LI-COR Pearl Trilogy Manuale utente

Pearl® Trilogy

Accessories Manual

CE Marking:

This product (model number 5700-DS) is a CE-marked product. For conformity information, contact LI-COR

Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor.

Notes on Safety

LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety

of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The

Pearl Docking Station and Pearl Clean Box were intended to be used by qualified personnel. Read this entire manual

before using the Pearl Docking Station or Pearl Clean Box.

Equipment Markings:

The product is marked with this symbol when it is necessary for you to refer to the

manual or accompanying documents in order to protect against damage to the product.

The product is marked with this symbol when a hazardous voltage may be present.

Manual Markings:

WARNING Warnings must be followed carefully to avoid bodily injury.

CAUTION Cautions must be observed to avoid damaging your equipment.

NOTE Notes contain additional information and useful tips.

IMPORTANT Information of importance to prevent procedural mistakes in the operation of the

equipment or related software. Failure to comply may result in a poor experimental

outcome but will not cause bodily injury or equipment damage.

Federal Communications Commission Radio Frequency Interference Statement

WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in

accordance with the instruction manual, may cause interference to radio communications. It has been tested and

found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules,

which are designed to provide a reasonable protection against such interference when operated in a commercial

environment. Operation of this equipment in a residential area is likely to cause interference in which case the

user, at his own expense, will be required to take whatever measures may be required to correct the interference.

iii

Notice

The information contained in this document is subject to change without notice. LI-COR MAKES NO

WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO

THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.

LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with

the furnishing, performance, or use of this material.

This document contains proprietary information that is protected by copyright. All rights are reserved. No part of

this document may be photocopied, reproduced, or translated to another language without prior written consent of

LI-COR, Inc.

Printing History

Publication Number 984-18616

Printed:February 2015

Revised:September 2019

LI-COR, Pearl, In-Cell Western, IRDye, Odyssey, and Image Studio are registered trademarks of LI-COR, Inc. in the

United States and other countries. All other trademarks belong to their respective owners. The Odyssey Infrared

Imaging Systems and IRDye reagents are covered by U.S. patents, foreign equivalents, and patents pending.

Chapter 1: Pearl Docking Station

Description 1

Assembling the Pearl Docking Station 2

Docking Station Operation 6

Routine Maintenance 8

Docking Station Specifications 8

Chapter 2: Pearl Clean Box

Description 9

Installation 9

Connecting a HEPA Filter to the Docking Station 9

Connecting a HEPA Filter to the Pearl® Imager 10

Nose Cone Installation and Replacement 10

Sanitizing the Pearl Clean Box 11

Clean Box Operation 11

Maintenance of the Clean Box Window 12

Scratches 12

Smudging 12

Chemicals 13

Cleaning Procedure 13

Replacing Heater Plate Contact Pins 13

Table of Contents

Chapter 1: Pearl Docking Station

Description

Figure 1-1. Pearl Docking Station (left) with optional

Pearl Clean Box (mounted) and isoflurane vaporizer

(right) of the SmartFlow Anesthesia System.

The Pearl Docking Station is an optional,

external accessory that duplicates many of the

features of the Pearl®Imager imaging drawer.

The main features and functions of the Docking

Station are listed below.

lDuplicates the docking hardware in the

Pearl Imager imaging drawer, allowing an

accessory imaging bed to be locked in place

on the docking station similar to the Pearl

imaging drawer.

lProvides temperature and anesthesia gas con-

trol to a Pearl accessory imaging bed outside

of the Pearl Imager, allowing animals to be

prepared for imaging at an external station.

lWarms the heater plate in any of the Pearl

accessory imaging beds (when docked) to 38

°C so animals can be placed on warm ima-

ging beds before transfer to the Pearl

Imager.

lProvides a rotameter, isoflurane scrubber, and anesthesia gas flow system similar to the Pearl

Imager when an accessory imaging bed is locked in place on the docking station.

This chapter provides information on assembly and operation of the Pearl Docking Station only.

The remaining chapters provide specific operational details on each of the accessory imaging beds.

Information on the standard Pearl Imaging Bed is not provided in this manual since it can be found

in the Pearl Imager Operator's Manual.

Page 1

Chapter 1: Pearl Docking Station

Assembling the Pearl Docking Station

WARNING: Isoflurane gas can leak in the amount supplied to the Pearl Imaging System

Docking Station if the product is not used properly. Carefully follow the instructions in this

chapter to ensure that the scavenging charcoal filter and user-supplied anesthesia system are

properly connected, and that the nose cone on the imaging bed has a nose cone plug inserted

when not in use.

AVERTISSEMENT:

L'anesthésie d'isoflurane peut fuir dans la quantité fournie à la station d'amarrage de Pearl si

le produit n'est pas employé correctement. Suivez soigneusement les instructions dans ce

chapitre pour vous assurer que le port d'échappement est relié à un filtre à charbon, que le

système d'anesthésie fourni par l'utilisateur est correctement relié, et que le cone de nez est

équipé d'un bouchon inséré.

1) Unpack the Pearl®Imaging System Docking Station and any other accessory imaging beds, such

as the Pearl Clean Box, so all parts are available for assembly.

2) Loosen the thumb nuts on the back of the rotameter stand. Orient the rotameter with tubing to

the back of the stand as shown below. Connect the rotameter to the rotameter stand by sliding

the rotameter over the mounting studs (similar to the Pearl Imaging System instrument) and

tightening the thumb nuts.

Figure 1-2. Docking station rotameter connects to the stand via two

thumb nuts with the rotameter oriented to the front of the stand.

Page 2

Pearl® Imager Accessories

3) Connect the tube from the port labeled “OUT” on the rotameter to the port labeled “IN” on the

back of the docking station. The gender of the connector prevents connection to the wrong port.

Figure 1-3. Docking station back panel.

4) Connect the tube from the port labeled “EXHAUST” on the rotameter to the port labeled “OUT”

on the back of the docking station.

5) If using the Pearl Clean Box, connect one of the provided HEPA filter assemblies (packaged with

the Clean Box) to the port labeled “IN” on the rotameter. The gender of the connector assures

the filter will be plugged-in in the correct orientation.

Figure 1-4. Docking station rotameter with HEPA

filter assembly installed between the IN port on the

rotameter and the anesthesia gas supply tube from

the vaporizer.

Page 3

Chapter 1: Pearl Docking Station

6) Connect one of the provided HEPAfilter assemblies between the SmartFlow system and the

Induction Chamber.

7) Connect the opposite end of the HEPA filter assembly to the provided anesthesia gas supply tube

and then connect the other end of the anesthesia gas supply tube to one of the output manifold

connectors on the SmartFlow Anesthesia System, or equivalent (Figure 1-5).

Note: For imaging beds not requiring a HEPA filter, connect the anesthesia gas supply tube dir-

ectly to the rotameter. For other vaporizers, it may be necessary to remove the connector that con-

nects to the SmartFlow System and replace it with an appropriate fitting.

CAUTION: The Pearl®ImagingSystem Docking Station is compatible only with anesthesia

systems that supply isoflurane gas. The flow rate and pressure into the instrument should not

exceed 1 liter per minute and 1 psi, respectively. If the pressure exceeds 5 psi, the rotameter

will not accurately show the flow to the nose cone.

ATTENTION:

La station d'amarrage de Pearl n'est compatible qu'avec les systèmes d'anesthésie qui

fournissent le gaz isoflurane. Le débit et la pression dans l'instrument ne devraient pas

excéder 1 litre par minute et 6.9 kPa, respectivement. Si la pression excède l'estimation de

34.5 kPa, le rotamètre ne montrera pas en juste proportion l'écoulement au cône de nez.

EQUIPMENT LEGEND

EX.

OXYGEN TANK

OXYGEN REGULATOR

SMARTFLOW ANESTHESIA SYSTEM

SMARTFLOW VAPORIZER

INDUCTION CHAMBER WITH HEATER

WATER PUMP

PEARL IMAGER

PEARL ROTAMETER

PEARL IMAGER IMAGING BED OR CLEAN BOX

CHARCOAL FILTER CANISTER

HEPA FILTER ASSENBLY (9957-055)

FOR 9930-022 PEARL CLEAN BOX

DOCKING STATION ROTAMETER AND STAND

PEARL DOCKING STATION

10’ TUBING ASSEMBLY (#9957-056) INCLUDING

WITH THE PEARL DOCKING STATION

TUBING ASSEMBLY INCLUDDED WITH

THE PEARL IMAGER

OUT

Figure 1-5. General representation of a typical system consisting of the docking station with the Pearl Imaging System

and SmartFlow Anesthesia System. Docking station rotameter (L) connects to optional HEPA ﬁlter assembly (K) and

anesthesia gas supply tube (N) before connecting to a port on the SmartFlow Anesthesia System (C).

Page 4

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