MyGo Mini Manuale utente
USER GUIDE
PERSONAL PRECISION
MyGo
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WELCOME
Thank you for choosing the MyGo Mini.
The MyGo Mini is the world’s most compact
real-time PCR instrument, with no moving parts
providing silent operation and maximum reliability.
With outstanding precision, it is capable of providing
great results over a broad range of applications.
Personal precision... SETUP
Learn how to install your
MyGo Mini - from setting it
up on your workbench, to
configuring the instrument
and installing the software.
TIPS
Learn some useful tips when
using your instrument.
EXPERIMENT
Learn the basics of setting
up an experiment on your
MyGo Mini.
ANALYSIS
Learn how to easily
analyse your results.
EXPORT
Learn how to export your data
for use with other software.
MAINTENANCE
Learn how to look after
your MyGo Mini.
TROUBLESHOOTING
If you ever have a problem
with your instrument, learn
how to troubleshoot it here.
CONTENT
This quick start guide will teach you what you need
to know to start running your MyGo Mini. It describes
everything from opening and closing the lid, to data
analysis for your qPCR experiments.
Contents include...
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1. MYGO MINI QPCR INSTRUMENT
2. MYGO MINI USER GUIDE
3. POWER SUPPLY UNIT AND MAINS CABLE
4. ETHERNET CABLE
5. USB DRIVE
6. USB DRIVE EXTENSION CABLE
SETUP
INSTALLING YOUR MYGO MINI
You should have the following items in the MyGo Mini box:
Please keep the box the MyGo Mini came in, in case you need to
protect your MyGo Mini during storage or shipment in future.
Heatsink
Lid
Foot
Display LEDs
LID
The lid of the MyGo Mini
can be removed from the
instrument and must be
attached at all times when
not in use. To remove the
lid push down and turn
anticlockwise. To attach
the lid, first line up the lugs
on the lid with the grooves
on the mount plate as
shown below. Once aligned
press down, and then turn
clockwise to secure until you
feel a hard stop.
If the instrument is
connected to power the
instrument will say “Lid on”
and “Lid off” when you attach
and remove the heated lid.
The instrument will display
green when the lid is fitted,
and display pulsating yellow
when the lid is removed.
The lid may get warm during
operation; this is normal.
HEATSINK
This is the outer body of
the instrument and enables
efficient cooling of the
system during thermal
cycling. Ensure that there
is free airflow around the
instrument - do not cover
or wrap the instrument,
and keep it at least 5 cm
away from objects or walls.
FOOT
The foot should always be
placed facing down on your
worktop during operation.
Grooves
Lugs
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USB PORT
MYGO MINI CONNECTIONS
If you turn your MyGo Mini upside down you will see
three connections as shown below:
Insert the USB drive here
to run an experiment from
the USB drive, or to update
firmware using a USB drive.
You can use the supplied USB
drive extension cable.
This is for connecting your
MyGo Mini to your LAN or
computer.
USB Port
Ethernet Port
Power
ETHERNET PORT
POWER
This is to connect your MyGo Mini to the provided power supply unit.
Connect one end of
the ethernet cable to
the instrument and the
other end to a LAN port,
or directly to your computer.
Once your MyGo Mini is
connected to a LAN, you can
connect to your instrument via
Wi-Fi if your network supports it.
POWERING ON YOUR MYGO MINI
Connect the power supply to the MyGo Mini, place
the MyGo Mini on your lab bench, and then connect
AC power to the power supply unit. Your instrument
will now turn on after a few seconds. The display LEDs
will light up multi-coloured, and then turn green if a lid
is attached, or yellow if not. Your MyGo Mini is ready
to run.
CONNECTING
YOUR MYGO MINI
TO A NETWORK,
PC OR LAPTOP
A summary of these connections
is shown on the following page...
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MyGo Mini Connection Modes
1. Direct network connection
2. Local area network connection
3. USB drive connection
MYGO MINI LIGHTS AND SOUNDS
The MyGo Mini uses display LEDs and sounds to
help you understand what the instrument is doing.
Here is a summary of these display LEDs and sounds:
DISPLAY LEDs
SOUNDS
Green Idle / Ready to run
Yellow Lid off
Blue Experiment is starting/
cooling down
Blue Running, low temperature
Pink Running, medium temperature
Red Running, high temperature
Multi-Coloured Experiment complete
Red-Flashing Fault detected
“Lid On” The lid has been attached
“Lid Off” The lid has been removed
“Analysis Starting” An experiment has begun
“Analysis Complete” An experiment has been
completed
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INSTALLING YOUR
MYGO MINI SOFTWARE
Your MyGo USB drive contains software for Windows,
Mac OS X and Linux operating systems. Please open
the software le matching your chosen operating
system. The latest version of the software can also be
downloaded from our website mygopcr.com.
WINDOWS
Double-click on the
windows installer and follow
the on-screen instructions
to install your MyGo Mini
software on Windows.
MAC OS X
Double-click on the
disk image and follow
the on-screen instructions
to install your MyGo Mini
software on your Mac PC.
CONFIGURING YOUR MYGO MINI
With your MyGo Mini software open and your
MyGo Mini connected to the network please connect
to your MyGo Mini Instrument.
Please select Configuration
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To add your new MyGo Mini
select the Add button. You
will now be presented with
a list of available instruments.
Please select MyGo Mini
Double-click on the
instrument you wish to
connect to, or press Select
with the instrument selected.
The instrument will now
be added to the list of
Registered Instruments
for use in the software.
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Intensity (RFU)
Cycle
TIPS
Here are some great tips to keep in mind whilst running your
MyGo instrument.
1. LID GETS WARM
2. DO NOT LEAVE THE LID OFF
3. SPIN YOUR TUBES
4. REMOVE ALL BUBBLES
5. KEEP YOUR LAB CLEAN
6. KEEP YOUR INSTRUMENT CLEAN
7. PC SETTINGS
LID GETS WARM
The MyGo Mini lid can get warm during operation, this is
completely normal.
SPIN YOUR TUBES
This will ensure all well contents are at the bottom of the
wells and will improve performance.
REMOVE ALL BUBBLES
Bubbles can cause optical artefacts as shown in the graph
below. Ensure that no bubbles are present in reaction volumes.
KEEP YOUR LAB CLEAN
Please keep your work space clean including all lab equipment
like surfaces, pipettes, and tube racks. This will keep the
instrument clean and help maintain good results.
KEEP YOUR INSTRUMENT CLEAN
We recommend a routine cleaning of your instrument. To do
so follow the cleaning guide in the Maintenance section.
PC SETTINGS
Please disable all power saving settings e.g. sleep and hibernate.
DO NOT LEAVE THE LID OFF
If the lid is off dust may fall into the wells and affect the
performance of your instrument.
Artefact
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CREATE A NEW EXPERIMENT
EXPERIMENT
This section will teach you everything you need to know
to get started with MyGo Mini experiments. You will
learn how to create, save, open and close experiments.
You will also learn how to set up an experiment including
thermal prole, sample information and optical settings.
Finally we will show you how to run your new experiment
from the software and from the USB drive provided.
Below is the MyGo mini home screen.
Click New to create
a new experiment.
EXPERIMENT SUMMARY
Once you have created a new experiment the
Experiment Summary will be displayed as shown below:
By default the experiment
name will be “New
Experiment” with a date
and time stamp, which
can be edited.
The summary will give
you information about the
instrument you are running,
the settings chosen and any
useful notes you choose to
add about your experiment.
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The Files of Type drop down
menu gives you the option to
open an experiment normally
or as a template that can be
used for a new run.
OPEN AN EXPERIMENT
By selecting Open you can open an experiment
as shown below:
FILES OF TYPE
EXPERIMENT FILE
An experiment file will contain
all the data and analyses but
can not be re-run.
To open as an Experiment
File select the first option
shown on the opposite page.
EXPERIMENT FILE
AS TEMPLATE
Opening an experiment file
as a template allows you to
use the same profile, samples,
settings and analyses to
re-run a previous experiment,
generating new data. When an
experiment file is opened as
a template, it has the same:
1. Temperature profile.
2. Optical settings.
3. Samples and targets.
4. Analyses, including settings.
If you wish to open an
Experiment File as Template
select the second option.
USB RUN
To open a USB run navigate to the USB location using the
Open dialog window shown to the left. Select Experiment File
in the Files of Type drop down menu and then double click on
the USB run.
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