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OpenSPR 1-Channel Operation Manual V4.1.01
Table of Contents
SAFETY AND PREPARATION FOR USE ............................................................................................. 4
1GETTING STARTED .................................................................................................................. 7
1.1 Initial Equipment Setup.................................................................................................... 7
1.1.1 Before any steps are taken ....................................................................................... 7
1.1.2 Standard Equipment Supplies................................................................................... 7
1.1.3 Setting up the OpenSPR............................................................................................ 8
1.1.4 Connecting and Turning on the OpenSPR............................................................... 10
1.2 OpenSPR Software Setup............................................................................................... 11
1.2.1 Minimum Computer Requirements........................................................................ 11
1.2.2 Installing OpenSPR Software .................................................................................. 11
1.3 TraceDrawer Software Setup......................................................................................... 12
1.3.1 Installing TraceDrawer............................................................................................ 12
1.3.2 TraceDrawer License Activation ............................................................................. 12
2STANDARD OPERATING PROCEDURES ................................................................................. 14
2.1 Preparation for your Experiment................................................................................... 14
2.1.1 Required Items........................................................................................................ 14
2.1.2 Fluidic Setup............................................................................................................ 14
2.1.3 Optical Setup........................................................................................................... 16
2.2 Beginning a Test ............................................................................................................. 17
2.3 Instrument Setup ........................................................................................................... 18
2.3.1 Naming your Running Buffers................................................................................. 19
2.3.2 Step 1: Fluidic Priming ............................................................................................ 20
2.3.3 Step 2: Optical References...................................................................................... 23
2.4 Sensor Setup................................................................................................................... 27
2.4.1 Step 3: Install Sensor Chip ...................................................................................... 27
2.4.2 Step 4: Bubble Removal and Prevention ................................................................ 33
2.5 Running an Experiment.................................................................................................. 35
2.5.1 Test Name ............................................................................................................... 35
2.5.2 Experiment Data Interface and Data Acquisition ................................................... 35
2.5.3 Changing the Running Buffer.................................................................................. 37
