Miltenyi Biotec MACSQuant X Manuale utente

MACSQuant® X

User manual

No part of this user manual may be reproduced, stored in a retrieval system, transmitted, published, or distributed in any form or by any means, electronically,

mechanically, by photocopying, microfilming, recording, or otherwise, without the prior written consent of Miltenyi Biotec; however, notwithstanding the foregoing, the

owners of the MACSQuant® X may make copies solely for purposes of training personnel in the use and servicing of the unit within their business or organization.

Maximal care has been taken by Miltenyi Biotec in the preparation of this user manual. However, Miltenyi Biotec shall not be liable for any technical or editorial errors or

omissions contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this document. The information in this

document is provided “as is” without warranty of any kind, and is subject to change without notice. MACS, MACSima, MACSQuant, MACSQuantify, and the Miltenyi

Biotec logo are either registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide. All other trademarks mentioned in this

document are the property of their respective owners and are used for identification purposes only. Unless otherwise specifically indicated, Miltenyi Biotec products and

services are for research use only and not for therapeutic or diagnostic use.

MACSQuant® X

User manual

Original instructions

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone +49 2204 8306-0

Fax +49 2204 85197

macsde@miltenyi.com

www.miltenyibiotec.com

Read the chapter Important safety information and all

other information in this user manual before using the

instrument

Before using the MACSQuant X, read the chapter Important safety information and all other information

contained in this user manual, including any safety and operating instructions. Pay special attention to all

warnings displayed on the instrument. Failure to read and follow these guidelines could lead to improper or

incorrect usage and result in damage to the instrument. Improper usage could also cause severe personal injury,

death, unpredictable results, instrument malfunction, and premature wear to components shortening the

lifetime of the instrument. Such actions may void your warranty. Keep the user manual and any other safety and

operating instructions provided with the instrument in a safe place accessible to all users for future reference.

If you have a serious concern regarding the safe use of your instrument, contact your authorized Miltenyi Biotec

service provider or call Miltenyi Biotec Technical Support.

Content

Important safety information 7

Wichtige Sicherheitshinweise 15

Información importante de seguridad 23

Informations de sécurité importantes 31

Importanti istruzioni di sicurezza 39

1 Introduction 47

1.1 Key features of the MACSQuant® X 48

2 Installation 49

2.1 Components included in the delivery 49

2.2 Consumables 50

2.3 Accessories 50

2.4 Service contracts 50

2.5 Unpacking the instrument 51

2.6 Assembly 53

2.6.1 Install the fluid bottles 53

2.6.2 Install the Single tube rack 56

2.6.3 Install the MACSQuant X Orbital Shaker 57

2.6.4 Connect the instrument to the power supply 57

2.6.5 Install the webcam 57

3 Operating the instrument 59

3.1 Switching on the instrument 59

3.2 Shutting down 59

3.2.1 Manual shutdown 59

3.2.2 Automatic shutdown 59

3.3 Long-term storage 60

4 Calibration 61

4.1 Calibrating the sample uptake unit 61

4.1.1 Calibrating the robotic needle arm 61

4.1.2 Calibration of the Single tube rack 63

4.1.3 Calibration of the MACSQuant X 5 Rack 63

4.1.4 Calibration of the 96-well plate 64

4.1.5 Calibration of the 384-well plate 64

4.1.6 Calibration of the MACSPlex Filter Plate 65

4.1.7 Calibration of custom 96 and custom 384 racks 65

4.1.8 Calibration of Universal Reagent Rack 65

4.2 Calibrating the rack detection 66

4.2.1 Single tube rack detection 66

4.3 Volume calibration 66

4.4 Tube length calibration 69

5 Hardware monitor 71

5.1 Fluidics 71

5.2 Sample uptake unit 71

5.3 Lasers and detectors 72

6 Live Support 73

7 Maintenance 75

7.1 Decontaminating the instrument 75

7.2 Exchanging fluid bottles 75

7.3 Exchanging hydrophobic air filters 75

7.4 Cleaning the washing station 75

7.5 Cleaning the uptake needle 76

7.6 Exchange the sample needle 76

7.7 Exchange the sample tubing 78

7.8 Exchange the needle sealing 81

8 Troubleshooting 85

8.1 Syringe pump leakage 85

8.2 Air bubbles during measurement / no event acquisition 85

8.3 Excessive debris is present during acquisition 85

8.4 Touchscreen remains dark 85

9 Technical data and specifications 87

9.1 EC / EU Declaration of Conformity 90

9.1.1 Additional international conformity 91

10 Technical support 93

11 Limited warranty 95

Important safety information

The instrument is designed for safe use when installed correctly, operated by trained personnel, operated in

accordance with general safety practices and the instructions in this user manual.

Warnings and precautions

The guidelines in this section explain the potential risk associated with operating this instrument and provide

important supplemental safety information to minimize the risks. Follow the instructions carefully to protect

yourself, others, and the equipment from potential hazards and create a safe work environment. Use this

instrument only as specified by the manufacturer to avoid damage of equipment and injury to personnel.

Always follow local working area safety instructions and laboratory policies as well as standards for health,

safety and prevention of accidents. Contact the local authorities governing electrical power supply, building

constructions, maintenance, or safety for more information about the safe installation and operation of the

instrument.

Hazard levels

Signal words are used to identify safety and property damage messages. The following signal words are used to

throughout this user manual.

or WARNING! indicates a potentially hazardous situation, which, if not avoided, could result in death or serious

injury.

or CAUTION! indicates a potentially hazardous situation, which, if not avoided, may result in minor or

moderate injury. It may also be used to alert against unsafe practices.

Safety symbols

The following symbols are used to highlight conditions that could cause injury to personnel.

Safety alert. Risk of danger. The documentation needs to be consulted in all cases where this safety alert

symbol is used on the instrument, in order to find out the nature of the potential hazard and any actions that

have to be taken.

Risk of electric shock.

Risk of crushing and shearing of bodily parts.

Hazardous optical radiation.

Hazardous laser radiation.

Biohazard. Risk of contamination if potentially dangerous biological material is used.

IMPORTANT SAFETY INFORMATION

DEESFRIT

Protective conductor terminal. Symbol is attached on the inside of the instrument. Information for service

personnel.

On (Power supply).

Off (Power supply).

Safety labels

Please notice the hazard points and safety symbols of the instrument. All safety labels and safety markings must

be kept clean and legible. Inspect the safety labels and safety markings regularly and replace them if not legible

or identifiable from a safe viewing distance. Contact Miltenyi Biotec for replacement labels.

Figure 1: Hazard areas and safety symbols on the front of the MACSQuant X.

Figure 2: Hazard areas and safety symbols on the rearside of the MACSQuant X.

General safety instructions

Safe operation of the instrument is no longer ensured if the instrument is not working properly and/or the

displayed instructions or messages advise you to contact Technical Support. Immediately switch off and unplug

the instrument from the power outlet and contact an authorized Miltenyi Biotec service provider or Miltenyi

Biotec Technical Support.

IMPORTANT SAFETY INFORMATION

DEESFRIT

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