GravityPLUS™ Hanging Drop System Manual
8
Generating 3D microtissues
Generating 3D microtissues is a straightforward process that works with the vast
majority of cell types capable of forming tissues in vivo. In addition to the pro-
cess overview in this chapter, Annex 6 illustrates the formation of NIH/3T3 mouse
broblast microtissues in detail as an example for your own process.
Additional materials required
1. Mammalian cells, either cell lines or primary cells
2. 3D InSight™ Tumor Microtissue Media Kit (InSphero, cat. no. CS-17-001-01 -
includes 3D InSight™ Tumor Re-aggregation Medium (CS-07-111-02) and
3D InSight™ Tumor Maintenance Medium (CS-07-112-01)
3. Inverted microscope with a 5× objective or a 10× long distance objective with
a distance ring (see also Appendix 1)
4. Cell counter, e.g. Neubauer chamber
5. 8- or 12-channel pipette (e.g. Viao 10.0-300.0 μl, Integra Biosciences,
InSphero, cat. no. IS-001-01)
6. Medium reservoir for multichannel pipettes
7. For microscopic observation of the full GravityPLUS™ Plate, an additional
GravityPLUS™ bottom plate is recommended
8. Microplate centrifuge
9. Humidied CO2incubator
