NPI ISO-STIM 01B Istruzioni operative

OPERATING INSTRUCTIONS AND

SYSTEM DESCRIPTION FOR THE

ISO-STIM 01B

STIMULUS ISOLATOR

VERSION 1.8

npi 2016

npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany

Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240

[email protected]; http://www.npielectronic.com

ISO-STIM 01B User Manual

version 1.8 page 2

Table of Contents

1.Safety Regulations.............................................................................................................. 3

2.Special Safety Notice for High Voltage Instruments.......................................................... 4

3.ISO-STIM 01 B .................................................................................................................. 5

3.1.System Description...................................................................................................... 5

3.2.Description of the Front Panel..................................................................................... 5

3.3.Description of the Rear Panel...................................................................................... 8

3.4.ISO-CAB (optional accessory).................................................................................... 8

3.5.Operation..................................................................................................................... 9

3.5.1.Connections – Noise Reduction..................................................................... 9

3.5.2.Operation Modes............................................................................................ 9

4.Power Supply...................................................................................................................... 10

5.Technical Data ISO-STIM 01B.......................................................................................... 11

ISO-STIM 01B User Manual

version 1.8 page 3

1. Safety Regulations

VERY IMPORTANT:Instruments and components supplied by npi electronic are NOT

intended for clinical use or medical purposes (e.g. for diagnosis or treatment of humans),

or for any other life-supporting system. npi electronic disclaims any warranties for such

purpose. Equipment supplied by npi electronic must be operated only by selected, trained

and adequately instructed personnel. For details please consult the GENERAL TERMS

OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic, D-71732 Tamm,

Germany.

1) GENERAL: This system is designed for use in scientific laboratories and must be operated

by trained staff only. General safety regulations for operating electrical devices should be

followed.

2) AC MAINS CONNECTION: While working with the npi systems, always adhere to the

appropriate safety measures for handling electronic devices. Before using any device

please read manuals and instructions carefully.

The device is to be operated only at 115/230 Volt 60/50 Hz AC. Please check for

appropriate line voltage before connecting any system to mains.

Always use a three-wire line cord and a mains power-plug with a protection contact

connected to ground (protective earth).

Before opening the cabinet, unplug the instrument.

Unplug the instrument when replacing the fuse or changing line voltage. Replace fuse only

with an appropriate specified type.

3) STATIC ELECTRICITY: Electronic equipment is sensitive to static discharges. Some

devices such as sensor inputs are equipped with very sensitive FET amplifiers, which can

be damaged by electrostatic charge and must therefore be handled with care. Electrostatic

discharge can be avoided by touching a grounded metal surface when changing or

adjusting sensors. Always turn power off when adding or removing modules,

connecting or disconnecting sensors, headstages or other components from the

instrument or 19” cabinet.

4) TEMPERATURE DRIFT / WARM-UP TIME: All analog electronic systems are sensitive

to temperature changes. Therefore, all electronic instruments containing analog circuits

should be used only in a warmed-up condition (i.e. after internal temperature has reached

steady-state values). In most cases a warm-up period of 20-30 minutes is sufficient.

5) HANDLING: Please protect the device from moisture, heat, radiation and corrosive

chemicals.

ISO-STIM 01B User Manual

version 1.8 page 4

2. Special Safety Notice for High Voltage Instruments

HIGH VOLTAGE!!

RISK OF ELECTROCUTION!!

Observe extreme caution when working with this instrument!!!

1) Always connect high voltage power supplies to protective earth!!

2) Do not touch connections unless the instrument is turned off and the capacitance of both

the load and power supply are earthed!!

3) Allow adequate time for discharge of internal capacitance of the power supply!!

4) Do not ground yourself or work under wet or damp conditions!!

5) Servicing should be only done by qualified personnel aware of the hazards!!

6) If in doubt, return to supplier for servicing!!

ISO-STIM 01B User Manual

version 1.8 page 5

3. ISO-STIM 01 B

3.1.System Description

The ISO-STIM 01B stimulus isolation unit is designed for application of extracellular stimuli,

e.g. in brain slices.

The output signal is optically isolated from ground and can either be a constant voltage up to

100 V or a constant current up to 10 mA. A TTL signal at the TRIGGER INPUT generates

one stimulation pulse with adjustable pulse length, amplitude and polarity. An additional

DIRECT INPUT allows the pulse length to be externally controlled, whereas amplitude and

polarity are controlled by the device itself.

For both timing modes the generated pulses can be unipolar or bipolar. In bipolar mode an

interphase gap (IPG) of adjustable length can be inserted between the two pulses.

3.2.Description of the Front Panel

Figure 1: Front panel view of ISO-STIM 01B

In the following description of the front panel elements each element has a number that is

related to that in Figure 1. The number is followed by the name (in uppercase letters) written

on the front panel and the type of the element (in lowercase letters). Then, a short description

of the element is given.

ISO-STIM 01B User Manual

version 1.8 page 6

(1) POWER LED

LED indicating that the ISO-STIM 01D is powered on.

(2) POWER ON/OFF switch

Switch to turn POWER of the ISO-STIM 01D ON or OFF, respectively.

(3) DURATION (ms) digital potentiometer

Potentiometer that sets the duration of the stimulus generated by the built-in timing unit.

(4) OPERATION MODE (BIPOLAR/UNIPOLAR) switch

(5) AMPLITUDE 10-turn potentiometer

Potentiometer that sets the amplitude of the stimulus generated by the internal timing unit. The

maximum range is selected with the OUTPUT rotary switch (#15).

(6) +/- POLARITY switch

Switch for setting the polarity of the stimulus.

(7) OVER + LED

LED indicating that the amplifier is 10% below its positive limit (limit is ±100 V in VOLTAGE

OUTPUT MODE). In CURRENT OUTPUT MODE the limit (±100 V) is set by the

corresponding voltage at the electrode due to Ohm’s Law [V = I*R]), i.e. this control indicates

during an experiment in CURRENT OUTPUT MODE that the electrode resistance increases

and the amplifier is not be able to provide the current which is set if the electrode resistance

increases further.

(8) STIM LED

LED indicating the application of a voltage or current stimulus.

(9) OVER – LED

See (#7).

(10) CAP. COMP trim pot

The CAP.COMP. potentiometer compensates for the input capacitances in isolated CURRENT

output operation only. Capacity compensation is achieved by turning the potentiometer with a

small screw driver clockwise, until the current signal at the oscilloscope is as square as possible.

Important: If the CAP.COMP. is overcompensated in CURRENT MODE, the stimulus isolator

will ring and not work properly!

(11) CHASSIS connector

Jack linked to the CHASSIS. The green banana connector of the optional cable (ISO-CAB) is

connected here.

ISO-STIM 01B User Manual

version 1.8 page 7

(12) ISOLATED OUTPUT plug (negative)

The ISOLATED OUTPUT signal is available at two plugs (red and black). The polarity of the

+/- POLARITY switch (#6) is not changed, i.e. the red plug is positive (+) if the output polarity

switch is set to +. This signal is completely isolated from ground. The black jack serves as

reference point for the isolated output signal. The black banana connector of the supplied cable

is connected to the black jack and the red to the red jack.

Caution: THIS INSTRUMENT HAS A HIGH VOLTAGE OUTPUT (UP TO 100 V)!!!

Do not touch these pins or bare wires connected to these pins. Always turn power off if you

manipulate devices connected to these pins.

(13) ISOLATED OUTPUT plug (positive)

See (#12).

(14) OFFSET trim pot

Trim-pot to compensate for the OFFSET of the stimulating electrode. It is recommended to

compensate the offset only in a completely warmed up condition i.e. after 30 minutes warm-up

time. Compensation procedure is also possible in current mode.

(15) OUTPUT rotary switch

Switch for selecting current or voltage stimulation and for setting the maximum range to be set

with the AMPLITUDE potentiometer (#5).

(16) IPG TIME (µs) potentiometer with switch

Potentiometer to select the interphase gap time (IPG) in bipolar stimulation mode (see 3.5.2).

IPG is turned off in the leftmost position (switch).

(17) DIRECT INPUT (TTL) BNC connector

A TTL signal can be fed in here to trigger the output of the ISO-STIM-01D. Amplitude will be

set by #5,#6,#15. In unipolar mode (#4) the output will be active as long as a TTL HIGH signal

is fed into this input. In bipolar mode the output will be active for the time a TTL HIGH signal

is fed into this input. After this time an additional pulse is generated at the output with the same

duration and amplitude but with opposite polarity. The time between these two pulses is set by

the IPG TIME potentiometer (#16).

Important: For bipolar mode to work correctly with DIRECT INPUT TTL, the pulse length

should not exceed 100 ms. Also, the pause between two pulses must have at least the length of

the pulse plus the set IPG time.

(18) TRIGGER INPUT (TTL) BNC connector

A TTL signal can be fed in here to trigger the internal timing unit. Duration of the output signal

is set by the DURATION potentiometer (#3). Amplitude is set by #5,#6,#15.

(19) MANUAL TRIGGER pushbutton switch

This pushbutton has the same functionality as the TTL input (#18), but is for manual operation.

ISO-STIM 01B User Manual

version 1.8 page 8

3.3.Description of the Rear Panel

Figure 2: Rear panel view of ISO-STIM 01B

On the rear panel of the ISO-STIM 01B there is only one SubD connector. This connector is

used to connect the ISO-STIM 01B to its power supply.

3.4.ISO-CAB (optional accessory)

npi provides an optional shielded cable for connection of stimulation electrodes. One end is

equipped with three color coded 4 mm connectors. The red and black connector are connected

to the ISOLATED OUTPUT connectors on the front panel of the ISO-STIM-01 D. Each of

these stimulation lines has a shield which is connected to the common green SHIELD

connector. To reduce noise during recordings, this connector can be connected to the CHASSIS

connector at the ISO-STIM-01 D front panel

The other end of the cable provides open wires, to which different connector types can be

soldered by the user. The red cable is +STIM, the yellow cable is –STIM. The shield is present

as bare wire. Usually, these shield wires are left unconnected on this end of the cable.

Figure 3: ISO-CAB - shielded cable for connection of stimulation electrodes.

ISO-STIM 01B User Manual

version 1.8 page 9

3.5.Operation

3.5.1. Connections – Noise Reduction

The ISO-STIM 01B comes with a power supply with SubD cable, which is connected to the

stimulus isolation device. The corresponding connector at the ISO-STIM 01B is located at the

rear panel.

Note: A possible source of noise is the chassis of the stimulator. In a noisy environment, it may

act as an antenna picking up 50 Hz or 60 Hz noise respectively, especially when operating with

current stimuli. In this case, the noise is substantially reduced if the chassis is connected to

ground (see also #11, Figure 1).

The stimulation electrode is connected to the isolated output (see Figure 1). We recommend to

use a bipolar electrode for stimulating nerve bundles. This will produce only a short stimulation

artifact at the beginning.

Remember: If unipolar electrodes are used the whole slice will be stimulated resulting in a DC

shift of the whole slice.

3.5.2. Operation Modes

BIPOLAR:

The isolator is capable to generate biphasic stimuli by switching the stimulus mode switch to

BIPOLAR ON. In this mode, the stimulus that is set by the user will be followed by a second

stimulus with the same amplitude and duration, but with reverse polarity. There is no delay

between first and second part of the stimulus (except IPG is active, see below). For instance, if

the user sets a positive stimulus of +1 V amplitude and 100 µs duration, this stimulus will be

followed immediately by a negative stimulus of –1 V and 100 µs duration.

BIPOLAR with IPG:

In bipolar mode, a delay between the first and the second, inverse stimulus can be inserted, the

interphase gap (IPG, see Figure 4). IPG time is set by (#16)

Figure 4: Interphase gap (IPG)

OPERATION IN THE 10 mA (max.) RANGE

According to Ohm’s law, with 10 V voltage 10 mA current can be achieved only if the

resistance of the stimulus electrode is not higher than 1 k. Otherwise, the current will be less.

ISO-STIM 01B User Manual

version 1.8 page 10

4. Power Supply

The ISO-STIM 01B is powered with a special power supply (see Figure 5). The power supply

is connected to the ISO-STIM 01B with the provided 9 line SubD cable. It will only fit in one

direction.

Figure 5: Front panel (left) and rear panel (right) view of the ISO-STIM 01B power supply

The power supply is connected to mains with a standard power cord. It has a POWER ON

switch on the rear panel.

Important: Only use the supplied power supply to power the ISO-STIM 01B. Using different

power supplies might result in damage of ISO-STIM 01B and/or power supply.

Important: This power supply is designed for use with the ISO-STIM 01B only. Using it

differently might result in damage of the power supply.

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