in a professional environment, and only by properly trained

users. The autosampler must be used according to the regulations in force regarding the safety

in testing laboratories.

A list (not exhaustive) of typical applications of the autosampler is reported below:

•Environmental analysis

•Quality control in the chemical and pharmaceutical industry

•Studies related to pharmaceuticals

•Drug discovery

•Other techniques or areas of application are possible.

The safety measures and precautions regarding use, maintenance and repair, are not intended

for applications that require the use of toxic or infectious substances.

Every possible mixture or omission of a component cannot be recognized or previously

prevented. The operator that will use the autosampler must be trained about the used

substances, their hazards, their use and their proper disposal. For the correct use of the used

substances, refer to chemicals safety data sheets (MSDS) and to the regulations, laws,

directives and safety decrees currently in force that regulate the behaviour inside the testing

laboratories.

The insertion and positioning of the sample (vial), the insertion of syringes and all the other

preparation activities are under the sole responsibility of the user. The user must operate in

accordance with the law, in particular the ones concerning the safety of testing laboratories and

accident prevention.

The operator must be trained to operate the equipment in accordance with the safety standards

for the laboratories. The autosampler does not have specific protection mechanisms against

substances that are corrosive, potentially infectious, toxic and radioactive or against other

substances that can be hazardous to health. However, when the autosampler is used, because

HT2000H_en_n.doc Page 9 of 167

of the handled substances, the user could need to use individual protection measures such as

gloves, masks and goggles; the use of such devices must comply with the regulations currently

in force regarding the safety of testing laboratories.

The use of the autosampler for any technique invasive for humans or animals is explicitly

prohibited. The autosampler must be used only in the manner described in the usage

instructions. Any other use may affect the autosampler and operator safety.

HTA s.r.l. Is not responsible for damage caused, even only in part, by improper use of the

autosampler, by unauthorized modification of it, by different or missing execution of the

procedures described in this manual, or by the use of the autosampler violating the applicable

laws and regulations.

The autosampler has been designed and tested in accordance with current safety standards.

1.2 Symbol table

Please carefully read this table before using your autosampler. These symbols are used in this

manual.

Generic warning

Attention: dangerous voltage

Attention: mechanical hazard

Biological risks (if applicable for the substances used with the autosampler)

Hot surface

HT2000H_en_n.doc Page 10 of 167

1.3 Warning

Read the following warnings before unpacking or using the unit.

See paragraph 1.1 “Intended use and restrictions” and be sure you have understood

it.

See EU Declaration of Compliance enclosed with the autosampler.

Before removing the autosampler from the box, please carefully read paragraphs 3.1

“Unpacking and preliminary operations” and 3.2 “Autosampler assembly”.

Before switching the autosampler ON check the correct voltage.

Electrical Hazards. Every analytical instrument has specific hazards, so ensure you

read and comply with the following precautions.

Use only the power supply system supplied with the autosampler. The use of a

different system could cause damage to the equipment and/or compromise safety.

There are no operator-serviceable or replaceable parts inside the power supply. In

case of failure, contact the Technical Assistance Centre. Violations of these directives

imply the immediate invalidation of the warranty and service contract.

The supplied power cord must be inserted into a power outlet with a protective earth

contact (ground). When using an extension cord, make sure that the cord also has an

earth contact.

Do not change the external or internal grounding connections. Tampering with or

disconnecting these connections could endanger you and/or damage the

autosampler.

The product is designed and tested in accordance with international safety standards.

You do not need to make any changes to the electrical connections or to the chassis

of the autosampler in order to ensure safe operation.

Do not turn the autosampler on if you suspect that it has incurred any kind of electrical

damage or if it shows visible signs of damage. If, for any reason, the safety

protections were compromised or the autosampler shows visible signs of damage,

disconnect the power cord and contact an HTA representative. The power cord

connector is considered to be the disconnecting device of the product. Electrical

damage may have occurred if the autosampler shows visible signs of damage, or has

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