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LeicaM50
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User Manual
Leica M-series User Manual 2
General Instructions
Safety concept
Before using your microscope for the rst time,
please read the "Safety concept" brochure
included with your instrument. It contains addi-
tional information about handling and care.
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
★Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
★Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This may damage surfaces and
abraded particles may contaminate prepa-
rations.
★In most cases, we can provide special solu-
tions on request. Some products can be
modied, and we can oer other accesso-
ries for use in clean rooms.
Servicing
★Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
★Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.
Leica M-series User Manual 3
Important Safety Notes
User Manual
The individual modules of the Leica M stereo-
microscopy series include an interactive
CD-ROM with all relevant user manuals in
20other languages. Keep it in a safe place, and
readily accessible to the user. User manuals and
updates are also available for you to download
and print from our web site at www.stereomi-
croscopy.com.
This operating manual describes the special
functions of the individual modules of the Leica
M stereomicroscopy series and contains impor-
tant instructions for their operational safety,
maintenance, and accessories.
The "Safety concept" booklet contains additional
safety information regarding the service work,
requirements and the handling of the stereomi-
croscope, accessories and electrical accessories
as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instru-
ments, read the user manuals listed above. In
particular, please observe all safety regulations.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica M-series User Manual 4
Symbols Used
Warning of a danger
•This symbol indicates especially impor-
tant information that must be read and
complied with.
Failure to comply can cause the following:
★Hazards to personnel
★Functional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
★Hazards to personnel
★Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional informa-
tion or explanations that are intended to
provide clarity.
Explanatory notes
★This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the gures and the
items within those gures.
Leica M-series User Manual 5
Safety Regulations
Description
★The individual modules fulll the highest
requirements for observation and docu-
mentation of Leica stereomicroscopes of
the M series.
Designated use
★Refer to "Safety Concept" booklet
Non-intended use
★Refer to "Safety Concept" booklet
Never use M series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specically intended for that
purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica aliate must be consulted whenever
the instrument is altered, modied or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Place of use
★Refer to "Safety Concept" booklet
★Set up the electrical components at least
10cm from walls and combustible objects.
★Avoid large temperature uctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
★In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the buildup of
fungus.
Responsibilities of person in charge of
instrument
★Refer to "Safety Concept" booklet
Ensure that:
★The M series stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
★All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Leica M-series User Manual 6
Safety Regulations (cont'd.)
Repairs, service work
★Refer to "Safety Concept" booklet
★Only original Leica Microsystems spare
parts may be used.
★Before opening the instruments, switch o
the power and unplug the power cable.
★Touching the live circuit can cause injury.
Transport
★Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
★In order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Installation in third-party products
★Refer to "Safety Concept" booklet
Disposal
★Refer to "Safety Concept" booklet
Legal requirements
★Refer to "Safety Concept" booklet
EC Declaration of Conformity
★Refer to "Safety Concept" booklet
Health risks
Workplaces equipped with stereomicroscopes
facilitate and improve the viewing task, but
they also impose high demands on the eyes
and holding muscles of the user. Depending
on the duration of uninterrupted work, asthe-
nopia and musculoskeletal problems may
occur. For this reason, appropriate measures
for reduction of the workload must be taken:
optimal arrangement of workplace, work
assignments and work ow (changing tasks
frequently), thorough training of the personnel,
giving consideration to ergonomic and organi-
zational aspects.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infec-
tions of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Leica M-series User Manual 7
Safety Regulations (cont'd.)
Light sources: safety regulations
Protective measures of the manufacturer:
★UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
★Dummy lter carriers in the free positions
of the rapid lter changer prevent direct UV
radiation from reaching the eyes.
★UV lters are installed in the observation
beam paths to protect the eyes.
★The stray-light protection on the lamp
housing prevents irradiation of the hands.
Warning
★UV radiation could damage the eyes.
Therefore:
★Never look into the light spot on the speci-
men plane without a UV protection screen.
★Never look into the eyepieces if no excita-
tion lter is in the beam path.
★Fill empty lter positions with dummy lter
carriers (e.g. M165 FC).
★Do not select a white, strongly reective
background for the specimen.
Supply unit
Always unplug the supply unit from the power
supply:
★When installing and disassembling the
lamp housing
★Before opening the lamp housing
★When replacing the high-pressure mercury
lamp and other parts, such as the heat-
absorbing lter or the collector
★During maintenance work on the supply
unit
Leica M-series User Manual 8
Safety Regulations (cont'd.)
Lamp housing
★Never open the lamp housing while the
lamp is switched on. Risk of explosion, UV
exposure, blinding!
★Before opening the lamp housing, allow it
to cool o for at least 15 minutes. Danger
of explosion!
★Never cover the air duct on the lamp hous-
ing. Danger of re!
Mercury lamp
★Follow the user manual and safety regula-
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
★For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
★When it has reached the end of its rated life
(follow the manufacturer's specications
and the minute meter on the supply unit).
★To minimize the risk of explosion, replace a
discolored mercury lamp promptly.
★Leica Microsystems assumes no liability
for damage caused by exploding, incor-
rectly installed or improperly used mercury
lamps.
Inhaltsverzeichnis
General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Regulations 5
Safety Regulations (cont'd.) 6
Safety Regulations (cont'd.) 7
Safety Regulations (cont'd.) 8
The Leica
M series
Congratulations! 12
The Modular Design: Everything is Relative 13
Maximum Compatibility 14
On We Go 15
Assembly
Base and Focusing Column 17
Microscope Carrier 18
Optics Carrier 19
Tube 20
Eyepieces 21
Objective 22
Leica LED5000 CXI (Coax Illumination) 23
Leica LED5000 CXI (cont'd.) 24
Leica LED3000 NVI™ (Near Vertical Illumination) 25
Leica LED3000 NVI™ (cont'd.) 26
Quick Start Guide
The Reliable Route to Success 28
Overview of an M Series Microscope 29
The Correct Interpupillary Distance 30
Using the Eyepieces 31
Focusing 32
Adjusting the Resistance of the Focus Drive 33
Changing the Magnication (Zoom) 34
Ratchet Steps and Magnication Levels 35
Eyepieces & Tubes
Magnication Factor of the Eyepieces 37
Health Notes 38
Dioptric Correction 39
Dioptric Correction and Parfocality 40
Graticules 41
ErgoTube 10° to 50° 42
Photography & Video
Photography & Video 44
Leica M-series User Manual 9
Table of Contents
Photo Tubes and C-mounts 45
Video/Phototubes HD F & HD V 46
Objectives and
Optical Accessories
The Dierent Types of Objectives 48
System Illumination
Leica LED3000 NVI™ (Near Vertical Illumination) 50
Leica LED5000 CXI (Coax Illumination) 51
Dimensional Drawings
LeicaM50 53
LeicaM50 54
LeicaM50 55
Leica M60 56
Leica M80 57
Leica M80 58
Leica M80 59
Leica M80 60
Leica M80 61
Appendix
Calculating the Total Magnication and Field of View Diameter 63
Care, Maintenance, Contact Persons 64
Care, Maintenance, Contact Persons (cont'd.) 65
Leica M-series User Manual 10
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