Molecular Devices StakMax Manuale utente

5016072 B

October 2018

StakMax®

Microplate Handling System

User Guide

StakMax Microplate Handling System User Guide

2 5016072 B

This document is provided to customers who have purchased Molecular Devices equipment, software,

reagents, and consumables to use in the operation of such Molecular Devices equipment, software,

reagents, and consumables. This document is copyright protected and any reproduction of this

document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in

writing.

Software that may be described in this document is furnished under a non-transferrable license. It is

against the law to copy, modify, or distribute the software on any medium, except as specifically

allowed in the license agreement. Furthermore, the license agreement may prohibit the software

from being disassembled, reverse engineered, or decompiled for any purpose.

Portions of this document may make reference to other manufacturers and/or their products, which

may contain parts whose names are registered as trademarks and/or function as trademarks of their

respective owners. Any such usage is intended only to designate those manufacturers’ products as

supplied by Molecular Devices for incorporation into its equipment and does not imply any right and/or

license to use or permit others to use such manufacturers’ and/or their product names as trademarks.

Each product is shipped with documentation stating specifications and other technical information.

Molecular Devices products are warranted to meet the stated specifications. Molecular Devices makes

no other warranties or representations express or implied, including but not limited to, the fitness of

this product for any particular purpose and assumes no responsibility or contingent liability, including

indirect or consequential damages, for any use to which the purchaser may put the equipment

described herein, or for any adverse circumstances arising therefrom. The sole obligation of Molecular

Devices and the customer's sole remedy are limited to repair or replacement of the product in the

event that the product fails to do as warranted.

For research use only. Not for use in diagnostic procedures.

The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not

be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.

Patents: http://www.moleculardevices.com/patents

Product manufactured by Molecular Devices, LLC.

3860 N. First Street, San Jose, California, 95134, United States of America.

Molecular Devices, LLC is ISO 9001 registered.

5016072 B 3

Contents

Safety Information 4

Warnings, Cautions, Notes, and Tips 4

Chapter 1: Introduction 9

Computer Integration 10

Chapter 2: Set Up the StakMax Microplate Handler 11

Package Contents 11

Unpack the Microplate Handler 12

Connect the Microplate Handler to an Instrument 16

Connect Cables 36

Chapter 3: Load the MicroplateHandler 38

Magazine Load/Unload Tool 39

Single Plate Adapter 45

Continuously Load the Microplate Handler 46

Chapter 4: Get Started 47

Aligning the Gripper 48

Gripper Functional Check 60

Chapter 5: StakMax Software 62

Settings 66

Reading Plates 67

Scripting 69

Chapter 6: Maintenance and Troubleshooting 77

Clean the Instrument 77

Status Dialog Error Codes 78

Installing USB Drivers for Windows 7 79

Obtaining Support 80

Appendix A: Instrument Specifications and Diagrams 81

General Specifications for the StakMaxMicroplate Handling System 81

Space Requirements 83

StakMax Microplate Handling System User Guide

4 5016072 B

Safety Information

Information about the safe use of the instrument from Molecular Devices® includes an

understanding of the user-attention statements in this guide, the safety labels on the

instrument, precautions to follow before you operate the instrument, and precautions to

follow while you operate the instrument.

Make sure that everyone involved with the operation of the instrument has:

Received instruction in general safety practices for laboratories.

Received instruction in specific safety practices for the instrument.

Read and understood all Safety Data Sheets (SDS) for all materials being used.

Read and observe all warnings, cautions, and instructions. The most important key to safety

is to operate the instrument with care.

WARNING! If the instrument is used in a manner not specified by Molecular

Devices, the protection provided by the equipment might be impaired.

Warnings, Cautions, Notes, and Tips

All warning symbols are framed within a yellow triangle. An exclamation mark is used for

most warnings. Other symbols can warn of other types of hazards such as biohazard,

electrical, or laser safety warnings as are described in the text of the warning. Follow the

related safety information.

The following user attention statements might be displayed in the text of Molecular Devices

user documentation. Each statement implies the amount of observation or recommended

procedure.

WARNING! A warning indicates a situation or operation that could cause

personal injury if precautions are not followed.

CAUTION! A caution indicates a situation or operation that could cause damage to

the instrument or loss of data if correct procedures are not followed.

Note: A note calls attention to significant information.

Tip: A tip provides useful information or a shortcut, but is not essential to the

completion of a procedure.

Safety Information

5016072 B 5

Symbols on the Instrument

Each safety label found on the instrument contains an alert symbol that indicates the type of

potential safety hazard.

Symbol Indication

Consult the product documentation.

Potential laser hazard.

Potential electrical-shock hazard from a high-voltage source. All safety instructions

must be read and understood before you proceed with the installation, maintenance,

and service of all modules. Power off the instrument and disconnect the power cord

before you do maintenance procedures that require removal of a panel or cover or

disassembly of an interior instrument component.

Potential pinch hazard.

Required in accordance with the Waste Electrical and Electronic Equipment (WEEE)

Directive of the European Union. It indicates that you must not discard this electrical or

electronic product or its components in domestic household waste or in the municipal

waste collection system.

For products under the requirement of the WEEE directive, contact your dealer or

local Molecular Devices office for the procedures to facilitate the proper collection,

treatment, recovery, recycling, and safe disposal of the device.

- - California proposition 65 requires businesses to provide

warnings to Californians about significant exposures to chemicals that cause cancer, birth

defects, or other reproductive harm.

StakMax Microplate Handling System User Guide

6 5016072 B

Electrical Safety

To prevent electrical injuries and property damage, inspect all electrical equipment before

use and report all electrical deficiencies. Contact Molecular Devices technical support for

equipment service that requires the removal of covers or panels.

WARNING! HIGH VOLTAGE. Within the instrument is the potential of an

electrical shock hazard existing from a high voltage source. Read and understand

all safety instructions before you install, maintain, and service the instrument.

To prevent electrical shock, use the supplied power cord and connect to a properly

grounded wall outlet.

1311 Orleans Drive, Sunnyvale

CA 94089 408 747-1700

+24V

The power port is on the right side of the instrument.

+ 24 VDC RS-232

USB

123

Item Description

1 Power port for connection to the power supply

2 USB port for connection to the computer

3 RS-232 serial port for connection to a microplate washer

To ensure sufficient ventilation and provide access to disconnect power from the

instrument, maintain a 20cm to 30cm (7.9in. to 11.8in.) gap between the rear of the

instrument and the wall.

Power off the instrument when not in use.

Safety Information

5016072 B 7

Laser Safety

WARNING! LASER LIGHT. This symbol indicates that a potential hazard to

personal safety exists from a laser source. When this symbol displays in this

guide, follow the specific safety information related to the symbol.

The StakMax®Microplate Handling System is rated a Class 2 Laser Product because it can

house an optional bar code reader with an embedded laser module.

WARNING! LASER LIGHT. Operate the instrument only when all the doors and

panels of the instrument are in place and closed.

The barcode reader has an embedded Laser Class 2 Laser module and the following

specifications.

Item Description

Lasertype Diode laser

Wavelength 630nm to 680nm

Maximumoutputpower 1.0mW

Laser class Class2 (IEC60825-1, ed. 2.0:2007)

Chemical and Biological Safety

Normal operation of the instrument can involve the use of materials that are toxic,

flammable, or otherwise biologically harmful. When you use such materials, observe the

following precautions:

Handle infectious samples based on good laboratory procedures and methods to

prevent the spread of disease.

Observe all cautionary information printed on the original containers of solutions before

their use.

Dispose of all waste solutions based on the waste disposal procedures of your facility.

Operate the instrument in accordance with the instructions outlined in this guide, and

take all the required precautions when using pathological, toxic, or radioactive materials.

Splashing of liquids can occur. Take applicable safety precautions, such as using safety

glasses and wearing protective clothing, when working with potentially hazardous

liquids.

Observe the applicable cautionary procedures as defined by your safety officer when

using hazardous materials, flammable solvents, toxic, pathological, or radioactive

materials in or near a powered-up instrument.

StakMax Microplate Handling System User Guide

8 5016072 B

Moving Parts Safety

The instrument contains moving parts that can cause injury. Under normal conditions, the

instrument is designed to protect you from these moving parts.

WARNING! If the instrument is used in a manner not specified by Molecular

Devices, the protection provided by the equipment might be impaired.

To prevent injury:

Never try to exchange labware, reagents, or tools while the instrument is operating.

Never try to physically restrict the moving components of the instrument.

Keep the instrument work area clear to prevent obstruction of the movement.

Note: Observe all warnings and cautions listed for all external devices attached to or in

use during the operation of the instrument. See the applicable user guide for the

operating and safety procedures of that device.

5016072 B 9

Chapter 1: Introduction

The StakMax Microplate Handling System is an integrated microplate handler for use with

Molecular Devices microplate readers and microplate washers to provide simple, powerful,

walk-away benchtop automation.

The StakMaxMicroplate Handling System is compatible with the following instruments:

Gemini™EM Microplate Reader

Gemini™ XPS Microplate Reader

SpectraMax®i3x Multi-Mode Microplate Reader

SpectraMax® iD3 Multi-Mode Microplate Reader

SpectraMax® iD5 Multi-Mode Microplate Reader

SpectraMax®L Microplate Reader

SpectraMax®M2 Multi-Mode Microplate Reader

SpectraMax® M2e Multi-Mode Microplate Reader

SpectraMax®M3 Multi-Mode Microplate Reader

SpectraMax®M4 Multi-Mode Microplate Reader

SpectraMax®M5 Multi-Mode Microplate Reader

SpectraMax® M5e Multi-Mode Microplate Reader

SpectraMax®Paradigm® Multi-Mode Microplate Reader

Newer models of the following instruments are compatible with the StakMaxMicroplate

Handling System:

SpectraMax®190 Absorbance Microplate Reader (Serial number begins with NNR)

SpectraMax®340PC384 Absorbance Microplate Reader (Serial number begins with LNR)

SpectraMax®Plus384 Absorbance Microplate Reader (Serial number begins with MNR)

VersaMax™ Microplate Reader (Serial number begins with BNR)

The StakMaxMicroplate Handling System is compatible with the following microplate

washers:

AquaMax®2000 Microplate Washer

AquaMax®4000 Microplate Washer

StakMax Microplate Handling System User Guide

10 5016072 B

Computer Integration

Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro

Data Acquisition and Analysis Software that you install on the computer that you use to

operate the instrument. The SoftMax Pro Software provides integrated instrument control,

data display, and statistical data analysis.

You should install the SoftMax Pro Software on the computer before you set up the

instrument. Please be aware that some updates to the SoftMax Pro Software require a

purchase. Contact Molecular Devices before you update the software. To download the

latest version of the software, visit:

https://www.moleculardevices.com/products/microplate-readers/acquisition-and-analysis-

software/softmax-pro-software#Order.

Note: For information about the computer specifications that are required to run the

software, the software installation and licensing instructions, and the directions to

create the software connection between the computer and the instrument, see the

SoftMax Pro Data Acquisition and Analysis Software Installation Guide.

To prevent data loss, turn off all sleep and hibernation settings for the hard disk, the CPU,

and the USB ports. Disable automatic Windows updates. Update Windows manually when

you do not use the computer to control an instrument. You can set these options in

Windows Control Panel.

When you connect the microplate handler to a compatible microplate reader, the StakMax

Software runs with the SoftMax Pro Software that controls the microplate reader.

When you connect the microplate handler to the AquaMax Microplate Washer, the

AquaMax Software controls the StakMaxMicroplate Handler. See the AquaMax 2000/4000

Microplate Washer User Guide.

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