Ibidi Universal Fit 10918 Manuale utente

10918 ibidi Heating System, Universal Fit for 1 Chamber

Version 2.2

Instruction Manual

ibidi Heating System, Universal Fit

Lid

I 42.1

S[ 42.0] Run

Plate Glass

37.0

37.0 N.C.

37.0

Setup

N.C.

ibidi Heating System, Universal Fit

Version 2.2 (May 23, 2019) 2 Instruction Manual

ibidi Heating System, Universal Fit

Contact

ibidi GmbH

Lochhamer Schlag 11

82166 Gr¨

afelﬁng

Germany

Phone: +49 89 / 520 46 17 - 0

Fax: +49 89 / 520 46 17 - 59

E-mail: [email protected]

Internet: ibidi.com

Instruction Manual 3 Version 2.2 (May 23, 2019)

ibidi Heating System, Universal Fit

Contents

1 Preamble 6

1.1 Introduction ........................................... 6

1.2 Safety Symbols ......................................... 6

1.3 Nomenclature .......................................... 7

1.4 Speciﬁcations .......................................... 7

1.5 Disclaimer ............................................ 10

1.6 Safety Considerations ...................................... 10

1.7 Regulatory Statement ...................................... 11

1.8 Limited Warranty ........................................ 12

1.9 Transporting the ibidi Heating System, Universal Fit .................... 12

1.10 Repairing the ibidi Heating System, Universal Fit ..................... 13

1.11 Waste Disposal – WEEE/RoHS Compliance Statement .................. 13

1.11.1 EU Directive WEEE ................................... 13

1.11.2 EU Directive RoHS ................................... 13

2 Intended Use 14

3 Principle 14

4 Equipment 15

4.1 Components of the ibidi Heating System, Universal Fit .................. 15

4.2 Combination Options ...................................... 16

4.3 Temperature Controller ..................................... 17

4.4 Heated Lid ............................................ 18

4.5 Heated Plate ........................................... 19

4.6 Heating Inserts ......................................... 20

4.6.1 Heating Insert µ–Slide ................................. 20

4.6.2 Heating Insert µ–Dish 35mm, high and Heating Insert µ–Dish 35mm, low ...... 20

Version 2.2 (May 23, 2019) 4 Instruction Manual

ibidi Heating System, Universal Fit

5 Operation 22

5.1 Installation and Connection of the Parts ........................... 22

5.2 Start the ibidi Heating System, Universal Fit ........................ 23

5.3 Setting Parameters in the Front Display ........................... 23

5.3.1 Run/Stop Mode .................................... 24

5.3.2 Incubation Parameters ................................. 24

5.3.3 Setup Menu ....................................... 24

5.4 Temperature Adjustment in the Sample ........................... 26

5.5 Sample Preparation ....................................... 27

6 IncuControl Software 28

7 Maintenance 29

7.1 Disinfection and Cleaning ................................... 29

7.2 Inﬂuence of Ambient Temperature and Ventilation ..................... 29

8 Troubleshooting 30

8.1 Focus not Stable ......................................... 30

8.2 Evaporation Is too High .................................... 30

8.3 Condensation Inside the Stage Top Incubator ........................ 30

Instruction Manual 5 Version 2.2 (May 23, 2019)

ibidi Heating System, Universal Fit

1 Preamble

1.1 Introduction

This manual is your guide to using the ibidi Heating System, Universal Fit for cell culture experi-

ments on an optical microscope. It instructs ﬁrst-time users how to use the instrument, and serves as

a reference for experienced users.

Before using the ibidi Heating System, Universal Fit, please read this instruction manual carefully

and make sure that the contents are fully understood. This manual should be easily accessible to the

operator at all times during instrument operation. If this manual gets lost, order a replacement from

www.ibidi.com.

To ensure safe operation, the ibidi Heating System, Universal Fit must only be operated with the

supplied components and according to the instruction manual.

1.2 Safety Symbols

Note that the signal words WARNING,CAUTION and NOTE have speciﬁc meanings in this man-

ual. Do not proceed beyond a signal word until you have performed the indicated actions.

WARNING! A potentially hazardous situation which, if not avoided, could result in se-

rious injury or even death. Warning messages in the text are displayed in a

gray shaded box.

CAUTION A potentially hazardous situation which, if not avoided, could result in minor

or moderate injury. It is also used to alert against damaging the equipment

or the instrument.

NOTE Additional information to help achieve optimal instrument and assay perfor-

mance.

Symbols on the product identiﬁcation label and back panel of the device:

CE Marking: This symbol indicates the product’s compliance with EU legis-

lation.

This label is positioned on the back of the device and prompts you to read the

manual before using the device.

Product disposal: The symbol indicates that this product must be recy-

cled/disposed of separately from other household waste. See page 13 for

details.

Version 2.2 (May 23, 2019) 6 Instruction Manual

ibidi Heating System, Universal Fit

1.3 Nomenclature

Temperature Controller Heated Lid Heated Plate

1.4 Speciﬁcations

Only operate the Heating System in the range of the speciﬁcations given below:

Table 1 – Speciﬁcations of the ibidi Heating System, Universal Fit

Electrical Speciﬁcations Power Supply

Protection class I

International protection marking

(IEC 60529)

IP 20

Overvoltage category II

External power supply AC 100-240 V, 50/60 Hz, 2 A

Input line voltage Temperature

Controller

DC 24 V, 6.67 A, 160 W

Output voltage to channel 1

(Heated Lid), Universal Fit

DC 10 V, max. 5 A

Output voltage to channel 2

(Heated Plate), Universal Fit, for 1

Chamber

DC 12 V, max. 5 A

Operating and Storage Conditions

Operating site Indoor use only

Operating temperature 18-30°C/64-86°F (min 5°C/9°F less than set temperature)

Operating humidity max. 80% relative humidity (RH)

Operating altitude max. 2000 m (atmospheric pressure 800-1060 hPa/11.6-15.4

psi)

Storage conditions -5-50°C/23-122°F, humidity <60% relative humidity (RH)

Outer Dimensions and Characteristics of the Components

Temperature Controller 90 mm × 170 mm × 230 mm

Weight: 1720 g/3.8 lbs

Heated Lid 19 mm × 85.5 mm × 127.5 mm (134.5 mm with cover ridge)

Length of cable: 1.5 m

Instruction Manual 7 Version 2.2 (May 23, 2019)

ibidi Heating System, Universal Fit

Table 1 – (continued)

Connector to Gas Incubation: Female Luer Lock

Heated Plate 12 mm × 85.5 mm × 127.5 mm

Observation area: 40 mm × 82 mm

Length of cable: 1.5 m

Heated Plate assembled with

Heated Lid

Height: 25.5 mm

Weight: 330 g/0.46 lbs

Heating Inserts all 47 mm × 97 mm

Heating Insert 35 mm Dish high Height without blank holder: 8 mm

Height with blank holder: 16-18 mm

Observation area: 22 mm

Weight insert: 50 g/0.11 lbs

Weight blank holder: 72 g/0.16 lbs

Heating Insert 35 mm Dish low Height without blank holder: 8 mm

Height with blank holder: 13-15 mm

Observation area: 22 mm

Weight insert: 50 g/0.11 lbs

Weight blank holder: 47 g/0.11 lbs

Heating Insert Slides Height without blank holder: 8 mm

Height with blank holder: 13-15 mm

Observation area: 49 mm × 22 mm

Weight insert: 42.5 g/0.94 lbs

Weight blank holder: 38.5 g/0.85 lbs

USB cable Length: 1.8 m

Power supply cable Length: 2.0 m (power supply to wall)

Length: 1.2 m (power supply to device)

Temperature Control Range

Heated Lid Ambient temperature (min. 18°C) to +45°C

Accuracy: ±0.2°C (at sensor location)

±5°C (entire heated glass)

Heated Plate Ambient temperature (min. 18°C) to +45°C

Accuracy: ±0.2°C (at sensor location)

±0.5°C (entire heated plate)

Recommended Temperatures before Adjustment

See Section 5.4

USB Interface

Connector type USB 2.0 Connector Type B

Recommended USB cable Tripp Lite UR022-006 (shielded)

Driver FTDI VCP driver

Version 2.2 (May 23, 2019) 8 Instruction Manual

ibidi Heating System, Universal Fit

Table 1 – (continued)

Microscope Requirements

Working distance condenser ≥26 mm

Stage holder Holder for standard multi-well plates

Optical Properties Glass Lid

Glass Selected HQ Floatglass

Thickness of the glass plate 1.1 mm

Refractive index glass nD= 1.520 (588 nm)

ITO coating Thickness: 100 nm

Passivation layer Thickness: 20-25 nm

Refractive index ITO with passiva-

tion layer

1.95

Instruction Manual 9 Version 2.2 (May 23, 2019)

ibidi Heating System, Universal Fit

1.5 Disclaimer

• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of

product use.

• The contents of this manual are subject to change without notice for product improvement.

• This manual is considered complete and accurate at publication.

• This manual does not guarantee the validity of any patent rights or other rights.

• If an ibidi software program doesn’t function properly, this may be caused by a conﬂict from

another program operating on the computer. In this case, take corrective action by uninstalling

the conﬂicting product(s).

• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.

1.6 Safety Considerations

WARNING!

• Only operate the ibidi Heating System, Universal Fit with the supplied components.

• Only use the cables and plugs delivered with the system. The power plug of the control unit

must be inserted in an outlet with a ground (earth) contact.

• Do not replace detachable power cables by power cables with inadequate speciﬁcations. By

violating these instructions you risk electric shock and ﬁre.

• Only use extension cables that have a protective ground wire.

• Do not operate the ibidi Heating System, Universal Fit under conditions that pose a risk of

explosion, implosion, or the release of gases. Only operate the ibidi Heating System, Universal

Fit with aqueous solutions.

• Do not operate a damaged ibidi Heating System, Universal Fit. If the housing seems damaged

or something is rattling inside the controller, contact the ibidi service hotline for repair.

• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.

Avoid touching the temperature-controlled parts of the system when you have set the Temper-

ature Controller to high temperatures.

CAUTION

• Ensure that the external power supply is easily accessible. The ibidi Heating System, Universal

Fit must be installed in a manner such that none of its components hinders access to the external

power supply.

• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage

to the instrument.

Version 2.2 (May 23, 2019) 10 Instruction Manual

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