Millipore Direct Detect Manuale utente

User Guide

Direct Detect®Spectrometer

Made in Germany

The M logo, Millipore, Direct Detect, and Amicon are registered trademarks

of Merck KGaA, Darmstadt, Germany.

All trademarks of third parties are the property of their respective owners.

00000833, Rev. D, 05/14

Notice

The information in this document is subject to change without notice and should not be construed

as a commitment by EMD Millipore Corporation (“Millipore”) or an afﬁliate. Neither EMD Millipore

Corporation nor any of its afﬁliates assumes responsibility for any errors that may appear in this

document.

Contents

1 Introduction............................................................................................................... 1

2 Components Supplied ............................................................................................... 2

3 Symbols Used in this User Guide.............................................................................. 3

4 Product Labeling........................................................................................................ 3

5 Safety Precautions .................................................................................................... 4

6 Installation................................................................................................................. 4

6.1 Packaging ........................................................................................................................................................4

6.2 Site Requirements.........................................................................................................................................5

6.3 Installation Conﬁguration ..........................................................................................................................6

6.4 Unpacking and Setting up the Computer...............................................................................................6

6.5 Unpacking and Setting Up the Direct Detect® Spectrometer...........................................................6

7 Direct Detect® Spectrometer Parts and Functions.................................................. 8

7.1 General Technical Features.........................................................................................................................8

7.2 Direct Detect® Computer and Software .............................................................................................. 10

8 General Guidelines .................................................................................................. 11

8.1 Buffer components .................................................................................................................................... 11

8.2 Materials Required but Not Supplied................................................................................................... 11

9 Direct Detect® Assay-free Card and Sample Preparation..................................... 12

9.1 Direct Detect® Assay-free Card ............................................................................................................ 12

9.2 Sample Preparation ................................................................................................................................... 13

10 How to Use the Direct Detect® Spectrometer....................................................... 14

10.1 Accessing Direct Detect® Software....................................................................................................... 14

10.2 Loading and Installing the Card............................................................................................................. 15

10.3 Measurement .............................................................................................................................................. 16

10.4 Removing the Card .................................................................................................................................... 18

11 Switching from Direct Connection to Intranet Connection (Networking).......... 19

11.1 Reconﬁguration Requirements............................................................................................................... 19

11.2 Changing from Direct Connection to Intranet Connection............................................................ 20

11.3 Changing from Intranet Connection to Direct Connection............................................................ 22

11.4 Changing Computer Network Settings Manually ............................................................................. 24

12 Troubleshooting ....................................................................................................... 26

12.1 General Information.................................................................................................................................. 26

12.2 Problems Indicated by Hardware Diagnostics Icon .......................................................................... 27

12.3 Problems Indicated by Instrument Status Light (top of instrument) .......................................... 28

12.4 Problems Indicated by Instrument Validation Icon .......................................................................... 29

12.5 Problem Indicated by Instrument Internal Humidity Icon.............................................................. 29

13 Maintenance ............................................................................................................ 30

13.1 Replacing the IR Source........................................................................................................................... 30

13.2 Replacing the Desiccant........................................................................................................................... 33

13.3 Cleaning........................................................................................................................................................ 35

13.4 Description of Instrument Validation Tests......................................................................................... 36

13.5 Running the Instrument Validation OQ and PQ Tests...................................................................... 39

13.6 Storage .......................................................................................................................................................... 39

14 Installation, Operation, and Process Qualiﬁcation Protocol (Optional).............. 40

14.1 Installation Qualiﬁcation Protocol (IQ)................................................................................................ 40

14.2 Instrument Hardware Diagnostic Protocol.......................................................................................... 40

14.3 Operation Qualiﬁcation Protocol (OQ)................................................................................................. 42

14.4 Performance Qualiﬁcation Protocol (PQ)............................................................................................ 42

14.5 Protein Measurement Veriﬁcation ........................................................................................................ 42

14.6 Direct Detect® System IQ/OQ/PQ Report Summary......................................................................... 44

15 Speciﬁcations........................................................................................................... 45

16 Ordering Information .............................................................................................. 46

17 Technical Assistance................................................................................................ 47

18 EC Representative.................................................................................................... 47

19 Standard Warranty .................................................................................................. 47

Contents, continued

Direct Detect® Spectrometer User Guide 1

1 Introduction

The Direct Detect® infrared (IR)-based quantiﬁcation system is an innovative combination of software-

controlled instrumentation and EMD Millipore Corporation’s advanced membrane technology, optimized

for biomolecule detection, quantiﬁcation of proteins, and analysis of lipids/detergents. Biomolecules

are applied directly to a card-based hydrophilic polytetraﬂuoroethylene (PTFE) membrane that is

transparent in most of the infrared spectral region.

The Direct Detect® system measures amide bonds in protein chains, accurately quantifying an intrinsic

component of every protein without relying on amino acid composition, dye binding properties, or

redox potential. Protein concentrations from 0.25 to 5 mg/mL can accurately be determined from a

minimal sample volume (2 µL) without bio- or immuno-chemical staining. Sample analysis takes only

minutes and in most cases can be performed directly from the buffered or native solution.

Quantiﬁcation of lipids and detergents requires a user-deﬁned standard curve. Unknown mixtures can

be analyzed by determining the relative IR absorbance.

The system has been validated with proteins and cell lysates representative of those currently in use in

research laboratories.

Direct Detect® Spectrometer User Guide

2 Components Supplied

The Direct Detect® system includes the following components:

Figure 1. Components supplied

1A Direct Detect® spectrometer

1B Instrument power adapter with 4 power cord/plug conﬁgurations (EU, UK, US, Japan)

1C Data cable (crossover, 10Base-T Ethernet with RJ45 connectors)

1D Dell® Netbook computer with Direct Detect® Software installed

1E Computer power adapter with world-wide plug adapter

1F Netbook stand

1G Direct Detect® Assay-free cards (package of 50)

1H Direct Detect® spotting tray

Desiccant pack replacement (1, not shown)

Torx® TX20 screw driver for replacing the IR source (not shown)

Direct Detect® Spectrometer Quick Start Card (not shown)

Direct Detect® Spectrometer User Guide 3

3 Symbols Used in this User Guide

The following symbols are used throughout this user guide and/or on product labels, and the user shall

abide by indicated requirements:

Symbol Deﬁnition

YWarning alerts you to actions that may cause personal injury or pose a

physical threat.

Class I Laser

Product Class I Laser product, complies with 21 CFR Chapter 1, Subchapter J

iRead the documentation

hCatalogue number

fSerial number

MManufacturer

CCE conformity marking. Reference Declaration of Conformity for speciﬁc directives.

Do not discard with common solid waste at end of life. Segregate with other waste

electrical and electronic equipment (WEEE) and send to an appropriate facility for

recycling. For information on recycling electrical and electronic products in the

European Union, please visit www.millipore.com/weee.

Hot Surface

4 Product Labeling

Figure 2. Product label appears on both the back and the bottom of the instrument

Direct Detect® Spectrometer User Guide

5 Safety Precautions

Review and understand the safety precautions below before installing and operating the Direct Detect®

spectrometer.

YWARNING

● To avoid danger of electric shock, do not install the instrument in an area with a high humidity

level. Refer to Site Requirements in section 6.2.

● Do not touch the Ethernet cable or universal power adapter plugs with wet hands.

● To avoid potential shock hazard, choose the correct plug conﬁguration and make sure that the

power adapter is plugged securely into a properly grounded AC power outlet. Make sure that

the connection between the cable and the instrument is secure.

● Always ensure that the power supply input voltage matches the voltage available in your

location.

● Use only the power supplies that were supplied by EMD Millipore Corporation for use with the

Direct Detect® system. Use of other power supplies may damage the system.

● Minimize power draw from other instruments on the same power circuit.

● Do not use with ﬂammable or explosive liquids. Cards that have been spotted with samples in

ﬂammable solvents must be dried prior to insertion into the instrument.

6 Installation

NOTE: For a full installation, operation, and process qualiﬁcation protocol and checklist, refer to

section14.

6.1 Packaging

Inspect the packaging for damage upon receipt. If there are any signs of damage, contact

the responsible shipping company.

YWARNING: Do not put a spectrometer showing signs of damage into operation as this

may result in severe personal injuries and/or property damage.

Keep the original shipping boxes in case you need to return the spectrometer for servicing

or repair.

NOTE: Instruments will not be accepted for repair if not in original packaging. EMD

Millipore Corporation will provide shipping boxes for a charge if original packaging

has been discarded.

Direct Detect® Spectrometer User Guide 5

6.2 Site Requirements

The user must provide an installation site that meets the following requirements:

Space requirement Stable and horizontal base which is able to bear the weight of

at least 8 kilograms (kg)

A clearance of at least 25 centimeters (cm) at the

spectrometer rear side

Environment Temperature range: 18–35 °C

Humidity (non-condensing): less than 35% (relative humidity)

Temperature variation: less than 1 °C/hour

Humidity variation: less than 3%/hour

Do not install devices such as large electric motors, heaters,

welding equipment, radio transmitting equipment, units emit-

ting pulsed NMRs, or high-powered lasers in close proximity

to the Direct Detect® spectrometer. These devices can cause a

spectrometer malfunction.

Make sure that these types of devices are not connected to the

same electrical circuit as the Direct Detect® spectrometer.

Vibration Do not locate the Direct Detect® spectrometer near vibration

sources (centrifuges, vortexes, ventilation hoods, air

conditioners, etc.), or in rooms with intense ﬂoor vibrations.

Inductive electrical

interference

Do not install the Direct Detect® spectrometer near any

sources of potential inductive electrical interference (e.g.,

pumps, switching motors, microwave ovens, etc.), sources of

high energy pulses, or sources that might cause magnetic or

radio frequency interference.

Power supply Voltage range: 100–240 VAC

Frequency range: 50–60 Hz

Protection class: I

The power supply unit automatically adapts to most power

sources commonly used.

Make sure that the power supply is easily accessible at all

times.

Connect the Direct Detect® spectrometer only to a grounded

power outlet.

Take precautions to ensure uninterrupted power supply if there

is the potential for power supply issues (e.g., brownouts, power

surges, frequent thunderstorms, etc.)

Direct Detect® Spectrometer User Guide

6.3 Installation Conﬁguration

The Direct Detect® spectrometer is conﬁgured to operate as a stand-alone unit

connected to the supplied Netbook computer (PC) using the factory-set standard IP

address (10.10.0.1).

6.4 Unpacking and Setting up the Computer

Unpack the Netbook computer, power adapter, and world-wide plug adapter from

the shipping box. Choose the plug adapter with the correct conﬁguration for local

requirements and attach it to the plug. Plug the low voltage (round) end of the power

adapter into the power port of the PC, but do not plug the computer into a power outlet at

this time.

6.5 Unpacking and Setting Up the Direct Detect® Spectrometer

Remove all components from shipping boxes and lay out on work surface.

6.5.1 Positioning and Connecting Components (Figure 3)

1. Place instrument on bench and put Netbook stand on top of instrument. The

front tab of the stand ﬁts into the slot on top of the instrument (5B in Figure5).

2. Place Netbook computer on stand.

Figure 3. Rear side - Connection ports

3. Turn the instrument/computer assembly so that the rear of the instrument is

facing you (Figure 3).

4. Connect one end of the provided Ethernet cable to the instrument ETH/LAN

port (3A) and the other end to the PC network/LAN port (3B).

Questo manuale è adatto per i seguenti modelli

Indice

Altri manuali Millipore Attrezzature da laboratorio

Millipore

Millipore SNAP i.d Manuale utente

Millipore

Millipore MERCK EZ-Pak Manuale utente

Millipore

Millipore ReadyStream Manuale utente

Millipore

Millipore milli-q Manuale utente

Millipore

Millipore Amicon Ultra-4 Series Manuale utente

Millipore

Millipore Steritest Symbio Manuale utente

Millipore

Millipore M Air T Manuale utente

Millipore

Millipore 8003 Manuale utente

Millipore

Millipore PR00SIA1 Manuale per l'uso e la cura

Millipore

Millipore XX4004700 Manuale utente

Millipore

Millipore Amicon Ultra-2 Manuale utente

Millipore

Millipore APC ErgoTouch Pro 2 Manuale utente

Millipore

Millipore Samplicity Manuale utente

Millipore

Millipore Viresolve Pro VPMCPDKNB9 Manuale utente

Millipore

Millipore Milliflex Oasis Manuale utente

Millipore

Millipore Amicon 6028 Manuale utente

Millipore

Millipore Milli-Q Advantage A10 Manuale utente

Millipore

Millipore Helicon S10 Manuale utente

Millipore

Millipore Millicell ERS-2 Manuale utente

Millipore

Millipore Spectroquant Multy Manuale utente

Millipore

Millipore XX11 000 00 Manuale utente

Millipore

Millipore Elix 35 Manuale utente

Millipore

Millipore Millicell MDCI10000 Manuale utente

Millipore

Millipore Labscale Manuale utente

Manuali Attrezzature da laboratorio popolari di altre marche

Qiagen

Qiagen DML 3000 Manuale utente

Queensgate

Queensgate NANOSCAN OP400 Manuale di programmazione

Conviron

Conviron GEN1000-GE Manuale utente

Parr Instrument

Parr Instrument 4560 Manuale di istruzioni

IKA

IKA IC control Manuale utente

Integra

Integra DOSE IT Manuale utente

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manuale utente

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuale utente

NI PXI-5422 Manuale

Collomix

Collomix Aqix Manuale utente

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuale utente

Ocean Insight

Ocean Insight FLAME-NIR+ Manuale utente

Parker

Parker ALIGN-MG-NA Manuale utente

BD 644787 Manuale utente

DENTAURUM

DENTAURUM Compact Megaplus Manuale utente

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuale utente

VWR

VWR SAS Super IAQ Manuale utente

illumina

illumina MiSeqDx Manuale utente