Multi Channel Systems MEA Series Manuale utente

MEA Amplifier

for Inverse Microscopes

Manual

Information in this document is subject to change without notice.

No part of this document may be reproduced or transmitted without the express written

permission of Multi Channel Systems MCS GmbH.

While every precaution has been taken in the preparation of this document, the publisher

and the author assume no responsibility for errors or omissions, or for damages resulting

from the use of information contained in this document or from the use of programs and

source code that may accompany it. In no event shall the publisher and the author be liable

for any loss of profit or any other commercial damage caused or alleged to have been caused

directly or indirectly by this document.

Printed: 06. 11. 2012

Multi Channel Systems

MCS GmbH

Aspenhaustraße 21

72770 Reutlingen

Germany

Phone +49-71 21-90 92 5 - 0

Fax +49-71 21-90 92 5 -11

sales@multichannelsystems.com

www.multichannelsystems.com

Microsoft and Windows are registered trademarks of Microsoft Corporation. Products that

are referred to in this document may be either trademarks and/or registered trademarks of

their respective holders and should be noted as such. The publisher and the author make

no claim to these trademark.

Table of Contents

Introduction 5

About this Manual 5

Important Information and Instructions 7

Operator's Obligations 7

Guarantee and Liability 7

Important Safety Advice 8

First Use of the MEA Amplifier 9

Welcome to the MEA Amplifier 9

Setting Up and Connecting the MEA Amplifier 10

First Tests and Tutorial 11

First Functional Tests 11

General Performance / Noise Level 11

Operating the MEA Amplifier 13

Signal Amplification and Filters 13

Temperature Control 14

Mounting the MEA Probe and Grounding the Bath 15

Stimulation 17

Service and Maintenance 18

Troubleshooting 19

About Troubleshooting 19

Noise on Single Electrodes 19

Unsteady Baseline 20

Artifacts on All Channels 21

Liquid Spilled onto Amplifier 22

Technical Support 22

Appendix 23

Technical Specifications 23

MEA1060-Inv 23

Heating Element 24

Test Model Probe 24

Pin Layout 25

Pin and MEA Layout 25

Contact Information 27

Ordering Information 27

Index 33

1 Introduction

1.1 About this Manual

This manual comprises all important information about the first installation of the hardware

and software, and about the daily work with the instrument. It is assumed that you have already

a basic understanding of technical and software terms. No special skills are required to read this

manual.

If you are using the device for the first time, please read the important safety advice before

installing the hardware and software, where you will find important information about the

installation and first steps.

The printed manual and Help are basically the same, so it is up to you which one you will use.

The Help offers you the advantage of scrolling through the text in a non-linear fashion, picking

up all information you need, especially if you use the Index, and the Search function. If you are

going to read larger text passages, however, you may prefer the printed manual.

The device and the software are part of an ongoing developmental process. Please understand

that the provided documentation is not always up to date. The latest information can be found

in the Help. Check also the MCS web site (www.multichannelsystems.com) for downloading

up-to-date manuals and Help files.

2 Important Information and Instructions

2.1 Operator's Obligations

The operator is obliged to allow only persons to work on the device, who

 are familiar with the safety at work and accident prevention regulations and have been

instructed how to use the device;

 are professionally qualified or have specialist knowledge and training and have received

instruction in the use of the device;

 have read and understood the chapter on safety and the warning instructions in this manual

and confirmed this with their signature.

It must be monitored at regular intervals that the operating personnel are working safely.

Personnel still undergoing training may only work on the device under the supervision

of an experienced person.

2.2 Guarantee and Liability

The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply.

The operator will receive these no later than on conclusion of the contract.

Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests

and data generated by the use of the device or the software. It is up to the user to use good

laboratory practice to establish the validity of his findings.

Guarantee and liability claims in the event of injury or material damage are excluded when

they are the result of one of the following.

 Improper use of the device.

 Improper installation, commissioning, operation or maintenance of the device.

 Operating the device when the safety and protective devices are defective and/or inoperable.

 Non-observance of the instructions in the manual with regard to transport, storage, installation,

commissioning, operation or maintenance of the device.

 Unauthorized structural alterations to the device.

 Unauthorized modifications to the system settings.

 Inadequate monitoring of device components subject to wear.

 Improperly executed and unauthorized repairs.

 Unauthorized opening of the device or its components.

 Catastrophic events due to the effect of foreign bodies or acts of God.

MEA Amplifier for Inverse Microscopes Manual

2.3 Important Safety Advice

Warning: Make sure to read the following advice prior to install or to use the device and the

software. If you do not fulfill all requirements stated below, this may lead to malfunctions or

breakage of connected hardware, or even fatal injuries.

Warning: Obey always the rules of local regulations and laws. Only qualified personnel should

be allowed to perform laboratory work. Work according to good laboratory practice to obtain

best results and to minimize risks.

The product has been built to the state of the art and in accordance with recognized safety

engineering rules. The device may only

 be used for its intended purpose;

 be used when in a perfect condition.

 Improper use could lead to serious, even fatal injuries to the user or third parties and damage

to the device itself or other material damage.

Warning: The device and the software are not intended for medical uses and must not

be used on humans.

Malfunctions which could impair safety should be rectified immediately.

High Voltage

Electrical cords must be properly laid and installed. The length and quality of the cords must

be in accordance with local provisions.

Only qualified technicians may work on the electrical system. It is essential that the accident

prevention regulations and those of the employers' liability associations are observed.

 Each time before starting up, make sure that the mains supply agrees with the specifications

of the product.

 Check the power cord for damage each time the site is changed. Damaged power cords should

be replaced immediately and may never be reused.

 Check the leads for damage. Damaged leads should be replaced immediately and may never

be reused.

 Do not try to insert anything sharp or metallic into the vents or the case.

 Liquids may cause short circuits or other damage. Keep the device and the power cords always

dry. Do not handle it with wet hands.

Requirements for the installation

 Make sure that the device is not exposed to direct sunlight. Do not place anything on top of

the device, and do not place it on top of another heat producing device. Never cover the device,

not even partially, so that the air can circulate freely. Otherwise, the device may overheat.

 Use and keep the device only in a dry environment. Fluids or damp air may damage or destroy the

device. Spilled liquid can damage or even completely destroy the electronics of the MEA amplifier.

Avoid it by all means.

Warning: The device must not get in contact with fluids! Spilled liquid can damage or even

completely destroy the electronics of the amplifier! This is eminently important when using

a perfusion system. Take care that the flow rates of the inlet and outlet flow match so that

flooding of the amplifier is efficiently prevented.

3 First Use of the MEA Amplifier

3.1 Welcome to the MEA Amplifier

Raw data from up to 60 electrodes of a microelectrode array (MEA) is amplified

by 60 channels of filter amplifiers that are built very small and compact using SMD

(Surface Mounted Devices) technology. The small-sized MEA amplifier combines the

interface to the MEA probe with the signal filtering and the amplification of the signal.

The compact design reduces line pick up and keeps the noise level down.

The MEA sensor is placed directly into the small-sized MEA amplifier. When the amplifier

is closed, the contact pins in the lid of the amplifier are pressed onto the MEA contact pads.

The very close location of the amplifier to the MEA sensor is very favorable concerning a high

signal-to-noise ratio.

MEA amplifiers can be ordered with different gain and bandwidth configurations by the user's

choice. For example, typical pass bands would be 1 to 300 Hz for recording field potentials and

300 to 3000 Hz for recording action potentials. It is also possible to use a broadband amplifier

and filter the data with the digital filter of the free MC_Rack program. Gain settings from

100 to 5000 are possible.

The amplifier is connected to the data acquisition computer via a single 68-pin MCS standard

cable. The analog output signals of the MEA amplifier are then acquired and digitized by the

MC_Card (MEA-System) or your custom data acquisition system.

MEA amplifiers have an integrated heating system for controlling the MEA's temperature.

The desired temperature can be easily programmed with a temperature controller. You will not

need an incubator during recording all environmental conditions are reliably controlled directly

in the MEA dish.

You can then simply ground bad or unwanted electrodes by toggling small switches on the

amplifier. You can also connect a stimulus generator to any electrode(s) for stimulation.

Amplifier inputs are grounded during stimulation to prevent a saturation of the amplifier.

MEA Amplifier for Inverse Microscopes Manual

3.2 Setting Up and Connecting the MEA Amplifier

Warning: Spilled liquid can damage or even completely destroy the electronics of the MEA

amplifier. Please be extremely careful when setting up your perfusion system and when starting

the perfusion. Take care that the flow rates of the inlet and outlet flow match so that flooding

of the amplifier is efficiently prevented.

Note: The housing of the MEA amplifiers was optimized for Zeiss Axiovert microscopes. MEA

amplifiers are compatible to most standard microscopes if the following prerequisites are met.

This MEA amplifier type has been developed for standard inverse microscopes with a rectangular

microscope table. The focal plane of the microscope has to be in a distance of 3.5 mm to the

microscope table.

Note: If you use a complete MEA-System, the MEA amplifier will usually be powered by the

isolated power supply (IPS10W) that is integrated into the data acquisition computer. The power

is distributed along the MCS 68-pin MCS high grade cable. If you use your MEA amplifiers

together with a MEA Switch, or if you use a custom data acquisition system without IPS10W,

you will need an external power supply for operating the amplifier(s). Please ask your local

retailer for more information on setup options.

Note: If you use an USB-ME-System you need an external power supply, for example a PS40W,

for operating the amplifier(s). Please ask your local retailer for more information on setup

options.

Using the MC_Card for data acquisition: Connect the MEA amplifier's output to the MC_Card

input labeled MC_Card Ch. 01–64 with a 68-pin MCS standard cable. If you have a MEA120-System,

connect the second MEA amplifier with a 68-pin MCS standard cable to the input for channels

65 –128 labeled MC_CX64 Ch. 65 –128. If you are using a MEA Switch, connect the amplifiers to

the MEA Switch inputs, and the MEA Switch outputs to the MC_Card.

Using an USB-ME data acquisition device: Connect the MEA amplifier's output to the USB-ME

input via 68-pin MCS standard cable. If you are using a MEA Switch, connect the amplifiers to

the MEA Switch inputs, and the MEA Switch outputs to the USB-ME device.

1. Place the MEA amplifier onto the microscope table.

2. Connect the internal heating element to a Temperature Controller's output channel (D-Sub9

socket) with the black cable. (The Temperature Controller is not part of the standard scope of

delivery, but is included in the MEA-System or USB-ME-System, and can also be ordered

separately.) Do not connect the black heating element cable to the data acquisition computer.

3. You should use a Faraday cage or appropriate materials, for example aluminum foil, for shielding

the amplifier. The shielding should be connected to the amplifier's ground, for example, to the

screws of the cover.

Please see also the separate handout "MEA Microelectrode Systems" for setup suggestions with

detailed illustrations.

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