Molecular Devices StakMax Manuale utente

StakMax® Plate Handling System

User Guide

0112-0133 Rev B

May 2010

This document is provided to customers who have purchased Molecular Devices, Inc.

(“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation

of such Molecular Devices equipment, software, reagents, and consumables. This document is

prohibited, except as Molecular Devices may authorize in writing.

Equipment, software, reagents, and consumables that may be described in this document are

protected under one or more patents filed in the United States, Canada, and other countries.

Additional patents are pending.

Software that may be described in this document is furnished under a license agreement. It is

against the law to copy, modify, or distribute the software on any medium, except as specifically

allowed in the license agreement. Furthermore, the license agreement may prohibit the software

from being disassembled, reverse engineered, or decompiled for any purpose.

Portions of this document may make reference to other manufacturers and/or their products,

which may contain parts whose names are registered as trademarks and/or function as

trademarks of their respective owners. Any such usage is intended only to designate those

manufacturers' products as supplied by Molecular Devices for incorporation into its equipment

and does not imply any right and/or license to use or permit others to use such manufacturers'

and/or their product names as trademarks.

Molecular Devices makes no warranties or representations as to the fitness of this equipment for

any particular purpose and assumes no responsibility or contingent liability, including indirect or

consequential damages, for any use to which the purchaser may put the equipment described

herein, or for any adverse circumstances arising therefrom.

For research use only. Not for use in diagnostic procedures.

Equipment built by Molecular Devices, Inc.

1311 Orleans Drive, Sunnyvale, California, United States of America 94089.

Molecular Devices, Inc. is ISO 9001 registered.

The trademarks mentioned herein are the property of Molecular Devices, Inc. or their respective

owners.

0112-0133 Rev B 3

Contents

Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Safety Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Emergency Interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

About this Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

Additional Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

SoftMax® Pro User Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Chapter 2 Installation and Setup of StakMax® system . . . . . . . . . 15

Unpacking the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

Installing the StakMax® Instrument . . . . . . . . . . . . . . . . . . . . . . . . .18

Connecting the Baseplate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

Installing the StakMax® Driver . . . . . . . . . . . . . . . . . . . . . . . . . . . .26

Starting the Software Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Performing a Functional Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Required Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Getting Started. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

Opening the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Closing the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Starting a Read Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Stopping the StakMax® Instrument in an Emergency. . . . . . . . . . .40

Setting Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40

Save Data After Read (AutoSave). . . . . . . . . . . . . . . . . . . . . . . . . . .41

Naming Conventions for AutoSave Files . . . . . . . . . . . . . . . . . . . . .45

AutoSave File Formats. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46

Installing or Replacing a Barcode Reader . . . . . . . . . . . . . . . . . . . . .46

Required Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46

Chapter 3 Installation and Setup of SpectraMax® L instrument . . 49

Contents

4 0112-0133 Rev B

Unpacking the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49

Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Installing the StakMax® Instrument. . . . . . . . . . . . . . . . . . . . . . . . .52

Connecting the Baseplate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57

Installing the StakMax® Driver . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

Starting the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Performing a Functional Check . . . . . . . . . . . . . . . . . . . . . . . . . . . .70

Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Opening the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Closing the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Starting a Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

Stopping the StakMax® Instrument in an Emergency. . . . . . . . . . .72

Setting Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73

Save Data After Read (AutoSave) . . . . . . . . . . . . . . . . . . . . . . . . . .74

Naming Conventions for AutoSave Files . . . . . . . . . . . . . . . . . . . . .78

AutoSave File Formats. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78

Chapter 4 Installation and Setup of the AquaMax® Microplate

Washer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Unpacking the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81

Unpacking the StakMax® Instrument . . . . . . . . . . . . . . . . . . . . . . .82

Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83

Installing the StakMax® Instrument. . . . . . . . . . . . . . . . . . . . . . . . .84

Chapter 5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Starting the StakMax® System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89

Turning the StakMax® Instrument On and Off . . . . . . . . . . . . . . . .89

Opening the StakMax® Software Window . . . . . . . . . . . . . . . . . . .90

Loading the StakMax® Instrument . . . . . . . . . . . . . . . . . . . . . . . . . .90

Continual Feed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94

Magazine Loading and Unloading Tool. . . . . . . . . . . . . . . . . . . . . .95

Single Plate Loading Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104

The StakMax® Software Interface . . . . . . . . . . . . . . . . . . . . . . . . . .104

Start Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105

Stop Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109

Emergency Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110

Load Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110

Return Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110

StakMax® Plate Handling System User Guide

0112-0133 Rev B 5

Restack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110

Demo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111

Scripting (optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111

Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112

Stacker Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112

Barcoding. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

Allow Edit Prompting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

Append Date/Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114

Status Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115

Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115

Chapter 6 Scripting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

Script Editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118

Command Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120

StakMax® Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120

SoftMax® Pro Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120

Advanced Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122

Misc Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123

Creating a New Script from a Template . . . . . . . . . . . . . . . . . . . .123

Adding Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125

Removing Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128

Changing the Order of Commands . . . . . . . . . . . . . . . . . . . . . . . .131

Checking the Syntax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133

Saving Scripts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134

Printing Scripts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135

Using Repeats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135

Chapter 7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137

System Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137

Weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137

As-Needed Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137

Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137

Chapter 8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139

Appendix A System Diagrams and Specifications . . . . . . . . . . . . 141

Performance Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141

Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141

Contents

6 0112-0133 Rev B

General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141

Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142

0112-0133 Rev B 7

Safety

This chapter provides important safety information for the use of the

StakMax®instrument. All users should read and understand the safety

guidelines before operating the StakMax instrument. The following

information is described in this chapter:

•Safety Guidelines on page 7

•Labeling on page 7

•Emergency Interrupt on page 9

Safety Guidelines

Read and understand all of the safety guidelines before using the StakMax

instrument.

Labeling

The following warning labels are attached to the StakMax instrument. Do not

remove these labels.

Safety

8 0112-0133 Rev B

Table S-1 StakMax®instrument labels

Instrument Label Nameplate

Instrument nameplate

StakMax®Identification, voltage,

and serial number (back of

instrument)

StakMax input and output stack

labels

Laser-based barcode reader safety

label (internal)

Power I/O label (right side of

instrument)

CE mark for European and TUV

Rheinland

1311 Orleans Drive, Sunnyvale

CA 94089 408 747-1700

+24V

INPUT

STACK

OUTPUT

STACK

+ 24 VDC RS-232

USB

StakMax® Plate Handling System User Guide

0112-0133 Rev B 9

Emergency Interrupt

The StakMax instrument Power/Reset button is located on the left side of

the front panel. The Power/Reset button operates as a Power On button

and it functions as a Reset button that can be used in case of emergency

(for example, the instrument is about to crash). When pressed while the

instrument is in use, it functions as an Emergency Interrupt button to

immediately stop any StakMax movement and to reset the entire system.

Safety

10 0112-0133 Rev B

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