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rizing

Micr

ECLIPSE

E400

POL

Instructions

Thank

you

for

purchasing

the

Nikon

products.

This

instruction

manual

is

written

for the

users

of

the

Nikon

Polarizing

Microscope

ECLIPSE

E400

POL.

To

ensure

correct

usage,

read

this

manual

carefully

before

operating

the

instrument

е

It

is

prohibited

to

reproduce

or

transmit

this

manual

in

part

or

whole

without

Nikon's

expressed

permission.

*

The

contents

of

this

manual

are

subject

to

change

without

notice.

*

Some

of

the

products

described

in

this

manual

may

not

be

included

in

the

set

you

have

purchased.

*

Although

every

effort

has

been

made

to

ensure

the

accuracy

of

this

manual,

if

you

note

any

points

that

are

unclear

or

incorrect,

contact

your

nearest

Nikon

representative.

m

Warning/Caution

Symbols

Used

in

This

Manual

us

ein

Although

Nikon

products

are

designed

to

provide

you

with

the

utmo:

lety

during

use,

incorrect

usage

or

disregard

of

the

instructions

can

cause

personal

injury

or

property

damage.

For

your

safety,

read

the

instruction

manual

carefully

and

thoroughly

before

using

the

instrument.

Do

not

discard

this

manual

but

keep

it

near

the

product

for

easy

reference.

In

this

manual,

safety

instructions

are

indicated

with

the

symbols

shown

below.

Вс

sure

to

follow

the

instructions

indicated

with

these

symbols

to

ensure

correct

and

safe

operation.

Symbol

Meaning

WARNING

—

Distearding

instructions

marked

with

this

symbol

may

lead

to

death

or

serious

injury.

A

CAUTION

—

Disreganding

instructions

marked

with

this

symbol

may

lead

to

injury

or

property

damage.

Meaning

of

Symbols

Used

on

the

Equipment

Symbol

Meaning

Caution

for

heat.

A

This

marking

on

the

lamp

cover

calls

your

attention

on

the

following:

*

Lam

cover

becomes

very

hot

during

and

immediately

after

the

illumination.

©

Risk

of

burns.

Do

not

touch

the

lamp

cover

during

and

immediately

after

the

illumination.

•

Make

sure

that

the

lamp

cover

is

sufficiently

cool

before

the

lamp

replacement.

Z\warninc

1.

Intended

product

use

This

microscope

should

only

be

used

for

microscopic

observation.

Do

not

use

it

for

any

other

purpose.

.

Do

not

disassemble

Disassembling

may

cause

malfunction

and/or

electrical

shock.

Do

not

disassemble

any

parts

other

than

those

mentioned

in

this

manual.

If

you

notice

any

malfunction,

notify

your

nearest

Nikon

representative.

Input

voltage

Make

sure

that

the

input

voltage

indicated

on

the

back

panel

of

the

microscope

is

the

same

as

your

regional

voltage.

If

not

the

same.

do

not

use

the

microscope;

instead,

notify

your

nearest

Nikon

representative

immediately.

If

the

microscope

is

used with

the

wrong

input

voltage,

a

short

circuit

or

fire

may

result,

causing

the

damage

of

the

microscope.

Power

cord

To

prevent

electrical

shock,

always

turn

off

the

power

switch

(flip

it

to

the

ide)

before

connecting

or

disconnecting

the

power

cord,

Use

only

the

following

power

supply

cord

set.

Using

the

wrong

power

cord

could

result

in

damage

or

fire.

*

For

100-120V

area

UL

Listed,

detachable

cord

set,

3

conductor

grounding

type

SVT,

No.

18

AWG

rated

at

125V.

7A

minimum.

In

case

of

using

the

extension

cord,

use

only

the

power

supply

cord

with

the

PE

(protective

earth)

wire.

*

For

220-240V

area

3

pole

power

supply

cord

set,

which

must

be

approved

according

to

EU/EN

standards.

Class

I

equipment

should

be

connected

to

PE

(protective

carth)

terminal.

In

case

of

using

the

extension

cord,

use

only

the

power

supply

cord with

the

PE

(protective

earth)

wire.

.

Heat

from

the

light

source

The

lamp

and

the

lamp

cover

become

extremely

hot.

To

avoid

burns,

do

not

touch

the

lamp

cover

while

the

lamp

is

lit

or

for

thirty

minutes

after

it

has

been

turned

off.

Furthermore,

in

order

to

avoid

the

risk

of

fire,

do

not

place

fabric,

paper

or

highly

flammable

materials

such

as

gasoline,

petroleum

benzine,

paint

thinner

or

alcohol

near

the

lamp

cover

while

it

is

lit

or

for

about

thirty

minutes

after

it

has

been

turned

off.

The

back

of

the

microscope

also

becomes

hot

during

use.

Although

this

is

not

a

malfunction,

be

careful

not

to

touch

the

back

of

the

microscope

when

it

is

hot.

[caution

1.

Only

use

the

specified

halogen

lamp

The

power

supply

unit

in

the

microscope

provides

the

power

for

the

halogen

lamp

that

serves

as

the

microscope

light

source.

This

unit

can

supply

power

for

a

halogen

lamp

of

up

to

6V-30W.

The

specified

lamp

must

be

used,

otherwise,

the

microscope

could

be

damaged.

Specified

lamp:

6V-30W

halogen

lamp

(PHILIPS

5761)

.

Precautions

for

replacing

the

lamp

To

prevent

burns,

allow

the

lamp

to

cool

for

at

least

thirty

minutes

after

turning

off

the

power

switch

before

replacing

it.

Furthermore,

to

prevent

electrical

shock

and

damage

to

the

microscope,

always

turn

off the

power

switch

(flip

it

to

the

“O”

side)

and

unplug

the

power

cord

before

replacing

the

lamp.

After

replacing

the

lamp,

be

sure

to

attach

the

lamp

cover

securely.

Never

light

the

lamp

without

its

cover.

.

Do

not

wet

the

microscope

If

the

microscope

gets

wel,

a

short

circuit

may

result

that

could

damage

it

or

make

it

extremely

hot.

If

you

accidentally

spill

a

liquid

on

the

microscope,

immediately

turn

off

the

power

switch

(flip

it

to

the

"O"

side)

and

unplug

the

power

cord.

Then

use

a

dry

cloth

to

wipe

away

the

moisture.

If

any

liquid

gets

inside

the

microscope,

do

not

use

it;

instead,

notify

your

nearest

Nikon

representative.

Weak

electromagnetic

waves

This

microscope

emits

weak

electromagnetic

waves,

The

accuracy

of

any

precision

electronic

equipment

may

be

adversely

affected

if

positioned

too

close.

If

the

microscope

affects

TV

or

radio

reception,

move

the

radio

or

TV

further

away

from

the

microscope.

.

Precautions

for

installation

Be

careful

not

to

pinch

your

hands

or

fingers

when

installing

the

microscope.

This

microscope

is

a

precision

optical

instrument,

and

using

or

storing

it

under

unsuitable

conditions

may

damage

it

or

may

have

an

adverse

effect

on

its

accurac:

е

"Installation

location"

on

p.28

and

always

use

the

microscope

in

a

suitable

environment.

Precautions

for

moving

the

microscope

Securely

hold

the

microscope

by

the

base

of

the

arm

from

the

back.

Do

not

grasp

the

focus

knobs,

eyepiece

tube,

stage,

etc.

when

carrying

the

microscope.

This

could

cause

the

part

to

come

off,

resulting

in

damage

to

the

microscope.

Be

careful

not

to

hit

the

lamp

cover

against

the

other

objects

when

lifting

up

or

placing

the

microscope.

This

microscope

is

a

precision

optical

instrument,

so

handle

it

carefully

and

do

not

subject

it

to

a

strong

physical

shock.

(The

accuracy

of

the

objective

in

particular

may

be

adversely

affected

by

even

a

weak

physical

shock.)

CONTENTS

Warning/Caution

Symbols

Used

in

This

Manual

...

ÆA

WARNING

..

A

CAUTION

il

Names

of

Component

Parts

and

Operational

Parts

...

Microscopic

Procedure...

Operation

of

Each

Part

Ш

Coarse

and

fine

focus

knobs

1.

Rotation

of

the

coarse

and

fine

focus

knobs

and

vertical

movement

of

the

stage

..

2.

Adjusting

the

torque

of

the

с;

3.

Coarse

focus

stopper......

Trinocular

eyepiece

tube

1.

Optical

path

selection

2.

Vertical

tube

adapter...

3.

Optical

path

selection

lever

clicking

El

Diopter

adjustment

E

Interpupillary

distance

adjustment

В

Conden:

1.

Focusing

and

centering

.

2.

Condenser

aperture

diaphragm

Swing-out

the top

lens

of

the

condense:

Field

diaphragm

E

Filters

Auto-photo

switch

(for

photomicrography)

..............

Oil-immersion

operation

(using

an

oil-immersion

type

of

objective)...

Orientation

of

polarizing

plates

(Polarizer

and

Analyzer)

Focusing

and

centering

the

Bertrand

len:

P-CL

1/4)

&

tint

plate

Centering

the

objectives

..

Ш

Circular

graduated

stage

for

polarizing

microscope

Use

of

optional

accessories

(Sold

Separately).

1.

P-CS

Sénarmont

Compensator

2.

P-CQ

Quartz

wedge...

3.

Attachable

Mechanical

Stage

....

Assembly

...........

Troubleshooting

Tables

..

n

Care

and

Maintenance

...

Specifications

and

Standards

....

Names

of

Component

Parts

and

Operational

Parts

If

the

microscope

has

not

yet

been

assembled,

see

chapter

“4.

Assembly"

first

Polarizing

intermediate

tube

|

Bertrand

lens

centering

screw

Specimen

сір

Substage

Condenser

focus

knob

Condenser

clamp

screw

Coarse

torque

adjust-

ment

ring

(TORQUE)

_

Brightness

adjuster

(LAMP)

Auto-photo

switch

Auto-photo

voltage

selection

switch

Binocular

eyepiece

tube

.

Eyepiece

Diopter

_

adjustment

ring

Analyzer

slider

P-CL

1/4)

&

tint

plate

Revolving

nosepiece

Objective

centering

screws

Objective

Vernier

Stage

rotation

....

clamp

screw

Circular

graduated

stage

Stage

clamp

screw

...

Polarizer

(Bottom

of

the

condenser]

Orientation

plate

1.

Names

of

Component

Parts

and

Operational

Parts

Eyepiece

tube

Berrandlenstumet

—

З

CE

Bertrand

lens

focus

Intermediate

tube

clamp

screw

Condenser

top

lens

swing-out

knob

Condenser

aperture

scale

А

жа

Condenser

aperture.

diaphragm

ring

||

Swing-out

condenser

|

Condenser

centering

ja

PEE.

Field

lens

Field

diaphragm

ring

-

Tool

holder

|

РИТЕ

(Но

а

hexagonal

screwdrver

and

two

centering

screws)

Lamp

cover

Se

Power

cord

(Rear)

Microscopic

Procedure

The

general

procedure

for

microscopy

is

described

below.

For

del

ils

on

each

step,

refer

to

the

orresponding

item

in

chapter

“3.

Operation

of

Басі

s

р!

If

the

microscope

has

not

yet

been

assembled,

see

chapter

“4.

Assembly"

first

Turn

on

the

power.

(Flip

the

switch

to

the

“17

side.)

As

soon

as

the

power

comes

on,

the

switch

lights.

Set

the

brightness

adjuster

to

give

B

the

desired

brightness.

Pressing

the

auto-photo

switch

will

set

the

voltage

for

the

brightness

that

provides

the

best

color

reproduction.

(p.21)

Insert

filters

NCBI

1

into

the

optical

path.

NCBI1

improves

color

reproduction.

(p.20)

Push

in

the

analyzer

slider

of

the

intermediate

tube

to

remove

the

analyzer

from

the

optical

path.

Put

the

Bertrand

lens

turret

in

the

Bertrand

lens

from

the

optical

path

E

Flip

the

switch

to

the

“|

"

side.

Insert

filters.

E

Adjust

the

brightness.

Auto-photo

switch

can

be

pressed.

Push

in

the

analyzer

slider.

Turn

the

turret

to

the

"O"

position.

~